To the Detection of Bondline Flaws in Rubber/Metal Laminates a Comparison of the Sensitivity of Transverse and Lamb Waves

The use of adhesively-bonded structures is prevalent throughout the Navy since watertight rubber-to-metal bonds are required on many undersea systems including sonar transducers and electrical cables. In addition, adhesive bonds are used to transfer loads between rubber and metal adherends for limited structural applications. However, in each case the principal function of the adhesive bond is to provide watertight integrity for the bonded components in the hostile ocean environment where corrosion is of major concern

Rapid Cycling and Exceptional Yield in a Metal-Organic Framework Water Harvester.

Sorbent-assisted water harvesting from air represents an attractive way to address water scarcity in arid climates. Hitherto, sorbents developed for this technology have exclusively been designed to perform one water harvesting cycle (WHC) per day, but the productivities attained with this approach cannot reasonably meet the rising demand for drinking water. This work shows that a microporous aluminum-based metal-organic framework, MOF-303, can perform an adsorption-desorption cycle within minutes under a mild temperature swing, which opens the way for high-productivity water harvesting through rapid, continuous WHCs. Additionally, the favorable dynamic water sorption properties of MOF-303 allow it to outperform other commercial sorbents displaying excellent steady-state characteristics under similar experimental conditions. Finally, these findings are implemented in a new water harvester capable of generating 1.3 L kgMOF -1 day-1 in an indoor arid environment (32% relative humidity, 27 °C) and 0.7 L kgMOF -1 day-1 in the Mojave Desert (in conditions as extreme as 10% RH, 27 °C), representing an improvement by 1 order of magnitude over previously reported devices. This study demonstrates that creating sorbents capable of rapid water sorption dynamics, rather than merely focusing on high water capacities, is crucial to reach water production on a scale matching human consumption

An investigation of motor learning during side-step cutting, design of a randomised controlled trial

BACKGROUND: Of all athletic knee injuries an anterior cruciate ligament (ACL) rupture results in the longest time loss from sport. Regardless of the therapy chosen, conservative or reconstructive, athletes are often forced to reduce their level of physical activity and their involvement in sport. Moreover, a recent review reported prevalences of osteoarthritis ranging from 0% to 13% for patients with isolated ACL-deficient (ACL-D) knees and respectively 21% to 48% in patients with combined injuries. The need for ACL injury prevention is clear. The identification of risk factors and the development of prevention strategies may therefore have widespread health and economic implications. The focus of this investigation is to assess the role of implicit and explicit motor learning in optimising the performance of a side-step-cutting task. METHODS/DESIGN: A randomized controlled laboratory study will be conducted. Healthy basketball players, females and males, 18 years and older, with no previous lower extremity injuries, playing at the highest recreational level will be included. Subjects will receive a dynamic feedback intervention. Kinematic and kinetic data of the hip, knee and ankle and EMG activity of the quadriceps, hamstrings and gastrocnemius will be recorded. DISCUSSION: Female athletes have a significantly higher risk of sustaining an ACL injury than male athletes. Poor biomechanical and neuromuscular control of the lower limb is suggested to be a primary risk factor of an ACL injury mechanism in females. This randomized controlled trial has been designed to investigate whether individual feedback on task performance appears to be an effective intervention method. Results and principles found in this study will be applied to future ACL injury prevention programs, which should maybe more focus on individual injury predisposition. TRIAL REGISTRATION: Trial registration number NTR2250

Keratinocyte footprint assay discriminates antilaminin-332 pemphigoid from all other forms of pemphigoid diseases

Background Antilaminin-332 mucous membrane pemphigoid is a chronic severe pemphigoid disease characterized by autoantibodies to laminin-332. At present no commercial assay is available to demonstrate antilaminin-332 antibodies, and diagnosis relies on in-house techniques with limited sensitivities. Objectives In order to move, keratinocytes cultured in vitro secrete laminin-332 to attach to the culture dish. In that way, they leave behind a unique footprint trail of laminin-332. We aimed to develop a sensitive and specific laboratory assay to determine antilaminin-332 autoantibodies in patient serum based on binding of patient IgG to these unique footprints. Methods Normal human keratinocytes were grown on glass coverslips and incubated with patient or control serum for 1 h. The binding of IgG was then investigated by immunofluorescence. After validating the test for its ability to identify antilaminin-332 autoantibodies it was converted into a daily available test based on binding of IgG to dried coverslips that can be stored frozen. The staining patterns of sera from patients with antilaminin-332 pemphigoid were then compared with those of sera from patients with other autoimmune bullous diseases and normal human sera. Results IgG of all antilaminin-332 pemphigoid sera (n = 16) bound to laminin-332 footprints, while all normal human controls (n = 55) were negative. From the sera of patients with other diseases (n = 72) four sera tested positive. The footprint assay was also positive for sera that were negative by salt-split skin analysis, demonstrating that it is a very sensitive technique. Conclusions The keratinocyte footprint assay is a fast and specific assay to confirm or rule out the presence of antilaminin-332 autoantibodies. What's already known about this topic? Antilaminin-332 mucous membrane pemphigoid is a severe form of pemphigoid, and patients may have an increased risk of malignancies. The diagnosis of antilaminin-332 mucous membrane pemphigoid is complicated by the lack of specific commercial tests for antilaminin-332 antibodies and can be confirmed only in specialized laboratories. Keratinocytes in culture need laminin-332 for adhesion and migration and therefore deposit it on the bottom of the culture dish. What does this study add? The keratinocyte footprint assay detects antilaminin-332 autoantibodies in patient serum using the native laminin-332 produced by cultured keratinocytes. What is the translational message? The keratinocyte footprint assay is a fast and specific assay to confirm or rule out the presence of antilaminin-332 autoantibodies

