A NOVEL RP-HPLC METHOD DEVELOPMENT AND VALIDATION FOR THE QUANTIFICATION OF A POTENTIAL ANTI-DIABETIC DRUG METFORMIN HYDROCHLORIDE IN TABLET DOSAGE FORM

Objective: This study was conducted to develop a simple, economical, linear, rapid method for the assay studies of Metformin HCl by RP-HPLC method and to carry out the method validation. Methods: A simple, robust and accurate method to carryout assay of Metformin hydrochloride tablet(500 mg) by RP-HPLC method in which the stationary phase used is Shimadzu shim-pack GIST C18 column with specification (5μm×4.6×250 mm). This method involves isocratic elution of mobile phase containing 70% buffer and 30% acetonitrile. The buffer used for analysis is Tetra-Butyl Ammonium Hydroxide (0.002%), the flow rate was maintained at 0.5 ml/min. detection was done at 232 nm. Principal peak for Metformin was observed at 3.5 min and the runtime for each injection was set to 10 min. The standard solutions of Metformin were prepared using purified water (milli-Q water) and scanned from 190 nm to 400 nm. Sharp peaks were observed in the range of 232 nm and thus, wavelength of 232 nm was selected and used throughout the validation process. Results: Linearity graph generated was found acceptable and accurate and the graph was generated in the range of 50% to 150% concentration. The regression coefficient was found to be 0.999(acceptable range). Validation was carried out according to ICH guidelines and found to be acceptable. Conclusion: This developed method was found to be simple, robust, economical, accurate, linear and can be used in the assay of Metformin tablet using RP-HPLC

Chromosome Studies and Karyotype Analysis of some Triploid Banana (Musa Species) Cultivars of AAA Genomic Group

Bananas are the highly evolved, oldest fruits known to mankind. The Cavendish group cultivars are popular commercial varieties. AAA genomic group cultivars are said to have evolved from the wild AA Musa acuminata species by natural hybridization and polyploidization and these vigorous triploids were selected by man for cultivation. Basic cytological studies on banana are comparatively few due to the plant's complex nature. In this report, karyo-morphological studies on five AAA Cavendish group cultivars i.e. Robusta, Dwarf Cavendish, Grand Naine, Gros Michel and Red banana are reported. All the five cultivars had similar karyotype, except cv. Robusta. Total chromosome length was highest in Red banana and lowest in cv. Gros Michel

Dehydroxylation of Minerals of Gadisunkapur Area with Special Reference to FTIR and Thermal Study

This paper attempts to explain the presence of OH group for micas along with some sulfate, chlorite, quartz and feldspar minerals. These minerals are found to be associated with barite vein, metasediments (quartz-chlorite schist), metarhyolite and varieties of granitic rocks of Gadisunkapur area of Hungund-Kushtagi Schist belt. The samples were collected in and around Gadisunkapur village of Bagalkote district, Karnataka and further processed to determine OH group anion in different minerals present based on FTIR and Thermal studies such as Thermogravimetric analysis (TGA) and Differential Thermal analysis (DTA). The high temperature dehydroxylation is investigated using this analysis. Furthermore, present study indicates the presence of different extra species (CO2, CO32- and OH-). The results show the incorporation of CO2 into the structures due to heating or enhanced by the dehydroxylation process

A retrospective study of ectopic pregnancy

Background: Ectopic pregnancy is one of the common acute abdominal emergencies posing a serious threat to life. The overall incidence of ectopic pregnancy is on a steady increase over the last two decades. Yet the case fatality rate has come down due to early diagnosis and management. 95% of ectopic pregnancies occur in the fallopian tube, and rest 5% cumulatively in the ovary, cervix, peritoneal cavity and previous caesarean section scar. The present study was done to analyse the associated risk factors, clinical manifestations and management options of ectopic pregnancy.Methods: This retrospective observational cohort study was done in the Department of Obstetrics and Gynaecology, at Sri Venkateshwaraa Medical College, Hospital and Research Centre, Ariyur, Puducherry, between January 2018- November 2021. Data was collected from the case sheets, operative notes and a retrospective analysis of the cohort of patients with ectopic pregnancy was done.Results: This retrospective study was conducted from January 2018 to November 2021 involving a total of 50 cases. We found that majority of tubal gestation occurred in the age group 26-30 years 58.6% and 13.7% in the age group more than 30 years. Majority of patients 65.5% underwent U/L salpingectomy.10.34% underwent salpingo oophorectomy. Fimbrial expression was done for two patients. Two patients underwent fimbriectomy. Cornual stump excision was done in two patients. Hemoperitoneum was detected in 55.17% of cases.Conclusion: Ectopic gestation can become an obstetrical emergency and reproductive capacity hampering morbidity if not diagnosed and treated on time. However having a high vigilance and evaluating every woman in the reproductive age group who presents with the classical clinical triad of amenorrhea, pain abdomen and bleeding per vagina can help in curbing the incidence in future.

