Significance of the Sm-Nd isotopic systematics of the Akilia Association

Samarium-Neodymium analyses were carried out on fourteen samples of basic to ultrabasic metavolcanics from several enclaves of the Amitsoq gneisses (T = to or approximately 3,700 Ma). Field observations suggest that all the analyzed rocks belong to the pre-Amitsoq Akilia Association. Consequently, a minimum age of 3,700 Ma is postulated for the emplacement of their protoliths. When all the data points are put together in a conventional isochron diagram, no clear isochron relationship can be discerned. However, the points seem to fall within a band broadly corresponding to an age of 3,600 Ma. The isotopic results are difficult to interpret satisfactorily. Two contrasting interpretations are offered and summarized: (1) data scatter as a result of open system behavior; and (2) data scatter due to a melange of data sets defining two distinct isochrons

Regional variation in the Amitsoq gneisses related to crustal levels during late Archean granulite facies metamorphism: Southern west Greenland

The dominant lithology at Kangimut sangmissoq is described as nebulitic tonalitic gneiss containing highly distended plagioclase phyric amphibolites. The gneiss amphibolite complex was intruded by Nuk gneiss between 3.05 and 2.90 Ga and later (2.6 to 2.7 Ga) by post granulite facies granitoid sheets. The amphibolites are though to be Ameralik dikes and the older gray gneiss are then Amitsoq by definition. The problem arises when the isotopic data are considered, none of which indicate rocks older that about 3.0 Ga

Significance of the late Archaean granulite facies terrain boundaries, Southern West Greenland

Three distinct episodes and occurrences of granulite metamorphism in West Greenland are described: (1) the oldest fragmentary granulites occur within the 3.6-Ga Amitsoq gneisses and appear to have formed 200 Ma after the continental crust in which they lie (Spatially associated rapakivi granites have zircon cores as old as 3.8 Ga, but Rb-Sr, whole-rock Pb-Pb, and all other systems give 3.6 Ga, so these granulites apparently represent a later metamorphic event); (2) 3.0-Ga granulites of the Nordlandet Peninsula NW of Godthaab, developed immediately after crustal formation in hot, dry conditions, are carbonate-free, associated with voluminous tonalite, and formed at peak metamorphic conditions of 800 C and 7 to 8 kbar (Synmetamorphic trondhjemite abounds and the activity of H2O has been indicated by Pilar to have varied greatly); and (3) 2.8-Ga granulites south of Godthaab, lie to the south of retrogressed amphibolite terranes. Prograde amphibolite-granulite transitions are clearly preserved only locally at the southern end of this block, near Bjornesund, south of Fiskenaesset. Progressively deeper parts of the crust are exposed from south to north as a major thrust fault is approached. Characteristic big hornblende pegmatites, which outcrop close to the thrust in the east, have been formed by replacement of orthopyroxene. Comparable features were not seen in South Indian granulites. It was concluded that no one mechanism accounts for the origin of all granulites in West Greenland. Various processes have interacted in different ways, and what happened in individual areas must be worked out by considering all possible processes

Strontium and neodymium isotopic variations in early Archean gneisses affected by middle to late Archean high-grade metamorphic processes: West Greenland and Labrador

Relicts of continental crust formed more than 3400 Ma ago are preserved fortuitously in most cratons. The cratons provide the most direct information about crust and mantle evolutionary processes during the first billion years of Earth history. In view of their polymetamorphic character, these terrains are commonly affected by subsequent tectonothermal events. Hence, their isotope systematics may be severely disturbed as a result of bulk chemical change or local isotopic homogenization. This leads to equivocal age and source information for different components within these terrains. The Sr and Nd isotopic data are presented for early Archean gneisses from the North Atlantic Craton in west Greenland and northern Labrador which were affected by younger metamorphic events

Parasite specific energy in human filariasis; insights after analysis of parasite antigen-driven lymphokine production

The antigen-specific immune unresponsiveness seen in bancroftian filariasis was studied by examining lymphokine production in peripheral blood mononuclear cells (PBMC) or PBMC subpopulations from 10 patients with asymptomatic microfilaremia, 13 patients with elephantiasis and 6 normal North Americans. In each group of patients, the kinetics of the lymphokine response and the response to mitogens and nonparasite antigens did not differ significantly. In marked contrast, when antigeninduced lymphokine production was examined, most patients with microfilaremia were unable to produce either interleukin 2 (IL- 2) or y-interferon (i.e., were nonresponders), and the few who could (hyporesponders, generally with quite low microfilaremia levels) did so at levels significantly less than those of patients with elephantiasis, all of whom showed strong responses to parasite antigen. Removal of neither adherent cells or T8+ cells affected the parasite-specific anergy seen in those with microfilaremia, suggesting a state of T cell tolerance to the parasite in patients with this most common clinical manifestation of bancroftian filariasis

Development of Onchocerca volvulus in humanized NSG mice and detection of parasite biomarkers in urine and serum.

