Changes in MEG resting-state networks are related to cognitive decline in type 1 diabetes mellitus patients

OBJECTIVE: Integrity of resting-state functional brain networks (RSNs) is important for proper cognitive functioning. In type 1 diabetes mellitus (T1DM) cognitive decrements are commonly observed, possibly due to alterations in RSNs, which may vary according to microvascular complication status. Thus, we tested the hypothesis that functional connectivity in RSNs differs according to clinical status and correlates with cognition in T1DM patients, using an unbiased approach with high spatio-temporal resolution functional network.; METHODS: Resting-state magnetoencephalographic (MEG) data for T1DM patients with (n=42) and without (n=41) microvascular complications and 33 healthy participants were recorded. MEG time-series at source level were reconstructed using a recently developed atlas-based beamformer. Functional connectivity within classical frequency bands, estimated by the phase lag index (PLI), was calculated within eight commonly found RSNs. Neuropsychological tests were used to assess cognitive performance, and the relation with RSNs was evaluated.; RESULTS: Significant differences in terms of RSN functional connectivity between the three groups were observed in the lower alpha band, in the default-mode (DMN), executive control (ECN) and sensorimotor (SMN) RSNs. T1DM patients with microvascular complications showed the weakest functional connectivity in these networks relative to the other groups. For DMN, functional connectivity was higher in patients without microangiopathy relative to controls (all p<0.05). General cognitive performance for both patient groups was worse compared with healthy controls. Lower DMN alpha band functional connectivity correlated with poorer general cognitive ability in patients with microvascular complications.; DISCUSSION: Altered RSN functional connectivity was found in T1DM patients depending on clinical status. Lower DMN functional connectivity was related to poorer cognitive functioning. These results indicate that functional connectivity may play a key role in T1DM-related cognitive dysfunction

The effect of cellulose nanocrystals on latex and adhesive properties in emulsion- based polymer nanocomposites

Pressure sensitive adhesives (PSAs) adhere quickly and firmly to surfaces with the application of light pressure, and can be removed without leaving a residue. Their mechanical performance is measured by tack, peel strength and shear strength. A balanced combination between the three mechanical performance measurements depends on the specific end-use application and is challenging to achieve. This is particularly so when replacing solvent-based technologies with more sustainable, water-based (i.e., emulsion polymerization) technologies. PSAs synthesized using emulsion polymerization tend to have a lower shear strength due to poor gel network formation. As a result, conventional emulsion-based PSAs suffer from the inability to increase certain adhesive properties (e.g., tack and peel strength) while simultaneously increasing shear strength. Nanomaterials are often used in polymer composites to improve polymer properties (e.g., tensile strength). They are particularly effective in low quantities (e.g., \u3c2 \u3ewt.%) because of their high surface area. Cellulose nanocrystals (CNCs) are a “green alternative” to common nanomaterials and are isolated from natural cellulose. CNCs have been used more commonly, in the past, as rheological modifiers and interface stabilizers.[1] Because CNCs form colloidally stable dispersions in water, they can be incorporated/processed in water-based systems, eliminating the need for organic solvents.[2] The most common method to produce CNCs is through acid hydrolysis with sulfuric acid; this process preferentially degrades the disordered cellulose regions and leaves behind the crystalline CNCs with grafted anionic sulfate half ester groups.[1] The resulting nanoparticles are whisker-shaped and have a high aspect ratio.[3] CNCs provide composite material reinforcement in the range of other nanomaterials. In the past, CNCs have been blended with polymers and significant strength improvements were noted.[4] Our studies demonstrate how to incorporate CNCs in a nanocomposite using an in situ semi-batch emulsion polymerization protocol.[5] PSA nanocomposite films were generated for a broad variety of copolymer systems including monomers such as iso-butyl acrylate, n-butyl acrylate, 2-ethyl hexyl acrylate, methyl methacrylate, styrene and vinyl acetate. In all cases, the monomer composition of the reaction formulations was manipulated to achieve a suitable range of polymer glass transition temperatures. CNC loadings were varied from 0 to 0.5 to 1 wt.% (based on monomer weight). The addition of CNC was shown to significantly and simultaneously increase tack, peel strength, and shear strength.[6] References [1] Dufresne, A., Nanocellulose, De Gruyter, Saint Martin D’Heres Cedex, France 2012. [2] Flauzino Neto, W. P., Mariano, M., da Silva, I. S. V., Silvério, H. A., Putaux, J.-L., Otaguro, H., Pasquini, D., Dufresne, A., Carbohydr. Polym. 2016, 153, 143. [3] Moon, R. J., Martini, A., Nairn, J., Simonsen, J., Youngblood, J., Chem. Soc. Rev., 2011, 40, 3941. [4] Rajisha, K. R., Maria, H. J., Pothan, L. A., Ahmad, Z., Thomas, S., Int. J. Biol. Macromol., 2014, 67, 147. [5] Dastjerdi, Z., Cranston, E. D., Dubé, M. A., Macromol. React. Eng., 2018, in press. [6] Dastjerdi, Z., Cranston, E. D., Dubé, M. A., Int. J. Adh. Adh. 2018, 81, 36-42