Retrospective audit of genital prolapse management

Background: Pelvic organ prolapse is a descent of the pelvic organs into the vagina, frequently associated with local pelvic symptoms. Pelvic floor support is essential to maintain the normal anatomy. Recent studies of genital prolapse suggests that it is more important to improve the patient satisfaction and reduce complication than to achieve anatomical success. The purpose of the audit of genital prolapse management in this retrospective study is to know and understand the decision making, selection of patients for a particular surgery and to know and avoid the complications of genital prolapse management.Methods: This retrospective descriptive study was conducted at SVMCH and RC during the period of January 2020 to December 2021. The detailed analysis of the cases, symptomatology, surgical methods, complications and follow up were studied.Results: We managed 110 cases of genital prolapse. 35 patients had massive/ huge genital prolapse (POP Q Stage 4). 75.4%were found in the age group beyond 55 years, 66.3% were multiparous. 31% of the patients came with massive prolapse (POP Q-stage IV), 5% showed cervical elongation ,7% showed vault prolapse, Majority of our patients (63%) were managed by vaginal hysterectomy with pelvic floor repair. Followed by pessary, Fothergill’s surgery, Purandare’s surgery, Le forte’s, sacrospinal fixation, hysterosacropexy, pessary with thiersch stitch.Conclusions: The choice of management of genital prolapse has to be tailored according to the patients needs and pathology. The younger trainees have to be trained to get the skills of various surgeries of genital prolapse

Retrospective study of post-operative care in pandemic in tertiary care centre

Background: COVID-19 pandemic has unfortunately changed the surgical interventions and the post-operative management worldwide. Most of the medical institutes in India had formulated their own protocols from the beginning of the pandemic in March 2020 to stop all elective surgical procedures as soon as possible. However emergencies and all types of obstetric as cases were not stopped as it was unavoidable conditions.Methods: This retrospective observational study was done in the department of obstetrics and gynaecology, at Sri Venkateshwaraa Medical College, Ariyur, Puducherry, during the period April 2020-December 2020. Data was collected from the case sheets and operative notes and retrospective analysis was done.Results: On analysing we have realised that LSCS rate was higher and induction for trial of labour was kept minimal. We had 3 infective morbidity in the form of wound infection and 1 patient was returned to the operation theatre for reexploration. Arranging the blood during the pandemic was also a tedious task.Conclusions: In the present era of pandemic, where the patient load is high, we can rely on post graduate students to look after the post-operative patients through effective communication with the available consultants

A retrospective pilot study analyzing the effects of various factors and benefits of various drugs used in treating late admitted cases of acute ischemic stroke

Background: In Indian scenario, most of the cases of acute ischemic stroke are admitted and initiated treatment when the stipulated period of 4-6h for thrombolytic therapy is crossed. It is unclear on to benefits of treating such patients and our study aims at analysing the clinical and demographic factors associated with achieving improvement in functional ability in this special category of patients.Methods: Review of case records of acute ischemic stroke patients admitted after 6h of onset of stroke to a tertiary care teaching hospital of north Karnataka, India, over a period of one year with minimum of seven days of treatment was done. Drug utilization pattern, severity of ischemic stroke, baseline demographic and clinical data were extracted. The neurological recovery at the end of treatment period was analysed by using Barthel Index (BI) score. All the factors and drug utilization pattern were described using descriptive statistics and their correlation with BI score was analysed by spearman’s correlation coefficient.Results: Of the total 446 case records with diagnosis of stroke, only 39 met the eligibility criteria and included in the study. Statistically significant correlation to BI score was seen with severity of stroke, day-1 SBP, use of physiotherapy, time lapsed in initiating treatment, amount of decrease in SBP and baseline serum creatinine level.Conclusions: Our study helped in generating the hypothesis on effects of various factors and therapeutic modalities on neurological recovery of late admitted cases of acute ischemic stroke

