Nantes iPSC Core Facility

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Generation of Regulatory T Cells from Human Pluripotent Stem Cells

International audienceOrgan or cell transplantation is the only therapeutic solution for pathologies causing an irreversible loss of vital organs function. The development of novel specific and non-toxic ani-rejection immunotherapies is a major goal in transplantation. Strategies based on Tregs are promising. However, Tregs cellbased therapies have been hampered by the technical limitation of obtaining large batches of functional Tregs. The project aim is to obtain an unlimited number of Tregs from human pluripotent stem cells. This very innovative project will also allow us to beter understand the development biology of Tregs through their in vitro differentiation

Urine-derived cells provide a readily accessible cell type for feeder-free mRNA reprogramming

Abstract Over a decade after their discovery, induced pluripotent stem cells (iPSCs) have become a major biological model. The iPSC technology allows generation of pluripotent stem cells from somatic cells bearing any genomic background. The challenge ahead of us is to translate human iPSCs (hiPSCs) protocols into clinical treatment. To do so, we need to improve the quality of hiPSCs produced. In this study we report the reprogramming of multiple patient urine-derived cell lines with mRNA reprogramming, which, to date, is one of the fastest and most faithful reprogramming method. We show that mRNA reprogramming efficiently generates hiPSCs from urine-derived cells. Moreover, we were able to generate feeder-free bulk hiPSCs lines that did not display genomic abnormalities. Altogether, this reprogramming method will contribute to accelerating the translation of hiPSCs to therapeutic applications

Parallel derivation of isogenic human primed and naive induced pluripotent stem cells

Induced pluripotent stem cells (iPSCs) have considerably impacted human developmental biology and regenerative medicine, notably because they circumvent the use of cells from embryonic origin and offer the potential to generate patient-specific pluripotent stem cells. However, conventional reprogramming protocols produce developmentally advanced, or primed, human iPSCs (hiPSCs), restricting their use to postimplantation human development modeling. Hence, there is a need for hiPSCs resembling preimplantation naive epiblast. Here, we develop a method to generate naive hiPSCs directly from somatic cells, using OKMS overexpression and specific culture conditions, further enabling parallel generation of their isogenic primed counterparts. We benchmark naive hiPSCs against human preimplantation epiblast and reveal a remarkable concordance in their transcriptome, dependency on mitochondrial respiration and X chromosome status. Collectively, our results are essential for the understanding of pluripotency regulation throughout preimplantation development and generate new opportunities for disease modeling and regenerative medicine.status: publishe

A dominant vimentin variant causes a rare syndrome with premature aging

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