Analysis of nursing productivity in the care of patients with angina pectoris in general medical wards of a university hospital

간호학과/석사[한글] 보건 의료조직은 그 어떤 형태의 것이건 거의 기본적으로 노동집약적이므로 인력이 가장 중요한 요소이다. 따라서 인력관리는 매우 중요하다. 특히 오늘날의 병원조직에서는 1989년 이후의 전국민 개보험화이후에 급증하는 의료수요와 더불어 입원수요도 급증하고 있으나 병원의 경제적 경영은 점점 더 압박을 받고 있는 실정이다. 이러한 시점에서는 병원인력가운데 다수를 점하고 있는 병원간호인력의 생산성제고가 효율적 관리방안의 큰 과제의 하나로 대두되고 있다. 본 연구의 목적은 일 대학 종합병원 내과병동에서 협심증환자의 입원 가료시 간호사가 환자를 접촉한 횟수를 이용하여 연도별, 입원일수별로 간호생산성 변화의 추이를 파악해보고자 하였다. 연구대상은 서울시내 일 대학 종합병원 내과병동에서 1985년과 1990의 각 9월 1일부터 12월 31일 사이에 입원 가료한 협심증환자 전수였다. 자료수집기간은 1991년 10월 15일부터 11월 30일까지였다. 본 연구의 목적을 달성하기 위하여 필요한 자료는 환자의 의무기록지에서 발췌, 수집하였고 관찰법을 통하여 의무기록자료를 뒷받침하였으며, 이 자료수집을 위해 본 연구자가 작성한 조사표를 사용하였다. 주요 연구결과는 다음과 같다. 1) 연도별, 협심증 환자 간호에 있어서의 간호사 1인당 생산성 (평균 환자 접촉횟수)은 1985년 158.93회, 1990년 198.00회로써 통계학적으로 유의한 차이가 있었다(t=1.94, d. f.=78. p<.05). 따라서 1990년의 간호사 1인당 생산성은 1985년에 비하여 증가하였다. 2) 연도별, 처방 간호활동 영역별, 협심증 환자 간호에 있어서의 간호사 1인당 생산성(평균 접촉횟수)은 1985년 135.5회, 1990년 175.03회였으며 통계학적으로 유의한 차이가 있었다 (t=2.22, d. f.=78, p<.05). 따라서 1990년의 처방 간호활동 영역에 대한간호사 1 인당 생산성은 1990년이 1985년에 비하여 증가하였다. 간호활동 영역별로는 활력증상 측정과 기타의 영역만을 제외한 모든 영역에서 유의한 증가가 있었다. 즉, 검사를 위한 평균 접촉횟수가 1985년 23.15회, 1990년 33.00회로써 유의한 차이가 있었으며 (t=3.24, d. f.=78, p<.01).투약을 위한 접촉횟수는 1985년 41.5 5회,1990년 61.03회로 유의한 차이가 있었다(t=2.16, d. f.=78, p<.05). 또한 식이(t=2.86, d. f.=78, p<.01),섭취량/배설량 측정(t=3.39, d. f.=64, p<.001), 처치(t=3.39, d. f.=60, p<.001) 및 퇴원(t=8.02, d. f.=65, p<.001)등의 영역도 1990년이1985년에 비하여 유의한 차이가 있었다. 3) 연도별, 비처방 간호활동 영역별, 협심증 환자 간호에 있어서의 간호사 1인당 생산성 (평균 접촉횟수)은 1985년 24.43회, 1990년 22.98회였으며 통계학적으로 유의한 차이가 없었다. 4) 입원일수 및 연도별, 협심증 환자 간호에 있어서의 간호사 1인당 생산성(평균 접촉횟수)은 제 1일이 1985년 16.60회, 1990년 21.15회였으며 통계학적으로 유의한 차이가 있었고(t=3.36, d. f.=78, p<.01), 재 2일은 1985년 16.45회, 1990년 21.05회로써 통계학적 으로 유의한 차이가 있었다(t=2.76, d. f.=78, p<.01). 따라서 입원 제 1일과 제 2일의 간호사 1인당 생산성은 1990년이 1985년에 비하여 통계학적으로 유의하게 증가하였다고 본다. 한편 제 3일부터 제 9일까지는 1985년과 1990년간에 통계학적으로 유의한 차이가 없었다. 간호사 1인당 평균 접촉횟수가 가장 많은 날은 평균 9일의 입원기간중 제 4일로써 1985년 19.38회, 1990년 21.78회였으며 제 5일 이후에는 점점 감소하여 제 9일째에는 6.53회, 1990년 7.45회에 불과한 것으로 나타났다. 5) 입원일수 및 연도별, 처방 간호활동 영역별, 협심증 환자 간호에 있어서의 간호사1인당 생산성 (평균 접촉횟수)은 다음과 같다. 제 1일의 간호사 1인당 평균 접촉횟수는 1985년 14.23회, 1990년 17.58회로써 통계학적으로 유의한 차이가 있었고(t=2.63, d.f.=78,p<.01), 검사(t=2.03, d. f.=78,p<.01),투약(t=2.85, d.f.=59, p<.01),섭취량/배설량 측정 (t=2.71, d. f.=68,p<.01)영역 등에서 유의한 차이를 보였다. 제 2일의 간호사 1인당 평균 접촉횟수는 1985년 17.63회, 1990년 20.43회로써 통계학적으로 유의한 차이가 있었으며(t=2.01, d. f.=78, p<.05), 활력증상 측정 (t=3.38,d. f.=64. p<.001), 검사( t=1.79, d. f.=78, p<.01) ,투약( t=3.67, d. f.=51,p<.001), 식이(t=3.57, d. f.=54, p<.001) 및 섭취량/배설량 측정(t=3.30, d. f.=62, p<.01)영역등에서 통계학적으로 유의한 차이를 나타냈다. 제 3일의 간호사 1인당 평균 접촉횟수는 1985년 18.00회, 1990년 19.45회였으며 통계학적으로 유의한 차이를 보였고(t=.90, d. f.=78, p<.05). 활력증상 측정 (t=2.02, d. f.=78,p<.05), 특수검사 (t=2.22, d. f.=78, p<.05) 및 필요시(Prn)투약(t=2.06, d. f.=65,p<.05)의 영역에서 유의한 차이가 있었다. 제 4일의 간호사 1인당 평균 접촉횟수는 1985년 16.10회, 1990년 16.68회였으며 통계학적으로 유의한 차이가 없었으나 검사(t=2.63, d. f.=78, p<.01), 투약(t=2.32, d. f.=50, p<.05) 및 처치(t=2.95, d. f.=47, p<.01) 영역등에서 유의한 차이를 보였다. 제 5일의 간호사 1인당 평균 접촉횟수는 1985년 13.40회, 1990년 17.85회였고, 통계학적으로 유의하게 증가하였으며(t=2.67, d. f.=70, p<.01), 검사(t=3.68, d. f.=65,p<.001)및 섭취량/배설량 측정(t=2.30, d. f.=70, p<.001)등의 처방 간호활동 영역에서 통계학 적으로 유의한 차이가 있었다. 