評価がいっぱい：大学図書館と評価の仕組み

journal articl

保育サービスの現状と課題 : サービス・マーケティング理論の観点から

departmental bulletin pape

Measurement of Time-Dependent CP-Violating Asymmetries in B0→ϕKS0, K+K-KS0, and η′KS0 Decays

journal articl

隠蔽された「母」 : 堀辰雄「麦藁帽子」論

departmental bulletin pape

Base compositions of the PCR amplicons generated using primer pairs VIR982, VIR985, VIR979, and VIR988.

<p>Within each column, identical base compositions for different isolates for a particular primer pair are shown grouped by the same color. DNP (did not prime) indicates that a PCR amplicon was not generated using the specified primer pair and viral DNA. The numbers in the columns under the primer pair ID indicates the numbers of each base (A, G, C, and T) in the PCR amplicons generated from the target virus. Signature source indicates whether the base composition signatures were determined from sequence or experimentally using viral genomic DNA.</p

Analytical sensitivity of PCR primer pairs VIR982, VIR985, VIR979, and VIR988 in amplification of an extract from rabbitpox-infected rabbit blood (sample ID; 3E-day 6).

<p>Ten replicate detections were performed for each dilution and a score of 100% indicates that viral-derived amplicon was detected in each of ten replicates.</p

Alignment of Orthopoxvirus sequences showing conservation of PCR primer VIR982 within this viral family, flanking a region of species-specific variations.

<p>“Dots” in a column represent homology to the reference sequence above. The primer 5 prime sequence shows the addition of non-homlogous nucleotides to increase the Tm of the primers after the first PCR cycles.</p

PCR primers used in this study.

*<p>Position of 3′ nucleotide against reference genome: Variola major, Syria 1972, DQ437592.</p

Orthopoxvirus species/strains used in this study.

<p>Orthopoxvirus species/strains used in this study.</p