88 research outputs found

    A tool for measuring therapeutic jurisprudence values during empirical research

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    Since its inception in the 1980s, the growing body of Therapeutic Jurisprudence scholarship has continued to challenge and optimise not only our understanding of what the law "is", but also how we can leverage its agency to improve the ways in which we apply, observe and evaluate the law. By focusing on the emotional, human and psychological consequences of legal processes, Therapeutic Jurisprudence empowers practitioners to design emotionally intelligent and remedial strategies to either minimise harmful consequences or enhance restorative legal goals and outcomes. As the influence of Therapeutic Jurisprudence scholarship and its practical applications has continued to gain traction in rapid and organic growth, collaborations brokered with a wide range of social science disciplines have called for a more robust focus on validated measurement scales. To that end, there is a clear and growing need for a suite of Therapeutic Jurisprudence specific tools for empirical evaluation. As there has been no formal attempt within the Therapeutic Jurisprudence research community to begin this process, this paper breaks new ground by providing a validated tool for empirical measurement of the therapeutic quality of judicial officers' behavioural and interactional styles in problem-solving court jurisdictions. Using original empirical data recently collected at a problem-solving court in England, the paper takes the reader through the journey of statistically validating the levied scaling systems by performing Principal Component Analysis and Cronbach's Alpha. In doing so, the paper offers an original contribution to Therapeutic Jurisprudence methodology

    Učinak parametara asimetričnog valjanja platine na promjene ukupnog pritiska valjka

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    This work shows the results of theoretical analysis of asymmetric rolling process of plates in the finishing mill of plate rolling. Its aim was to determine the influence of asymmetry velocity of working rolls on decrease of unit pressure of metal on the rolls. The lower value of the unit pressure will reduce the elastic deflection of the finishing stand and improve the cross-section shape of plate. Three-dimensional simulation of asymmetric hot rolling of S355J2G3 steel plates was done with the aid of FORGE 2008® software. The tensor polynomial interpolation was used for comparing the values of the unit pressure obtaining from symmetric and asymmetric rolling.Rad daje rezultate teorijske analize asimetričnog valjanja platine u završnom stanu. Cilj je odrediti utjecaj asimetrične brzine radnih valjaka na umanjenje jediničnog pritiska metala na valjke. Niža vrijednost jediničnog pritiska smanjiti će elastičnu deformaciju završnog stana. i poboljšati oblik presjeka platine. Trodimenzionalna simulacija asimetričnog vrućeg valjanja S355J2G3 čeličnih platina izvedena je pomoću FORGE 2008® softvera. Polinomni tenzor interpolacije rabljen je za usporedbu vrijednosti jediničnog pritiska dobijenog sa simetričnim I nesimetričnim valjanjem

    Pseudomonas aeruginosa partitioning protein ParB acts as a nucleoid-associated protein binding to multiple copies of a parS-related motif

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    ParA and ParB homologs are involved in accurate chromosome segregation in bacteria. ParBs participate in the separation of ori domains by binding to parS palindromes, mainly localized close to oriC. In Pseudomonas aeruginosa neither ParB deficiency nor modification of all 10 parSs is lethal. However, such mutants show not only defects in chromosome segregation but also growth retardation and motility dysfunctions. Moreover, a lack of parB alters expression of over 1000 genes, suggesting that ParB could interact with the chromosome outside its canonical parS targets. Here, we show that indeed ParB binds specifically to hundreds of sites in the genome. ChIP-seq analysis revealed 420 ParB-associated regions in wild-type strain and around 1000 in a ParB-overproducing strain and in various parS mutants. The vast majority of the ParB-enriched loci contained a heptanucleotide motif corresponding to one arm of the parS palindrome. All previously postulated parSs, except parS5, interacted with ParB in vivo. Whereas the ParB binding to the four parS sites closest to oriC, parS1-4, is involved in chromosome segregation, its genome-wide interactions with hundreds of parS half-sites could affect chromosome topology, compaction and gene expression, thus allowing P. aeruginosa ParB to be classified as a nucleoid-associated protein

    Retained EU Law and Implications for Pregnant Workers: A Therapeutic Jurisprudence Perspective

