320 research outputs found
FACT - Threshold prediction for higher duty cycle and improved scheduling
The First G-APD Cherenkov telescope (FACT) is the first telescope using
silicon photon detectors (G-APD aka. SiPM). The use of Silicon devices promise
a higher photon detection efficiency, more robustness and higher precision than
photo-multiplier tubes. Being operated during different light-conditions, the
threshold settings of a Cherenkov telescope have to be adapted to feature the
lowest possible threshold but also an efficient suppression of triggers from
night-sky background photons. Usually this threshold is set either by
experience or a mini-ratescan. Since the measured current through the sensors
is directly correlated with the noise level, the current can be used to set the
best threshold at any time. Due to the correlation between the physical
threshold and the final energy threshold, the current can also be used as a
measure for the energy threshold of any observation. This presentation
introduces a method which uses the properties of the moon and the source
position to predict the currents and the corresponding energy threshold for
every upcoming observation allowing to adapt the observation schedule
accordingly
FACT - Long-term stability and observations during strong Moon light
The First G-APD Cherenkov Telescope (FACT) is the first Cherenkov telescope
equipped with a camera made of silicon photon detectors (G-APD aka. SiPM).
Since October 2011, it is regularly taking data on the Canary Island of La
Palma. G-APDs are ideal detectors for Cherenkov telescopes as they are robust
and stable. Furthermore, the insensitivity of G-APDs towards strong ambient
light allows to conduct observations during bright Moon and twilight. This gain
in observation time is essential for the long-term monitoring of bright TeV
blazars. During the commissioning phase, hundreds of hours of data (including
data from the the Crab Nebula) were taken in order to understand the
performance and sensitivity of the instrument. The data cover a wide range of
observation conditions including different weather conditions, different zenith
angles and different light conditions (ranging from dark night to direct full
Moon). We use a new parmetrisation of the Moon light background to enhance our
scheduling and to monitor the atmosphere. With the data from 1.5 years, the
long-term stability and the performance of the camera during Moon light is
studied and compared to that achieved with photomultiplier tubes so far.Comment: 3 pages, 3 figures, FACT Contribution to the 33rd International
Cosmic Ray Conference (ICRC), Rio de Janeir
FACT - Long-term Monitoring of Bright TeV-Blazars
Since October 2011, the First G-APD Cherenkov Telescope (FACT) is operated
successfully on the Canary Island of La Palma. Apart from the proof of
principle for the use of G-APDs in Cherenkov telescopes, the major goal of the
project is the dedicated long-term monitoring of a small sample of bright TeV
blazars. The unique properties of G-APDs permit stable observations also during
strong moon light. Thus a superior sampling density is provided on time scales
at which the blazar variability amplitudes are expected to be largest, as
exemplified by the spectacular variations of Mrk 501 observed in June 2012.
While still in commissioning, FACT monitored bright blazars like Mrk 421 and
Mrk 501 during the past 1.5 years so far. Preliminary results including the Mrk
501 flare from June 2012 will be presented.Comment: 4 pages, 4 figures, presented at the 33rd ICRC (2013
FACT - How stable are the silicon photon detectors?
The First G-APD Cherenkov telescope (FACT) is the first telescope using
silicon photon detectors (G-APD aka. SiPM). The use of Silicon devices promise
a higher photon detection efficiency, more robustness and higher precision than
photo-multiplier tubes. Since the properties of G-APDs depend on auxiliary
parameters like temperature, a feedback system adapting the applied voltage
accordingly is mandatory.
