Uticaj odabranih monoterpena na aktivnost dijagnostički značajnijih enzima u kontrolnom humanom serumu in vitro

In order to determine the terpenes effect on enzyme activity is been studied the impact of aqueous (prepared manually (I1) or ultrasound (I2) mixing) and DMSO (dimethyl sulfoxide) terpenes solution (carvacrol, linalool, nerol and geraniol) on the activity of creatine kinase, lactate dehydrogenase, alkaline phosphatase, gamma-glutamyl transferase, aspartate aminotransferase and alanine aminotransferase,as well as the impact of the same type of terpenes solution (carvacrol, linalool, nerol, geraniol, β-pinene, myrcene, citral, eucalyptol, terpineol, and eugenol) on the activity of amylase in the control sera (with the normal (N) and increased (H) activity of the ingredients. Measurement of activity was conducted after incubation of the serum sample from the 10 minutes and aqueous solutions of terpenes prepared as I1 or as I2, or solutions of terpenes in DMSO at four concentrations. All the terpenes solutions have demonstrated that the inhibitory effect depends on various parameters: kinds of serum (higher percentage of inhibition in N than the H serum), the type of solvent ( the aqueous solution as I2 and DMSO solutions showed approximately the same percentage of inhibition in both types of serum) and way of preparing the aqueous solutions (a higher percentage of inhibition have the aqueous solution of terpenes as I2 in N serum and as I1 in H serum), as well as on the type of terpenes or enzymes. Statistical data processing all terpenes solution (with the partial exception of neroli and geraniol) confirmed the grouping or by type of serum or by type of solvent or the method of preparation of aqueous solution

Experimental demonstration of a technique to generate arbitrary quantum superposition states

Using a single, harmonically trapped $^9$Be$^+$ ion, we experimentally demonstrate a technique for generation of arbitrary states of a two-level particle confined by a harmonic potential. Rather than engineering a single Hamiltonian that evolves the system to a desired final sate, we implement a technique that applies a sequence of simple operations to synthesize the state

The effect of aminoguanidine, an inducible nitric oxide synthase inhibitor, on AlCl3 toxicity in the rat hippocampus

The presented experiment was carried out to determine the effectiveness of the inducible nitric oxide synthase inhibitor - aminoguanidine in modulating the toxicity of aluminum chloride on the nitrite levels, malondialdehyde concentration, reduced glutathione content, as well as cytochrome c oxidase activity of Wistar rats. The animals were killed 3 h and 30 days after treatment and the hippocampus was removed. The biochemical results show that aluminum acts as a pro-oxidant, while aminoguanidine exerts an antioxidant action in aluminum chloride-treated animals. We have also applied immunohistochemical techniques to identify iNOS expression after the treatment. Our data suggest that aminoguanidine can be effective in the protection of toxicity induced by aluminum chloride.U eksperimentu je određivana efikasnost inhibitora inducibilne forme azot oksid sintaze - aminogvanidina, u modulaciji toksičnosti aluminijum hlorida na nivo nitrita, koncentraciju malondialdehida, sadržaj redukovanog glutationa, kao i aktivnost citohrom c oksidaze kod Wistar pacova. Životinje su dekapitovane 3 časa i 30 dana nakon odgovarajućeg tretmana i izolovan je hipokampus. Rezultati dobijeni na biohemijskom nivou pokazuju da aluminijum deluje kao pro-oksidant, dok aminogvanidin pokazuje antioksidativno dejstvo kod životinja tretiranih aluminijum hloridom. Pored toga, korišćene su i imunohistohemijske tehnike za identifikaciju iNOS ekspresije, 3 časa nakon primene odgovarajućeg tretmana. Naši rezultati pokazuju da aminogvanidin može sprečiti toksičnost indukovanu aluminijum hloridom.nul

Inducing LIPSS by multi-pass and cross-directional scanning of femtosecond beam over surface of thin metal films

