61 research outputs found
Thematically analysing social network content during disasters through the lens of the disaster management lifecycle
Social Networks such as Twitter are often used for disseminating and collecting information during natural disasters. The potential for its use in Disaster Management has been acknowledged. However, more nuanced understanding of the communications that take place on social networks are required to more effectively integrate this information into the processes within disaster management. The type and value of information shared should be assessed, determining the benefits and issues, with credibility and reliability as known concerns. Mapping the tweets in relation to the modelled stages of a disaster can be a useful evaluation for determining the benefits/drawbacks of using data from social networks, such as Twitter, in disaster management.A thematic analysis of tweets' content, language and tone during the UK Storms and Floods 2013/14 was conducted. Manual scripting was used to determine the official sequence of events, and classify the stages of the disaster into the phases of the Disaster Management Lifecycle, to produce a timeline. Twenty-five topics discussed on Twitter emerged, and three key types of tweets, based on the language and tone, were identified. The timeline represents the events of the disaster, according to the Met Office reports, classed into B. Faulkner's Disaster Management Lifecycle framework. Context is provided when observing the analysed tweets against the timeline. This illustrates a potential basis and benefit for mapping tweets into the Disaster Management Lifecycle phases. Comparing the number of tweets submitted in each month with the timeline, suggests users tweet more as an event heightens and persists. Furthermore, users generally express greater emotion and urgency in their tweets.This paper concludes that the thematic analysis of content on social networks, such as Twitter, can be useful in gaining additional perspectives for disaster management. It demonstrates that mapping tweets into the phases of a Disaster Management Lifecycle model can have benefits in the recovery phase, not just in the response phase, to potentially improve future policies and activities
FRIGA, A New Approach To Identify Isotopes and Hypernuclei In N-Body Transport Models
We present a new algorithm to identify fragments in computer simulations of
relativistic heavy ion collisions. It is based on the simulated annealing
technique and can be applied to n-body transport models like the Quantum
Molecular Dynamics. This new approach is able to predict isotope yields as well
as hyper-nucleus production. In order to illustrate its predicting power, we
confront this new method to experimental data, and show the sensitivity on the
parameters which govern the cluster formation
ΠΠ΅ΡΡΠΏΠ΅ΠΊΡΠΈΠ²ΠΈ ΡΡΠ²ΠΎΡΠ΅Π½Π½Ρ Π½ΠΎΠ²ΠΎΠ³ΠΎ Π»ΡΠΊΠ°ΡΡΡΠΊΠΎΠ³ΠΎ Π·Π°ΡΠΎΠ±Ρ Π΄Π»Ρ ΠΊΠΎΡΠ΅ΠΊΡΡΡ ΠΌΠ΅ΡΠ°Π±ΠΎΠ»ΡΡΠ½ΠΎΠ³ΠΎ ΡΠΈΠ½Π΄ΡΠΎΠΌΡ Π½Π° ΠΎΡΠ½ΠΎΠ²Ρ ΠΠΠ ΠΌΡΡΠ½ΠΈΡΡ Π·Π²ΠΈΡΠ°ΠΉΠ½ΠΎΡ Π»ΠΈΡΡΡ
Topicality. The metabolic syndrome is pathogenetically interrelated metabolic disorders in the condition of a sick person. A large set of factors is involved in its occurrence. Risk factors include genetic predisposition, overeating, insulin resistance, obesity, bad habits, hypodynamics, stress and unfavorable environmental conditions. First of all, defects of the lipid and carbohydrate metabolism provoke a cascade of genetic, metabolic, hormonal, nervous, inflammatory and other reactions and disorders in cells, tissues and organs, causing the metabolic syndrome and associated diseases, such as diabetes, kidney and gallstone disease, hypertension, platelet hyperaggregation, etc. Therefore, the rational use of synthetic and herbal medicines in the complex correction of these disorders can slow down the development of the metabolic syndrome.
Aim. To develop the method for obtaining a dry modified extract from bearberry, study its chemical composition, hypoglycemic and hypolipidemic activity in order to determine the prospects of its use for the correction of the metabolic syndrome.
Materials and methods. The study object was a dry extract of bearberry leaves modified with cysteine. HPLC and spectrophotometry were used to analyze the extract obtained. The hypoglycemic and hypolipidemic activity of dry extracts of bearberry was studied in rats with insulin resistance.
Results and discussion. The method for obtaining a dry modified extract from bearberry leaves was developed by adding cysteine. Phenologlycoside (arbutin), 2Β phenolic acids (gallic and ellagic), 6Β flavonoids, 8Β saponins were identified in the extract, and their quantitative content was determined. Hyperoside and catechin were dominant among flavonoids, and ursolic acid, uvaol, and lupeol prevailed among saponins. The content of the main groups of phenolic compounds was determined in the extract by spectrophotometry. The introduction of the dry extract from bearberry leaves modified with cysteine has a normalizing effect on metabolic disorders on the background of a high-fructose diet; therefore, it can be a promising agent for the correction of the metabolic syndrome.
