Intensified virus production in suspension HEK293SF cells using high inoculum fed-batch or low-perfusion rate cultures with continuous harvest

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UHPFRC tensile creep at early age

Ultra high performance fibre reinforced concrete (UHPFRC) early age viscoelastic behaviour under tension was investigated. The tests results showed a high creep potential due to the high volume paste (88%). This result is of major importance because the viscoelastic properties contribute to mitigating the high early age stresses generated under restrained shrinkage. This beneficial effect was reflected by the increased linear-relationship between tensile creep and shrinkage. As expected, UHPFRC tensile creep behaviour was also sensitive to the loading level. Above 35% of the tensile strength at the loading age, the material exhibited viscoplastic behaviour. A Maxwell chain model was applied to predict the early age UHPFRC tensile creep and confirms the induced non-linear respons

Intensification of influenza virus production in fed-batch and perfusion cultures of HEK293SF cells

More than half a million people die every year from complications of seasonal influenza, and vaccination stands as the most effective method to prevent and limit outbreaks of the disease. Constant vaccine development based on emerging strains and worldwide distribution of vaccines is a great challenge for public health and vaccine manufacturers, particularly in a potential pandemic scenario. The limited flexibility of the current egg-based production system combined with recent advances in large-scale cell culture techniques have encouraged the development of cell culture processes for influenza vaccine production. While cell culture offers a valuable alternative, productivities are still low when compared to traditional egg-based systems, requiring extensive efforts in process intensification and suspension cell line development. Please click Download on the upper right corner to see the full abstract