Genetic characterization of Vimba vimba persa (Pallas, 1814) in southern parts of the Caspian Sea using microsatellite markers

Population genetic structure of Vimba vimba persa was investigated using microsatellite markers from 40 regions along the Iranian coastline of the Southern Caspian Sea (Anzali lagoon and Havigh River in Gilan province, BabolRoud River in Mazandaran province and GorganRoud River in Golestan province). Genomic DNA from 121 specimens was extracted from fin tissue by the Phenol-Chlorophorm method and PCR reaction was accomplished with 17 microsatellite primers, out of 17 microsatellite primers 13 loci were amplified, in which 10 of them were amplified with reasonable polymorphism and 3 were monomorphism. A total of 302 alleles were identified on average 7.55. Observed and expected heterozygosity averages were 0.80 and 0.77 respectively. Most cases significantly deviated from Hardy-Weinberg equilibrium (p≤0.01). The estimation of Fst (p≤0.01) revealed significant population structuring and an estimation of the four population of Vimba vimba persa was identified in the Caspian Sea in which restocking of these species should be considered

О закономерностях распределения гелия в осадочном чехле юго-востока Беларуси

In this work, microscopic and histological studies suggest that Strobilanthes crispus ethanol extract reduce azoxymethane (AOM)-induced colonic aberrant crypt foci (ACF) in rats. S. crispus is considered a traditional medicine and used as an antioxidant. Its leaf contains a large amount of phenolic compounds to which its radical scavenging role is attributed and enhance its ability to eradicate oxidative stress reactions. The study was designed to determine the chemopreventive effect of S. crispus ethanol extract in vivo and in vitro by elucidating the effect of the extract on intermediate biomarkers which can be used as effective predictors of colon cancer. S. crispus was analyzed for DPPH free radical scavenging, nitric oxide (NO) and ferric acid reduction. The results indicated that S. crispus oral administration significantly inhibited colorectal carcinogenesis induced by AOM as revealed by the reduction in the number of ACF. S. crispus down-regulated the expression of PCNA, Bcl2 and beta-catenin. Additionally, it exerted a pronounced inhibitory effect on MDA and NO levels and stimulatory effect on CAT and GPx activities. These results demonstrate that S. crispus is a chemopreventive agent for colorectal cancer through the suppression of early and intermediate carcinogenic phases that may be related to its flavonoid content

Analysis of middle-to-far wake behind floating offshore wind turbines in the presence of multiple platform motions

Understanding the unsteady characteristics of the mid-to-far wake and comprehension of the aerodynamic performance of floating offshore wind turbines is essential for the further development of offshore wind farms. In this perspective, a developed Actuator line model is utilized to analyze an offshore turbine on four different platforms. The model reliability was examined through three sets of validations involving turbine output in fixed and floating conditions and wake expansion in terms of size and rate. The affected relative velocity by the platform motion contributes to the wake deformation and temporal effects on induced velocity. Angular platform motions produce a non-axisymmetric helical wake that raises the chance of meandering wake patterns. It was founded although platform movement generally can boost the recovery of mean velocity value, it may amplify the amplitude of velocity deficit fluctuation at further downstream by encouraging interactions and merging vortex rings. Consequently, the wake propagates into a form of stronger circles whose period, strength, and center are functions of turbine movement and operation conditions. By providing a computationally efficient tool, the findings emphasize the importance of wake propagation in designing and assessing farm layouts that operate in the presence of significant multi-motions of floating offshore wind turbines

Pyrolytic carbon film deposit as an electrochemical interface.

A pyrolytic carbon (PC) film was grown on planar substrate (graphite rods) by chemical vapor deposition from gaseous feed of methane using a vertical hot-wall deposition reactor. Scanning electron microscopy was used to study the surface structure. The PC film was also characterized by cyclic voltammetry technique to evaluate the background current, stability and the electrochemical response using ascorbic acid, Co(phen)32+/3+ and Fe(CN)6 3-/4- redox couplesand compared to glassy carbon (GC) electrode. High degree of electrochemical activity and the enhanced signal to background (S/B) ratio demonstrated that the PC film might be an attractive electrode material for electroanalytical measurements

Multiwavelength spectrophotometric determination of protolytic constants of 4-(2-pyridylazo)resorcinol (PAR) in binary dioxane + water mixtures

Acid-base properties of 4-(2-pyridylazo)resorcinol (PAR) in 1,4-dioxane (DX)-water binary mixture were investigated using a multiwavelength spectrophotometric method. The protolytic equilibrium constants, spectral profiles, concentration diagrams and also the number of components have been calculated from the fitting of the pH-absorbance data with appropriate mass balance equations by an established factor analysis model. The binary mixture of water-DX is a good system to study changing in the donating ability and permittivity of mixed solvent components in comparison to each other, considering the fact that variation in the permittivity from pure water to pure DX is 76 units. A linear relationship is observed between pKa\u27s of the PAR molecule and the mole fraction of DX in different solvent mixtures. A glass electrode calibration procedure based on a four-parameter equation aided Gran\u27s method was used to obtain pH readings based on the concentration scale (p cH). It has been observed that there is an inverse relationship between second and third protolytic constants and mole fraction of DX. The effect of the solvent on the protolytic constants was discussed

Chemopreventive effects of Strobilanthes crispus leaf extract on azoxymethane-induced aberrant crypt foci in rat colon

In this work, microscopic and histological studies suggest that Strobilanthes crispus ethanol extract reduce azoxymethane (AOM)-induced colonic aberrant crypt foci (ACF) in rats. S. crispus is considered a traditional medicine and used as an antioxidant. Its leaf contains a large amount of phenolic compounds to which its radical scavenging role is attributed and enhance its ability to eradicate oxidative stress reactions. The study was designed to determine the chemopreventive effect of S. crispus ethanol extract in vivo and in vitro by elucidating the effect of the extract on intermediate biomarkers which can be used as effective predictors of colon cancer. S. crispus was analyzed for DPPH free radical scavenging, nitric oxide (NO) and ferric acid reduction. The results indicated that S. crispus oral administration significantly inhibited colorectal carcinogenesis induced by AOM as revealed by the reduction in the number of ACF. S. crispus down-regulated the expression of PCNA, Bcl2 and beta-catenin. Additionally, it exerted a pronounced inhibitory effect on MDA and NO levels and stimulatory effect on CAT and GPx activities. These results demonstrate that S. crispus is a chemopreventive agent for colorectal cancer through the suppression of early and intermediate carcinogenic phases that may be related to its flavonoid content