In vitro antimicrobial and antiproliferative activity of amphipterygium adstringens

Amphipterygium adstringens is a plant widely used in Mexican traditional medicine for its known anti-inflammatory and antiulcer properties. In this work, we evaluated the in vitro antimicrobial and antiproliferative activities of the methanolic extract of A. adstringens against oral pathogens such as Streptococcus mutans, Porphyromonas gingivalis, Aggregatibacter actinomycetemcomitans, Candida albicans, and Candida dubliniensis, using microdilution (MIC) and agar diffusion methods (MBC), and the antiproliferative activity evaluating total growth inhibition (TGI) by staining the protein content with sulforhodamine B (SRB), using nine human cancer cell lines. Crude extract (CE) of A. adstringens showed some degree of activity against one or more of the strains with a MIC from 0.125 mg/mL to 63 mg/mL and MBC from 1.6 to 6.3 mg/mL and cytotoxic activity, particularly against NCI-ADR/RES, (a)n ovarian cell line expressing multiple resistance drugs phenotype. The CE is a complex mixture of possible multitarget metabolites that could be responsible for both antimicrobial and antiproliferative activities, and further investigation is required to elucidate the identity of active compounds. Nevertheless the CE itself is useful in the development of new antimicrobial treatment based on natural products to prevent oral diseases and as alternative natural source for cancer treatment and prevention2015PROMEP, Mexic

Proposal of an Alternative to the AMA Guidelines for the Evaluation of the Cervical ROM

The cervical spine is one of the most frequently injured joints in a car accident. References for the range of motion (ROM) that should be expected in a person are needed to stage the injuries. The two main objectives of this paper are to clinically validate a measuring device for the cervical spine, and to assess if the use of different ROM reference values will render different results from the American Medical Association (AMA) ROM guidelines. The present study is divided into 2 phases, a validation phase with 55 subjects and a case-control phase with 80 subjects. A BTS (Bioengineering Technology and System) system and the EBI-5 (estudio biomecánico integral) system were used for the present investigation. The intraclass correlation agreement value between both measuring devices is considered very good with a Cronbach alpha up to 0.9 in every dimension. Correlations (r) between variables are very high, not showing any values lower than 0.887. All comparisons between using AMA ROM guidelines or normative values presented significant differences (p < 0.05). The EBI-5 system has exhibited good accuracy being paired to a photogrammetric system. The use of guidelines adjusted to age constitute an alternative to the use of the AMA cervical ROM guidelines. Professionals should use age-normalized guidelines as an alternative to the AMA guidelines