2,258 research outputs found
Searching for additional heating - [OII] emission in the diffuse ionized gas of NGC891, NGC4631 and NGC3079
We present spectroscopic data of ionized gas in the disk--halo regions of
three edge-on galaxies, NGC 891, NGC 4631 and NGC 3079, covering a wavelength
range from [\ion{O}{2}] 3727\AA to [\ion{S}{2}] 6716.4\AA.
The inclusion of the [\ion{O}{2}] emission provides new constraints on the
properties of the diffuse ionized gas (DIG), in particular, the origin of the
observed spatial variations in the line intensity ratios. We used three
different methods to derive electron temperatures, abundances and ionization
fractions along the slit. The increase in the [\ion{O}{2}]/H line ratio
towards the halo in all three galaxies requires an increase either in electron
temperature or in oxygen abundance. Keeping the oxygen abundance constant
yields the most reasonable results for temperature, abundances, and ionization
fractions. Since a constant oxygen abundance seems to require an increase in
temperature towards the halo, we conclude that gradients in the electron
temperature play a significant role in the observed variations in the optical
line ratios from extraplanar DIG in these three spiral galaxies.Comment: 43 pages, 29 figure
Placing the RPL32 Promoter Upstream of a Second Promoter Results in a Strongly Increased Number of Stably Transfected Mammalian Cell Lines That Display High Protein Expression Levels
The use of high stringency selection systems commonly results in a strongly diminished number of stably transfected mammalian cell lines. Here we placed twelve different promoters upstream of an adjacent primary promoter and tested whether this might result in an increased number of colonies; this is in the context of a stringent selection system. We found that only the promoter of the human ribosomal protein, RPL32, induced a high number of colonies in CHO-DG44 cells. This phenomenon was observed when the RPL32 promoter was combined with the CMV, SV40, EF1-α, and the β-actin promoters. In addition, these colonies displayed high protein expression levels. The RPL32 promoter had to be functionally intact, since the deletion of a small region upstream of the transcription start site demolished its positive action. We conclude that adding the RPL32 promoter to an expression cassette in cis may be a powerful tool to augment gene expression levels
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Chronic nicotine administration restores brain region specific upregulation of oxytocin receptor binding levels in a G72 mouse model of schizophrenia.
Nicotine dependence and schizophrenia are two mental health disorders with remarkably high comorbidity. Cigarette smoking is particularly prevalent among schizophrenic patients and it is hypothesized to comprise a form of self-medication for relieving cognitive deficits in these patients. Emerging evidence suggests a role of the neurohypophysial peptide oxytocin in the modulation of drug addiction, as well as schizophrenia symptomology; however, the underlying mechanism remains unclear. Therefore, we sought to investigate the effects of chronic nicotine administration on oxytocin receptor (OTR) binding in the brain of a transgenic mouse model of schizophrenia that carries a bacterial artificial chromosome of the human G72/G30 locus (G72Tg). Female wild-type (WT) and heterozygous G72 transgenic CD-1 mice were treated with a chronic nicotine regimen (24 mg/kg/day, osmotic minipumps for 14 days) and quantitative autoradiographic mapping of oxytocin receptors was carried out in brains of these animals. OTR binding levels were higher in the cingulate cortex (CgCx), nucleus accumbens (Acb) and central amygdala (CeA) of saline treated G72Tg mice compared with WT control mice. Chronic nicotine administration reversed this upregulation in the CgCx and CeA. Interestingly, chronic nicotine administration induced an increase in OTR binding in the CeA of solely WT mice. These results indicate that nicotine administration normalizes the dysregulated central oxytocinergic system of this mouse model of schizophrenia and may contribute towards nicotine's ability to modulate cognitive deficits which are common symptoms of schizophrenia. This article is protected by copyright. All rights reserved
Diffuse Ionized Gas in the Dwarf Irregular Galaxy DDO 53
The spectral characteristics throughout the dwarf irregular galaxy DDO 53 are
studied. The results are very similar to those for other irregular galaxies:
high excitation and low values of the [SII]/Halpha ratio. The most likely
ionization source is photon leakage from the classical HII regions, without any
other source, although the interstellar medium of the galaxy is quite
perturbed. Moreover, the physical conditions throughout the galaxy do not
change very much because both the photon leakage percentage and the ionization
temperature are very similar. In addition, the determined metal content for two
HII regions indicates that DDO 53 is a low-metallicity galaxy.Comment: 32 pages, 9 figures, 7 tables. AJ, in pres
Development of a SiPM Camera for a Schwarzschild-Couder Cherenkov Telescope for the Cherenkov Telescope Array
We present the development of a novel 11328 pixel silicon photomultiplier
(SiPM) camera for use with a ground-based Cherenkov telescope with
