골유도 재생술과 임프란트 식립을 이용한 상악 전치부의 수복

Successful implants should fulfill both functional and esthetic needs of patients. Especially on maxillary anterior region, many surgeons undergo difficult procedures because of improper quantity and quality of soft and hard tissue. To overcome these problems, regenerative procedures such as guided bone regeneration(GBR) and ridge spreading are often used. In this study, 2 implants were installed on maxillary anterior region using ridge spreading and GBR procedure. Before implantation, osteotomes were used to spread and compact alveolar bone. After insertion of implants, allograft and alloplast were grafted on buccal concavity, and surgical site were closed using non-resorbable membrane. There were no complications during surgery and the result was satisfyingope

GC/MS 기반 대사체학 기법을 이용한 인삼 종자의 활력 연구

학위논문 (석사)-- 서울대학교 대학원 : 약학과, 2015. 2. 박정일.인삼 (Panax ginseng)은 정상 작물과는 달리 채종할 당시 배의 발달이 미숙하며, 적정한 발아 조건을 갖추었어도 일정기간의 저온감응을 받아야 발아가 가능한 3중 휴면성 특성을 가지고 있다. 때문에 종자를 널리 보급하는 데는 상당한 노력과 시간이 필요하다. 종자의 수명이 다하면 종자가 발아 능력을 잃게 되므로, 종자의 활력은 연구하는 것은 종자의 수명을 예측하는데 도움이 될 것이라 사료된다. 본 연구에서는 인삼 종자가 활력을 잃었을 때 특정 발아인자에 대응하여 생기는 종자 속 대사체의 발현 변화를 metabolomics 기반 연구를 통해 알아보고자 하였다. 이를 위하여 종자를 가혹 조건이 처리되지 않은 정상 종자와 인위적인 조건으로 노화시킨 가혹 종자로 실험군을 나누었다. 정상 종자와 노화된 종자의 활력을 검증한 뒤 두 그룹에 대표적인 발아인자인 gibberellic acid (GA3)을 농도별로 처리하였다. GC-MS를 이용하여 두 그룹간의 대사체의 차이를 분석하였고 기존에 설립된 internal library를 이용하여 다양한 내인성대사체들을 putative하게 동정하였다. 이들의 유의성을 확인하기 위하여 t-test 및 fold change analysis를 실행하였으며 다변량 통계분석을 통해 경향성을 확인하였다. 통계 분석을 통해 선별된 대사체들을 표준품을 사용하여 2차적으로 동정하였다. 정상 종자에서는 GA3의 농도가 증가할수록 몇 가지 당류 및 당알코올과 아미노산류 등이 유의성 있게 감소하였다. 반면에, 활력이 강한 정상 종자 그룹에서 증가된 대사체도 있었다. 이와 같은 변화는 노화된 종자에서는 나타나지 않았다. 선별된 대사체들은 활력이 있는 종자와 없는 종자를 구분하여 종자의 활력을 예측하는 하나의 지표의 예로써 제시될 수 있는 가능성이 있다. 본 실험을 통하여 밝혀진 활력의 유무에 따른 인삼 종자의 특정 대사체의 변화는 앞으로의 인삼 종자 연구에 발판이 될 것으로 기대된다.목 차 국문초록 ⅰ 목 차 ⅲ List of Figures ⅳ List of Tables ⅴ Ⅰ. 서 론 1 Ⅱ. 실험 3 1. 시험 재료 3 2. 시약 및 기기 3 2-1. 시약 및 용매 3 2-2. 실험 기구 4 2-3. 분석 기기 4 3. 실험방법 6 3-1. 종자 노화 및 생장 호르몬 처리 6 3-2. 종자 활력 검사 8 3-3. 종자 전처리 및 Derivatization 9 3-4. GC/MS를 이용한 종자 분석 11 3-5. 데이터 전처리와 통계 분석 13 Ⅲ. 결과 및 고찰 15 1. 활력 검사 15 2. 종자의 GC/MS 분석 결과 16 3. Principal Component Analysis (PCA) 19 4. 두 그룹간의 대사체 비교 분석 21 4-1 대사체 증감 22 4-2 관련 pathway 24 Ⅳ. 결론 28 Ⅴ. 참고문헌 29 Abstract 31Maste

심한 골흡수가 진행된 상악에서의 상악동 거상술을 동반한 임프란트 동시 식립

The sinus floor elevation procedure has been used to be one of the predictable treatments for rehabilitation of atrophic and pneumatized edentulous posterior maxilla. Sinus elevation with simultaneous implant placement could be an optimal procedure due to reduction in surgical procedures and to patient’s convenience. This study reports the successful results of sinus elevation with the sand blasted, large grit, acid-etched (SLA) surfaced implant(ITI dental imiplant system, StraumannⓇ, Basel, Switzerland) placement simultaneously. In the first case, the height of residual ridge was 2~4mm. Autogeneous bone from ramus and alloplast(Macroporous biphasic calcium phosphate-MBCP, BiomatlanteⓇ Sarl, Nantes, France) were applied after sinus elevation through lateral window approach. SLA surfaced implants(ITI) were installed simultaneously. Result after 17 months was showed successfully. In the second case, the height of residual ridge was 3~5mm. Allograft(Freeze dried bone allograft-FDBA, Oragraft, Lifenet health, Virginia Beach, VA, USA) and alloplast(MBCP) were used for sinus graft. All procedures were followed as in the first case. Result after 9 months was showed successfully. In conclusion, this study presented successful results, when sinus elevation through window approach with mixed graft material was performed with simultaneous SLA surfaced implant placement on severe atrophied maxillary alveolar ridge, minimizing patient discomfort.ope