Conclusions. As a result of the research conducted, a new dry extract from bearberry leaves modified with cysteine has been created. The phytochemical composition, hypoglycemic and hypolipidemic activities of the extract have been studied, indicating the prospects for its use to correct the metabolic syndrome.ΠΠΊΡΡΠ°Π»ΡΠ½ΠΎΡΡΡ. ΠΠ΅ΡΠ°Π±ΠΎΠ»ΠΈΡΠ΅ΡΠΊΠΈΠΉ ΡΠΈΠ½Π΄ΡΠΎΠΌ β ΡΡΠΎ ΠΏΠ°ΡΠΎΠ³Π΅Π½Π΅ΡΠΈΡΠ΅ΡΠΊΠΈ Π²Π·Π°ΠΈΠΌΠΎΡΠ²ΡΠ·Π°Π½Π½ΡΠ΅ ΠΌΠ΅ΡΠ°Π±ΠΎΠ»ΠΈΡΠ΅ΡΠΊΠΈΠ΅ Π½Π°ΡΡΡΠ΅Π½ΠΈΡ Π² ΡΠΎΡΡΠΎΡΠ½ΠΈΠΈ Π±ΠΎΠ»ΡΠ½ΠΎΠ³ΠΎ ΡΠ΅Π»ΠΎΠ²Π΅ΠΊΠ°. ΠΠ½Π°ΡΠΈΡΠ΅Π»ΡΠ½ΡΠΉ ΠΊΠΎΠΌΠΏΠ»Π΅ΠΊΡ ΡΠ°ΠΊΡΠΎΡΠΎΠ² ΡΡΠ°ΡΡΠ²ΡΠ΅Ρ Π² Π΅Π³ΠΎ Π²ΠΎΠ·Π½ΠΈΠΊΠ½ΠΎΠ²Π΅Π½ΠΈΠΈ. Π ΡΠ°ΠΊΡΠΎΡΠ°ΠΌ ΡΠΈΡΠΊΠ° ΠΎΡΠ½ΠΎΡΡΡΡΡ: Π³Π΅Π½Π΅ΡΠΈΡΠ΅ΡΠΊΠ°Ρ ΠΏΡΠ΅Π΄ΡΠ°ΡΠΏΠΎΠ»ΠΎΠΆΠ΅Π½Π½ΠΎΡΡΡ, ΠΈΠ·Π±ΡΡΠΎΡΠ½ΠΎΠ΅ ΠΏΠΈΡΠ°Π½ΠΈΠ΅, ΠΈΠ½ΡΡΠ»ΠΈΠ½ΠΎΡΠ΅Π·ΠΈΡΡΠ΅Π½ΡΠ½ΠΎΡΡΡ, ΠΎΠΆΠΈΡΠ΅Π½ΠΈΠ΅, Π²ΡΠ΅Π΄Π½ΡΠ΅ ΠΏΡΠΈΠ²ΡΡΠΊΠΈ, Π³ΠΈΠΏΠΎΠ΄ΠΈΠ½Π°ΠΌΠΈΡ, ΡΡΡΠ΅ΡΡΠΎΠ²ΡΠ΅ Π²ΠΎΠ·Π΄Π΅ΠΉΡΡΠ²ΠΈΡ ΠΈ Π½Π΅Π±Π»Π°Π³ΠΎΠΏΡΠΈΡΡΠ½ΡΠ΅ ΡΠΊΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΈΠ΅ ΡΡΠ»ΠΎΠ²ΠΈΡ ΠΎΠΊΡΡΠΆΠ°ΡΡΠ΅ΠΉ ΡΡΠ΅Π΄Ρ. Π ΠΏΠ΅ΡΠ²ΡΡ ΠΎΡΠ΅ΡΠ΅Π΄Ρ Π΄Π΅ΡΠ΅ΠΊΡΡ Π»ΠΈΠΏΠΈΠ΄Π½ΠΎΠ³ΠΎ ΠΈ ΡΠ³Π»Π΅Π²ΠΎΠ΄Π½ΠΎΠ³ΠΎ ΠΎΠ±ΠΌΠ΅Π½ΠΎΠ² ΠΏΡΠΎΠ²ΠΎΡΠΈΡΡΡΡ ΠΊΠ°ΡΠΊΠ°Π΄ Π³Π΅Π½Π΅ΡΠΈΡΠ΅ΡΠΊΠΈΡ
, ΠΌΠ΅ΡΠ°Π±ΠΎΠ»ΠΈΡΠ΅ΡΠΊΠΈΡ
, Π³ΠΎΡΠΌΠΎΠ½Π°Π»ΡΠ½ΡΡ
, Π½Π΅ΡΠ²Π½ΡΡ
, Π²ΠΎΡΠΏΠ°Π»ΠΈΡΠ΅Π»ΡΠ½ΡΡ
ΠΈ Π΄ΡΡΠ³ΠΈΡ
ΡΠ΅Π°ΠΊΡΠΈΠΉ ΠΈ Π½Π°ΡΡΡΠ΅Π½ΠΈΠΉ Π² ΠΊΠ»Π΅ΡΠΊΠ°Ρ
, ΡΠΊΠ°Π½ΡΡ
ΠΈ ΠΎΡΠ³Π°Π½Π°Ρ
, Π²ΡΠ·ΡΠ²Π°Π΅Ρ ΠΌΠ΅ΡΠ°Π±ΠΎΠ»ΠΈΡΠ΅ΡΠΊΠΈΠΉ ΡΠΈΠ½Π΄ΡΠΎΠΌ ΠΈ Π°ΡΡΠΎΡΠΈΠΈΡΠΎΠ²Π°Π½Π½ΡΠ΅ Ρ Π½ΠΈΠΌ Π·Π°Π±ΠΎΠ»Π΅Π²Π°Π½ΠΈΡ, ΡΠ°ΠΊΠΈΠ΅, ΠΊΠ°ΠΊ ΡΠ°Ρ
Π°ΡΠ½ΡΠΉ Π΄ΠΈΠ°Π±Π΅Ρ, ΠΏΠΎΡΠ΅ΡΠ½ΠΎ- ΠΈ ΠΆΠ΅Π»ΡΠ½ΠΎΠΊΠ°ΠΌΠ΅Π½Π½Π°Ρ Π±ΠΎΠ»Π΅Π·Π½ΠΈ, Π°ΡΡΠ΅ΡΠΈΠ°Π»ΡΠ½Π°Ρ Π³ΠΈΠΏΠ΅ΡΡΠ΅Π½Π·ΠΈΡ, Π³ΠΈΠΏΠ΅ΡΠ°Π³ΡΠ΅Π³Π°ΡΠΈΡ ΡΡΠΎΠΌΠ±ΠΎΡΠΈΡΠΎΠ² ΠΈ ΡΠΎΠΌΡ ΠΏΠΎΠ΄ΠΎΠ±Π½ΠΎΠ΅. Π Π°ΡΠΈΠΎΠ½Π°Π»ΡΠ½ΠΎΠ΅ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ΠΈΠ΅ ΡΠΈΠ½ΡΠ΅ΡΠΈΡΠ΅ΡΠΊΠΈΡ
ΠΈ ΡΠ°ΡΡΠΈΡΠ΅Π»ΡΠ½ΡΡ
Π»Π΅ΠΊΠ°ΡΡΡΠ²Π΅Π½Π½ΡΡ
ΡΡΠ΅Π΄ΡΡΠ² Π² ΠΊΠΎΠΌΠΏΠ»Π΅ΠΊΡΠ½ΠΎΠΉ ΠΊΠΎΡΡΠ΅ΠΊΡΠΈΠΈ ΡΡΠΈΡ
Π½Π°ΡΡΡΠ΅Π½ΠΈΠΉ ΠΏΠΎΠ·Π²ΠΎΠ»ΡΠ΅Ρ ΠΏΡΠΈΠΎΡΡΠ°Π½ΠΎΠ²ΠΈΡΡ ΡΠ°Π·Π²ΠΈΡΠΈΠ΅ ΠΌΠ΅ΡΠ°Π±ΠΎΠ»ΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ ΡΠΈΠ½Π΄ΡΠΎΠΌΠ°.
