Integrating nursing theory, practice and research through collaborative research

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/75657/1/j.1365-2648.1989.tb00912.x.pd

A proposed new bacteriophage subfamily: “Jerseyvirinae”

© 2015, Springer-Verlag Wien. Based on morphology and comparative nucleotide and protein sequence analysis, a new subfamily of the family Siphoviridae is proposed, named “Jerseyvirinae” and consisting of three genera, “Jerseylikevirus”, “Sp3unalikevirus” and “K1glikevirus”. To date, this subfamily consists of 18 phages for which the genomes have been sequenced. Salmonella phages Jersey, vB_SenS_AG11, vB_SenS-Ent1, vB_SenS-Ent2, vB_SenS-Ent3, FSL SP-101, SETP3, SETP7, SETP13, SE2, SS3e and wksl3 form the proposed genus “Jerseylikevirus”. The proposed genus “K1glikevirus” consists of Escherichia phages K1G, K1H, K1ind1, K1ind2 and K1ind3. The proposed genus “Sp3unalikevirus” contains one member so far. Jersey-like phages appear to be widely distributed, as the above phages were isolated in the UK, Canada, the USA and South Korea between 1970 and the present day. The distinguishing features of this subfamily include a distinct siphovirus morphotype, genomes of 40.7-43.6kb (49.6-51.4mol% G+C), a syntenic genome organisation, and a high degree of nucleotide sequence identity and shared proteins. All known members of the proposed subfamily are strictly lytic

The genome and proteome of Serratia bacteriophage η which forms unstable lysogens

Background:Serratia marcescensphageηis a temperate unclassified member of theSiphoviridaewhich had beenreported as containing hypermodified guanine residues. Methods:The DNA was characterized by enzymatic digestion followed by HPLC analysis of the nucleosidecomposition, and by DNA sequencing and proteomic analysis. Its ability to form stable lysogens and integrate wasalso investigated. Results:Enzymatic digestion and HPLC analysis revealed phageηDNA did not contain modified bases. Thegenome sequence of this virus, determined using pyrosequencing, is 42,724 nucleotides in length with a mol% GCof 49.9 and is circularly permuted. Sixty-nine putative CDSs were identified of which 19 encode novel proteins.While seven close genetic relatives were identified, they shared sequence similarity with only genes40to69of thephageηgenome, while gp1 to gp39 shared no conserved relationship. The structural proteome, determined bySDS-PAGE and mass spectrometry, revealed seven unique proteins. This phage forms very unstable lysogens withits hostS. marcescens.ISSN:1743-422