Schwarzschild-Couder optics as a possible medium-sized telescope for the
Cherenkov Telescope Array (CTA). The finely pixelated camera samples air-shower
images with more than twice the optical resolution of cameras that are used in
current Cherenkov telescopes. Advantages of the higher resolution will be a
better event reconstruction yielding improved background suppression and
angular resolution of the reconstructed gamma-ray events, which is crucial in
morphology studies of, for example, Galactic particle accelerators and the
search for gamma-ray halos around extragalactic sources. Packing such a large
number of pixels into an area of only half a square meter and having a fast
readout directly attached to the back of the sensors is a challenging task. For
the prototype camera development, SiPMs from Hamamatsu with through silicon via
(TSV) technology are used. We give a status report of the camera design and
highlight a number of technological advancements that made this development
possible.Comment: 8 pages, 5 figures, In Proceedings of the 34th International Cosmic
Ray Conference (ICRC2015), The Hague, The Netherlands. All CTA contributions
at arXiv:1508.0589
Signature of Fermi surface anisotropy in point contact conductance in the presence of defects
In a previous paper (Avotina et al.,Phys. Rev. B Vol.71, 115430 (2005)) we
have shown that in principle it is possible to image the defect positions below
a metal surface by means of a scanning tunnelling microscope. The principle
relies on the interference of electron waves scattered on the defects, which
give rise to small but measurable conductance fluctuations. Whereas in that
work the band structure was assumed to be free-electron like, here we
investigate the effects of Fermi surface anisotropy. We demonstrate that the
amplitude and period of the conductance oscillations are determined by the
local geometry of the Fermi surface. The signal results from those points for
which the electron velocity is directed along the vector connecting the point
contact to the defect. For a general Fermi surface geometry the position of the
maximum amplitude of the conductance oscillations is not found for the tip
directly above the defect. We have determined optimal conditions for
determination of defect positions in metals with closed and open Fermi
surfaces.Comment: 23 pages, 8 figure
Physical Conditions in Barnard's Loop, Components of the Orion-Eridanus Bubble, and Implications for the WIM Component of the ISM
We have supplemented existing spectra of Barnard's Loop with high accuracy
spectrophotometry of one new position. Cloudy photoionization models were
calculated for a variety of ionization parameters and stellar temperatures and
compared with the observations. After testing the procedure with recent
observations of M43, we establish that Barnard's Loop is photoionized by four
candidate ionizing stars, but agreement between the models and observations is
only possible if Barnard's Loop is enhanced in heavy elements by about a factor
of 1.4. Barnard's Loop is very similar in properties to the brightest
components of the Orion-Eridanus Bubble and the Warm Ionized Medium (WIM). We
are able to establish models that bound the range populated in low-ionization
color-color diagrams (I([SII])/I(H{\alpha}) versus I([NII])/I(H{\alpha})) using
only a limited range of ionization parameters and stellar temperatures.
Previously established variations in the relative abundance of heavy elements
render uncertain the most common method of determining electron temperatures
for components of the Orion-Eridanus Bubble and the WIM based on only the
I([NII])/I(H{\alpha}) ratio, although we confirm that the lowest surface
brightness components of the WIM are on average of higher electron temperature.
The electron temperatures for a few high surface brightness WIM components
determined by direct methods are comparable to those of classical bright H II
regions. In contrast, the low surface brightness HII regions studied by the
Wisconsin H{\alpha} Mapper are of lower temperatures than the classical bright
HII regions
Confirmation of the topology of the Wendelstein 7-X magnetic field to better than 1:100,000
Fusion energy research has in the past 40 years focused primarily on the tokamak concept, but recent advances in plasma theory and computational power have led to renewed interest in stellarators. The largest and most sophisticated stellarator in the world, Wendelstein 7-X (W7-X), has just started operation, with the aim to show that the earlier weaknesses of this concept have been addressed successfully, and that the intrinsic advantages of the concept persist, also at plasma parameters approaching those of a future fusion power plant. Here we show the first physics results, obtained before plasma operation: that the carefully tailored topology of nested magnetic surfaces needed for good confinement is realized, and that the measured deviations are smaller than one part in 100,000. This is a significant step forward in stellarator research, since it shows that the complicated and delicate magnetic topology can be created and verified with the required accuracy.EURATOM 633053U.S. Department of Energy DE-AC02-09CH1146
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