Π¦Π΅Π»Ρ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ. Π Π°Π·ΡΠ°Π±ΠΎΡΠ°ΡΡ ΡΠΏΠΎΡΠΎΠ± ΠΏΠΎΠ»ΡΡΠ΅Π½ΠΈΡ ΡΡΡ
ΠΎΠ³ΠΎ ΠΌΠΎΠ΄ΠΈΡΠΈΡΠΈΡΠΎΠ²Π°Π½Π½ΠΎΠ³ΠΎ ΡΠΊΡΡΡΠ°ΠΊΡΠ° ΡΠΎΠ»ΠΎΠΊΠ½ΡΠ½ΠΊΠΈ ΠΎΠ±ΡΠΊΠ½ΠΎΠ²Π΅Π½Π½ΠΎΠΉ Π»ΠΈΡΡΡΠ΅Π², ΠΈΠ·ΡΡΠΈΡΡ Π΅Π³ΠΎ Ρ
ΠΈΠΌΠΈΡΠ΅ΡΠΊΠΈΠΉ ΡΠΎΡΡΠ°Π², Π³ΠΈΠΏΠΎΠ³Π»ΠΈΠΊΠ΅ΠΌΠΈΡΠ΅ΡΠΊΡΡ ΠΈ Π³ΠΈΠΏΠΎΠ»ΠΈΠΏΠΈΠ΄Π΅ΠΌΠΈΡΠ΅ΡΠΊΡΡ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΡ Ρ ΡΠ΅Π»ΡΡ ΡΡΡΠ°Π½ΠΎΠ²Π»Π΅Π½ΠΈΡ ΠΏΠ΅ΡΡΠΏΠ΅ΠΊΡΠΈΠ²Ρ Π΅Π³ΠΎ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ΠΈΡ Π΄Π»Ρ ΠΊΠΎΡΡΠ΅ΠΊΡΠΈΠΈ ΠΌΠ΅ΡΠ°Π±ΠΎΠ»ΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ ΡΠΈΠ½Π΄ΡΠΎΠΌΠ°.
ΠΠ°ΡΠ΅ΡΠΈΠ°Π»Ρ ΠΈ ΠΌΠ΅ΡΠΎΠ΄Ρ. ΠΠ±ΡΠ΅ΠΊΡΠΎΠΌ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ Π±ΡΠ» ΡΡΡ
ΠΎΠΉ ΡΠΊΡΡΡΠ°ΠΊΡ ΡΠΎΠ»ΠΎΠΊΠ½ΡΠ½ΠΊΠΈ ΠΎΠ±ΡΠΊΠ½ΠΎΠ²Π΅Π½Π½ΠΎΠΉ Π»ΠΈΡΡΡΠ΅Π², ΠΌΠΎΠ΄ΠΈΡΠΈΡΠΈΡΠΎΠ²Π°Π½Π½ΡΠΉ ΡΠΈΡΡΠ΅ΠΈΠ½ΠΎΠΌ. ΠΠ»Ρ Π°Π½Π°Π»ΠΈΠ·Π° ΠΏΠΎΠ»ΡΡΠ΅Π½Π½ΠΎΠ³ΠΎ ΡΠΊΡΡΡΠ°ΠΊΡΠ° ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π»ΠΈ ΠΌΠ΅ΡΠΎΠ΄ ΠΠΠΠ₯ ΠΈ ΡΠΏΠ΅ΠΊΡΡΠΎΡΠΎΡΠΎΠΌΠ΅ΡΡΠΈΡ. ΠΠΈΠΏΠΎΠ³Π»ΠΈΠΊΠ΅ΠΌΠΈΡΠ΅ΡΠΊΡΡ ΠΈ Π³ΠΈΠΏΠΎΠ»ΠΈΠΏΠΈΠ΄Π΅ΠΌΠΈΡΠ΅ΡΠΊΡΡ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΡ ΡΡΡ
ΠΈΡ
ΡΠΊΡΡΡΠ°ΠΊΡΠΎΠ² ΡΠΎΠ»ΠΎΠΊΠ½ΡΠ½ΠΊΠΈ ΠΎΠ±ΡΠΊΠ½ΠΎΠ²Π΅Π½Π½ΠΎΠΉ Π»ΠΈΡΡΡΠ΅Π² ΠΈΠ·ΡΡΠ°Π»ΠΈ Π½Π° ΠΊΡΡΡΠ°Ρ
Ρ ΠΈΠ½ΡΡΠ»ΠΈΠ½ΠΎΡΠ΅Π·ΠΈΡΡΠ΅Π½ΡΠ½ΠΎΡΡΡΡ.
