Anesthetic management of diaphragmatic hernia repair in a dog: a case report and literature review of anesthetic techniques

Summary This case report describes the anesthetic management and ventilation technique in the surgical treatment of traumatic diaphragmatic hernia in a dog. A 5-month-old 8-kg female terrier with a history of car accident was presented for femoral fracture repair. Before anesthetic induction, marked tachypnea and dyspnea were noted. Diaphragmatic hernia was diagnosed based upon radiographic and ultrasonographic findings. Exploratory laparotomy revealed diaphragmatic rupture and herniation of spleen, omentum, parts of liver lobes and stomach into the thoracic cavity. The importance of thorough physical examination and patient assessment, anesthetic management and monitoring, provision of adequate ventilation and oxygenation during surgery using standard ventilation equipment are discussed

Radiographic comparison of five different techniques for injection into the distal sesamoid bursa in cattle

Summary Numerous techniques for injection into the distal sesamoid bursa (navicular bursa) have been described, especially in equine, but there are few specific descriptions regarding this practice being done in cattle. Five different techniques were compared for injection into the distal sesamoid bursa in cattle including distal plantar approach parallel with the coronary band, proximal plantar approach, distal plantar approach parallel with the sole, abaxial approach, and distal interphalangeal joint injection. The results revealed that the numbers of needle insertion until proper placement is significantly less in the DIPJ and the DPPS techniques compared to the others (P<0.05). Also, based on the times of contrast agent injection after the correct successful needle insertion, there were significant differences between DIPJ with DPPCB, PP30 and the Ab45 techniques (P<0.05). According to the absence of direct communication between the distal sesamoid bursa and distal interphalangeal joint, the placement of the needle through distal plantar approach parallel with the sole was suggested

Comparing the healing properties of intra-articular injection of human dental pulp stem cells and cell-free-secretome on induced knee osteoarthritis in male rats

Objective: Osteoarthritis (OA) is a common and painful joint disease with multifactorial causes. Stem cells, due to their high ability to reproduce and differentiate, have created a new horizon in tissue engineering of cartilage and bone. Secretions are one of the new therapies that can be used with stem cells or separately. This study aimed to compare the healing effects of human dental pulp stem cells, cell-free secretome, and human dental pulp mesenchymal stem cells with secretome in the induced OA in male rats. Methods: Dental pulp mesenchymal stem cells were isolated and prepared from human dental pulp. The collagenase type II was injected into the knee of twenty-five male Sprague-Dawley rats, and after 10 weeks, OA was confirmed. Rats were divided into five groups (n = 5): 1) Human dental pulp stem cells plus secretome (HDP+Sec); 2) Human dental pulp stem cells (HDP); 3) Secretome (Sec); 4) Hyalgan as the positive control (Hya); 5) No treatment as the negative control (Ctrl). After 12 weeks since OA was confirmed, the healing process was examined by histopathology and radiology evaluations. Results: Histopathological evaluations, radiological assessments, and matrix indexes in three treatment groups significantly improved compared to the Ctrl and Hya groups. Surface in HDP+Sec was significantly better than the Ctrl group. In radiological evaluations, a significant decrease in OA was observed in the three treatment groups in comparison with the Ctrl groups. There was no significant difference between the treatment groups in any radiological and histopathological evaluations. HDP + Sec group slightly records better results compared to Sec or HDP treatment groups. Conclusion: It was concluded that human dental pulp stem cells and their secretome promote cartilage regeneration due to their cell protective potential as well as matrix degeneration reduction capacity. © 2023 Elsevier Lt