제 6일의 간호사 1인당 환자 접촉횟수는 1985년 10.45회, 1990년 14.33회로써 1985년에 비하여 증가하였고 통계학적으로 유의한 차이를 나타냈다(t=2.29. d. f.=02, p<.05). 간호활동 영역별로는 투약(t=2.76, d. f.=52 p<.01),섭취량, 배설량 측정(t=2.75, d. f.=62 , p<.01) 및 처치(t=2.52, d. f.=48, p<.01)영역등에서 유의한 차이가 있었다. 제 7일의 간호사 1인당 환자 접촉횟수는 1986년 8.38회, 1990년 11.15회로 증가하였으나 통계학적으로 유의한 차이가 없었고, 제 8일의 간호사 1인당 환자접촉횟수도 1985년 7.20회, 1990년 8.38회로 증가하였으나 통계학적으로 유의한 차이가 없었다. 제 9일의 간호사 1인당 환자 접촉횟수는 1985년 5.63회, 1990년 5.83회로 입원일수별로 보았을 때 가장 적은 접촉횟수를 보이고 있는데 1985년과 1990년간에 통계학적으로 유의한 차이는 보이지 않았다. 평균 9일의 입원기간중 간호사가 환자와 가장 많은 접촉을 한 날은 1985년은 제 3일로써 평균 18.00회였고, 1990년은 제 2일로써 평균 20.43회였으며, 그 이후로는 점점 접촉횟수가 감소하여 제9일의 간호사 1인당 환자 접촉횟수는 1985년 평균 5.63회, 1990년 평균 5.83회에 불과하였다. 6) 입원일수 및 연도별, 비처방 간호활동 영역의 간호사 1인당 생산성(평균 접촉횟수)는 1985년과 1990년간에 통계학적으로 유의한 차이가 나타나지 않았다. 평균 9일의 입원기간중 간호사가 환자와 가장 많은 평균 접촉횟수를 나타낸 날은 제 1일로써 1985년 4.03 회, 1990년 4.27회였고 그 이후에는 점점 감소하여 제 9일은 1985년 90회, 1990년 1.00회로 불과 1회 이하의 접촉만이 있는 것으로 나타났다. 이상의 연구결과에 의하면 협심증 환자 입원가료시 1990년의 간호사 1인당 생산성은 1985년보다 증가하였으며 이는 주로 처방 간호활동 영역에서의 생산성 증가에 기인한 것으로 나타났다. 한편 간호생산성이 낮은 재원기간에 속한 환자에게는 병원운영상 보다 생산 성을 높일 수 있는 방안, 즉 병원에서의 가정간호도입등에 대한 적극적인 제도개선이 이루어져야 하겠다. Analysis Nursing Productivity in the Care of Patients with Angina Pectoris in General Medical Wards of a University Hospital Hong, Hye Kyung Department of Nursing The Graduate School of Yonsei University (Directed by Professsr Kim,Mo Im, Dr.P.H.) The purpose of this study was to compare nursing productivity in terms of the nurse-patient contacts according to the year patients were cared for and the length of hospitalization in general medical wards of a university hospital. The subjects of this study were patients who were hospitalized for the treatment of angina pectoris from September 1 through December 31,1985 and 1990. Data were collected from October 15 to November 14, 1991. The Medical records of these patients were obtained from the recording center of a university hospital . Data for this study included 1) demographic, social and economic factors, 2)admisson related factors and 3) information obtained from a form developed by the researcher to identify the nursing productivity of nursing activity. The major results of the this study were as follows: 1. Significant differences were found in the mean scores of the nurse-patient contacts per one nurse in 1985 (158.93) and in 1990 (198.00), (t=1.94, d. f.=78.p<.05) So, nursing productivity per one nurse in 1990 was greater than in 1985 2. Significant differences were found in the average number of the nurse-patient contacts per one nurse in nursing activities based on the doctor's orders in 1985 (135.5) and in 1990 (175.03), (t=2.22,d. f.=78, p<.05), So, nursing productivity per one nurse in nursing activities based on the doctor's orders in1990 was greater than in 1985. These nursing activities included medical tests(t=3.24, d. f.=78, p<.01), medications(t=2.16, d. f.=78, p<.05), diets(t=2.86, d. f.=78,p<.01), intake/output check (t=3.39, d. f.=64, p<.001), therapies(t=3.39, d. f.=60,p<.001), and discharge (t=8.02, d. f.=65,p<.001). There were no significant differences found in vital sign checks. 