Virus Movement in Groundwater Systems

The purpose of this study is to investigate the extent to which soil acts as an agent in the transmission of waterborne viruses. Since many waterborne outbreaks of viral diseases have involved small wellwater supplies contaminated by effluents from subsurface wastewater disposal systems, there is a great need for such information. Results of this study show that virus adsorption by soils is greatly affected by the pH, ionic strength, and soil-water ratio of the soil-water system and various soil properties. Also, it is shown that one cannot predict the relative virus adsorbing ability of a particular soil based on the various tests normally used to characterize a soil. It is shown that virus movement through a continuous stratum of common soil under gravity flow conditions and with intermittent dosing should present no health hazard if usual public health practices relating to locating water supply wells are followed. Test results also indicate no greater or lesser movement of virus through soils with a highly polluted water than with a non-polluted water

The cost of wisdom : a comparative theoretical analysis of research ethics in the United States, Germany and Hungary.

This dissertation is a comparative theoretical analysis of human rights, research ethics and human subjects protection. The history of human rights is a long one, but for the sake of immediacy and focus, the author will limit the discussion to events pertaining to the last century and the beginning of a new one. Through a process of sociocultural review of significant human rights and scientific events pertinent to research involving human subjects, this work will examine the evolution of research ethics in the United States, Germany and Hungary. These nations are not intended to be representative of international research ethics as a whole, but their 20th century histories starkly depict how the forces of democracy, national socialism and communism have shaped our view of how science has been used to transform societies and our beliefs regarding the human condition. The author intends to place these societies and the research topic in a theoretical context that will add depth and resonance to the discussion by examining how the aforementioned political schemes, and the ethical codes which have arisen within them, came to produce the greatness and tragedy documented in these pages. The theories to be used in this analysis are structural functionalism, general systems theory, conflict theory, Peter Blau\u27s exchange theory of power, and symbolic interactionism. The art and science of social work stand amidst the issue of how societies come to address the ethics of human subjects protection in research. With its historic dedication to the protection of human rights and the enfranchisement of all peoples within and across societies, social work is positioned to negotiate and implement the principles codified into the traditions and laws governing international human subjects protection for research participants. The dissertation will begin with sociohistorical review of the nations under study to provide necessary context and information about the research topic. A theoretical analysis will follow. The dissertation will include a recommendation for a code of research ethics which has broad applicability across cultures, and conclude with a discussion of the role of the social work profession in its operationalization

Chapter 5: What Books Are in Your Card Catalog?

[First paragraph] Author’s note: In 1970, Richard Nelson Bolles self-published a book about searching for a job entitled, What Color is Your Parachute? Bolles’ seminal work on job searching was so well received that it has been updated and republished annually since 1975 (Wikipedia, 2022). The title of this chapter, What Books are in Your Card Catalog?, is an homage to that work. Unless you are over the age of 40, the card catalog may not seem relevant due to the replacement of most card catalogs with the online catalog. However, the goal of the card catalog was to enable the user to find a book in the library by using the title, author, or subject as an identifier. The goal for this chapter is that the reader will be able to use the tools herein to find a job as a librarian in a school, academic, public, or private setting that will be enriching and satisfying for years to come

Determination of diaphragm opening-times and use of diaphragm particle traps in a hypersonic shock tube

Determination of diaphragm opening-times and use of diaphragm particle traps in hypersonic shock tub

Lava channel formation during the 2001 eruption on Mount Etna: evidence for mechanical erosion

We report the direct observation of a peculiar lava channel that was formed near the base of a parasitic cone during the 2001 eruption on Mount Etna. Erosive processes by flowing lava are commonly attributed to thermal erosion. However, field evidence strongly suggests that models of thermal erosion cannot explain the formation of this channel. Here, we put forward the idea that the essential erosion mechanism was abrasive wear. By applying a simple model from tribology we demonstrate that the available data agree favorably with our hypothesis. Consequently, we propose that erosional processes resembling the wear phenomena in glacial erosion are possible in a volcanic environment.Comment: accepted for publication in Physical Review Letter

1,2,6-thiadiazinones as novel narrow spectrum calcium/calmodulin-dependent protein kinase kinase 2 (CaMKK2) inhibitors

We demonstrate for the first time that 4H-1,2,6-thiadiazin-4-one (TDZ) can function as a chemotype for the design of ATP-competitive kinase inhibitors. Using insights from a co-crystal structure of a 3,5-bis(arylamino)-4H-1,2,6-thiadiazin-4-one bound to calcium/calmodulin-dependent protein kinase kinase 2 (CaMKK2), several analogues were identified with micromolar activity through targeted displacement of bound water molecules in the active site. Since the TDZ analogues showed reduced promiscuity compared to their 2,4-dianilinopyrimidine counter parts, they represent starting points for development of highly selective kinase inhibitors

