Technology Transfer in Forest Pest Management: A Case History

The current approach being used in the spruce budworm technology transfer program for the Lake States is described. During 1981-1982, we concentrated on needs assessment surveys and the development and packaging of materials in five areas: general manual, chemical control handbook, silviculture handbook, instruction manual for remote sensing workshops, and technical reports on budworm impact on spruce-fir stands. We present a list of factors that researchers and technology transfer specialists should consider when plan- ning a research and technology transfer program in forest pest management

Higgs look-alikes at the LHC

The discovery of a Higgs particle is possible in a variety of search channels at the LHC. However the true identity of any putative Higgs boson will at first remain ambiguous, until one has experimentally excluded other possible assignments of quantum numbers and couplings. We quantify to what degree one can discriminate a Standard Model Higgs boson from "look-alikes" at, or close to, the moment of discovery at the LHC. We focus on the fully-reconstructible "golden" decay mode to a pair of Z bosons and a four-lepton final state, simulating sPlot-weighted samples of signal and background events. Considering both on-shell and off-shell Z's, we show how to utilize the full decay information from the events, including the distributions and correlations of the five relevant angular variables. We demonstrate how the finite phase space acceptance of any LHC detector sculpts the decay distributions, a feature neglected in previous studies. We use likelihood ratios to discriminate a Standard Model Higgs from look-alikes with other spins or nonstandard parity, CP, or form factors. For a benchmark resonance mass of 200 GeV/c^2, we achieve a median expected discrimination significance of 3 sigma with as few as 19 events, and even better discrimination for the off-shell decays of a 145 GeV/c^2 resonance.Comment: 39 pages, 55 figures, typos fixed, figures added, and minor clarification

Does hydatid cyst fluid from Echinococcus granulosus cysts have any effect on cells involved in fibrosis in cystic echinococcosis?

Cystic echinococcosis is characterized by the presence of slow growing hydatid cysts, usually in the liver or lungs. Survival of the parasite is based on an interaction of the host immune system and a range of parasite immune-evasive strategies. Fibrosis in the tissues surrounding the cysts can be seen as a host protective response isolating the parasite and restricting its growth or from another perspective fibrosis may be protective for the parasite by providing a barrier to more effective immunological responses. In this study the adenocarcinomic human alveolar basal epithelial cell line (A549) was used as model system. This cell line can be involved in fibrosis as cells can transform into mesenchymal cells and differentiate later to fibroblasts and/or myofibroblasts which can ultimately secrete collagen. Cells were initially cultured in vitro in RPMI-1640 medium containing 1-10% hydatid cyst fluid (HCF). The possible effect of the parasite extracts on cell migration was investigated using a wound healing assay. The ability of HCF components to modify cell surface markers of mesenchymal transition was also investigated by fluorescence microscopy. Results showed that there was a dose-dependent increase in cell growth in the presence of cyst fluid after 5 days of culture. The migratory response of cells was also enhanced by the presence of HCF. Both the enhanced growth and migratory activity were still evident when the HCF had been boiled indicating that the components responsible were thermostable. Semi-purified extracts of a major HCF component, antigen B showed a similar high stimulatory effect similar to that of HCF. The fluorescence microscopy showed a significant expression in the fibronectin and E-cadherin cell markers in cells treated with HCF. These results indicate that components within HCF have a stimulatory effect in the possible enhancement of fibrosis

Future Circular Collider Conceptual Design Report Volume 1

This work is licensed under a Creative Commons Attribution 4.0 International License.We review the physics opportunities of the Future Circular Collider, covering its e+e-, pp, ep and heavy ion programmes. We describe the measurement capabilities of each FCC component, addressing the study of electroweak, Higgs and strong interactions, the top quark and flavour, as well as phenomena beyond the Standard Model. We highlight the synergy and complementarity of the different colliders, which will contribute to a uniquely coherent and ambitious research programme, providing an unmatchable combination of precision and sensitivity to new physics

21st century fisheries management: a spatio-temporally explicit tariff-based approach combining multiple drivers and incentivising responsible fishing

Abstract Kraak, S. B. M., Reid, D. G., Gerritsen, H. D., Kelly, C. J., Fitzpatrick, M., Codling, E. A., and Rogan, E. 2012. 21st century fisheries management: a spatio-temporally explicit tariff-based approach combining multiple drivers and incentivising responsible fishing. – ICES Journal of Marine Science, 69: 590–601. Traditionally fisheries management has focused on biomass and mortality, expressed annually and across large management units. However, because fish abundance varies at much smaller spatio-temporal scales, fishing mortality can potentially be controlled more effectively if managed at finer scale. The ecosystem approach requires more indicators at finer scales as well. Incorporating ecosystem targets would need additional management tools with potentially conflicting results. We present a simple, integrated, management approach that provides incentives for “good behaviour”. Fishers would be given a number of fishing-impact credits, called real-time incentives (RTIs), to spend according to spatio-temporally varying tariffs per fishing day. RTI quotas and tariffs could be based on commercial stocks and ecosystem targets. Fishers could choose how to spend their RTIs, e.g. by limited fishing in high-catch or sensitive areas or by fishing longer in lower-catch or less sensitive areas. The RTI system does not prescribe and forbid, but instead allows fishers to fish wherever and whenever they want; ecosystem costs are internalized and fishers have to take them into account in their business decisions. We envisage no need for traditional landings or catch quotas for the fleets while operating under the scheme. The approach could facilitate further devolution of responsibility to industry.</jats:p