3. The average number of the nurse-patient contacts per one nurse in the areas of nursing activities performed without the doctor's order were 24.43 in 1985,and 22.98 in 1990. There were no significant differences found in nursing productivity per one nurse in the areas of nursing activity performed without the doctor's order in 1985 and 1990. 4. Significant differences were found in the average number of the nurse-patient contacts per one nurse according to the length of hospitalization in 1985 (16.60) and in 1990 (21.15) on the 1st day of hospitalization, (t=3.36, d. f.=78, p<.01) and on the 2nd day of hospitalization in 1985(16.45) and in 1990(21.05), (t=2.76,d. f.= 78,p=.01). So nursing productivity per one nurse according to the length of hospitalization on the 1st and 2nd day of hospitalization in 1990 was greater than for that of 1985. 5. The findings regarding nursing productivity per one nurse according to the length of hospitalization and the doctor's orders were as follows. Significant differences were found in the average number of the nurse-patient contacts per one nurse during the 1st day's hospitalization in 1985 (14.23) and in 1990 (17.58), (t=2.63,d. f.=59,p<.01). The nursing activities included medical tests (t=2.63, d. f.=78, p<.01), medications (t=2.85, d. f.=59, p<.01), and intake/output checks(t=2.71, d. f.=68, p<.01). Significant differences were found in the average number of the nurse-patient contacts per one nurse during the 2nd day's hospitalization in 1985 (17.63) and in 1990 (20.43), (t=2.01, d. f.=78, p<.05). The nursing activities included vital sign checks (t=3.38, d. f.=64, p<.001), medical tests (t=1.79. d. f.=78.p<.01),medications (t=3.67, d. f.=51, p<.001), diets(t=3.57, d. f.=54, p<.001), and intake/output check(t=3.30, d. f.=62, p<.001). Significant differences were found in the average number of the nurse-patient contacts per one nurse during the 3rs day's hospitalization in 1985 (18.00) and in 1990 (19.45):t=.90, d. f.=78,p<.05. The nursing activities included vital sign checks(t=2.02, d. f.=78,p<.05), special medical tests(t=2.22, d. f.=78,p<.05), and PRN medications(t=2.06, d. f.=65, p<.05). No significant differences were found in the average number of the nurse-patient contacts per one nurse during the 4th day's hospitalization in 1985(16.10) and in 1990 (6.68). But significant differences were found medical tests(t=2.63. d. f.=78, p<.01), medications(t=2.32, d. f.=50, p<.05), and therapies(t=2.95,d. f.=78, p<.01) were. Significant differences were found in the average number of the nurse-patient contacts per one nurse during the 5th day's hospitalization in 1985 (13.40) and in 1990 (17.85): t=2.67,d. f.=70.p<.01. The nursing activities included medical tests(t=3.68, d. f.=65, p<.001) and intake/output check(t=2.30, d. f.=70, p<.001). Significant differences were found in the average number of the nurse-patient contacts per one nurse during the 6th day's hospitalization in 1985 (10.45) and in 1990(14.33), (t=2.29.d. f.=62,p<.05). The nursing activities included medications(t=2.76, d. f.=52,7.01), intake/output check(t=2.75, d. f.=62,p<.01), and therapies(t=2.52, d. f.