Ecological and geochemical impact of an underground colliery waste discharge to a river

This study investigated the impact of mine wastewater disposal to a nearby river (the Bargo River). Mean electrical conductivity (EC) increased in surface waters below the mine discharge, rising more than six times from (219.5 μS/cm) upstream to 1551 μS/cm below the waste inflow. River pH increased from 7.12 (upstream) to 8.67 (downstream). The mine discharge strongly modified the ionic composition of the river. The mean concentration of several metals in the river were increased due to the mine wastewater. Nickel increased from 1.0 μg/L (upstream) to 32 μg/L (downstream). Zinc increased from 3.5 μg/L (upstream) to 23.5 μg/L (downstream). Our study also assessed the biological uptake of pollutants by growing weeping willow (Salix babylonica) cuttings in mine wastewater and contrasting to ‘control’ cuttings grown in river water from upstream of the mine. After growing in the laboratory for several weeks, the cuttings accumulated metals, dominated by barium, strontium and lithium. Results from the study constituted one of the most detailed geochemical and ecological studies investigating the impact of the coal mine waste discharge to an Australian river. Recommendations are suggested for improved regulation of the mine discharge to reduce its wastewater environmental impact

Acute Ethanol Administration Upregulates Synaptic α4-Subunit of Neuronal Nicotinic Acetylcholine Receptors within the Nucleus Accumbens and Amygdala

Alcohol and nicotine are two of the most frequently abused drugs, with their comorbidity well described. Previous data show that chronic exposure to nicotine upregulates high-affinity nicotinic acetylcholine receptors (nAChRs) in several brain areas. Effects of ethanol on specific brain nAChR subtypes within the mesolimbic dopaminergic (DA) pathway may be a key element in the comorbidity of ethanol and nicotine. However, it is unknown how alcohol affects the abundance of these receptor proteins. In the present study, we measured the effect of acute binge ethanol on nAChR α4 subunit levels in the prefrontal cortex (PFC), nucleus accumbens (NAc), ventral tegmental area (VTA), and amygdala (Amg) by western blot analysis using a knock-in mouse line, generated with a normally functioning α4 nAChR subunit tagged with yellow fluorescent protein (YFP). We observed a robust increase in α4-YFP subunit levels in the NAc and the Amg following acute ethanol, with no changes in the PFC and VTA. To further investigate whether this upregulation was mediated by increased local mRNA transcription, we quantified mRNA levels of the Chrna4 gene using qRT-PCR. We found no effect of ethanol on α4 mRNA expression, suggesting that the upregulation of α4 protein rather occurs post-translationally. The quantitative counting of YFP immunoreactive puncta further revealed that α4-YFP protein is upregulated in presynaptic boutons of the dopaminergic axons projecting to the shell and the core regions of the NAc as well as to the basolateral amygdala (BLA), but not to the central or lateral Amg. Together, our results demonstrate that a single exposure to binge ethanol upregulates level of synaptic α4∗ nAChRs in dopaminergic inputs to the NAc and BLA. This upregulation could be linked to the functional dysregulation of dopaminergic signalling observed during the development of alcohol dependence

Contextual Fear Conditioning Alter Microglia Number and Morphology in the Rat Dorsal Hippocampus

Contextual fear conditioning is a Pavlovian conditioning paradigm capable of rapidly creating fear memories to contexts, such as rooms or chambers. Contextual fear conditioning protocols have long been utilized to evaluate how fear memories are consolidated, maintained, expressed, recalled, and extinguished within the brain. These studies have identified the lateral portion of the amygdala and the dorsal portion of the hippocampus as essential for contextual fear memory consolidation. The current study was designed to evaluate how two different contextual fear memories alter amygdala and hippocampus microglia, brain derived neurotrophic factor (BDNF), and phosphorylated cyclic-AMP response element binding (pCREB). We find rats provided with standard contextual fear conditioning to have more microglia and more cells expressing BDNF in the dentate gyrus as compared to a context only control group. Additionally, standard contextual fear conditioning altered microglia morphology to become amoeboid in shape – a common response to central nervous system insult, such as traumatic brain injury, infection, ischemia, and more. The unpaired fear conditioning procedure (whereby non-reinforced and non-overlapping auditory tones were provided at random intervals during conditioning), despite producing equivalent levels of fear as the standard procedure, did not alter microglia, BDNF or pCREB number in any dorsal hippocampus or lateral amygdala brain regions. Despite this, the unpaired fear conditioning protocol produced some alterations in microglia morphology, but less compared to rats provided with standard contextual fear conditioning. Results from this study demonstrate that contextual fear conditioning is capable of producing large alterations to dentate gyrus plasticity and microglia, whereas unpaired fear conditioning only produces minor changes to microglia morphology. These data show, for the first time, that Pavlovian fear conditioning protocols can induce similar responses as trauma, infection or other insults within the central nervous system