Wearable System Based on Ultra-Thin Parylene C Tattoo Electrodes for EEG Recording

In an increasingly interconnected world, where electronic devices permeate every aspect of our lives, wearable systems aimed at monitoring physiological signals are rapidly taking over the sport and fitness domain, as well as biomedical fields such as rehabilitation and prosthetics. With the intent of providing a novel approach to the field, in this paper we discuss the development of a wearable system for the acquisition of EEG signals based on a portable, low-power custom PCB specifically designed to be used in combination with non-conventional ultra-conformable and imperceptible Parylene-C tattoo electrodes. The proposed system has been tested in a standard rest-state experiment, and its performance in terms of discrimination of two different states has been compared to that of a commercial wearable device for EEG signal acquisition (i.e., the Muse headset), showing comparable results. This first preliminary validation demonstrates the possibility of conveniently employing ultra-conformable tattoo-electrodes integrated portable systems for the unobtrusive acquisition of brain activity

The Golgi apparatus is a primary site of intracellular damage after photosensitization with Rose Bengal acetate

The aim of the present investigation was to elucidate whether the Golgi apparatus undergoes photodamage following administration of the fluorogenic substrates Rose Bengal acetate (RBAc) and irradiation at the appropriate wavelength. Human HeLa cells were treated in culture and the changes in the organization of the Golgi apparatus were studied using fluorescence confocal microscopy and electron microscopy, after immunocytochemical labeling. To see whether the cytoskeletal components primarily involved in vescicle traffic (i.e., microtubules) might also be affected, experiments of tubulin immunolabeling were performed. After treatment with RBAc and irradiation, cells were allowed to grow in drug-free medium for different times. 24hr after irradiation, the cisternae of the Golgi apparatus became packed, and after 48-72 hr they appeared more fragmented and scattered throughout the cytoplasm; these changes in the organization of the Golgi cisternae were confirmed at electron microscopy. Interestingly enough, apoptosis was found to occur especially 48-72h after irradiation, and apoptotic cells exhibited a dramatic fragmentation of the Golgi membranes. The immunolabeling with anti-tubulin antibody showed that microtubules were also affected by irradiation in RBAc-treated cells

Pressure sensitive adhesives produced by in-situ emulsion polymerization of cellulose nanocrystal-poly(nBA-VAc)

