Synthesis, anti-microbial and anti-mutagenic activities of some Schiff bases derivatives containing thiophene group

WOS: 000493109500021The aim of this study is to investigate for the first time in vitro antimicrobial and antimutagenic activities of Schiff bases included the azomethine group. Antimutagenic activity was evaluated by micronucleus (MN) assay. These group have been examined for antibacterial activity against pathogenic strains, Staphylococcus aureus, Escherichia coli, Salmonella typhi H, Brucella abortus, Micrococcus luteus, Bacillus cereus, Pseudomonas aeroginosa and antifungal activity against Candida albicans and Saccharomyces cerevisiae. The results of MN showed that Schiff bases ( (E)-N-(4-chlorophenyl)-1-(5-nitrothiophen-2 -yl)methanimine ; (E)-N-(2,4-dichlorophenyl)-1-(5-nitrothiophen-2-yl) methanimine) different concentrations decreased the toxic effects of Aflatoxin B-1. Especially, high concentration (20 mu M) of (E)-N-(4-chlorophenyl)-1-(5-nitrothiophen-2-yl) methanimine (compound 1) has strong antimutagenic activity. In our in vitro test systems, it was observed that Schiff bases had antimutagenic effects on human lymphocytes. On the other hand these compounds were also found to possess antimicrobial activity against some test bacteria and yeast. The antimicrobial test results of these Schiff bases included the azomethine group exhibited better activity than some known antibiotics. In particular, Compound 1 were more potent bactericides than all of the substances synthesized. In conclusion, this Schiff bases included the azomethine group can be use pharmacy industries as recognized with their noncytotoxic, antimicrobial and antimutagenic features

Molecular characterization of Rhizobium strains isolated from wild chickpeas collected from high altitudes in Erzurum-Turkey

The fixation of N2 by legumes plays key role in agricultural sustainability. Moreover, the further assessment of rhizobial genetic diversity is contributing both to the worldwide knowledge of biodiversity of soil microorganisms and to the usefulness of rhizobial collections, and it is developing long-term strategies to increase contributions of legume-fixed to agricultural productivity. In the last decades, the use of molecular techniques has been contributed greatly to enhance the knowledge of rhizobial diversity. This study was conducted to determine the phenotypic and genotypic differences in Rhizobium leguminosarum subsp. ciceri strains isolated from perennial wild chickpeas (Cicer anatolicum) from high altitudes (2000-2500 m) in mountains of Erzurum, Eastern Anatolia, Turkey. In this study, rep-PCR (ERIC-, REP- and BOX-PCR) fingerprinting methods were used for the genotypic characterization and phylogenetic analysis of Rhizobium leguminosarum subsp. ciceri strains isolated from perennial wild chickpeas. The results showed a high intraspecies diversity among the strains in terms of rep-PCR (ERIC-, REP- and BOX-PCR) profiles. Copyright © 2009 Bucharest University

Synthesis of some novel isoxazolidine derivatives via 1,3-dipolar cycloaddition and their biological evaluation

A series of novel substituted isoxazolidine derivatives were synthesized by 1,3-dipolar cycloaddition reactions of a-aryl-N-methyl nitrones with diethyl maleate. The structures of the synthesized compounds were characterized using spectroscopic methods. All the synthesized compounds were screened for their antibacterial activities against Gram-positive and Gram-negative bacteria and for their antifungal activities against a yeast strain. The results show that all the synthesized compounds displayed significant activity against S. epidermidis, M. luteus, B. cereus, B. abortus and C. albicans when compared to standard drugs. © 2019, Gazi University Eti Mahallesi. All rights reserved

Synthesis of some novel isoxazolidine derivatives via 1,3-dipolar cycloaddition and their biological evaluation

A series of novel substituted isoxazolidine derivatives were synthesized by 1,3-dipolar cycloaddition reactions of a-aryl-N-methyl nitrones with diethyl maleate. The structures of the synthesized compounds were characterized using spectroscopic methods. All the synthesized compounds were screened for their antibacterial activities against Gram-positive and Gram-negative bacteria and for their antifungal activities against a yeast strain. The results show that all the synthesized compounds displayed significant activity against S. epidermidis, M. luteus, B. cereus, B. abortus and C. albicans when compared to standard drugs. © 2019, Gazi University Eti Mahallesi. All rights reserved

Synthesis and in-vitro antimicrobial and antimutagenic activities of some novel 2-(2-hydroxybenzylideneamino)-5,7-dihydro-4hthieno[ 2,3-c]pyran-3-carbonitrile derivatives

In this investigation, a series of some novel 2-(2-hydroxybenzylideneamino)-5,7-dihydro-4Hthieno[ 2,3-c]pyran-3-carbonitriles (4a-f) have been synthesized. These substances have all been examined for antibacterial activities against pathogenic strains Listeria monocytogenes 4b, Staphylococcus aureus, Escherichia coli, Salmonella typhi H, Pseudomonas putida, Brucella abortus, Shigella dysenteriae.Thype 7, Staphylococcus epidermis, Micrococcus luteus, Bacillus cereus, Enterobacter aerogenes and antifungal activity against Candida albicans. Most of the studied compounds were found effective against bacteria and yeast. Compound 4f exhibited activity against Pseudomonas putida, Brucella abortus, Staphylococcus aureus, Candida albicans and 4c Brucella abortus, Staphylococcus epidermis comparable to standard antibiotics. In addition, the anti-genotoxic activity of these substances was evaluated by a micronucleus (MN) test. Among the synthesized compounds, 4c was found to have the most anti-mutagenic effect against AFB1. It was also found that different concentrations of these substances suppressed the mutagenic effects of Aflatoxin B1 (AFB1) in the MN test (however 10 µM is the most effective dose). It seems that the antimutagenic effects of these substances may originate from their antioxidant potency or reaction of the aflatoxine lactone carbonyl functional group

The effect of cold atmospheric plasma (NO) alone and in combination with NPH insulin on the full-thickness excisional wound healing in a diabetic rat model

This study was planned to investigate an alternative treatment modality in diabetic wound healing. In this experimental study, the efficacy of both cold atmospheric plasma/nitric oxide (NO) and NPH insulin ointment, recently known to have beneficial effects on wound healing, was investigated in diabetic wound healing. Twenty-four (24) diabetic rats were divided into four groups DC, DI, DNO and DINO (diabetic control, diabetic insulin, diabetic nitric oxide, diabetic insulin + nitric oxide groups). No treatment was applied to the DC group, NPH insulin was applied to the DI group, CAP/NO was applied to the DNO group, and CAP/NO + NPH insulin was applied to the DINO group once daily for 14 days. The wound area reduction and the wound contraction rate were calculated on the basis of the tissue sections taken, and histopathological and genetic analyses were carried out. Compared to the control group, exogenous NO gas was found to be a potent antibacterial agent in the diabetic wound healing, causing a reduction in the wound area (P = 0.034), an increased contraction rate (P = 0.021), epithelialisation (P = 0.02), collagen organisation (P = 0.006) and a reduction in the number of inflammatory cells (P = 0.002). A significant increase in the expression of IL-8 mRNA was observed (P = 0.026). It was concluded that NPH insulin alone contributes to wound healing, but it is not necessary to use it together with exogenous NO gas