Π Π΅Π·ΡΠ»ΡΡΠ°ΡΡ ΠΈ ΠΎΠ±ΡΡΠΆΠ΄Π΅Π½ΠΈΠ΅. Π Π°Π·ΡΠ°Π±ΠΎΡΠ°Π½ ΡΠΏΠΎΡΠΎΠ± ΠΏΠΎΠ»ΡΡΠ΅Π½ΠΈΡ ΡΡΡ
ΠΎΠ³ΠΎ ΠΌΠΎΠ΄ΠΈΡΠΈΡΠΈΡΠΎΠ²Π°Π½Π½ΠΎΠ³ΠΎ ΡΠΊΡΡΡΠ°ΠΊΡΠ° Ρ Π΄ΠΎΠ±Π°Π²Π»Π΅Π½ΠΈΠ΅ΠΌ ΡΠΈΡΡΠ΅ΠΈΠ½Π° Ρ ΡΠΎΠ»ΠΎΠΊΠ½ΡΠ½ΠΊΠΈ ΠΎΠ±ΡΠΊΠ½ΠΎΠ²Π΅Π½Π½ΠΎΠΉ Π»ΠΈΡΡΡΠ΅Π². Π ΡΠΊΡΡΡΠ°ΠΊΡΠ΅ ΠΈΠ΄Π΅Π½ΡΠΈΡΠΈΡΠΈΡΠΎΠ²Π°Π½Ρ ΡΠ΅Π½ΠΎΠ»ΠΎΠ³Π»ΠΈΠΊΠΎΠ·ΠΈΠ΄ (Π°ΡΠ±ΡΡΠΈΠ½), 2Β ΡΠ΅Π½ΠΎΠ»ΠΊΠ°ΡΠ±ΠΎΠ½ΠΎΠ²ΡΠ΅ ΠΊΠΈΡΠ»ΠΎΡΡ (Π³Π°Π»Π»ΠΎΠ²Π°Ρ ΠΈ ΡΠ»Π°Π³ΠΎΠ²Π°Ρ), 6Β ΡΠ»Π°Π²ΠΎΠ½ΠΎΠΈΠ΄ΠΎΠ², 8Β ΡΠ°ΠΏΠΎΠ½ΠΈΠ½ΠΎΠ², Π° ΡΠ°ΠΊΠΆΠ΅ ΡΡΡΠ°Π½ΠΎΠ²Π»Π΅Π½ΠΎ ΠΈΡ
ΠΊΠΎΠ»ΠΈΡΠ΅ΡΡΠ²Π΅Π½Π½ΠΎΠ΅ ΡΠΎΠ΄Π΅ΡΠΆΠ°Π½ΠΈΠ΅. Π‘ΡΠ΅Π΄ΠΈ ΡΠ»Π°Π²ΠΎΠ½ΠΎΠΈΠ΄ΠΎΠ² Π΄ΠΎΠΌΠΈΠ½ΠΈΡΡΡΡΠΈΠΌΠΈ Π±ΡΠ»ΠΈ Π³ΠΈΠΏΠ΅ΡΠΎΠ·ΠΈΠ΄ ΠΈ ΠΊΠ°ΡΠ΅Ρ
ΠΈΠ½Ρ, ΡΡΠ΅Π΄ΠΈ ΡΠ°ΠΏΠΎΠ½ΠΈΠ½ΠΎΠ² β ΡΡΡΠΎΠ»ΠΎΠ²Π°Ρ ΠΊΠΈΡΠ»ΠΎΡΠ°, ΡΠ²Π°ΠΎΠ» ΠΈ Π»ΡΠΏΠ΅ΠΎΠ». Π ΡΠΊΡΡΡΠ°ΠΊΡΠ΅ ΠΌΠ΅ΡΠΎΠ΄ΠΎΠΌ ΡΠΏΠ΅ΠΊΡΡΠΎΡΠΎΡΠΎΠΌΠ΅ΡΡΠΈΠΈ ΡΡΡΠ°Π½ΠΎΠ²Π»Π΅Π½ΠΎ ΡΠΎΠ΄Π΅ΡΠΆΠ°Π½ΠΈΠ΅ ΠΎΡΠ½ΠΎΠ²Π½ΡΡ
Π³ΡΡΠΏΠΏ ΡΠ΅Π½ΠΎΠ»ΡΠ½ΡΡ
ΡΠΎΠ΅Π΄ΠΈΠ½Π΅Π½ΠΈΠΉ. ΠΠ²Π΅Π΄Π΅Π½ΠΈΠ΅ ΡΡΡ
ΠΎΠ³ΠΎ ΡΠΊΡΡΡΠ°ΠΊΡΠ° ΡΠΎΠ»ΠΎΠΊΠ½ΡΠ½ΠΊΠΈ ΠΎΠ±ΡΠΊΠ½ΠΎΠ²Π΅Π½Π½ΠΎΠΉ Π»ΠΈΡΡΡΠ΅Π², ΠΌΠΎΠ΄ΠΈΡΠΈΡΠΈΡΠΎΠ²Π°Π½Π½ΠΎΠ³ΠΎ ΡΠΈΡΡΠ΅ΠΈΠ½ΠΎΠΌ, ΠΏΡΠΎΡΠ²Π»ΡΠ΅Ρ Π½ΠΎΡΠΌΠ°Π»ΠΈΠ·ΡΡΡΠ΅Π΅ Π΄Π΅ΠΉΡΡΠ²ΠΈΠ΅ Π½Π° ΠΌΠ΅ΡΠ°Π±ΠΎΠ»ΠΈΡΠ΅ΡΠΊΠΈΠ΅ Π½Π°ΡΡΡΠ΅Π½ΠΈΡ Π½Π° ΡΠΎΠ½Π΅ Π²ΡΡΠΎΠΊΠΎΡΡΡΠΊΡΠΎΠ·Π½ΠΎΠΉ Π΄ΠΈΠ΅ΡΡ, ΠΏΠΎΡΡΠΎΠΌΡ ΠΎΠ½ ΠΌΠΎΠΆΠ΅Ρ Π±ΡΡΡ ΠΏΠ΅ΡΡΠΏΠ΅ΠΊΡΠΈΠ²Π½ΡΠΌ Π°Π³Π΅Π½ΡΠΎΠΌ Π΄Π»Ρ ΠΊΠΎΡΡΠ΅ΠΊΡΠΈΠΈ ΠΌΠ΅ΡΠ°Π±ΠΎΠ»ΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ ΡΠΈΠ½Π΄ΡΠΎΠΌΠ°.
