A Fuzzy Approach to Sequential Failure Analysis Using Petri nets

In highly competitive industrial market, the concept of failure analysis is an unavoidable fact in complex industrial systems. Reliability of such systems not only depends on the reliability of each element of these systems, but also depends on occurrence of sequence of failures. In this paper, a novel approach to sequential failure analysis is proposed which is based upon fuzzy logic and the concept of Petri nets which is utilized to track all the risky behaviors of the system and to determine the potential failure sequences and then prioritizing them in order to perform corrective actions. The process of prioritizing failure sequences in this paper is done by a novel similarity measure between generalized fuzzy numbers. The proposed methodology is demonstrated with an example of two automated machine tools and two input/output buffer stocks

Deconvolution of transcriptional networks identifies TCF4 as a master regulator in schizophrenia

Applying tissue-specific deconvolution of transcriptional networks to identify their master regulators (MRs) in neuropsychiatric disorders has been largely unexplored. Here, using two schizophrenia (SCZ) case-control RNA-seq datasets, one on postmortem dorsolateral prefrontal cortex (DLPFC) and another on cultured olfactory neuroepithelium, we deconvolved the transcriptional networks and identified TCF4 as a top candidate MR that may be dysregulated in SCZ. We validated TCF4 as a MR through enrichment analysis of TCF4-binding sites in induced pluripotent stem cell (hiPSC)-derived neurons and in neuroblastoma cells. We further validated the predicted TCF4 targets by knocking down TCF4 in hiPSC-derived neural progenitor cells (NPCs) and glutamatergic neurons (Glut_Ns). The perturbed TCF4 gene network in NPCs was more enriched for pathways involved in neuronal activity and SCZ-associated risk genes, compared to Glut_Ns. Our results suggest that TCF4 may serve as a MR of a gene network dysregulated in SCZ at early stages of neurodevelopment