The tetraspanin CD9 controls migration and proliferation of parietal epithelial cells and glomerular disease progression

International audienc

Gratitude and leave-taking: Editorial reflections, 2003-2017

In this article we reflect on our time as editors of Psychoanalysis, Culture & Society. The article reviews some of the journal’s major contributions to psychoanalytic understanding of social and political problems; considers whether or not we are entering a post-neoliberal world; and discusses some of the challenges faced by PCS given the marginal status of psychoanalysis in the wider culture, the journal's emphasis on interdisciplinarity, and its commitment to providing a space for multiple psychoanalytic voices. The article’s later sections consider some of the areas that remain underdeveloped in the journal’s coverage. In particular, they explore the challenging task of specifying whether or not social entities, as opposed to individuals, can be said to have properties that are unconscious in the psychoanalytic sense

The generation of kidney organoids by differentiation of human pluripotent cells to ureteric bud progenitor-like cells

International audienceThis protocol presents recently developed methodologies for the differentiation of human pluripotent stem cells (hPSCs) into ureteric bud (UB) progenitor-like cells. Differentiation of human PSCs to UB progenitor-like cells allows for the generation of chimeric kidney cultures in which the human cells can self-assemble into chimeric 3D structures in combination with embryonic mouse kidney cells over a period of 18 d. UB progenitor-like cells are generated by a two-step process that combines in vitro commitment of human PSCs, whether embryonic stem cells (ESCs) or induced PSCs (iPSCs), under chemically defined culture conditions, with ex vivo cultures for the induction of 3D organogenesis. The models described here provide new opportunities for investigating human kidney development, modeling disease, evaluating regenerative medicine strategies, as well as for toxicology studies