ΠΡΠ²ΠΎΠ΄Ρ. Π ΡΠ΅Π·ΡΠ»ΡΡΠ°ΡΠ΅ ΠΏΡΠΎΠ²Π΅Π΄Π΅Π½Π½ΡΡ
ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΠΉ Π±ΡΠ» ΡΠΎΠ·Π΄Π°Π½ Π½ΠΎΠ²ΡΠΉ ΡΡΡ
ΠΎΠΉ ΡΠΊΡΡΡΠ°ΠΊΡ ΡΠΎΠ»ΠΎΠΊΠ½ΡΠ½ΠΊΠΈ ΠΎΠ±ΡΠΊΠ½ΠΎΠ²Π΅Π½Π½ΠΎΠΉ Π»ΠΈΡΡΡΠ΅Π², ΠΌΠΎΠ΄ΠΈΡΠΈΡΠΈΡΠΎΠ²Π°Π½Π½ΡΠΉ ΡΠΈΡΡΠ΅ΠΈΠ½ΠΎΠΌ, ΠΈΠ·ΡΡΠ΅Π½ Π΅Π³ΠΎ ΡΠΈΡΠΎΡ
ΠΈΠΌΠΈΡΠ΅ΡΠΊΠΈΠΉ ΡΠΎΡΡΠ°Π², Π³ΠΈΠΏΠΎΠ³Π»ΠΈΠΊΠ΅ΠΌΠΈΡΠ΅ΡΠΊΠ°Ρ ΠΈ Π³ΠΈΠΏΠΎΠ»ΠΈΠΏΠΈΠ΄Π΅ΠΌΠΈΡΠ΅ΡΠΊΠ°Ρ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΡ. Π Π΅Π·ΡΠ»ΡΡΠ°ΡΡ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΠΉ ΡΠ²ΠΈΠ΄Π΅ΡΠ΅Π»ΡΡΡΠ²ΡΡΡ ΠΎ ΠΏΠ΅ΡΡΠΏΠ΅ΠΊΡΠΈΠ²Π½ΠΎΡΡΠΈ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ΠΈΡ Π΄Π°Π½Π½ΠΎΠ³ΠΎ ΡΠΊΡΡΡΠ°ΠΊΡΠ° Π΄Π»Ρ ΠΊΠΎΡΡΠ΅ΠΊΡΠΈΠΈ ΠΌΠ΅ΡΠ°Π±ΠΎΠ»ΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ ΡΠΈΠ½Π΄ΡΠΎΠΌΠ°.ΠΠΊΡΡΠ°Π»ΡΠ½ΡΡΡΡ. ΠΠ΅ΡΠ°Π±ΠΎΠ»ΡΡΠ½ΠΈΠΉ ΡΠΈΠ½Π΄ΡΠΎΠΌ β ΡΠ΅ ΠΏΠ°ΡΠΎΠ³Π΅Π½Π΅ΡΠΈΡΠ½ΠΎ Π²Π·Π°ΡΠΌΠΎΠ·Π²βΡΠ·Π°Π½Ρ ΠΌΠ΅ΡΠ°Π±ΠΎΠ»ΡΡΠ½Ρ ΠΏΠΎΡΡΡΠ΅Π½Π½Ρ Ρ ΡΡΠ°Π½Ρ Ρ
Π²ΠΎΡΠΎΡ Π»ΡΠ΄ΠΈΠ½ΠΈ. ΠΠ΅Π»ΠΈΠΊΠΈΠΉ ΠΊΠΎΠΌΠΏΠ»Π΅ΠΊΡ ΡΠΈΠ½Π½ΠΈΠΊΡΠ² Π·ΡΠΌΠΎΠ²Π»ΡΡ ΠΉΠΎΠ³ΠΎ Π²ΠΈΠ½ΠΈΠΊΠ½Π΅Π½Π½Ρ. ΠΠΎ ΡΠ°ΠΊΡΠΎΡΡΠ² ΡΠΈΠ·ΠΈΠΊΡ Π½Π°Π»Π΅ΠΆΠ°ΡΡ: Π³Π΅Π½Π΅ΡΠΈΡΠ½Π° ΡΡ
ΠΈΠ»ΡΠ½ΡΡΡΡ, Π½Π°Π΄ΠΌΡΡΠ½Π΅ Ρ
Π°ΡΡΡΠ²Π°Π½Π½Ρ, ΡΠ½ΡΡΠ»ΡΠ½ΠΎΡΠ΅Π·ΠΈΡΡΠ΅Π½ΡΠ½ΡΡΡΡ, ΠΎΠΆΠΈΡΡΠ½Π½Ρ, ΡΠΊΡΠ΄Π»ΠΈΠ²Ρ Π·Π²ΠΈΡΠΊΠΈ, Π³ΡΠΏΠΎΠ΄ΠΈΠ½Π°ΠΌΡΡ, ΡΡΡΠ΅ΡΠΎΠ²Ρ Π²ΠΏΠ»ΠΈΠ²ΠΈ ΡΠ° Π½Π΅ΡΠΏΡΠΈΡΡΠ»ΠΈΠ²Ρ Π΅ΠΊΠΎΠ»ΠΎΠ³ΡΡΠ½Ρ ΡΠΌΠΎΠ²ΠΈ Π΄ΠΎΠ²ΠΊΡΠ»Π»Ρ. ΠΠ°ΡΠ°ΠΌΠΏΠ΅ΡΠ΅Π΄ Π΄Π΅ΡΠ΅ΠΊΡΠΈ Π»ΡΠΏΡΠ΄Π½ΠΎΠ³ΠΎ Ρ Π²ΡΠ³Π»Π΅Π²ΠΎΠ΄Π½ΠΎΠ³ΠΎ ΠΎΠ±ΠΌΡΠ½ΡΠ² ΠΏΡΠΎΠ²ΠΎΠΊΡΡΡΡ ΠΊΠ°ΡΠΊΠ°Π΄ Π³Π΅Π½Π΅ΡΠΈΡΠ½ΠΈΡ
, ΠΌΠ΅ΡΠ°Π±ΠΎΠ»ΡΡΠ½ΠΈΡ
, Π³ΠΎΡΠΌΠΎΠ½Π°Π»ΡΠ½ΠΈΡ
, Π½Π΅ΡΠ²ΠΎΠ²ΠΈΡ
, Π·Π°ΠΏΠ°Π»ΡΠ½ΠΈΡ
ΡΠ° ΡΠ½ΡΠΈΡ
ΡΠ΅Π°ΠΊΡΡΠΉ Ρ ΠΏΠΎΡΡΡΠ΅Π½Ρ Ρ ΠΊΠ»ΡΡΠΈΠ½Π°Ρ
, ΡΠΊΠ°Π½ΠΈΠ½Π°Ρ
Ρ ΠΎΡΠ³Π°Π½Π°Ρ
, ΡΠΎ ΡΠΏΡΠΈΡΠΈΠ½ΡΡ ΠΌΠ΅ΡΠ°Π±ΠΎΠ»ΡΡΠ½ΠΈΠΉ ΡΠΈΠ½Π΄ΡΠΎΠΌ Ρ Π°ΡΠΎΡΡΠΉΠΎΠ²Π°Π½Ρ Π· Π½ΠΈΠΌ Π·Π°Ρ
Π²ΠΎΡΡΠ²Π°Π½Π½Ρ, ΡΠΊ-ΠΎΡ: ΡΡΠΊΡΠΎΠ²ΠΈΠΉ Π΄ΡΠ°Π±Π΅Ρ; Π½ΠΈΡΠΊΠΎΠ²ΠΎ- ΡΠ° ΠΆΠΎΠ²ΡΠ½ΠΎΠΊΠ°ΠΌβΡΠ½Π° Ρ
Π²ΠΎΡΠΎΠ±ΠΈ; Π°ΡΡΠ΅ΡΡΠ°Π»ΡΠ½Π° Π³ΡΠΏΠ΅ΡΡΠ΅Π½Π·ΡΡ, Π³ΡΠΏΠ΅ΡΠ°Π³ΡΠ΅Π³Π°ΡΡΡ ΡΡΠΎΠΌΠ±ΠΎΡΠΈΡΡΠ² ΡΠΎΡΠΎ. Π Π°ΡΡΠΎΠ½Π°Π»ΡΠ½Π΅ Π²ΠΈΠΊΠΎΡΠΈΡΡΠ°Π½Π½Ρ ΡΠΈΠ½ΡΠ΅ΡΠΈΡΠ½ΠΈΡ
Ρ ΡΠΎΡΠ»ΠΈΠ½Π½ΠΈΡ
Π»ΡΠΊΠ°ΡΡΡΠΊΠΈΡ
Π·Π°ΡΠΎΠ±ΡΠ² Ρ ΠΊΠΎΠΌΠΏΠ»Π΅ΠΊΡΠ½ΡΠΉ ΠΊΠΎΡΠ΅ΠΊΡΡΡ ΡΠΈΡ
ΠΏΠΎΡΡΡΠ΅Π½Ρ Π΄ΠΎΠ·Π²ΠΎΠ»ΡΡ ΠΏΡΠΈΠ·ΡΠΏΠΈΠ½ΠΈΡΠΈ ΡΠΎΠ·Π²ΠΈΡΠΎΠΊ ΠΌΠ΅ΡΠ°Π±ΠΎΠ»ΡΡΠ½ΠΎΠ³ΠΎ ΡΠΈΠ½Π΄ΡΠΎΠΌΡ.