In this presentation, the feedback system, developed and in operation for
FACT, is presented. Using the extraction of a single photon-equivalent (pe)
spectrum as a reference, it can be proven that the sensors can be operated with
very high precision. The extraction of the single-pe, its spectrum up to
10\,pe, its properties and their precision, as well as their long-term behavior
during operation are discussed. As a by product a single pulse template is
obtained. It is shown that with the presented method, an additional external
calibration device can be omitted. The presented method is essential for the
application of G-APDs in future projects in Cherenkov astronomy and is supposed
to result in a more stable and precise operation than possible with
photo-multiplier tubes
Individual Profiling of Circulating Tumor Cell Composition and Therapeutic Outcome in Patients with Hepatocellular Carcinoma
AbstractBACKGROUND AND AIMS: Circulating tumor cells (CTCs) have been proposed as a monitoring tool in patients with solid tumors. So far, automated approaches are challenged by the cellular heterogeneity of CTC, especially the epithelial-mesenchymal transition. Recently, Yu and colleagues showed that shifts in these cell populations correlated with response and progression, respectively, to chemotherapy in patients with breast cancer. In this study, we assessed which non-hematopoietic cell types were identifiable in the peripheral blood of hepatocellular carcinoma (HCC) patients and whether their distribution during treatment courses is associated with clinical characteristics. METHODS: Subsequent to few enrichment steps, cell suspensions were spun onto glass slides and further characterized using multi-immunofluorescence staining. All non-hematopoietic cells were counted and individual cell profiles were analyzed per patient and treatment. RESULTS:We detected a remarkable variation of cells with epithelial, mesenchymal, liver-specific, and mixed characteristics and different size ranges. The distribution of these subgroups varied significantly between different patient groups and was associated with therapeutic outcome. Kaplan-Meier logrank test showed that a change in the ratio of epithelial to mesenchymal cells was associated with longer median time to progression (1 vs 15 months; P = .03; hazard ratio = 0.18; 95%confidence interval = 0.01-2.75). CONCLUSIONS: Our data suggest that different CTC populations are identifiable in peripheral blood of HCC patients and, for the first time in HCC, that these individual cell type profiles may have distinct clinical implications. The further characterization and analysis of patients in this ongoing study seems to be warranted
A generally applicable lightweight method for calculating a value structure for tools and services in bioinformatics infrastructure projects
Sustainable noncommercial bioinformatics infrastructures are a prerequisite to use and take advantage of the potential of big data analysis for research and economy. Consequently, funders, universities and institutes as well as users ask for a transparent value model for the tools and services offered. In this article, a generally applicable lightweight method is described by which bioinformatics infrastructure projects can estimate the value of tools and services offered without determining exactly the total costs of ownership. Five representative scenarios for value estimation from a rough estimation to a detailed breakdown of costs are presented. To account for the diversity in bioinformatics applications and services, the notion of service-specific âservice provision unitsâ is introduced together with the factors influencing them and the main underlying assumptions for these âvalue influencing factorsâ. Special attention is given on how to handle personnel costs and indirect costs such as electricity. Four examples are presented for the calculation of the value of tools and services provided by the German Network for Bioinformatics Infrastructure (de.NBI): one for tool usage, one for (Web-based) database analyses, one for consulting services and one for bioinformatics training events. Finally, from the discussed values, the costs of direct funding and the costs of payment of services by funded projects are calculated and compared
Calibration and performance of the photon sensor response of FACT -- The First G-APD Cherenkov telescope
The First G-APD Cherenkov Telescope (FACT) is the first in-operation test of
the performance of silicon photo detectors in Cherenkov Astronomy. For more
than two years it is operated on La Palma, Canary Islands (Spain), for the
purpose of long-term monitoring of astrophysical sources. For this, the
performance of the photo detectors is crucial and therefore has been studied in
great detail. Special care has been taken for their temperature and voltage
dependence implementing a correction method to keep their properties stable.
Several measurements have been carried out to monitor the performance. The
measurements and their results are shown, demonstrating the stability of the
gain below the percent level. The resulting stability of the whole system is
discussed, nicely demonstrating that silicon photo detectors are perfectly
suited for the usage in Cherenkov telescopes, especially for long-term
monitoring purpose
FACT -- Operation of the First G-APD Cherenkov Telescope
Since more than two years, the First G-APD Cherenkov Telescope (FACT) is
operating successfully at the Canary Island of La Palma. Apart from its purpose
to serve as a monitoring facility for the brightest TeV blazars, it was built
as a major step to establish solid state photon counters as detectors in
Cherenkov astronomy.
The camera of the First G-APD Cherenkov Telesope comprises 1440 Geiger-mode
avalanche photo diodes (G-APD aka. MPPC or SiPM) for photon detection. Since
properties as the gain of G-APDs depend on temperature and the applied voltage,
a real-time feedback system has been developed and implemented. To correct for
the change introduced by temperature, several sensors have been placed close to
the photon detectors. Their read out is used to calculate a corresponding
voltage offset. In addition to temperature changes, changing current introduces
a voltage drop in the supporting resistor network. To correct changes in the
voltage drop introduced by varying photon flux from the night-sky background,
the current is measured and the voltage drop calculated. To check the stability
of the G-APD properties, dark count spectra with high statistics have been
taken under different environmental conditions and been evaluated.
The maximum data rate delivered by the camera is about 240 MB/s. The recorded
data, which can exceed 1 TB in a moonless night, is compressed in real-time
with a proprietary loss-less algorithm. The performance is better than gzip by
almost a factor of two in compression ratio and speed. In total, two to three
CPU cores are needed for data taking. In parallel, a quick-look analysis of the
recently recorded data is executed on a second machine. Its result is publicly
available within a few minutes after the data were taken.
[...]Comment: 19th IEEE Real-Time Conference, Nara, Japan (2014
PRIDE: Quality control in a proteomics data repository
The PRoteomics IDEntifications (PRIDE) database is a large public proteomics data repository, containing over 270 million mass spectra (by November 2011). PRIDE is an archival database, providing the proteomics data supporting specific scientific publications in a computationally accessible manner. While PRIDE faces rapid increases in data deposition size as well as number of depositions, the major challenge is to ensure a high quality of data depositions in the context of highly diverse proteomics work flows and data representations. Here, we describe the PRIDE curation pipeline and its practical application in quality control of complex data depositions
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