During interaction of femtosecond laser beam with metal surfaces, laser induced pe- riodic nanostructures, LIPSS can be formed, which may improve properties of materials. Having excellent mechanical properties, multilayer thin films, like 5x(Al/Ti)@Si, are con- venient for forming of high quality LIPSS [1] due to their multilayer structure. We have exposed the multilayer thin film metal systems 5x(Al/Ti)@Si with femtosecond beam from the laser system Coherent Mira 900 in NIR with various scanning configurations [2]. The irradiated samples have been analyzed by Tescan Mira3 SEM. The beam scanned over the surface of the samples with multi-pass and cross-directional scanning configurations with the change of polarization direction. The formation of LIPSS is most probably due to the occurence of surface plasmon polariton, which leads to the periodic distribution of energy on the sample surface. The orientation of the LIPSS is related to the direction of the beam polarization. During multi-pass scanning, LIPSS maintained its configuration. The preservation of structures occured to some extent. Depending on the accumulated energy, two forms of LIPSS were generated: “hills”, for less accumulation, and “trenches” for greater accumulation. “Hills” are non-ablative, probably are due to the build-up of the material and are parallel to the polarization direction. “Trenches” are formed by ablation and are perpendicular to the polarization direction. During cross-directional scanning, LIPSS of orthogonal directions have been generated. The value of the “hills” period was around 360 nm and the width was ∼285 nm. The values of “trenches” period fluctuated between 320 and 380 nm, while width was between 85 and 45 nm. Proposed mechanism is that, for less accumulated energy, “hills” formed, while more accumulated energy leads to the ablation and formation of “trenches”.UltrafastLight-2018 : International Conference on Ultrafast Optical Science : Book of Abstracts, October 1-5, 2018, Moscow, Russi

Trapped-Ion Quantum Simulator: Experimental Application to Nonlinear Interferometers

We show how an experimentally realized set of operations on a single trapped ion is sufficient to simulate a wide class of Hamiltonians of a spin-1/2 particle in an external potential. This system is also able to simulate other physical dynamics. As a demonstration, we simulate the action of an $n$-th order nonlinear optical beamsplitter. Two of these beamsplitters can be used to construct an interferometer sensitive to phase shifts in one of the interferometer beam paths. The sensitivity in determining these phase shifts increases linearly with $n$, and the simulation demonstrates that the use of nonlinear beamsplitters ($n$=2,3) enhances this sensitivity compared to the standard quantum limit imposed by a linear beamsplitter ($n$=1)

Collisional kinetics of non-uniform electric field, low-pressure, direct-current discharges in H2_{2}

A model of the collisional kinetics of energetic hydrogen atoms, molecules, and ions in pure H$_2$ discharges is used to predict H$_\alpha$ emission profiles and spatial distributions of emission from the cathode regions of low-pressure, weakly-ionized discharges for comparison with a wide variety of experiments. Positive and negative ion energy distributions are also predicted. The model developed for spatially uniform electric fields and current densities less than $10^{-3}$ A/m$^2$ is extended to non-uniform electric fields, current densities of $10^{3}$ A/m$^2$, and electric field to gas density ratios $E/N = 1.3$ MTd at 0.002 to 5 Torr pressure. (1 Td = $10^{-21}$ V m$^2$ and 1 Torr = 133 Pa) The observed far-wing Doppler broadening and spatial distribution of the H$_\alpha$ emission is consistent with reactions among H$^+$, H$_2^+$, H$_3^+$, and H$^-H$ ions, fast H atoms, and fast H$_2$ molecules, and with reflection, excitation, and attachment to fast H atoms at surfaces. The H$_\alpha$ excitation and H$^-$ formation occur principally by collisions of fast H, fast H$_2$, and H$^+$ with H$_2$. Simplifications include using a one-dimensional geometry, a multi-beam transport model, and the average cathode-fall electric field. The H$_\alpha$ emission is linear with current density over eight orders of magnitude. The calculated ion energy distributions agree satisfactorily with experiment for H$_2^+$ and H$_3^+$, but are only in qualitative agreement for H$^+$ and H$^-$. The experiments successfully modeled range from short-gap, parallel-plane glow discharges to beam-like, electrostatic-confinement discharges.Comment: Submitted to Plasmas Sources Science and Technology 8/18/201