USDA CSREES\u27 Role in Broadening Support for an Aging Nation

The aging of America is of major public concern and has far-reaching implications for the nation and for rural communities. In particular, older Americans in rural areas face a wide array of challenges that negatively affect their health and quality of life. Working with its land-grant university and the Cooperative Extension System partners, USDA CSREES is strategically directed and uniquely positioned to address many of these challenges through effective research, education, and Extension activities

Two Isoforms of the mRNA Binding Protein IGF2BP2 Are Generated by Alternative Translational Initiation

IGF2BP2 is a member of a family of mRNA binding proteins that, collectively, have been shown to bind to several different mRNAs in mammalian cells, including one of the mRNAs encoding insulin-like growth factor-2. Polymorphisms in the Igf2bp2 gene are associated with risk of developing type 2 diabetes, but detailed functional characterisation of IGF2BP2 protein is lacking. By immunoblotting with C-terminally reactive antibodies we identified a novel IGF2BP2 isoform with a molecular weight of 58 kDa in both human and rodents, that is expressed at somewhat lower levels than the full-length 65 kDa protein. We demonstrated by mutagenesis that this isoform is generated by alternative translation initiation at the internal Met69. It lacks a conserved N-terminal RNA Recognition Motif (RRM) and would be predicted to differ functionally from the canonical full length isoform. We further investigated IGF2BP2 mRNA transcripts by amplification of cDNA using 5′-RACE. We identified multiple transcription start sites of the human, mouse and rat Igf2bp2 genes in a highly conserved region only 50–90 nts upstream of the major translation start site, ruling out the existence of N-terminally extended isoforms. We conclude that structural heterogeneity of IGF2BP2 protein should be taken into account when considering cellular function

The Epidemiology, Genetics and Future Management of Syndactyly

Syndactyly is a condition well documented in current literature due to it being the most common congenital hand defect, with a large aesthetic and functional significance

Comparison of modeling approaches for transistor degradation: Model card adaptations vs subcircuits

The degradation of integrated field effect transistors (FETs) is an increasingly critical effect for electronic systems and their product lifetimes. To allow reliability investigations during integrated circuit (IC) design already, multiple electronic design automation (EDA) vendors offer aging simulation capabilities based on SPICE simulations. So far, the bottleneck of aging simulations is the availability of corresponding degradation models that mimick the long-term behavior of FETs due to, for instance, Hot Carrier Injection (HCI) and Bias Temperature Instability (BTI). IC designers need reasonable models that support their particular EDA environment; foundries need to equally suppor multiple EDA environments to satisfy different customers. To define FET degradation models, subcircuits and model card adaptations are feasible approaches with individual pros and cons. We compare these approaches at example degradation models with identical direct current (DC) behavior. A simulation study with ring oscillators (ROs) shows differences in transient simulation results. By applying subcircuit models, the runtime for simulating the aged circuit worsens by 28% compared to model card adaptations at a 1000-transistor circuit

Expression und antiapoptotische Funktion des Bag-1 Proteins waehrend der Embryonalentwicklung der Maus

Available from TIB Hannover: ZA 5141(6583) / FIZ - Fachinformationszzentrum Karlsruhe / TIB - Technische InformationsbibliothekSIGLEDEGerman