Study of the spring and autumn daemon-flux maxima at the Baksan Neutrino Observatory

Detection of daemons in low-background conditions in September 2005 and March 2006 has provided evidence for the expected to occur at that times maxima in the flux of daemons with V ~ 10-15 km s-1, which hit the Earth from near-Earth, almost circular heliocentric orbits. The ability of some FEU-167-1 PM tubes with a thicker inner Al coating to detect directly daemon passage through them has also been demonstrated, an effect increasing ~100-fold the detector efficiency. As a result, the daemon flux recorded at the maxima was increased from ~10-9 to ~10-7 cm-2 s-1. The intensity and direction of the flux during maxima depend on the time of day and latitude of observations (therefore, synchronous measurements in the Northern and Southern Earth's hemispheres are desirable). All the experimental results obtained either support the conclusions following from the daemon paradigm or find a simple interpretation within it.Comment: 15 pages, including 8 figures and 3 table

Off-limb EUV observations of the solar corona and transients with the CORONAS-F/SPIRIT telescope-coronagraph

The SPIRIT telescope aboard the CORONAS-F satellite (in orbit from 26 July 2001 to 5 December 2005), observed the off-limb solar corona in the 175 Å (Fe IX, X and XI lines) and 304 Å (He II and Si XI lines) bands. In the coronagraphic mode the mirror was tilted to image the corona at the distance of 1.1...5 <I>R</I><sub>sun</sub> from the solar center, the outer occulter blocked the disk radiation and the detector sensitivity was enhanced. This intermediate region between the fields of view of ordinary extreme-ultraviolet (EUV) telescopes and most of the white-light (WL) coronagraphs is responsible for forming the streamer belt, acceleration of ejected matter and emergence of slow and fast solar wind. We present here the results of continuous coronagraphic EUV observations of the solar corona carried out during two weeks in June and December 2002. The images showed a "diffuse" (unresolved) component of the corona seen in both bands, and non-radial, ray-like structures seen only in the 175 Å band, which can be associated with a streamer base. The correlations between latitudinal distributions of the EUV brightness in the corona and at the limb were found to be high in 304 Å at all distances and in 175 Å only below 1.5 <I>R</I><sub>sun</sub>. The temporal correlation of the coronal brightness along the west radial line, with the brightness at the underlying limb region was significant in both bands, independent of the distance. On 2 February 2003 SPIRIT observed an expansion of a transient associated with a prominence eruption seen only in the 304 Å band. The SPIRIT data have been compared with the corresponding data of the SOHO LASCO, EIT and UVCS instruments

Bioinformatic identification of novel putative photoreceptor specific cis-elements

<p>Abstract</p> <p>Background</p> <p>Cell specific gene expression is largely regulated by different combinations of transcription factors that bind <it>cis</it>-elements in the upstream promoter sequence. However, experimental detection of <it>cis</it>-elements is difficult, expensive, and time-consuming. This provides a motivation for developing bioinformatic methods to identify <it>cis</it>-elements that could prioritize future experimental studies. Here, we use motif discovery algorithms to predict transcription factor binding sites involved in regulating the differences between murine rod and cone photoreceptor populations.</p> <p>Results</p> <p>To identify highly conserved motifs enriched in promoters that drive expression in either rod or cone photoreceptors, we assembled a set of murine rod-specific, cone-specific, and non-photoreceptor background promoter sequences. These sets were used as input to a newly devised motif discovery algorithm called Iterative Alignment/Modular Motif Selection (IAMMS). Using IAMMS, we predicted 34 motifs that may contribute to rod-specific (19 motifs) or cone-specific (15 motifs) expression patterns. Of these, 16 rod- and 12 cone-specific motifs were found in clusters near the transcription start site. New findings include the observation that cone promoters tend to contain TATA boxes, while rod promoters tend to be TATA-less (exempting <it>Rho </it>and <it>Cnga1</it>). Additionally, we identify putative sites for IL-6 effectors (in rods) and RXR family members (in cones) that can explain experimental data showing changes to cell-fate by activating these signaling pathways during rod/cone development. Two of the predicted motifs (NRE and ROP2) have been confirmed experimentally to be involved in cell-specific expression patterns. We provide a full database of predictions as additional data that may contain further valuable information. IAMMS predictions are compared with existing motif discovery algorithms, DME and BioProspector. We find that over 60% of IAMMS predictions are confirmed by at least one other motif discovery algorithm.</p> <p>Conclusion</p> <p>We predict novel, putative <it>cis-</it>elements enriched in the promoter of rod-specific or cone-specific genes. These are candidate binding sites for transcription factors involved in maintaining functional differences between rod and cone photoreceptor populations.</p

