14 research outputs found
Detection of human papillomavirus in neoplasms of upper aerodigestive tract and lung with its correlation to p53 mutation
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ผ๋ฌธ(๋ฐ์ฌ)--์์ธ๋ํ๊ต ๋ํ์ :์ํ๊ณผ ๋ณ๋ฆฌํ์ ๊ณต,1996.Docto
๊ฒฝ์ ์๊ธฐ๋ก ์ธํ IMF ๊ด๋ฆฌ์ฒด์ ํ์์์ ํ๊ตญ์ ์ฝ์ฐ์ ์ ์ฐ๊ตฌ๊ฐ๋ฐ ๋ฐ ๊ธฐ์ ํ๋ ฅ์ถ์ด์ ๊ดํ ์ฐ๊ตฌ
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ผ๋ฌธ(์์ฌ)--์์ธ๋ํ๊ต ๋ณด๊ฑด๋ํ์ :๋ณด๊ฑดํ๊ณผ ๋ณด๊ฑด์ฌํํ์ ๊ณต,1999.Maste
์ฌ๋๊ตฌ๊ฐ์ํผ์ธํฌ์์ ์ฒ์์ฐ๊ด ์๋ฌ๋ฅด๊ฒ์ ์ํ ์ํ ์นด์ธ์ ๋ฐํ
Dept. of Dental Science/๋ฐ์ฌAsthma is a chronic inflammatory disease of the airway characterized by variable airflow obstruction and bronchial hyper-responsiveness. In addition, patients with asthma have been reported that high prevalence of dental caries and gingivitis, which are induced by low salivation due to the medication for asthma. However, it is not known that asthma related allergens such as house dust mite (HDM) and German cockroach extract (GCE) have direct effects on the generation of gingival inflammation. In the present study, I investigated the level of interlukin-8 (IL-8) mRNA expression and the characteristics of Ca2+ signal by GCE and HDM in the human gingival epithelial cells.HDM and GCE induced increases in IL-8 mRNA expression level and intracellular concentration of Ca2+ ([Ca2+]i), respectively. Endotoxin-free GCE activated protease-activated receptor (PAR) type2, but endotoxin-free HDM did not activate any PARs. In human gingival epithelial cells, whereas lipopolisacaride, a Gram-negative endotoxin, did not induce Ca2+ signal, HDM and GCE containing endotoxins induced Ca2+ signal from thapsigargin (Tg), an inhibitor of