19 research outputs found
Size-selective concentration of chondrules and other small particles in protoplanetary nebula turbulence
Size-selective concentration of particles in a weakly turbulent
protoplanetary nebula may be responsible for the initial collection of
chondrules and other constituents into primitive body precursors. This paper
presents the main elements of this process of turbulent concentration. In the
terrestrial planet region, both the characteristic size and size distribution
of chondrules are explained. "Fluffier" particles would be concentrated in
nebula regions which were at a lower gas density and/or more intensely
turbulent. The spatial distribution of concentrated particle density obeys
multifractal scaling}, suggesting a close tie to the turbulent cascade process.
This scaling behavior allows predictions of the probability distributions for
concentration in the protoplanetary nebula to be made. Large concentration
factors (>10^5) are readily obtained, implying that numerous zones of particle
density significantly exceeding the gas density could exist. If most of the
available solids were actually in chondrule sized particles, the ensuing
particle mass density would become so large that the feedback effects on gas
turbulence due to mass loading could no longer be neglected. This paper
describes the process, presenting its basic elements and some implications,
without including the effects of mass loading.Comment: 34 pages, 7 figures; in press for Astrophys. J; expected Jan 01 2001
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Activation of adherent vascular neutrophils in the lung during acute endotoxemia
BACKGROUND: Neutrophils constitute the first line of defense against invading microorganisms. Whereas these cells readily undergo apoptosis under homeostatic conditions, their survival is prolonged during inflammatory reactions and they become biochemically and functionally activated. In the present study, we analyzed the effects of acute endotoxemia on the response of a unique subpopulation of neutrophils tightly adhered to the lung vasculature. METHODS: Rats were treated with 5 mg/kg lipopolysaccharide (i.v.) to induce acute endotoxemia. Adherent neutrophils were isolated from the lung vasculature by collagenase digestion and sequential filtering. Agarose gel electrophoresis, RT-PCR, western blotting and electrophoretic mobility shift assays were used to evaluate neutrophil activity. RESULTS: Adherent vascular neutrophils isolated from endotoxemic animals exhibited decreased apoptosis when compared to cells from control animals. This was associated with a marked increase in expression of the anti-apoptotic protein, Mcl-1. Cells isolated 0.5–2 hours after endotoxin administration were more chemotactic than cells from control animals and expressed increased tumor necrosis factor-alpha and cyclooxygenase-2 mRNA and protein, demonstrating that they are functionally activated. Endotoxin treatment of the animals also induced p38 and p44/42 mitogen activated protein kinases in the adherent lung neutrophils, as well as nuclear binding activity of the transcription factors, NF-κB and cAMP response element binding protein. CONCLUSION: These data demonstrate that adherent vascular lung neutrophils are highly responsive to endotoxin and that pathways regulating apoptosis and cellular activation are upregulated in these cells
Sheep in a suspension system.
<p>The suspension system was loosely wrapped around the rump and prevented the animals from lying down. The system ensured full load bearing while the animals stood and allowed free movement (A), resting (B) and sleeping (C).</p
Action units of the Sheep Grimace Scale (SGS).
<p>Action units of the Sheep Grimace Scale (SGS).</p
Determination of interrater reliability and accuracy of the SGS.
<p>(A) Plotted is the averaged detection data of all scorers (overall) vs data of the single scorer 1–6. Analysis of the interrater reliability revealed an ICC of 0.92. (B) For the determination of the accuracy, 66 pictures were analysed. 33 pictures arising from postoperative sheep and 33 from untreated sheep before surgery were assessed by dichotomous judgement. True positives: pain pictures scored as pain; true negatives: no pain pictures scored as no pain; false negatives: pain pictures scored as no pain; false positives: no pain pictures scored as pain. ICC: intraclass correlation coefficient.</p