Pressure sensitive adhesives (PSAs) are conventionally produced using a variety of polymerization methods such as emulsion, solution, or radiation curing. Environmental concerns favor the development of emulsion polymerization based PSAs.[1] However, maintaining and controlling the PSA properties achievable from solution polymerization in PSAs produced by emulsion polymerization remains challenging. Depending on the particular adhesive application, PSA properties are largely guided by the polymer glass transition temperature and the polymer microstructure. The latter is controlled in a variety of ways but typically via the addition of chain transfer agents and crosslinkers.[2] During the last decades, efforts in PSA property manipulation have included the preparation of nanocomposite latexes by introducing nanomaterials such as titanium dioxide, silica, and carbon nanotubes into the formulations.[3] On the other hand, utilizing cellulose nanocrystals (CNCs) as a sustainable source of reinforcement in polymers is emerging rapidly.[4] CNCs are the product of controlled hydrolysis of plant based tissues, through which crystalline domains of cellulose are isolated from the disordered parts of the raw material. High aspect ratio, surface activity and modulus, as well as non-toxic nature of CNCs make them ideal candidates for use in nanocomposite formulations. More recently, our group have prepared CNC nanocomposite PSAs which were revealed to significantly and simultaneously improve tack, peel strength and shear strength in the PSA films.[5] The ability to improve tack and peel strength without decreasing shear strength overcomes a major challenge in PSA formulation. We will present results from emulsion polymerization of n-butyl acrylate/vinyl acetate/CNC nanocomposite PSAs. We will identify the location of the CNCs relative to the latex particles and show their effect on latex viscosity, gel content, and PSA properties. The goal of these new results is to show how the manipulation of the reaction formulation (e.g., monomer feed ratio, surfactant type) will affect the distribution and relative location of the CNCs in the polymer latex and ultimately the PSA properties. [1] Jovanović, R., Dubé, M. A., J. Macromol. Sci., Part C, 44:1, 1-51, 2004. [2] Qie, L., Dubé, M. A., 46, 1225–1236, 2010. [3] Dastjerdi, Z., Cranston, E. D., Berry, R. Fraschini, C., Dubé, M. A., J. Matls. Sci., submitted January 2018. [4] Lee, K-Y., Aitomäki, Y., Berglund, L. A., Oksman, K., Bismarck, A., Compos. Sci. Technol. 105, 15–27, 2014. [5] Dastjerdi, Z., Cranston, E. D., Dubé, M. A., Macromol. React. Eng., 11, 1700013, 2017. [6] Dastjerdi, Z., Cranston, E. D., Dubé, M. A., Int. J. Adh. Adh., 81, 36-42, 201

Reshaping cortical connectivity in traumatic spinal cord injury: a novel effect of hyperbaric oxygen therapy

Introduction: Spinal cord injuries (SCIs) represent a severe neuro-traumatic occurrence and an excruciating social burden. Though the hyperbaric oxygen (HBO2) has been credited as a first line therapeutic resource for SCIs, its mechanism of action in the spine is only partially known, while the impingement upon other areas of the nervous system deserves additional investigation. In this study we deem to describe a novel effect of HBO2 in a subject affected by SCI who, along with the clinical improvement, showed a reshaped connectivity in cortical sensory-motor areas. Case presentation: A 45 years male presenting severe sensory-motor symptoms following a spinal lesion partially involving the C1 segment was successfully treated with HBO2 cycles. After the dramatic improvement reflected by an excellent optimization of the single performances, it has been investigated whether this result would reveal not only an intrinsic effect upon the spinal cord, but also a better connectivity strength in sensory-motor cortical regions. The results obtained by implementing EEG recordings with EEGLAB auto regressive vector plugins indeed suggest a substantial reshaping of cortico-cortical connectivity after HBO2. Discussion: These results show a correlation between positive clinical evolution and a new modulation of cortical connectivity. Though further clinical investigations would clarify as to whether HBO2 might be directly or epiphenomenally involved in this aspect of the network architecture, our report suggests that a comparison between clinical results and the study of brain connectivity represent a holistic approach in investigating the physiopathology of SCIs and in monitoring the treatment

P217 In vitro study on a tissue engineered osteochondral composite

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COG7 deficiency in Drosophila generates multifaceted developmental, behavioral and protein glycosylation phenotypes

Congenital disorders of glycosylation (CDG) comprise a family of human multisystemic diseases caused by recessive mutations in genes required for protein N-glycosylation. More than 100 distinct forms of CDGs have been identified and most of them cause severe neurological impairment. The Conserved Oligomeric Golgi (COG) complexmediates tethering of vesicles carrying glycosylation enzymes across the Golgi cisternae. Mutations affecting human COG1, COG2 and COG4-COG8 cause monogenic forms of inherited, autosomal recessive CDGs.We have generated a Drosophila COG7-CDG model that closely parallels the pathological characteristics of COG7-CDG patients, including pronounced neuromotor defects associated with altered N-glycome profiles. Consistent with these alterations, larval neuromuscular junctions of Cog7 mutants exhibit a significant reduction in bouton numbers. We demonstrate that the COG complex cooperates with Rab1 and Golgi phosphoprotein 3 to regulate Golgi trafficking and that overexpression of Rab1 can rescue the cytokinesis and locomotor defects associated with loss of Cog7. Our results suggest that the Drosophila COG7-CDG model can be used to test novel potential therapeutic strategies by modulating trafficking pathways