Application of decomposition techniques to the preliminary design of a transport aircraft

A nonlinear constrained optimization problem describing the preliminary design process for a transport aircraft has been formulated. A multifaceted decomposition of the optimization problem has been made. Flight dynamics, flexible aircraft loads and deformations, and preliminary structural design subproblems appear prominently in the decomposition. The use of design process decomposition for scheduling design projects, a new system integration approach to configuration control, and the application of object-centered programming to a new generation of design tools are discussed

Localized, Non-Random Differences in Chromatin Accessibility Between Homologous Metaphase Chromosomes

BACKGROUND: Condensation differences along the lengths of homologous, mitotic metaphase chromosomes are well known. This study reports molecular cytogenetic data showing quantifiable localized differences in condensation between homologs that are related to differences in accessibility (DA) of associated DNA probe targets. Reproducible DA was observed for ~10% of locus-specific, short (1.5-5 kb) single copy DNA probes used in fluorescence in situ hybridization. RESULTS: Fourteen probes (from chromosomes 1, 5, 9, 11, 15, 17, 22) targeting genic and intergenic regions were developed and hybridized to cells from 10 individuals with cytogenetically-distinguishable homologs. Differences in hybridization between homologs were non-random for 8 genomic regions (RGS7, CACNA1B, GABRA5, SNRPN, HERC2, PMP22:IVS3, ADORA2B:IVS1, ACR) and were not unique to known imprinted domains or specific chromosomes. DNA probes within CCNB1, C9orf66, ADORA2B:Promoter-Ex1, PMP22:IVS4-Ex 5, and intergenic region 1p36.3 showed no DA (equivalent accessibility), while OPCML showed unbiased DA. To pinpoint probe locations, we performed 3D-structured illumination microscopy (3D-SIM). This showed that genomic regions with DA had 3.3-fold greater volumetric, integrated probe intensities and broad distributions of probe depths along axial and lateral axes of the 2 homologs, compared to a low copy probe target (NOMO1) with equivalent accessibility. Genomic regions with equivalent accessibility were also enriched for epigenetic marks of open interphase chromatin (DNase I HS, H3K27Ac, H3K4me1) to a greater extent than regions with DA. CONCLUSIONS: This study provides evidence that DA is non-random and reproducible; it is locus specific, but not unique to known imprinted regions or specific chromosomes. Non-random DA was also shown to be heritable within a 2 generation family. DNA probe volume and depth measurements of hybridized metaphase chromosomes further show locus-specific chromatin accessibility differences by super-resolution 3D-SIM. Based on these data and the analysis of interphase epigenetic marks of genomic intervals with DA, we conclude that there are localized differences in compaction of homologs during mitotic metaphase and that these differences may arise during or preceding metaphase chromosome compaction. Our results suggest new directions for locus-specific structural analysis of metaphase chromosomes, motivated by the potential relationship of these findings to underlying epigenetic changes established during interphase

The Impact of Tree Planting Program Governance Structure on Tree Survivorship and Vigor: A Case Study using the Massachusetts Greening the Gateway Cities Program

Trees in urban neighborhoods benefit residents by reducing building energy costs, providing cleaner air, decreasing surface runoff, and improving quality of life. However, tree canopy cover is not evenly distributed across neighborhoods in many mid-sized American cities which leads to higher air and surface temperatures, and increased energy bills for residents who are the most economically vulnerable. The state of Massachusetts (USA) created the Greening the Gateway Cities (GGC) program to increase tree canopy cover by 10% in post-industrial, midsized cities with lower educational attainment and lower income than state averages. The study posed two questions: what is the governance structure of the GGC program? How successful is the program using annual survivorship and vigor of the trees? This research examines the GGC program as a case study for a governance structure that fosters connections between the city, community and residents can create the social and environmental infrastructure to support increased tree canopy in urban neighborhoods. Data was collected in four gateway cities in Massachusetts: Chicopee, Fall River, Holyoke and Chelsea. 49 residents who received trees as part of the program were interviewed as well as two DCR foresters, three city planners, one head of the city’s community maintenance (Department of Public Works), and two paid staff and three volunteers of community partners. These interviews informed the creation of a governance framework for the GGC program. Tree survivorship, annual mortality and vigor of 3459 trees were used to measure the initial success of the planting program and to forecast potential benefits to residents. Results show how the GGC planting program can produce increased sense of ownership between cities, communities and individuals in the planting zones. The governance model, with an emphasis on stewardship, showed high rates of annual survivorship (~96.5%), low annual mortality rates (~3.5%) and average vigor rating of 1.5 (1 being healthy, 5 being dead)