The asparagus genome sheds light on the origin and evolution of a young Y chromosome

Several models have been proposed to explain the emergence of sex chromosomes. Here, through comparative genomics and mutant analysis, Harkess et al. show that linked but separate genes on the Y chromosome are responsible for sex determination in Asparagus, supporting a two-gene model for sex chromosome evolution

Celf1 CLIP assay on mouse lens (Fig 3G Fig 4K Fig 5C)

Data for cross-linked immunoprecipitation (CLIP) using Celf1 antibody on wild-type mouse lens followed by RT-qPCR for specific mRNAs: Dnase2b, p27Kip1, Sptb (Fig 3G, Fig 4K, Fig 5C in Siddam et al. 2018 PLOS Genetics

RT-qPCR of Dnase2b in mouse lens (Fig 3E)

RT-qPCR assay data for Dnase2b mRNA in WT and Celf1 cKO mouse lens (Fig 3E in Siddam et al. 2018 PLOS Genetics

Quantification of lamin A/C immunofluorescence in Celf1 cKO mouse lens (S11 Fig)

Data for quantification of immunofluorescence signal of lamin A/C in control and Celf1 cKO mouse lens (Supplementary Fig. S11 in Siddam et al. 2018 PLOS Genetics

Quantification of Celf1 mRNA in Celf1 cKO mouse lens (S3 Fig)

Data for RT-qPCR quantification of Celf1 mRNA in Celf1 cKO mouse lens compared to control lens (Supplementary Fig. S3 in Siddam et al. 2018 PLOS Genetics

Quantification of ocular defects in Xenopus celf1-knockdown embryos (S5 Fig)

Data for quantification of ocular phenotypes in celf1-knockdown Xenopus laevis embryos subjected to morpholino-injection on one side (Supplementary Fig. S5 in Siddam et al. 2018 PLOS Genetics