=48,p<.01). No significant differences were found in the average number of the nurse-patient contacts per one nurse during the 7th, 8th, and 9th days. In regard to the average length of hospitalization in 1985. the 3rd day(18.00) had the highest average number of nurse-patient contacts per one nurse, and in1990, the 2nd day (20.43) had the highest average number of nurse-patient contacts per one nurse. After the 2nd and 3rd days the average number of the nurse-patient contacts per one nurse decreased, and on the 9th day the average number of the nurse-patient contacts per one nurse in 1985 were 5.63,and in 1990 were 5.83. 6. No significant differences were found in the average number of nurse-patient contacts per one nurse in the areas of nursing activities performed without the doctor's order according to the legnth of hospitalization in 1985 and 1990. [영문] The purpose of this study was to compare nursing productivity in terms of the nurse-patient contacts according to the year patients were cared for and the length of hospitalization in general medical wards of a university hospital. The subjects of this study were patients who were hospitalized for the treatment of angina pectoris from September 1 through December 31,1985 and 1990. Data were collected from October 15 to November 14, 1991. The Medical records of these patients were obtained from the recording center of a university hospital . Data for this study included 1) demographic, social and economic factors, 2)admisson related factors and 3) information obtained from a form developed by the researcher to identify the nursing productivity of nursing activity. The major results of the this study were as follows: 1. Significant differences were found in the mean scores of the nurse-patient contacts per one nurse in 1985 (158.93) and in 1990 (198.00), (t=1.94, d. f.=78.p<.05) So, nursing productivity per one nurse in 1990 was greater than in 1985 2. Significant differences were found in the average number of the nurse-patient contacts per one nurse in nursing activities based on the doctor's orders in 1985 (135.5) and in 1990 (175.03), (t=2.22,d. f.=78, p<.05), So, nursing productivity per one nurse in nursing activities based on the doctor's orders in1990 was greater than in 1985. These nursing activities included medical tests(t=3.24, d. f.=78, p<.01), medications(t=2.16, d. f.=78, p<.05), diets(t=2.86, d. f.=78,p<.01), intake/output check (t=3.39, d. f.=64, p<.001), therapies(t=3.39, d. f.=60,p<.001), and discharge (t=8.02, d. f.=65,p<.001). There were no significant differences found in vital sign checks. 3. The average number of the nurse-patient contacts per one nurse in the areas of nursing activities performed without the doctor's order were 24.43 in 1985,and 22.98 in 1990. There were no significant differences found in nursing productivity per one nurse in the areas of nursing activity performed without the doctor's order in 1985 and 1990. 4. Significant differences were found in the average number of the nurse-patient contacts per one nurse according to the length of hospitalization in 1985 (16.60) and in 1990 (21.15) on the 1st day of hospitalization, (t=3.36, d. f.=78, p<.01) and on the 2nd day of hospitalization in 1985(16.45) and in 1990(21.05), (t=2.76,d. f.= 78,p=.01). So nursing productivity per one nurse according to the length of hospitalization on the 1st and 2nd day of hospitalization in 1990 was greater than for that of 1985. 