BACKGROUND: The study of Onchocerca volvulus has been limited by its host range, with only humans and non-human primates shown to be susceptible to the full life cycle infection. Small animal models that support the development of adult parasites have not been identified. METHODOLOGY/PRINCIPAL FINDINGS: We hypothesized that highly immunodeficient NSG mice would support the survival and maturation of O. volvulus and alteration of the host microenvironment through the addition of various human cells and tissues would further enhance the level of parasite maturation. NSG mice were humanized with: (1) umbilical cord derived CD34+ stem cells, (2) fetal derived liver, thymus and CD34+ stem cells or (3) primary human skeletal muscle cells. NSG and humanized NSG mice were infected with 100 O. volvulus infective larvae (L3) for 4 to 12 weeks. When necropsies of infected animals were performed, it was observed that parasites survived and developed throughout the infection time course. In each of the different humanized mouse models, worms matured from L3 to advanced fourth stage larvae, with both male and female organ development. In addition, worms increased in length by up to 4-fold. Serum and urine, collected from humanized mice for identification of potential biomarkers of infection, allowed for the identification of 10 O. volvulus-derived proteins found specifically in either the urine or the serum of the humanized O. volvulus-infected NSG mice. CONCLUSIONS/SIGNIFICANCE: The newly identified mouse models for onchocerciasis will enable the development of O. volvulus specific biomarkers, screening for new therapeutic approaches and potentially studying the human immune response to infection with O. volvulus

Identification of circulating parasite antigen in patients with bancroftian filariasis

Because many cases of lymphatic filariasis cannot be diagnosed either clinically or by immunodiagnostic test based on antibody detection, recent efforts have been more directed towards developing methods for detecting parasite antigen in the blood or urine. Using a solid phase (Sepharose 4B) two-site immunoradiometric assay (IRMA) employing hyperimmune rabbit antifilarial antisera, we have previously shown (Hamilton et al., 1984) that essentially all cases ofpatent (ie. microfilaremic) infection in patients with bancroftian filariasis can be detected by this semi-quantitative assay as well as some individuals with amicrofilaremic (i.e., 'cryptic') infection. The present communication reports the results of studies that identify a prominent circulating antigen detected by this IRMA in sera from patients with microfilaremia. The antigen was eluted from Sepharosebound rabbit polyclonal antiserum that had been reacted with known antigen positive sera. It was run in SDS-PAGE, blotted to nitrocellulose paper and identified autoradiographically using '25l-labelled rabbit antifilarial antiserum. Its high molecular weight (- 200 kD), stability to acid and boiling, and sensitivity to pronase and periodate suggest its being a glycoprotein. Isolation of this antigen will permit the development of specific reagents (such as monoclonal antibodies) which should enhance both the sensitivity and utility of the currently available antigen detection systems

Heritable Factors Play a Major Role in Determining Host Responses to \u3ci\u3eWuchereria bancrofti\u3c/i\u3e Infection in an Isolated South Pacific Island Population

Background. It is increasingly recognized that host genetic factors may play an important role in determining the outcome of filarial infections. To test this hypothesis in bancroftian lymphatic filariasis, pedigree data were collected twice during an 18-year period from an isolated Polynesian population living on a Pacific island where lymphatic filariasis is endemic. Methods. Using variance-component analysis, we examined the contribution of shared genetic and environmental effects on host clinical and immune responses to filarial infection, along with potential confounding determinants. Results. Sex was found to have a negligible influence on heritability estimates, but shared-household effects accounted for up to 32% of host variability. After accounting for these shared-household effects, heritability estimates suggested that levels of microfilariae and circulating adult worm antigen, as well as host eosinophil and immunoglobulin G antibody responses to larval and adult worm antigens, were highly heritable (range of heritability estimates, 0.15-0.84). Conclusions. These data provide evidence of a key role for genetic factors in determining the host response to filarial infections in humans and emphasize the complexity of the relationships among the host, parasite, and environment