Effect of different dietary protein and energy levels on growth of juvenile Beluga (Huso huso)

A 2 × 4 factorial design was conducted to study the effects of dietary protein and energy levels on growth performance of juvenile beluga (Huso huso) with average weight of 49.85 ± 1.64 g. Experiments were conducted with two dietary protein levels (40 and 45%) and four dietary energy level (18.5, 19.8, 21.1 and 22.4 MJ/kg diet) by ratio P/E (18.03 to 24.14 mg/kj). Fish were fed the experimental diets for 110 days. In each of the protein levels (40 and 45%), the feed efficiency, body weight increase, Protein efficiency ratio, specific growth rate, final body weight and total feed intake of fish improved significantly as dietary energy levels increased (P≤ 0.05). Growth of fish fed high- energy diets was significantly higher than those of fish fed low- energy diets at 40 and 45% dietary protein levels (P≤ 0.05). Protein content of carcass increased when dietary protein increased, but there was no significant different (P≥ 0.05) between 40 and 45 % protein. Lipid content of fish fed diet high- energy level was significantly higher than those of fish fed low dietary energy (P≤ 0.05). So the maximum growth and weight (22.4 ± 5.3 gr) was observed in treatment, that were fed diets containing 45% protein & 22.4 mega joule crude energy. Considering that no significant difference were affected for among the diet for the parameters studied, the diet containing 40 % protein, 21 % fat and 21.1 mega Joule crude energy per kilogram diet with a P/E ratio of 20 mg protein per kilo joule from a good quality source is a suitable diet in terms of physiology and economy can be considered a suitable diet to produce maximum growth in juvenile beluga in the weight class 49 to 200g

Phosphoproteomic Analysis of Cell-Based Resistance to BRAF Inhibitor Therapy in Melanoma

The treatment of melanoma by targeted inhibition of the mutated kinase BRAF with small molecules only temporarily suppresses metastatic disease. In the face of chemical inhibition tumor plasticity, both innate and adaptive, promotes survival through the biochemical and genetic reconfiguration of cellular pathways that can engage proliferative and migratory systems. To investigate this process, high-resolution mass spectrometry was used to characterize the phosphoproteome of this transition in vitro. A simple and accurate, label-free quantitative method was used to localize and quantitate thousands of phosphorylation events. We also correlated changes in the phosphoproteome with the proteome to more accurately determine changes in the activity of regulatory kinases determined by kinase landscape profiling. The abundance of phosphopeptides with sites that function in cytoskeletal regulation, GTP/GDP exchange, protein kinase C, IGF signaling, and melanosome maturation were highly divergent after transition to a drug resistant phenotype

Triple-Negative Breast Cancer comparison with Canine Mammary Tumors from light microscopy to molecular pathology

Many similar characteristics in human and dog cancers including, spontaneous development, clinical presentation, tumor heterogeneity, disease progression, and response to standard therapies have promoted the approval of this comparative model as an alternative to mice. Breast cancer represents the second most frequent neoplasm in humans after lung cancer. Triple-negative breast cancer (TNBC) constitute around 15% of all cases of breast cancer and do not express estrogen receptor (ER), progesterone receptor (PR) or overexpress human epidermal growth factor receptor 2 (HER2). Breast cancer is the second most frequent neoplasm in sexually intact female dogs after skin cancer. The majority of canine mammary tumors (CMTs) are triple-negative. Due to the high morphological, histologic, and molecular similarities between CMT and human breast cancers (HBC), human biomarkers of HBC are also observable in cases of CMT. Promising breast cancer biomarkers in both humans and canines are cancer-associated stroma (CAS), circulating tumor cells and tumor DNA (ctDNA) ), exosomes and miRNAs, and metabolites

Tau method for the numerical solution of a fuzzy fractional kinetic model and its application to the oil palm frond as a promising source of xylose

The Oil Palm Frond (a lignocellulosic material) is a high-yielding energy crop that can be utilized as a promising source of xylose. It holds the potential as a feedstock for bioethanol production due to being free and inexpensive in terms of collection, storage and cropping practices. The aim of the paper is to calculate the concentration and yield of xylose from the acid hydrolysis of the Oil Palm Frond through a fuzzy fractional kinetic model. The approximate solution of the derived fuzzy fractional model is achieved by using a tau method based on the fuzzy operational matrix of the generalized Laguerre polynomials. The results validate the effectiveness and applicability of the proposed solution method for solving this type of fuzzy kinetic model

The ESQUIRE project: Quantum Dots as scintillation detectors

The primary goal of ESQUIRE (Experiment with Scintillating Quantum dots for Ionizing Radiation Events) is the development of a new family of scintillation detectors based on scintillating nanocrystals coupled to high-quantumefficiency solid-state detectors. These detectors will be designed for the search of neutrinoless double-beta decay (0νββ), therefore an excellent energy resolution in the region of interest for the study of 0νββ (∼2% around 3 MeV) is mandatory. One of the main advantages in this approach is the easy mass scalability, which makes ESQUIRE a competitive option for next-generation experiments. During the discussion the project goal will be presented, alongside the first optical characterization of QD samples

Detection of human metapneumovirus and respiratory syncytial virus by real-time polymerase chain reaction among hospitalized young children in Iran

Background: Acute respiratory infection plays an important role in hospitalization of children in developing countries; detection of viral causes in such infections is very important. The respiratory syncytial virus (RSV) is the most common etiological agent of viral lower respiratory tract infection in children, and human metapneumovirus (hMPV) is associated with both upper and lower respiratory tract infections among infants and children. Objectives: This study evaluated the frequency and seasonal prevalence of hMPV and RSV in hospitalized children under the age of five, who were admitted to Aliasghar children�s hospital of Iran University of Medical Sciences from March 2010 until March 2013. Patients and Methods: Nasopharyngeal or throat swabs from 158 hospitalized children with fever and respiratory distress were evaluated for RSV and hMPV RNA by the real-time polymerase chain reaction (PCR) method. Results: Among the 158 children evaluated in this study, 49 individuals (31.1) had RSV infection while nine individuals (5.7) had hMPV infection. Five (55.5) of the hMPV-infected children were male while four (44.5) were female and 27 (55.2) of the RSV-infected patients were females and 22 (44.8) were males. The RSV infections were detected in mainly one year old children. Both RSV and hMPV infections had occurred mainly during winter and spring seasons. Conclusions: Respiratory syncytial virus was the major cause of acute respiratory infection in children under one-year of age while human metapneumovirus had a low prevalence in this group. The seasonal occurrence of both viruses was the same. © 2016, Ahvaz Jundishapur University of Medical Sciences