5. The findings regarding nursing productivity per one nurse according to the length of hospitalization and the doctor's orders were as follows. Significant differences were found in the average number of the nurse-patient contacts per one nurse during the 1st day's hospitalization in 1985 (14.23) and in 1990 (17.58), (t=2.63,d. f.=59,p<.01). The nursing activities included medical tests (t=2.63, d. f.=78, p<.01), medications (t=2.85, d. f.=59, p<.01), and intake/output checks(t=2.71, d. f.=68, p<.01). Significant differences were found in the average number of the nurse-patient contacts per one nurse during the 2nd day's hospitalization in 1985 (17.63) and in 1990 (20.43), (t=2.01, d. f.=78, p<.05). The nursing activities included vital sign checks (t=3.38, d. f.=64, p<.001), medical tests (t=1.79. d. f.=78.p<.01),medications (t=3.67, d. f.=51, p<.001), diets(t=3.57, d. f.=54, p<.001), and intake/output check(t=3.30, d. f.=62, p<.001). Significant differences were found in the average number of the nurse-patient contacts per one nurse during the 3rs day's hospitalization in 1985 (18.00) and in 1990 (19.45):t=.90, d. f.=78,p<.05. The nursing activities included vital sign checks(t=2.02, d. f.=78,p<.05), special medical tests(t=2.22, d. f.=78,p<.05), and PRN medications(t=2.06, d. f.=65, p<.05). No significant differences were found in the average number of the nurse-patient contacts per one nurse during the 4th day's hospitalization in 1985(16.10) and in 1990 (6.68). But significant differences were found medical tests(t=2.63. d. f.=78, p<.01), medications(t=2.32, d. f.=50, p<.05), and therapies(t=2.95,d. f.=78, p<.01) were. Significant differences were found in the average number of the nurse-patient contacts per one nurse during the 5th day's hospitalization in 1985 (13.40) and in 1990 (17.85): t=2.67,d. f.=70.p<.01. The nursing activities included medical tests(t=3.68, d. f.=65, p<.001) and intake/output check(t=2.30, d. f.=70, p<.001). Significant differences were found in the average number of the nurse-patient contacts per one nurse during the 6th day's hospitalization in 1985 (10.45) and in 1990(14.33), (t=2.29.d. f.=62,p<.05). The nursing activities included medications(t=2.76, d. f.=52,7.01), intake/output check(t=2.75, d. f.=62,p<.01), and therapies(t=2.52, d. f.=48,p<.01). No significant differences were found in the average number of the nurse-patient contacts per one nurse during the 7th, 8th, and 9th days. In regard to the average length of hospitalization in 1985. the 3rd day(18.00) had the highest average number of nurse-patient contacts per one nurse, and in1990, the 2nd day (20.43) had the highest average number of nurse-patient contacts per one nurse. After the 2nd and 3rd days the average number of the nurse-patient contacts per one nurse decreased, and on the 9th day the average number of the nurse-patient contacts per one nurse in 1985 were 5.63,and in 1990 were 5.83. 6. No significant differences were found in the average number of nurse-patient contacts per one nurse in the areas of nursing activities performed without the doctor's order according to the legnth of hospitalization in 1985 and 1990.restrictio