Transient analysis of third-grade viscoelastic nanofluid flow external to a heated cylinder with buoyancy effects

Nanotechnology is rapidly embracing numerous areas of manufacturing and process engineering. New types of nanomaterials are being exploited to improve, for example, coating integrity, anti-corrosion characteristics and other features of fabricated components. Motivated by these developments, in the current study a mathematical model is developed for unsteady free-convective laminar flow of third-grade viscoelastic fluid (doped with nano-particles) from a semi-infinite vertical isothermal cylinder, as a model of thermal coating flow of a pipe geometry. Non-Newtonian behavior is simulated with the thermodynamically robust third grade Reiner-Rivlin model which accurately represents polymer fluids. Nanoscale effects are analyzed with the Buongiorno two-component nanofluid model. The governing equations comprise a set of highly coupled, nonlinear, multi-degree partial differential equations featuring viscoelastic and nanofluid parameters. An implicit Crank-Nicolson numerical scheme is implemented to solve the emerging nonlinear problem with appropriate initial and boundary conditions. Detailed graphical plots for velocity, temperature and nano-particle volume fraction are presented for a range of different parameters (i.e., third-grade fluid parameter, Brownian motion parameter, thermophoretic parameter, buoyancy ratio parameter, Lewis number). Additionally, distributions of the heat transfer coefficient, skin friction and Sherwood number at the cylinder surface are visualized. Furthermore, streamlines, isotherms and nano-particle volume fraction contour plots are included for variation of the third-grade parameter. Contour plots for the third-grade nanofluid flow are found to deviate significantly from those corresponding to Newtonian nanofluids. Validation of the numerical solutions with earlier studies is also included. KEYWORDS: Third-grade viscoelastic nanofluid, Thermal convection, Cylinder, Thermophoresis, Brownian motion, Implicit numerical method, Contour plots, Industrial coating

Unsteady free convective heat transfer in third-grade fluid flow from an isothermal vertical plate : a thermodynamic analysis

The current study investigates theoretically and numerically the entropy generation in time-dependent free-convective third-grade viscoelastic fluid convection flow from a vertical plate. The non-dimensional conservation equations for mass, momentum, and energy are solved using a Crank-Nicolson finite difference method with suitable boundary conditions. Expressions for known values of flow-variables coefficients are also derived for the wall heat transfer and skin friction and numerically evaluated. The effect of Grashof number, Prandtl number, group parameter (product of dimensionless temperature difference and Brinkman number) and third-grade parameter on entropy heat generation is analyzed and shown graphically. Bejan line distributions are also presented for the influence of several control parameters. The computations reveal that with increasing third-grade parameter the entropy generation decreases and Bejan number increases. Also, the comparison graph shows that contour lines for third-grade fluid vary considerably from the Newtonian fluid. The study is relevant to non-Newtonian thermal materials processing systems

A study of efficacy of ormeloxifene in abnormal uterine bleeding of ovulatory disorders

Background: Abnormal uterine bleeding (AUB) of ovulatory disorders is defined as the state of abnormal bleeding from the genital tract without any clinically detectable organic, systemic and iatrogenic cause. AUB is one of the common gynaecological issues among the patients visiting the hospital as it could affect at least 1/3rd of women in the child bearing age. Ormeloxifene, a nonsteroidal, nonhormonal and selective estrogen receptor modulator is a highly safe drug in treating acute and abnormal menstrual bleeding.   Methods: The 40 cases of heavy menstrual bleeding (HMB) with ovulatory disorder in the range of 40-52 years of age were treated with 60 mg ormeloxifene twice a week for first 12 weeks and once a week for next 12 weeks.  The patients were evaluated for haemoglobin, pictorial blood loss assessment chart (PBAC) score and endometrial thickness before and after treatment.Results: A significant decrease in endometrial thickness and PBAC score was observed. Also, the level of haemoglobin is found to increase significantly from 8.4±0.8 to 9.9±0.7. In this study, following 6 months of treatment with ormeloxifene, 90% of cases had marked improvement in their symptoms, while 10% of the patients required surgical management i.e., hysterectomy. Amenorrhea was observed in 7.5% of total cases and hypomenorrhea in 5% cases.Conclusions: Based on these results we could conclude that ormeloxifene could be the drug of choice in patients with AUB as it is very safe with manageable side effects. This simple drug-based therapy has facilitated compliance, tolerability and noticeable reduction of symptoms resulting in satisfaction towards the treatment