Intra-and interobserver reliability of determining the femoral footprint of the torn anterior cruciate ligament on MRI scans

BACKGROUND: Re-injury rates following reconstruction of the anterior cruciate ligament (ACL) are significant; in more than 20% of patients a rupture of the graft occurs. One of the main reasons for graft failure is malposition of the femoral tunnel. The femoral origin of the torn ACL can be hard to visualize during arthroscopy, plus many individual variation in femoral origin anatomy exists, which may lead to this malpositioning. To develop a patient specific guide that may resolve this problem, a preoperative MRI is needed to identify the patient specific femoral origin of the ACL. The issue here is that there may be a difference in the reliability of identification of the femoral footprint of the ACL on MRI between different observers with different backgrounds and level of experience. The purpose of this study was to determine the intra- and interobserver reliability of identifying the femoral footprint of the torn ACL on MRI and to compare this between orthopedic surgeons, residents in orthopedic surgery and MSK radiologists.METHODS: MR images of the knee joint were collected retrospectively from 20 subjects with a confirmed rupture of the ACL. The 2D (coronal, sagittal, transversal) proton-density (PD) images were selected for the segmentation procedure to create 3D models of the femurs. The center of the femoral footprint of the ACL on 20 MRI scans, with visual feedback on 3D models (as reference) was determined twice by eight observers. The intra- and interobserver reliability of determining the center of the femoral footprint on MRI was evaluated. Intraclass correlation coefficients (ICCs) were calculated for the X, Y and Z coordinates separately and for a 3D coordinate.RESULTS: The mean 3D distance between the first and second assessment (intraobserver reliability) was 3.82 mm. The mean 3D distance between observers (interobserver reliability) was 8.67 mm. ICCs were excellent (&gt; 0.95), except for those between the assessments of the two MSK radiologists of the Y and Z coordinates (0.890 and 0.800 respectively). Orthopedic surgeons outscored the residents and radiologists in terms of intra- and interobserver agreement.CONCLUSION: Excellent intraobserver reliability was demonstrated (&lt; 4 mm). However the results of the interobserver reliability manifested remarkably less agreement between observers (&gt; 8 mm). An orthopedic background seems to increase both intra- and interobserver reliability. Preoperative planning of the femoral tunnel position in ACL reconstruction remains a surgical decision. Experienced orthopedic surgeons should be consulted when planning for patient specific instrumentation in ACL reconstruction.</p

Constraints on Cosmological Models from Hubble Space Telescope Observations of High-z Supernovae

We have coordinated Hubble Space Telescope photometry with ground-based discovery for three supernovae: two SN Ia near z~0.5 (SN 1997ce, SN 1997cj) and a third event at z=0.97 (SN 1997ck). The superb spatial resolution of HST separates each supernova from its host galaxy and leads to good precision in the light curves. The HST data combined with ground-based photometry provide good temporal coverage. We use these light curves and relations between luminosity, light curve shape, and color calibrated from low-z samples to derive relative luminosity distances which are accurate to 10% at z~0.5 and 20% at z=1. The redshift-distance relation is used to place constraints on the global mean matter density, Omega_matter, and the normalized cosmological constant, Omega_Lambda. When the HST sample is combined with the distance to SN 1995K (z=0.48), analyzed by the same precepts, it suggests that matter alone is insufficient to produce a flat Universe. Specifically, for Omega_matter+Omega_Lambda=1, Omega_matter is less than 1 with >95% confidence, and our best estimate of Omega_matter is -0.1 +/- 0.5 if Omega_Lambda=0. Although the present result is based on a very small sample whose systematics remain to be explored, it demonstrates the power of HST measurements for high redshift supernovae.Comment: Submitted to ApJ Letters, 3 figures, 1 plate, additional tabl

GRB 000418: A Hidden Jet Revealed?

We report on optical, near-infrared and centimeter radio observations of GRB000418 which allow us to follow the evolution of the afterglow from 2 to 200 days after the gamma-ray burst. In modeling these broad-band data, we find that an isotropic explosion in a constant density medium is unable to simultaneously fit both the radio and optical data. However, a jet-like outflow with an opening angle of 10-20 degress provides a good description of the data. The evidence in favor of a jet interpretation is based on the behavior of the radio light curves, since the expected jet break is masked at optical wavelengths by the light of the host galaxy. We also find evidence for extinction, presumably arising from within the host galaxy, with A(V)=0.4 mag, and host flux densities of F_R=1.1 uJy and F_K=1.7 uJy. These values supercede previous work on this burst due to the availability of a broad-band data set allowing a global fitting approach. A model in which the GRB explodes into a wind-stratified circumburst medium cannot be ruled out by these data. However, in examining a sample of other bursts (e.g. GRB990510, GRB000301C) we favor the jet interpretation for GRB000418.Comment: ApJ, submitte

Paraneoplastic pemphigus with fatal bronchiolitis obliterans

Paraneoplastic pemphigus (PNP) is a rare autoimmune bullous disease, associated with high mortality and morbidity. Accurate serological tools exist to confirm its diagnosis, providing the clinician considers this diagnosis. We present a case of a young woman with a painful stomatitis, which had been treated, prior to presentation at our clinic as erosive lichen planus. Additional serological tests led to the diagnosis paraneoplastisch pemphigus (PNP), which lead to further oncological screening. An intra-abdominal follicular dendritic cell sarcoma was found. Despite surgical removal of the sarcoma and treatment with high dose systemic corticosteroids, rituximab, and intraveneous immunoglobulins, the stomatitis persisted and the patient developed a bronchiolitis obliterans from which she died, six months after the diagnosis . This case illustrates the fulminant path that PNP can follow , and calls for further studies aimed at identifying the risk factors and optimal therapeutic regimens for bronchiolitis obliterans and PNP.</p