Analisi dinamiche in tensioni efficaci con il codice "SCOSSA"

L’incremento di pressioni interstiziali indotto da azioni sismiche in depositi sabbio-limosi determina la progressiva riduzione delle tensioni efficaci e di conseguenza della rigidezza e della resistenza dei terreni, fino a condurre alla completa liquefazione. Un metodo sviluppato per la stima di tale incremento si basa su un parametro semi-empirico, detto ‘parametro di danno’, che consente di applicare direttamente i risultati di prove cicliche di laboratorio per predire l’incremento di pressione interstiziale generato in sito dall’azione sismica. Tale procedura, implementata all’interno di un codice di calcolo non lineare, consente di effettuare analisi monodimensionali in tensioni efficaci senza adottare sofisticati modelli costitutivi del terreno. L’applicazione ad un argine danneggiato dal terremoto emiliano del 2012 ha consentito di evidenziare fenomeni di degradazione ciclica degli strati sabbiosi profondi che non sarebbe stato possibile osservare con le tradizionali analisi in tensioni totali

Un modello semplificato per la previsione dell'incremento di pressione interstiziale indotto da carichi ciclici

Le procedure che consentono la valutazione dell’incremento di pressione interstiziale indotto da azioni sismiche in terreni sabbiosi saturi si basano, tipicamente, sui risultati di prove cicliche di laboratorio a tensione o deformazione controllata. In tali procedure la storia di carico irregolare a cui è sottoposto in terreno in sito deve essere semplificata con una azione equivalente tale che produca, su provini di terreno sottoposti a prove cicliche di laboratorio, lo stesso incremento delle pressioni interstiziali atteso nel sito. In alternativa, nel caso di propagazione monodimensionale, è possibile utilizzare un unico parametro, detto ‘parametro di danno’, per modellare gli incrementi di pressione interstiziale misurati in laboratorio. Questo parametro consente di applicare direttamente i risultati di laboratorio per predire l’incremento di pressione interstiziale generato in sito da storie irregolari di tensione o deformazione dovute all’azione sismica. Tale procedura, per la sua semplicità di applicazione, consente di effettuare analisi della risposta dinamica di depositi sabbiosi saturi senza adottare sofisticati modelli costitutivi del terreno e pertanto si presta all’implementazione in codici di calcolo che operano nel dominio del tempo

Characterization of intermediate soils by innovative in-situ testing procedures using Medusa DMT

publishedVersio

Clostridium difficile infection in Italian urban hospitals: data from 2006 through 2011

BACKGROUND: In developed countries, Clostridium difficile infection (CDI) represents an emerging threat in terms of morbidity and mortality rates. In our country limited CDI epidemiological data can be found. We have conducted a 6-year retrospective study to evaluate the incidence of CDI in Italian urban hospitals. METHODS: Stool samples tested for C. difficile toxins from January 2006 to December 2011 in 5 large hospitals in Rome, Italy, were considered in the analysis. Repeated samples taken ≤ 2 months after a positive result were excluded. RESULTS: A total of 402 CDI episodes were identified. The incidence of CDI episodes progressively increased from 0.3 in 2006 to 2.3 per 10,000 patient-days in 2011. CDI episodes mostly occurred in patients > 60 years of age (77%). The >80 year-old age class reported the highest percentage of CDI episodes on tested samples (16%). Eighty percent (80%) of CDI episodes occurred in medical wards followed by surgery (10.2%) and intensive care units (9.8%). CONCLUSIONS: A significant increasing incidence of CDI episodes over the study period was observed during the years (p<.001), particularly in the older age groups. Medical wards experienced the highest number of CDI episodes as compared to intensive care and surgical wards. The increasing rate of CDI episodes over the last six years in our country, is alarming; urgent improvements in the surveillance systems and control programs are advisable

Methodologies synthesis

This deliverable deals with the modelling and analysis of interdependencies between critical infrastructures, focussing attention on two interdependent infrastructures studied in the context of CRUTIAL: the electric power infrastructure and the information infrastructures supporting management, control and maintenance functionality. The main objectives are: 1) investigate the main challenges to be addressed for the analysis and modelling of interdependencies, 2) review the modelling methodologies and tools that can be used to address these challenges and support the evaluation of the impact of interdependencies on the dependability and resilience of the service delivered to the users, and 3) present the preliminary directions investigated so far by the CRUTIAL consortium for describing and modelling interdependencies

Glucose starvation induces cell death in K-ras-transformed cells by interfering with the hexosamine biosynthesis pathway and activating the unfolded protein response

Cancer cells, which use more glucose than normal cells and accumulate extracellular lactate even under normoxic conditions (Warburg effect), have been reported to undergo cell death under glucose deprivation, whereas normal cells remain viable. As it may be relevant to exploit the molecular mechanisms underlying this biological response to achieve new cancer therapies, in this paper we sought to identify them by using transcriptome and proteome analysis applied to an established glucoseaddicted cellular model of transformation, namely, murine NIH-3T3 fibroblasts harboring an oncogenic K-RAS gene, compared with parental cells. Noteworthy is that the analyses performed in high-and low-glucose cultures indicate that reduction of glucose availability induces, especially in transformed cells, a significant increase in the expression of several unfolded protein response (UPR) hallmark genes. We show that this response is strictly associated with transformed cell death, given that its attenuation, by reducing protein translation or by increasing cell protein folding capacity, preserves the survival of transformed cells. Such an effect is also observed by inhibiting c-Jun NH2-terminal kinase, a pro-apoptotic signaling mediator set downstream of UPR. Strikingly, addition of N-acetyl-D-glucosamine, a specific substrate for the hexosamine biosynthesis pathway (HBP), to glucose-depleted cells completely prevents transformed cell death, stressing the important role of glucose in HBP fuelling to ensure UPR attenuation and increased cell survival. Interestingly, these results have been fully recognized in a human model of breast cancer, MDA-MB-231 cells. In conclusion, we show that glucose deprivation, leading to harmful accumulation of unfolded proteins in consequence of a reduction of protein glycosylation, induces a UPR-dependent cell death mechanism. These findings may open the way for new therapeutic strategies to specifically kill glycolytic cancer cells