Comparison of monocyte human leukocyte antigen-DR expression and stimulated tumor necrosis factor alpha production as outcome predictors in severe sepsis: A prospective observational study

BACKGROUND: Identifying patients in the immunosuppressive phase of sepsis is essential for development of immunomodulatory therapies. Little data exists comparing the ability of the two most well-studied markers of sepsis-induced immunosuppression, human leukocyte antigen (HLA)-DR expression and lipopolysaccharide (LPS)-induced tumor necrosis factor alpha (TNF-ɑ) production, to predict mortality and morbidity. The purpose of this study was to compare HLA-DR expression and LPS-induced TNF-ɑ production as predictors of 28-day mortality and acquisition of secondary infections in adult septic patients. METHODS: A single-center, prospective observational study of 83 adult septic patients admitted to a medical or surgical intensive care unit. Blood samples were collected at three time points during the septic course (days 1–2, days 3–4, and days 6–8 after sepsis diagnosis) and assayed for HLA-DR expression and LPS-induced TNF-ɑ production. A repeated measures mixed model analysis was used to compare values of these immunological markers among survivors and non-survivors and among those who did and did not develop a secondary infection. RESULTS: Twenty-five patients (30.1 %) died within 28 days of sepsis diagnosis. HLA-DR expression was significantly lower in non-survivors as compared to survivors on days 3–4 (p = 0.04) and days 6–8 (p = 0.002). The change in HLA-DR from days 1–2 to days 6–8 was also lower in non-survivors (p = 0.04). Median HLA-DR expression decreased from days 1–2 to days 3–4 in patients who developed secondary infections while it increased in those without secondary infections (p = 0.054). TNF-ɑ production did not differ between survivors and non-survivors or between patients who did and did not develop a secondary infection. CONCLUSIONS: Monocyte HLA-DR expression may be a more accurate predictor of mortality and acquisition of secondary infections than LPS-stimulated TNF-ɑ production in adult medical and surgical critically ill patients. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s13054-016-1505-0) contains supplementary material, which is available to authorized users

Formation of Structure in Snowfields: Penitentes, Suncups, and Dirt Cones

Penitentes and suncups are structures formed as snow melts, typically high in the mountains. When the snow is dirty, dirt cones and other structures can form instead. Building on previous field observations and experiments, this work presents a theory of ablation morphologies, and the role of surface dirt in determining the structures formed. The glaciological literature indicates that sunlight, heating from air, and dirt all play a role in the formation of structure on an ablating snow surface. The present work formulates a mathematical model for the formation of ablation morphologies as a function of measurable parameters. The dependence of ablation morphologies on weather conditions and initial dirt thickness are studied, focusing on the initial growth of perturbations away from a flat surface. We derive a single-parameter expression for the melting rate as a function of dirt thickness, which agrees well with a set of measurements by Driedger. An interesting result is the prediction of a dirt-induced travelling instability for a range of parameters.Comment: 28 pages, 13 figure

T cells from patients with Candida sepsis display a suppressive immunophenotype

BACKGROUND: Despite appropriate therapy, Candida bloodstream infections are associated with a mortality rate of approximately 40 %. In animal models, impaired immunity due to T cell exhaustion has been implicated in fungal sepsis mortality. The purpose of this study was to determine potential mechanisms of fungal-induced immunosuppression via immunophenotyping of circulating T lymphocytes from patients with microbiologically documented Candida bloodstream infections. METHODS: Patients with blood cultures positive for any Candida species were studied. Non-septic critically ill patients with no evidence of bacterial or fungal infection were controls. T cells were analyzed via flow cytometry for cellular activation and for expression of positive and negative co-stimulatory molecules. Both the percentages of cells expressing particular immunophenotypic markers as well as the geometric mean fluorescence intensity (GMFI), a measure of expression of the number of receptors or ligands per cell, were quantitated. RESULTS: Twenty-seven patients with Candida bloodstream infections and 16 control patients were studied. Compared to control patients, CD8 T cells from patients with Candidemia had evidence of cellular activation as indicated by increased CD69 expression while CD4 T cells had decreased expression of the major positive co-stimulatory molecule CD28. CD4 and CD8 T cells from patients with Candidemia expressed markers typical of T cell exhaustion as indicated by either increased percentages of or increased MFI for programmed cell death 1 (PD-1) or its ligand (PD-L1). CONCLUSIONS: Circulating immune effector cells from patients with Candidemia display an immunophenotype consistent with immunosuppression as evidenced by T cell exhaustion and concomitant downregulation of positive co-stimulatory molecules. These findings may help explain why patients with fungal sepsis have a high mortality despite appropriate antifungal therapy. Development of immunoadjuvants that reverse T cell exhaustion and boost host immunity may offer one way to improve outcome in this highly lethal disorder. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s13054-016-1182-z) contains supplementary material, which is available to authorized users