ΠΠ΅ΡΠ° Π΄ΠΎΡΠ»ΡΠ΄ΠΆΠ΅Π½Π½Ρ. Π ΠΎΠ·ΡΠΎΠ±ΠΈΡΠΈ ΡΠΏΠΎΡΡΠ± ΠΎΠ΄Π΅ΡΠΆΠ°Π½Π½Ρ ΡΡΡ
ΠΎΠ³ΠΎ ΠΌΠΎΠ΄ΠΈΡΡΠΊΠΎΠ²Π°Π½ΠΎΠ³ΠΎ Π΅ΠΊΡΡΡΠ°ΠΊΡΡ ΠΌΡΡΠ½ΠΈΡΡ Π·Π²ΠΈΡΠ°ΠΉΠ½ΠΎΡ Π»ΠΈΡΡΡ, Π²ΠΈΠ²ΡΠΈΡΠΈ ΠΉΠΎΠ³ΠΎ Ρ
ΡΠΌΡΡΠ½ΠΈΠΉ ΡΠΊΠ»Π°Π΄, Π³ΡΠΏΠΎΠ³Π»ΡΠΊΠ΅ΠΌΡΡΠ½Ρ Ρ Π³ΡΠΏΠΎΠ»ΡΠΏΡΠ΄Π΅ΠΌΡΡΠ½Ρ Π°ΠΊΡΠΈΠ²Π½ΡΡΡΡ Π· ΠΌΠ΅ΡΠΎΡ Π²ΠΈΠ·Π½Π°ΡΠ΅Π½Π½Ρ ΠΏΠ΅ΡΡΠΏΠ΅ΠΊΡΠΈΠ²ΠΈ ΠΉΠΎΠ³ΠΎ Π²ΠΈΠΊΠΎΡΠΈΡΡΠ°Π½Π½Ρ Π΄Π»Ρ ΠΊΠΎΡΠ΅ΠΊΡΡΡ ΠΌΠ΅ΡΠ°Π±ΠΎΠ»ΡΡΠ½ΠΎΠ³ΠΎ ΡΠΈΠ½Π΄ΡΠΎΠΌΡ.
ΠΠ°ΡΠ΅ΡΡΠ°Π»ΠΈ ΡΠ° ΠΌΠ΅ΡΠΎΠ΄ΠΈ. ΠΠ±βΡΠΊΡΠΎΠΌ Π΄ΠΎΡΠ»ΡΠ΄ΠΆΠ΅Π½Π½Ρ Π±ΡΠ² ΡΡΡ
ΠΈΠΉ Π΅ΠΊΡΡΡΠ°ΠΊΡ ΠΌΡΡΠ½ΠΈΡΡ Π·Π²ΠΈΡΠ°ΠΉΠ½ΠΎΡ Π»ΠΈΡΡΡ, ΠΌΠΎΠ΄ΠΈΡΡΠΊΠΎΠ²Π°Π½ΠΈΠΉ ΡΠΈΡΡΠ΅ΡΠ½ΠΎΠΌ. ΠΠ»Ρ Π°Π½Π°Π»ΡΠ·Ρ ΠΎΠ΄Π΅ΡΠΆΠ°Π½ΠΎΠ³ΠΎ Π΅ΠΊΡΡΡΠ°ΠΊΡΡ Π²ΠΈΠΊΠΎΡΠΈΡΡΠΎΠ²ΡΠ²Π°Π»ΠΈ ΠΌΠ΅ΡΠΎΠ΄ ΠΠΠ Π₯ Ρ ΡΠΏΠ΅ΠΊΡΡΠΎΡΠΎΡΠΎΠΌΠ΅ΡΡΡΡ. ΠΡΠΏΠΎΠ³Π»ΡΠΊΠ΅ΠΌΡΡΠ½Ρ ΠΉ Π³ΡΠΏΠΎΠ»ΡΠΏΡΠ΄Π΅ΠΌΡΡΠ½Ρ Π°ΠΊΡΠΈΠ²Π½ΡΡΡΡ ΡΡΡ
ΠΈΡ
Π΅ΠΊΡΡΡΠ°ΠΊΡΡΠ² ΠΌΡΡΠ½ΠΈΡΡ Π·Π²ΠΈΡΠ°ΠΉΠ½ΠΎΡ Π»ΠΈΡΡΡ Π²ΠΈΠ²ΡΠ°Π»ΠΈ Π½Π° ΡΡΡΠ°Ρ
Π· ΡΠ½ΡΡΠ»ΡΠ½ΠΎΡΠ΅Π·ΠΈΡΡΠ΅Π½ΡΠ½ΡΡΡΡ.