Energic metabolism studies in pediatric cancer patients

The aim. To estimate heat capacity (C) measurements for individual dietary support of pediatric cancer patients during chemotherapy. Material and methods. 43 cancer children are examined. Group 1: 15 children, 5 children receiving polychemotherapy, 10 children – in post-BMT period (5 of them were re-examined on clinical indications). Thus we got 21 resting heat capacity measures. Group 2 includes 14 children with cancer after completing chemotherapy. Group 3 (control group) – 14 children with gastroenterological pathology. (C) is determined by indirect calorimetry, then basal metabolic rate (BMR) is calculated by formula BMR=90%C and compared with (Cf), calculated by formula WHO (1985). The results. In group 1 the average values of BMR and Еоф considerably differ: 986,5 and 1285,9 kcal accordingly (p<0,05). In group 2 BMR value is lower than Cf, however the difference is inconsiderable. The amount of children with decreased BMR in group 1 is considerably higher than in groups 2 and 3 – 76,2%; 42,9% and 28,6% accordingly (p<0,05). On the contrary group 3 includes more children with increased BMR, than group 1 (p<0,01). The marked decrease of heat capacity (C decrease) in group 1 is mainly associated with toxic effects of polychemotherapy and BMT on metabolic processes. Conclusion. The findings justify the need to monitor the pediatric cancer patients during special treatment to estimate their heat capacity to work out individual programs of dietary support with further assessment of effectiveness and correction

Regional comparison of impacts to stream macroinvertebrates from active and inactive coal mine wastewater discharges, Sydney Basin, New South Wales, Australia

This study investigates macroinvertebrates from waterways receiving wastewater from coal mines in the Sydney Basin. Three of the coal mines were inactively mining oar and four actively mining oar during sampling. Macroinvertebrates were collected from each collieries receiving waterway upstream and downstream of all mine wastewater inflows. All the coal mines wastewater discharges are licensed and regulated by the New South Wales Environment Protection Authority (NSW EPA). Results of the study show that the coal mine wastewaters being discharged are having varying negative impacts to the receiving waterways aquatic ecosystem through macroinvertebrate biotic indices, despite whether mining is active or inactive. Biotic indices measured at active and inactive coal mines show that actively mined wastewaters are most likely causing less of an impact to the receiving waterways aquatic ecosystem than inactively mined wastewaters. All the waterways receiving untreated (inactively mining) wastewaters recorded statistical differences for all biotic indices when analysed between their upstream and downstream sample locations. This was in contrasted to the actively mined (treated wastewaters) with only one of the streams sampled recording statistical differences for all biotic indices. Results suggest that once mining ceases and the treatment of the coal mine wastewaters subsequently ceases the receiving waterways aquatic ecosystem are clearly more degraded. This is of great concern as once mining ceases so does the treatment of their wastewaters. It is recommended that the NSW EPA further investigate measures of treatment post coal mining at these mines to ensure further degradation of the receiving waterways ecosystem does not occur

Regional comparison of impacts from seven Australian coal mine wastewater discharges on downstream river sediment chemistry, Sydney Basin, New south Wales Australia

This study investigates the accumulation of licensed and regulated coal mine wastewater pollutants from seven coal mines on each mines respective receiving waterways river sediments. Results from this study shows that the coal mine wastewater pollutants are accumulating within river sediments downstream of the coal mine wastewater inflows at varying levels often greater than the ANZECC guidelines for sediment and often above reference condition sediment concentrations. This is of great concern as these pollutants will likely continue to persist in the river sediment and eventually become legacy pollutants. Coal mine wastewater discharges in New South Wales are regulated by the New South Wales Environmental Protection Authority [NSW EPA] and environmental protection of receiving waterways is implemented through Environmental Protection Licenses. Environmental Protection Licenses set discharge limits for water quality and chemical concentrations within the coal mine waste waters. Though they do not take into account river sediment concentrations. It appears water column pollution regulation at these coal mines is in fact failing to protect the environment whilst still regulated and will continue into the future post mining, licensing and regulation. Water column regulation may well be impractical in protecting the environment as it appears that water column concentrations do not portray the overall environmental impact. It is recommended that the New South Wales Environmental Protection Authority investigate these findings and continue to improve water column pollutant limits as to alleviate the continued accumulation and magnification of the contaminants

The regulation and impact of eight Australian coal mine waste water discharges on downstream river water quality : a regional comparison of active versus closed mines

Water quality of rivers that received coal mine wastes from four active and three closed mines were investigated, focusing on ecologically hazardous pollutants. Zinc and nickel concentrations were highest downstream of two closed mines, particularly from the Canyon mine that closed 20 years earlier. Coal mine wastes increased nickel concentrations in waterways by an average of 25 times. The average concentration of zinc increased below mines waste discharges from 8.6 µg/L (upstream) to 83.4 µg/L (downstream). All coal mine discharges increased river salinity. Salinity increased by more than 6 times (upstream mean 101.4–741.7 µS/cm downstream). This study provides a reminder that water pollution from coal mines is a major environmental issue for both active and closed mines. The study highlights the need for more stringent and consistent environmental regulation for all mines, including key hazardous pollutants from wastes emerging from both active and closed mines

Urban geochemical contamination of high conservation value upland swamps, Blue Mountains Australia

Upland swamps of the Blue Mountains are unique and legislatively protected peat swamp communities. This study investigated water chemistry of surface waters from seven Blue Mountains Upland Swamps (BMUS), four within urbanised catchments and three from naturally vegetated catchments. The purpose of the study was to investigate any ionic contamination from urban development. Water chemistry of non-urban BMUS was acidic (mean pH 4.7) and dilute (mean EC 26.6 μS/cm) and dominated by sodium and chloride ions with most other major ions at low concentrations, often below detection limits. In contrast, urban BMUS had higher pH (mean 6.6) and salinity (mean 153.9 μS/cm) and were dominated by calcium and bicarbonate ions. The results of this study support the hypothesis that urban concrete contamination is modifying the geochemistry of urban BMUS. Further research is required to investigate ecological implications of the contamination and also to explore measures to protect such sensitive wetlands of high conservation value from urban development.5 page(s