Explant-derived human dental pulp stem cells enhance differentiation and proliferation potentials

Numerous stem cell niches are present in the different tissues and organs of the adult human body. Among these tissues, dental pulp, entrapped within the 'sealed niche' of the pulp chamber, is an extremely rich site for collecting stem cells. In this study, we demonstrate that the isolation of human dental pulp stem cells by the explants culture method (hD-DPSCs) allows the recovery of a population of dental mesenchymal stem cells that exhibit an elevated proliferation potential. Moreover, we highlight that hD-DPSCs are not only capable of differentiating into osteoblasts and chondrocytes but are also able to switch their genetic programme when co-cultured with murine myoblasts. High levels of MyoD expression were detected, indicating that muscle-specific genes in dental pulp cells can be turned on through myogenic fusion, confirming thus their multipotency. A perivascular niche may be the potential source of hD-DPSCs, as suggested by the consistent Ca(2+) release from these cells in response to endothelin-1 (ET-1) treatment, which is also able to significantly increase cell proliferation. Moreover, response to ET-1 has been found to be superior in hD-DPSCs than in DPSCs, probably due to the isolation method that promotes release of stem/progenitor cells from perivascular structures. The ability to isolate, expand and direct the differentiation of hD-DPSCs into several lineages, mainly towards myogenesis, offers an opportunity for the study of events associated with cell commitment and differentiation. Therefore, hD-DPSCs display enhanced differentiation abilities when compared to DPSCs, and this might be of relevance for their use in therapy

The CUORE cryostat: an infrastructure for rare event searches at millikelvin temperatures

The CUORE experiment is the world's largest bolometric experiment. The detector consists of an array of 988 TeO2 crystals, for a total mass of 742 kg. CUORE is presently taking data at the Laboratori Nazionali del Gran Sasso, Italy, searching for the neutrinoless double beta decay of 130Te. A large custom cryogen-free cryostat allows reaching and maintaining a base temperature of about 10 mK, required for the optimal operation of the detector. This apparatus has been designed in order to achieve a low noise environment, with minimal contribution to the radioactive background for the experiment. In this paper, we present an overview of the CUORE cryostat, together with a description of all its sub-systems, focusing on the solutions identified to satisfy the stringent requirements. We briefly illustrate the various phases of the cryostat commissioning and highlight the relevant steps and milestones achieved each time. Finally, we describe the successful cooldown of CUORE

Disulfide relays and phosphorylative cascades: Partners in redox-mediated signaling pathways

Modifications of specific amino-acid residues of proteins are fundamental in order to modulate different signaling processes among which the cascade of phosphorylation represents the most effective example. Recently, also, the modification of the redox state of cysteine residues of certain proteins, which is a widespread mechanism in the regulation of protein function, has been proposed to be involved in signaling pathways. Growing evidence shows that some transcription factors could be modulated by both oxidation and phosphorylation. In particular, the pathways regulated by the mitogen activated protein (MAP) kinases represent well-established examples of the cross talk between redox-mediated signaling and phosphorylative cascades. This review will compare the two modes of signal transduction and propose an evolutionary model of a partnership of the two mechanisms in the eukaryotic cell, with redox-mediated signals being more specific and ancestral and phosphorylative signals being more diffuse but predominant in signal propagation. © 2005 Nature Publishing Group All rights reserved

Framework for Computerizing the Processes of a Job and Automating the Operational Management on Site—A Case Study of Demolition and Reconstruction Construction Site

With regard to the topic of digitization for operational site control, it is clear that while there is an acceleration towards digitization of processes, especially complex ones, there are still clear research gaps to be filled. The present study is part of this line of research and aims to respond to the current demand for digital and sustainable innovation of production processes. It, therefore, aims at defining a framework for the management of an order that takes place in real time. In order to improve the quality of information and organizational processes of an order in a project-oriented logic, a framework has been created: Model Smart Management. The framework integrates project management techniques with digital innovation tools and includes a computerized procedure to monitor and control the operational management of the site: SmartApp. In order to allow the interoperability of information, the platform can be used entirely in the cloud. From the processing of data in the planning phase, ad hoc checklists are developed that include a series of activities closely related to each other to ensure a continuity step by step, allowing users to have an immediate control of the project and of the relevant key performance indicators thanks to an advanced and fully customizable reporting. SmartApp, integrated with a cloud compu-ting system, makes computerized and automated the operational control on site, creating a centralized archive of all company data, reducing time and costs for the management of documentation. Moreover, with the omnichannel system, paper documents are managed and archived through QR-code, optical character recognition on smart devices, so as to achieve a regime of maximum efficiency. This framework was then applied in a case study located in L'Aquila, Italy, repre-sented by a post 2009 earthquake demolition and reconstruction site

Energy transitions in Western European Countries: Regulation comparative analysis

Shifting from fossil to renewable energy sources is a major global challenge, and in this context, the European Union has promoted sustainable and environmentally friendly growth as early as the Maastricht Treaty of 1992. To date, European institutions have promulgated a series of environmental regulations and directives aimed at promoting and imposing adoption by member states of internal regulations. This paper is focused on Western Europe, and it explores, for each state under analysis, energy policies adopted, the results achieved and recommendations for the future growth of renewable energy. The results show that in countries where energy policy is not fragmented, the yield in renewable energies has been higher, and also in the states where more and various forms of subsidies are foreseen, growth seems to be greater. Finally, the paper provides useful recommendations and future policy implications for states that have not met the 2020 targets

Renovation wave: A bioeconomy panel produced with waste

Achieving the 2050 carbon neutrality targets calls for change, especially in what are identified as the most energy-intensive production sectors. Among these, the construction industry sector certainly requires special attention to promote sustainable and more circular actions. Moreover, the so-called green transition is considered the same as an additional European goal, namely that of the digital transition. The research presented in this article falls precisely within these two fields of study. It illustrates the process and methodological approach developed to produce a new panel for use in construction. This panel is made with Hemp-Pla, where hemp comes from the residues of threshing leaves, and is printed with additive technology. The achievement of the final result has required the elaboration of six prototypes, differentiated from each other according to the geometry of the section, the thickness, and the infill. The development of several prototypes made it possible to identify the main printing difficulties and the time and amount of material required. Based on the knowledge gained during the printing process of these prototypes and the analyses aimed at knowing their main thermal performance, it was possible to design and create a commercial-scale panel. Its energy performance values are comparable to panels already made from traditional materials. In addition, the Life Cycle Assessment showed that the results obtained are auspicious for bringing the printed panels made from Hemp-Pla to the construction industry market

High-resolution bolometers for rare events detection

Since many years the Milano Gran Sasso collaboration is developing large mass calorimeters for Double Beta Decay and Dark Matter searches, employing TeO2 crystals as absorber elements. Recently, we have focused our attention on the improvement of the detector resolution: an efficient dumping suspension and the implementation of a new cold electronics device, have strongly suppressed the main sources of noise. The increase in SIN ratio has been of almost an order of magnitude and the resolution achieved is competitive with that of Ge diodes for gamma -rays detection, while a FWHM of 3.2 +/-0.3 keV has been obtained for 5.4 MeV alpha particles, the best result with any kind of detector. (C) 2001 Elsevier Science B.V. All rights reserve