ВИКОРИСТАННЯ СИМУЛЯЦІЙНИХ ТЕХНОЛОГІЙ ПРИ НАВЧАННІ СТУДЕНТІВ З НАДАННЯ ЕКСТРЕНОЇ МЕДИЧНОЇ ДОПОМОГИ

The aim of the study – to show new effective methods of teaching students that are embedded in the department of disaster medicine, military medicine, anesthesiology and intensive care ofZaporizhzhiaStateMedicalUniversity.Materials and methods. The main features of simulation training is the use dummies or extras to complete and realistic simulation of an object in a particular situation; working out specific practical skills using modern equipment without damage to human health; practicing teamwork in the imitated situation.Results and discussion. The introduction were driven to automatism algorithms of cardiopulmonary resuscitation; trauma management, drowning; initial triage issues.Conclusions. 1. Forms simulation training  system is highly practical training of medical students. 2. Team classes have priority form working algorithm cardiopulmonary resuscitation, trauma management. 3. Master classes should be used as part of practical training during scientific conferences. 4. Training seminars are the best form of training in working with non-medical audience (military personnel, volunteers, students). 5. Competitions help students improve practical skills to develop the skills of primary triage and more.Мета роботи – показати нові ефективні методи навчання студентів, які впроваджені на кафедрі медицини катастроф, військової медицини, анестезіології та інтенсивної терапії Запорізького державного медичного університету.Матеріали та методи. Головні ознаки симуляційного навчання: можливість використання манекенів або статистів для повноти та реалістичності моделювання об’єкта в певній ситуації; відпрацювання конкретних практичних навичок з використанням сучасної апаратури без завдання шкоди здоров’ю людини; відпрацювання командної роботи в імітованій конкретній ситуації.Результати й обговорення. Результатом впровадження стали доведені до автоматизму алгоритми проведення серцево-легеневої реанімації; надання допомоги при політравмі,  нефатальному втопленні; питання первинного медичного сортування.Висновки. 1. Форми симуляційного навчання (командні заняття, майстер-класи, семінари-тренінги, змагання) є високоефективною системою практичної підготовки студентів медичних вузів. 2. Командні заняття є пріоритетною формою відпрацювання алгоритму серцево-легеневої реанімації, надання екстреної медичної допомоги при травмі. 3. Майстер-класи слід використовувати як елемент практичної підготовки під час проведення науково-практичних конференцій. 4. Семінари-тренінги є оптимальною формою навчання у роботі з немедичною аудиторією (військовослужбовцями, волонтерами, школярами). 5. Змагання допомагають студентам вдосконалити практичні навички, оволодіти навичками первинного медичного сортування тощо

Theory of Spike Spiral Waves in a Reaction-Diffusion System

We discovered a new type of spiral wave solutions in reaction-diffusion systems --- spike spiral wave, which significantly differs from spiral waves observed in FitzHugh-Nagumo-type models. We present an asymptotic theory of these waves in Gray-Scott model. We derive the kinematic relations describing the shape of this spiral and find the dependence of its main parameters on the control parameters. The theory does not rely on the specific features of Gray-Scott model and thus is expected to be applicable to a broad range of reaction-diffusion systems.Comment: 4 pages (REVTeX), 2 figures (postscript), submitted to Phys. Rev. Let

Fiber Organization has Little Effect on Electrical Activation Patterns during Focal Arrhythmias in the Left Atrium

Over the past two decades there has been a steady trend towards the development of realistic models of cardiac conduction with increasing levels of detail. However, making models more realistic complicates their personalization and use in clinical practice due to limited availability of tissue and cellular scale data. One such limitation is obtaining information about myocardial fiber organization in the clinical setting. In this study, we investigated a chimeric model of the left atrium utilizing clinically derived patient-specific atrial geometry and a realistic, yet foreign for a given patient fiber organization. We discovered that even significant variability of fiber organization had a relatively small effect on the spatio-temporal activation pattern during regular pacing. For a given pacing site, the activation maps were very similar across all fiber organizations tested

Generation and escape of local waves from the boundary of uncoupled cardiac tissue

We aim to understand the formation of abnormal waves of activity from myocardial regions with diminished cell-to-cell coupling. In route to this goal, we studied the behavior of a heterogeneous myocyte network in which a sharp coupling gradient was placed under conditions of increasing network automaticity. Experiments were conducted in monolayers of neonatal rat cardiomyocytes using heptanol and isoproterenol as means of altering cell-to-cell coupling and automaticity respectively. Experimental findings were explained and expanded using a modified Beeler-Reuter numerical model. The data suggests that the combination of a heterogeneous substrate, a gradient of coupling and an increase in oscillatory activity of individual cells creates a rich set of behaviors associated with self-generated spiral waves and ectopic sources. Spiral waves feature a flattened shape and a pin-unpin drift type of tip motion. These intercellular waves are action-potential based and can be visualized with either voltage or calcium transient measurements. A source/load mismatch on the interface between the boundary and well-coupled layers can lock wavefronts emanating from both ectopic sources and rotating waves within the inner layers of the coupling gradient. A numerical approach allowed us to explore how: i) the spatial distribution of cells, ii) the amplitude and dispersion of cell automaticity, iii) and the speed at which the coupling gradient moves in space, affects wave behavior, including its escape into well-coupled tissue.Comment: 28 pages, 10 figures, submitted to Biophysical Journa