Physicochemical properties, cytotoxicity, and antimicrobial activity of sulphated zirconia nanoparticles

Ae Mftah,1 Fatah H Alhassan,2,3 Mothanna Sadiq Al-Qubaisi,4 Mohamed Ezzat El Zowalaty,4 Thomas J Webster,5,6 Mohammed Sh-eldin,7 Abdullah Rasedee,8 Yun Hin Taufiq-Yap,2,3 Shah Samiur Rashid1 1Department of Chemistry, Faculty of Industrial Sciences and Technology, University Malaysia Pahang, Malaysia; 2Catalysis Science and Technology Research Centre, 3Department of Chemistry, Faculty of Science, Universiti Putra Malaysia, Selangor, Malaysia; 4Institute of Bioscience, University Putra Malaysia, Serdang, Selangor, Malaysia; 5Department of Chemical Engineering, Northeastern University, Boston, MA, USA; 6Center of Excellence for Advanced Materials Research, King Abdulaziz University, Jeddah, Saudi Arabia; 7Solar Energy Research Institute, University Kebangsaan Malaysia, Selangor, 8Faculty of Veterinary Medicine, Universiti Putra Malaysia, Selangor, Malaysia Abstract: Nanoparticle sulphated zirconia with Brønsted acidic sites were prepared here by an impregnation reaction followed by calcination at 600°C for 3 hours. The characterization was completed using X-ray diffraction, thermal gravimetric analysis, Fourier transform infrared spectroscopy, Brunner-Emmett-Teller surface area measurements, scanning electron microscopy with energy dispersive X-ray spectroscopy, and transmission electron microscopy. Moreover, the anticancer and antimicrobial effects were investigated for the first time. This study showed for the first time that the exposure of cancer cells to sulphated zirconia nanoparticles (3.9–1,000 µg/mL for 24 hours) resulted in a dose-dependent inhibition of cell growth, as determined by (4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assays. Similar promising results were observed for reducing bacteria functions. In this manner, this study demonstrated that sulphated zirconia nanoparticles with Brønsted acidic sites should be further studied for a wide range of anticancer and antibacterial applications. Keywords: sulphated zirconia, nanoparticles, antimicrobial, anticance

Pseudomonas aeruginosa: arsenal of resistance mechanisms, decades of changing resistance profiles, and future antimicrobial therapies

Antimicrobial resistance is one of the most serious public health issues facing humans since the discovery of antimicrobial agents. The frequent, prolonged, and uncontrolled use of antimicrobial agents are major factors in the emergence of antimicrobial-resistant bacterial strains, including multidrug-resistant variants. Pseudomonas aeruginosa is a leading cause of nosocomial infections. The abundant data on the increased resistance to antipseudomonal agents support the need for global action. There is a paucity of new classes of antibiotics active against P. aeruginosa. Here, we discuss recent antibacterial resistance profiles and mechanisms of resistance by P. aeruginosa. We also review future potential methods for controlling antibiotic-resistant bacteria, such as phage therapy, nanotechnology and antipseudomonal vaccines