재생 술식을 동반한 발치 후 즉시 임프란트 식립술

The placement of implants into fresh extraction socket provides many advantages to conventional delayed implant placement. It reduces total treatment time and surgical procedures with placement of the implant in an ideal position. However, it also has disadvantages such as creating a gap defect between the implant and alveolar bone and difficulty in primary closure of the surgical site. Therefore, need of a regenerative procedure is crucial in immediately placed sites for successful functional and esthetical outcomes. The current case report presents two cases with immediately placed implants performed in anterior and posterior region. A careful tooth extraction was performed to avoid any surgical trauma in the alveolar bone, and implants were successfully installed in both cases. Regenerative procedures such as guided bone regeneration and bone graft were performed in conjunction to fill the gap defect and compensate future ridge alterations. Both cases showed clinically successful results with functionally and esthetically high patient satisfaction. Immediate implant placement in conjunction with an appropriate regenerative procedure could provide successful outcome.ope

Histologic evaluation of biphasic calcium phosphate in maxillary sinus augmentation

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Guided bone regeneration on a single tooth with horizontal and vertical bone loss

The presence of adequate bone width and height is an important requisite for the proper placement of dental implants according to the concept of restoration-driven implant. Guided bone regeneration(GBR) technique has been widely used to correct ridge deformities. Although the use of non-resorbable e-PTFE membrane in GBR leads to a larger amount of bone formation the membrane can be prematurely exposed and infection can occur. In these cases, GBR were performed with or without implant using the non-absorbable membranes. There were wide fenestrations of soft tissue and membranes were exposed, which are possibly associated with the patients’heavy smoking. Dressing with chlorhexidine and medication was done for 4-5 weeks before membrane removal. Even though the quality and quantity of the regenerated bone was not as great as expected, it provided sufficient initial stability and support for the implants. In conclusion, GBR is an effective treatment option for correcting horizontal and vertical ridge deformities. The prevention and proper management of complications can lead to improved results of GBR.ope

Bone formation of newly developed biphasic calcium phosphate in rabbit calvarial defect model : A pilot study

PURPOSE: Biphasic calcium phosphates have been of great interest recently. Mixing adequate ratios of hydroxyapatite(HA) and beta-tricalcium phosphate(beta-TCP) allowed to control the resorption rate without distorting its osteoconductive property. This study evaluated the bone formation effect of newly developed biphasic calcium phosphate(BCP) in calvarial defect of rabbits. MATERIALS AND METHODS: 6 male New Zealand rabbits were used. Four defects with 8mm in diameter were created on each animal. BCP with HA/beta-TCP ratio of 7:3 and particle size of 0.5~1.0 mm was used as the test group and bovine bone with 0.25~1.0 mm particle size, as the control group. Both test and control group materials were randomly implanted in the calvarial defects and were covered witha polymer membrane. The animals were sacrificed after 12, 24, and 48 weeks of implantation under general euthanasia. Resin blocks were obtained and were stained by masson's trichrome for histological observation. RESULTS: Overall results were uneventful without any defect exposure or inflammation. The amount of new bone formation and bone maturity increased with increase in healing period at both groups. New bone in test group was mostly formed along the material particle surrounded by osteoblasts, and observation of osteoblastic stream was also present. Bone maturity increased as it was closer to thedefect margins. Under the same healing period, the test group showed more bone formation than the control group with more stable bovine bone particles remaining even after 48 weeks, whereas considerable resorption took place in BCP. Almost total defect closure was observed in test group with new bone formation in the central part of the defect. However, limited new bone formation was observed in the control group. CONCLUSION: Within the limits of the study, the present study reveals the newly developed BCP to be a good osteoconductive material. However, further studies are needed to be conducted in a different study model with a larger sample size.ope

The effect of biphasic calcium phosphate on healing of surgically created circumferential gap defect in dog model

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Implantation with various guided bone regeneration techniques

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A hybrid technique for sinus floor elevation in the severely resorbed posterior maxilla

PURPOSE: This study aimed to evaluate the effectiveness of the modified sinus floor elevation technique described hereafter as a "hybrid technique," in 11 patients with severely resorbed posterior maxillae. METHODS: Eleven patients who received 22 implants in the maxillary premolar and molar areas by the hybrid technique were enrolled in this study. A slot-shaped osteotomy for access was prepared on the lateral wall along the lower border of the sinus floor. The Schneiderian membrane was fully reflected through the lateral slot. Following drilling with the membrane protected by a periosteal elevator, the bone was grafted. All implants were placed simultaneously with sinus augmentation. The cumulative success rate was calculated and clinical parameters were recorded. Radiographic measurements were performed. RESULTS: All implants were well maintained at last follow up (cumulative success rate=100%). The mean residual bone height, augmented bone height, crown-to-implant ratio, and marginal bone loss were 4.1+/-1.64 mm, 8.76+/-1.77 mm, 1.21+/-0.33 mm, and 0.34+/-0.72 mm, respectively. CONCLUSIONS: Simultaneous implant placement with sinus augmentation by hybrid technique showed successful clinical results over a 2-year observation period and may be a reliable modality for reconstruction of a severely resorbed posterior maxillaope