누에 핵다각체병 바이러스의 p10 프로모터를 이용한 Baculovirus 발현벡터 제작

Thesis (master`s)--서울대학교 대학원 :농생물학과 곤충학전공,1999.Maste

사람 메산지움 세포에서 산화 LDL에 의해 유도되는 PAI-1 유전자 발현에 관여하는 ERK MAP kinase와 TGF-ß 신호 전달계의 상호 작용

Thesis(doctor`s)--서울대학교 대학원 :의학과 병리학 전공,2006.Docto

Antimicrobial susceptibility and plasmid profile of Neisseria gonorrhoeae

보건학과/석사[한글] 임균의 감염빈도는 정확히 알 수는 얼지만, 개발도상국에서는 아직도 많은 환자가 발생하고 있으며, penicillinase 생성균주 (penicillinase-producing Neisseria gonorrhoeae, PPNG)의 출현으로 penicillin은 임관 감염의 경험적 치료에 사용하기는 어렵게 되었다. 최근 특수직업여성의 surveillance culture에서 분리된 임균을 디스크 확산법으로 감수성을 시험하였던 바, 아직까지는 내성보고가 없던 ciprofloxacin 과 ceftriaxone에 대해 일부 균주가 감수성이 덜한 것으로 관찰되어, 이의 규명이 필요하였다. PPNG는 분리된 나라에 따라서 β-lactamase plasmid의 종류가 다르게 보고되고 있는데, 우리나라에서는 4.4-MD plasmid만 보고되었다. 이 연구에서는 1993년 특수직업여성에서 분리된 임균을 대상으로 β-lactamase 생성시험과 한천희석법을 이용하여 ceftriaxone, ciprofloxacin 등의 항균제에 대한 감수성을 시험하였고, 국내에서 분리되는 임균의 역학적인 특성을 밝히고저, PPNG에 대한 plasmid DNA 양상을 관찰하여 다음과 같은 결과를 얻었다. 1. 시험된 임균 225주 중 160주 (71%)가 β-lactamase 양성이었다. 2. Penicillin G에 감수성인 균주는 없었고, β-lactamase 음성인 균주도 58%는 penicillin G에 내성이었다. 3. Tetracycline에는 모든 균주가 내성이었고, 그 중 2%는 고도내성주였다. 4. Macrolide에 내성인 균주가 non-PPNG 중에는 얼었으나, PPNG 중의 17%가 dirithromycin에 내성이었다. 5. 디스크법으로 ceftriaxone에 감수성이 덜한 균주도 희석법으로는 모든 균주가 감수성이었으나, ciprofloxacin에 대해서는 27%의 균주가 감수성이 덜 하였다. 6. PPNG의 β-lactamase plasmid는 4.4-MD와 3.05-MD으로 추정되는 것을 각각 44%와 55%의 균주가 갖고 있었다. 이 연구에서 우리나라에서 분리되는 임균 중에는 PPNG가 맡고, 이들은 4.4-MD plasmid와 3.05-MD으로 추정되는 plasmid를 갖고 있으며, ciprofloxacin에 대한 감수성이 덜한 균주의 비율이 점차 증가하고 있어서 이에 대한 지속적인 감수성 조사가 필요하다는 결론을 얻었다. [영문] Gonococcal infections are still prevalent in developing countries, though the accurate incidence are unknown. Because of the isolation of penicillinase-producing Nesseria gonorrhoeae (PPNG) stains, it became difficult to use penicillin for the empirical treatment of gonococcal infection. It was noted that N. gonorrhoeae strains isolated for surveillance from prostitutes were less susceptible to ceftriaxone and ciprofloxacin by disk diffusion test. Upto the present such isolates have not been reported, requiring the confirmation. Plasmid profiles of PPNG were different depending on the isolated countries. In Korea, only 4.4-MD plasmids were reported. In this study, strains isolated fron prostitutes in 1993 were tested far the β-lactamase production and the susceptibilities to ceftriaxone, ciprofloxacin and others, by an agar dilution test. The plasmid pro51e of PPNGs were determined by electrophoresis. 1. Number of β-lactamase-producing strains were 160 (71%) among the 225 tested. 2. No isolates were susceptible to penicillin G, and among the non-PPNG strains 58% were resistant. 3. All of the isolates were resistant to tetracycline and the proportion of high level resistant strains were 2%. 4. Among the non-PPNGs, no isolates were resistant to macrolide, and 17% of PPNGs were resistant to dirithromycin. 5. All of the isolates were susceptible to ceftriaxone by an agar dilution, regardless of the penicillinase production, while 27% were less susceptible to ciprofloxacin. 6. PPNGs were found to carry either previously known 4.4-MD or new one with approximately 3.05-MD in size. In conclusion, PPNGs are very prevalent in our country and the strains carry 4.4-MD and suspected 3.05-MD plasmids, and the strains less susceptible to ciprofloxacin, and it is needed to monitor the pattern of susceptibilities periodically.restrictio