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    Using the lenses and language of therapeutic jurisprudence, this paper will argue that the rights of pregnant workers are vulnerable in a post-Brexit climate. Whilst the sunset clause from the Retained EU Law Bill, which would have caused all retained EU law to automatically expire at the end of 2023 unless expressly stated otherwise by Ministers, was lifted, the original drafts of the Bill made clear the government’s lack of respect for and interest in protecting workers’ rights (amongst others). Furthermore, despite the abandonment of the sunset, the now legislated Retained EU Law (Revocation and Reform) Act 2023, aiming to deal with all laws that were once of European origin, still gives Ministers wide powers with limited input from Parliament to change EU derived legislation and replace with UK provision. Using an example from employment law, specifically, pregnant workers, this paper will show that the Act is a therapeutic jurisprudence unfriendly bottle as it has the potential to violate positive physical, social, and psychological outcomes. Recognising that these laws are currently vulnerable, we urge the government to keep intact (and potentially enhance) the laws protecting pregnant workers

    Rules and Exceptions: The Role of Chromosomal ParB in DNA Segregation and Other Cellular Processes

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    Abstract: The segregation of newly replicated chromosomes in bacterial cells is a highly coordinated spatiotemporal process. In the majority of bacterial species, a tripartite ParAB-parS system, composed of an ATPase (ParA), a DNA-binding protein (ParB), and its target(s) parS sequence(s), facilitates the initial steps of chromosome partitioning. ParB nucleates around parS(s) located in the vicinity of newly replicated oriCs to form large nucleoprotein complexes, which are subsequently relocated by ParA to distal cellular compartments. In this review, we describe the role of ParB in various processes within bacterial cells, pointing out interspecies differences. We outline recent progress in understanding the ParB nucleoprotein complex formation and its role in DNA segregation, including ori positioning and anchoring, DNA condensation, and loading of the structural maintenance of chromosome (SMC) proteins. The auxiliary roles of ParBs in the control of chromosome replication initiation and cell division, as well as the regulation of gene expression, are discussed. Moreover, we catalog ParB interacting proteins. Overall, this work highlights how different bacterial species adapt the DNA partitioning ParAB-parS system to meet their specific requirements

    Increased ParB level affects expression of stress response, adaptation and virulence operons and potentiates repression of promoters adjacent to the high affinity binding sites parS3 and parS4 in Pseudomonas aeruginosa

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    Similarly to its homologs in other bacteria, Pseudomonas aeruginosa partitioning protein ParB facilitates segregation of newly replicated chromosomes. Lack of ParB is not lethal but results in increased frequency of anucleate cells production, longer division time, cell elongation, altered colony morphology and defective swarming and swimming motility. Unlike in other bacteria, inactivation of parB leads to major changes of the transcriptome, suggesting that, directly or indirectly, ParB plays a role in regulation of gene expression in this organism. ParB overproduction affects growth rate, cell division and motility in a similar way as ParB deficiency. To identify primary ParB targets, here we analysed the impact of a slight increase in ParB level on P. aeruginosa transcriptome. ParB excess, which does not cause changes in growth rate and chromosome segregation, significantly alters the expression of 176 loci. Most notably, the mRNA level of genes adjacent to high affinity ParB binding sites parS1-4 close to oriC is reduced. Conversely, in cells lacking either parB or functional parS sequences the orfs adjacent to parS3 and parS4 are upregulated, indicating that direct ParB- parS3/ parS4 interactions repress the transcription in this region. In addition, increased ParB level brings about repression or activation of numerous genes including several transcriptional regulators involved in SOS response, virulence and adaptation. Overall, our data support the role of partitioning protein ParB as a transcriptional regulator in Pseudomonas aeruginosa

    Recovery and Restorative Justice: Systems for Generating Social Justice

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    Theories underpinning restorative justice (RJ) overlap significantly with work around addiction recovery, in particular, the concept of “recovery capital”. Whilst both movements are relatively new, RJ has a longer-standing history and a greater application in practice settings, yet both are inherently 'relational theories' and should be evaluated as such. There is great value in a comparative review of the concepts, assessing how the theories and practices align, as well as how each framework may inform the other. Granfield & Cloud (2001) have shown that recovery is enhanced by establishing previously non-existent social networks through building “social capital” to access support networks and resources in the community. Similarly, Zehr & Gohar (2002:23) posit that the third pillar of any legitimate RJ practice is a social process inclusive of dialogue and engagement with those who have a “legitimate interest or stake in the offense and its resolution”. Elsewhere, Best et al. (2015) have argued that a key component of recovery theory is “community capital” and the importance of community attitudes/resources as a predictor of recovery longevity, a model that draws on Braithwaite's reintegrative shaming theory from the RJ discourse (Braithwaite, 1989). This chapter aims to advance thinking and the Volume’s objectives of challenging the RJ field by suggesting that the common implication is that the effective implementation of these principles may facilitate the generation of a virtuous cycle enhancing the wellbeing of disputants and their communities and providing sustainable pathways to effective reintegration by building capital in communities and creating a 'therapeutic landscape' for restoration and rehabilitation
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