Π Π΅Π·ΡΠ»ΡΡΠ°ΡΠΈ ΡΠ° ΡΡ
ΠΎΠ±Π³ΠΎΠ²ΠΎΡΠ΅Π½Π½Ρ. Π ΠΎΠ·ΡΠΎΠ±Π»Π΅Π½ΠΎ ΡΠΏΠΎΡΡΠ± ΠΎΠ΄Π΅ΡΠΆΠ°Π½Π½Ρ ΡΡΡ
ΠΎΠ³ΠΎ ΠΌΠΎΠ΄ΠΈΡΡΠΊΠΎΠ²Π°Π½ΠΎΠ³ΠΎ Π΅ΠΊΡΡΡΠ°ΠΊΡΡ Π· ΠΌΡΡΠ½ΠΈΡΡ Π·Π²ΠΈΡΠ°ΠΉΠ½ΠΎΡ Π»ΠΈΡΡΡ Π· Π΄ΠΎΠ΄Π°Π²Π°Π½Π½ΡΠΌ ΡΠΈΡΡΠ΅ΡΠ½Ρ. ΠΒ Π΅ΠΊΡΡΡΠ°ΠΊΡΡ ΡΠ΄Π΅Π½ΡΠΈΡΡΠΊΠΎΠ²Π°Π½ΠΎ ΡΠ΅Π½ΠΎΠ»ΠΎΠ³Π»ΡΠΊΠΎΠ·ΠΈΠ΄ (Π°ΡΠ±ΡΡΠΈΠ½), 2Β ΡΠ΅Π½ΠΎΠ»ΠΊΠ°ΡΠ±ΠΎΠ½ΠΎΠ²Ρ ΠΊΠΈΡΠ»ΠΎΡΠΈ (Π³Π°Π»ΠΎΠ²Ρ ΡΠ° Π΅Π»Π°Π³ΠΎΠ²Ρ), 6Β ΡΠ»Π°Π²ΠΎΠ½ΠΎΡΠ΄ΡΠ², 8Β ΡΠ°ΠΏΠΎΠ½ΡΠ½ΡΠ² Ρ Π²ΠΈΠ·Π½Π°ΡΠ΅Π½ΠΎ ΡΡ
ΠΊΡΠ»ΡΠΊΡΡΠ½ΠΈΠΉ Π²ΠΌΡΡΡ. Π‘Π΅ΡΠ΅Π΄ ΡΠ»Π°Π²ΠΎΠ½ΠΎΡΠ΄ΡΠ² ΠΏΠ΅ΡΠ΅Π²Π°ΠΆΠ°Π»ΠΈ Π³ΡΠΏΠ΅ΡΠΎΠ·ΠΈΠ΄ Ρ ΠΊΠ°ΡΠ΅Ρ
ΡΠ½, ΡΠ΅ΡΠ΅Π΄ ΡΠ°ΠΏΠΎΠ½ΡΠ½ΡΠ² β ΡΡΡΠΎΠ»ΠΎΠ²Π° ΠΊΠΈΡΠ»ΠΎΡΠ°, ΡΠ²Π°ΠΎΠ» Ρ Π»ΡΠΏΠ΅ΠΎΠ». Π Π΅ΠΊΡΡΡΠ°ΠΊΡΡ ΠΌΠ΅ΡΠΎΠ΄ΠΎΠΌ ΡΠΏΠ΅ΠΊΡΡΠΎΡΠΎΡΠΎΠΌΠ΅ΡΡΡΡ Π²ΠΈΡΠ²Π»Π΅Π½ΠΎ Π²ΠΌΡΡΡ ΠΎΡΠ½ΠΎΠ²Π½ΠΈΡ
Π³ΡΡΠΏ ΡΠ΅Π½ΠΎΠ»ΡΠ½ΠΈΡ
ΡΠΏΠΎΠ»ΡΠΊ. ΠΠ²Π΅Π΄Π΅Π½Π½Ρ ΡΡΡ
ΠΎΠ³ΠΎ Π΅ΠΊΡΡΡΠ°ΠΊΡΡ ΠΌΡΡΠ½ΠΈΡΡ Π·Π²ΠΈΡΠ°ΠΉΠ½ΠΎΡ Π»ΠΈΡΡΡ, ΠΌΠΎΠ΄ΠΈΡΡΠΊΠΎΠ²Π°Π½ΠΎΠ³ΠΎ ΡΠΈΡΡΠ΅ΡΠ½ΠΎΠΌ, ΠΏΡΠΎΡΠ²Π»ΡΡ Π½ΠΎΡΠΌΠ°Π»ΡΠ·ΡΠ²Π°Π»ΡΠ½Ρ Π΄ΡΡ Π½Π° ΠΌΠ΅ΡΠ°Π±ΠΎΠ»ΡΡΠ½Ρ ΠΏΠΎΡΡΡΠ΅Π½Π½Ρ Π½Π° ΡΠ»Ρ Π²ΠΈΡΠΎΠΊΠΎΡΡΡΠΊΡΠΎΠ·Π½ΠΎΡ Π΄ΡΡΡΠΈ, Ρ ΡΠΎΠΌΡ Π²ΡΠ½ ΠΌΠΎΠΆΠ΅ Π±ΡΡΠΈ ΠΏΠ΅ΡΡΠΏΠ΅ΠΊΡΠΈΠ²Π½ΠΈΠΌ Π°Π³Π΅Π½ΡΠΎΠΌ Π΄Π»Ρ ΠΊΠΎΡΠ΅ΠΊΡΡΡ ΠΌΠ΅ΡΠ°Π±ΠΎΠ»ΡΡΠ½ΠΎΠ³ΠΎ ΡΠΈΠ½Π΄ΡΠΎΠΌΡ.
ΠΠΈΡΠ½ΠΎΠ²ΠΊΠΈ. Π£ ΡΠ΅Π·ΡΠ»ΡΡΠ°ΡΡ ΠΏΡΠΎΠ²Π΅Π΄Π΅Π½ΠΈΡ
Π΄ΠΎΡΠ»ΡΠ΄ΠΆΠ΅Π½Ρ Π±ΡΠ»ΠΎ ΡΡΠ²ΠΎΡΠ΅Π½ΠΎ Π½ΠΎΠ²ΠΈΠΉ ΡΡΡ
ΠΈΠΉ Π΅ΠΊΡΡΡΠ°ΠΊΡ ΠΌΡΡΠ½ΠΈΡΡ Π·Π²ΠΈΡΠ°ΠΉΠ½ΠΎΡ Π»ΠΈΡΡΡ, ΠΌΠΎΠ΄ΠΈΡΡΠΊΠΎΠ²Π°Π½ΠΈΠΉ ΡΠΈΡΡΠ΅ΡΠ½ΠΎΠΌ, ΠΏΡΠΎΠ²Π΅Π΄Π΅Π½ΠΎ Π΄ΠΎΡΠ»ΡΠ΄ΠΆΠ΅Π½Π½Ρ ΠΉΠΎΠ³ΠΎ ΡΡΡΠΎΡ
ΡΠΌΡΡΠ½ΠΎΠ³ΠΎ ΡΠΊΠ»Π°Π΄Ρ, Π³ΡΠΏΠΎΠ³Π»ΡΠΊΠ΅ΠΌΡΡΠ½ΠΎΡ ΡΠ° Π³ΡΠΏΠΎΠ»ΡΠΏΡΠ΄Π΅ΠΌΡΡΠ½ΠΎΡ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΡ, ΡΠΎ Π·Π°ΡΠ²ΡΠ΄ΡΠΈΠ»ΠΎ ΠΏΠ΅ΡΡΠΏΠ΅ΠΊΡΠΈΠ²Π½ΡΡΡΡ ΠΉΠΎΠ³ΠΎ Π²ΠΈΠΊΠΎΡΠΈΡΡΠ°Π½Π½Ρ Π΄Π»Ρ ΠΊΠΎΡΠ΅ΠΊΡΡΡ ΠΌΠ΅ΡΠ°Π±ΠΎΠ»ΡΡΠ½ΠΎΠ³ΠΎ ΡΠΈΠ½Π΄ΡΠΎΠΌΡ
The influence of new 1,2,3-triazolo-1,4-benzodiazepine derivatives on the muscle tone of rodents
Anxiety disorders represent one of the most prevalent categories of psychiatric illnesses, affecting individuals regardless of gender, age, or social standing. They result in substantial personal and societal costs. The pursuit of novel pharmacological approaches for treating these disorders is driven by the increasing medical necessity to enhance the effectiveness and safety profiles of anxiolytic medications. Due to the fact that benzodiazepines and their derivatives have anti-anxiety, hypnotic-sedative, antidepressant, anticonvulsant, and muscle relaxant properties, they occupy a leading place in the treatment of anxiety disorders. An essential aspect of investigating the pharmacological activity of new triazolobenziazepine derivatives is assessing their impact on rodent muscle tone and coordination of movements.