당화된 알부민이 사람 메산지움 세포의 TGF-β₁mRNA 발현에 미치는 영향에 관한 연구

학위논문(석사)--서울대학교 대학원 :의학과 병리학전공,1999.Maste

N102S Mutation of UBIAD1 Gene in a Family with Schnyder Crystalline Corneal Dystrophy

Purpose: Schnyder crystalline corneal dystrophy (SCCD) is an autosomal dominant disease characterized by progressive central corneal opacification and premature development of peripheral arcus in the cornea. This disease results from a point mutation of UBIAD1 in chromosome 1p34-36. Until now, 15 different mutations of UBIAD1 gene on chromosome 1p34-36 have been reported for Schnyder crystalline corneal dystrophy. More point mutations are expected to be added to the list in the future. Schnyder crystalline corneal dystrophy is a rare disease, with only three reported cases in Korea, although there has been no report of a genetically confirmed case of the disease. Case summary: We encountered six patients with an N102S mutation of UBIAD1, who are from a family of two generation with 12 family members. Genetic confirmation for Schnyder crystalline corneal dystrophy was performed on these patients. This was the first report of a genetic confirmation of Schnyder crystalline corneal dystrophy in Korea. We will discuss our cases along with a review of the related literatureope

Inhibitory Effect of Tranilast on Transforming Growth Factor-Beta-Induced Protein in Granular Corneal Dystrophy Type 2 Corneal Fibroblasts

PURPOSE: To investigate the effects of tranilast, an inhibitor of chemical mediators and fibroblast proliferation, on the expression of transforming growth factor-beta (TGF-β)-induced protein (TGFBIp) in wild-type (WT) and homozygous (HO) granular corneal dystrophy type 2 corneal fibroblasts. METHODS: Cell proliferation and cytotoxicity were measured by Cell Counting Kit-8 and lactate dehydrogenase assay. Western blotting and real-time polymerase chain reaction were used to determine changes in the expression of TGFBIp and TGFBI mRNA. We determined the effects of tranilast on phosphorylated Smad2 (pSmad2) and pSmad3, wound-healing, and expression of alpha-smooth muscle actin (α-SMA), type I collagen, and integrins. RESULTS: High concentrations of tranilast decreased proliferation of corneal fibroblasts but did not cause elevation of lactate dehydrogenase, except at 1.0 mM tranilast. TGF-β increased the expression of TGFBIp and TGFBI mRNA in WT and HO corneal fibroblasts. Cotreatment of corneal fibroblasts with tranilast and TGF-β reduced the levels of TGFBIp and TGFBI mRNA. In addition, application of tranilast reduced pSmad2 in WT and HO corneal fibroblasts and pSmad3 in HO corneal fibroblasts, both of which were increased initially by TGF-β. Tranilast delayed wound healing and reduced the expression of α-SMA, type I collagen, and some of integrins in WT and HO corneal fibroblasts. CONCLUSIONS: Application of tranilast in WT and HO corneal fibroblasts inhibited the expression of TGFBIp by blocking TGF-β signaling. Thus, tranilast may be useful in delaying or preventing the recurrence of corneal opacity in TGFBI-linked corneal dystrophies if clinical studies confirm these findings.ope