The aim of the work is to find out the influence of new 1,2,3-triazolo-1,4-benzodiazepine derivatives on the muscle tone of rodents in the βvertical gridβ test and coordination of movements using the rotarod test.
Materials and methods. The objects of the study were 5 new derivatives of 1,2,3-triazolo-1,4-benzodiazepines. Before conducting in vivo experiments, these derivatives were mixed with lactose at a ratio of 1:1000. The βvertical gridβ test and rotarod test (rotating rod test) were used to reproduce the model of motor behavior of rodents.
Results. The presence of a tendency to the manifestation of a myorelaxant effect in the βvertical gridβ test was established. The indicator of the total duration of detention at the facilities was similar and did not differ significantly in the control and experimental groups at doses of 0.50 mg/kg and 0.75 mg/kg. Derivatives MA-252, MA-253 and MA-254 at a dose of 1 mg/kg reduced the total duration of retention on the vertical grid, which indicates their mild muscle relaxant effect. In the rotating rod test, MA-253 derivative at a dose of 1 mg/kg increased the retention time on the rotarod, which demonstrates greater physical endurance of the animals of these experimental groups.
Conclusions. The studyβs findings indicated that the 1,2,3-triazolo-1,4-benzodiazepine derivatives did not exhibit an adverse impact on movement coordination. Some of these derivatives demonstrated a mild muscle relaxant effect. These results support the need for further research into their influence on spontaneous motor activity. Additionally, thereβs a necessity to determine the dosage regimen, establish an effective dose, and adapt it for human use
GAS CHROMATOGRAPHY TECHNIQUE OPTIMIZATION FOR CHECKING RESIDUAL ETHANOL IN THE PREPARATION OF HETEROLOGOUS ANTI-RABIES IMMUNOGLOBULIN
The article presents the results of determination of residual ethanol in the preparation of heterologous antirabies immunoglobulin, using portable photoionization gas chromatograph PGC-1. The advantages of the method are as follows: accuracy, sensitivity, promptness. It does not require sample preparation; the results can be processed automatically
THE INFLUENCE OF COLLOID SILVER NANO-PARTICLES UPON IMMUNOBIOLOGIC PROPERTIES OF PLAGUE MICROBE CAPSULAR ANTIGEN
This study shows that Yersinia pestis capsular antigen F1 conjugated to the CS nano-particles demonstrates apparent immunogenic effects. When injected to the test animals it was able to stimulate the production of specific antibodies under smaller antigenic load, it caused activation of the genetic apparatus in the immune system cells, and it stimulated hyperplastic processes in the lymphoid organs. Solutions of CS plus antigens appeared to be stable and caused neither general nor local responses in the test animals; they were easy to make use of, thus demonstrating their potentialities to be exploited as adjuvants in immunologic practices
EVALUATION OF VIBRIO CHOLERAE 569B INABA PROTECTIVE ANTIGENES, DERIVED ON INDUSTRIAL AND DEVELOPED BIOREACTORS AS WELL AS BY IMPROVED TECHNOLOGY
We evaluated immunochemical, physical and biochemical properties of Vibrio cholerae 569B INABA protective antigenes, derived on industrial and own-developed bioreactors as well as by technology of its concentration by tangential ultrafiltration. We detected, protease, twinase and. lysophospholipase in all samples. Also, dotimmunoanalysis showed equal concentration, of cholerogen-anatoxine and. O-antigen in all samples too. Using chromatography and. electrophoresis, we found their properties as similar. Thus, we suppose to be possible using developed bioreactor as well as technology of Vibrio cholerae 569B INABA protective antigens concentration by tangential concentration during a process of synthetic oral cholera vaccine production
OPTIMIZATION OF PRESENTATION AND CONSUMER CONTAINER OF ANTI-RABIES IMMUNOGLOBULIN OBTAINED FROM HORSE SERUM
We presented the data concerning optimization of presentation and consumer container of anti-rabies immunoglobulin obtained, from horse serum.. During the experiments, ampoules and. flasks were used for primary packaging. They were filled within 5 ml of the preparation of freeze-drying. Comparative analysis of physical, chemical and. biological properties and. molecular parameters of freeze-dried and initial forms of the immunoglobulin. was carried out. Freeze-drying was demonstrated to promote stabilization of its properties and. prevent emergence of fragments and. aggregates during anti-rabies immunoglobulin storage
ISOLATION OF GLICOPROTEID FROM THE FIXED RABIES VIRUS, STRAIN Β«MOSCOW 3253Β», AND CONSTRUCTING OF DOT-IMMUNOASSAY DIAGNOSTICUM ON ITS BASIS
Described here are the results of glicoproteid isolation from the fixed rabies virus, strain Β«Moscow 3253Β», using non-ionic detergent with subsequent chromatographic purification. The obtained antigen was demonstrated to be applicable as immunoreagent for construction of diagnosticum, by means of conjugation with colloid gold nanoparticles. The diagnosticum is meant for detection of specific antibodies in immune sera of horsesproducers, and in the preparation of anti-rabies immunoglobulin, in dot-immunoassay
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