sarco/endoplasmic reticulum Ca2+ ATPase, -sensitive Ca2+ stores via phospholipase C (PLC)/inositol 1, 4, 5-trisphosphate (IP3) pathway. These results suggest that asthma related allergens induce Ca2+ signaling and cytokine release in human gingival epithelial cells.restrictio
ๆฐ็ณๅจๆไปฃ์ ้ๅค๏คฒๅ์ ๋ํ์ฌ
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ผ๋ฌธ(์์ฌ)--์์ธ๋ํ๊ต ๋ํ์ :๊ณ ๊ณ ๋ฏธ์ ์ฌํ๊ณผ ๊ณ ๊ณ ํ์ ๊ณต,2005.Maste
๊ฐ์์ ํธ๋ฅด๋ชฌ๊ณผ ํํผ์ฑ์ฅ์ธ์, ํคํ๋ฆฐ๊ฒฐํฉ ํํผ์ฑ์ฅ์ธ์๊ฐ ์น์ฃผ์ธ๋์ธํฌ์ ๋ฏธ์น๋ ์ํฅ
Dept. of Dental Science/์์ฌ[ํ๊ธ]
์์ ํ๊ตฌ ๋ ์น์์ ์ํ๋ ์ด์์๋ ์น์ฃผ์ธ๋์ธํฌ์ ์ํฅ์ ๋ฐ์ผ๋ฉฐ, ์ธ์์ผ๋ก ์์ ํ๊ตฌ ๋ ์น์๋ฅผ ์ ์ฅํ๋ ๋ณด๊ด์ก์ ์ฌ์ ์ฑ๊ณต์ ์ํ์ ์ค์ํ ์์์ด๋ค. ๋ฐ๋ผ์, ์ต๊ทผ์ ์น์ฃผ์ธ๋์ธํฌ์ ์์กด์ ์ฆ๊ฐ์ํค๊ธฐ ์ํ ์ ์ฒ๋ฆฌ์ ๋ก์ ์ฑ์ฅ์ธ์์ ํธ๋ฅด๋ชฌ์ ๋ํ ์ฐ๊ตฌ๊ฐ ์งํ๋๊ณ ์๋ค. ์ด๋ค ์ค ๊ฐ์์ ํธ๋ฅด๋ชฌ (์ดํ T3)๊ณผ ํํผ์ฑ์ฅ์ธ์ (์ดํ EGF)๋ฐ ํคํ๋ฆฐ๊ฒฐํฉ ํํผ์ฑ์ฅ์ธ์ (์ดํ HB-EGF)๊ฐ ์ฌ๋ฌ ๊ธฐ๊ด์ ์ธํฌ ์ฆ์์ ํ์ฑํ ์ํจ๋ค๋ ๋ง์ ๋ณด๊ณ ๊ฐ ์๋ค. ์ด ์ฐ๊ตฌ์ ๋ชฉ์ ์ ๊ฐ์์ ํธ๋ฅด๋ชฌ, ํํผ์ฑ์ฅ์ธ์, ํคํ๋ฆฐ๊ฒฐํฉ ํํผ์ฑ์ฅ์ธ์๊ฐ ์ธํฌ์ ์ฆ์์ ๋ฏธ์น๋ ์ํฅ์ ์ดํด๋ณด๋ ๊ฒ์ด๋ฉฐ, ์ด ์ํธ ์์ฉ์ด ์ฌ์ ๋ชจ์ธํฌ์ ์ฆ์์ ๋ฏธ์น๋ ์ํฅ์ ์์๋ณด๋ ๊ฒ์ด๋ค. ๋ํ, ์ฑ์ฅ์ธ์๋ค์ ์กฐํฉ์ด ํํผ์ฑ์ฅ์ธ์ ์์ฉ์ฒด์ ๋ฐํ์ ๋ฏธ์น๋ ์ํฅ์ ์์๋ณด๊ณ ์ ํ๋ค.
์คํ๊ฒฐ๊ณผ T3์ EGF๋ฐ HB-EGF๋ก ์ฒ๋ฆฌํ ๊ฒฝ์ฐ, ์ต๋ ์ฆ์์ ๋ณด์ด๋ ๋๋๊น์ง๋ ๋๋์ ๋น๋กํ์ฌ ์ฆ๊ฐํ์๋ค. ์ฌ์ ๋ชจ์ธํฌ์ ์ต๋ ์ฑ์ฅ์ ๋ณด์ด๋ T3์ ๋๋๋ 10 nM์ด์์ผ๋ฉฐ, EGF๋ 10ng/ml, HB-EGF๋ 10 nM ์ด์๋ค. ์ฃผ๋ชฉํ ๊ฒ์ ํนํ 10 nM์ HB-EGF๋ฅผ ์ฒ๋ฆฌํ ๊ฒฝ์ฐ, ์น์ฃผ์ธ๋ ์ฌ์ ๋ชจ์ธํฌ์ ์ฆ์์ด ๊ฐ์ฅ ์ปธ๋ค.
T3์ EGF๋ฐ HB-EGF์ ์กฐํฉํ์ฌ ์ฒ๋ฆฌํ ๊ฒฝ์ฐ ํ๊ฐ์ง ์ฑ์ฅ์ธ์๋ง์ ์ฒ๋ฆฌํ์ ๋๋ณด๋ค ์ธํฌ ์ฆ์์ ์์ด์ ๋ณด๋ค ํจ๊ณผ์ ์ด์๋ค. ํนํ T3์ HB-EGF์ ์กฐํฉ์ ์ฌ์ ๋ชจ์ธํฌ ์ฆ์์ ์ ์ํ๊ฒ ํฅ์์์ผฐ๋ค. RT-PCR (reverse transcriptase polymer chain reaction)๋ก ํํผ์ฑ์ฅ์ธ์ ์์ฉ์ฒด(EGFR)์ ๋ฐํ์จ์ ๊ด์ฐฐํ ๊ฒฐ๊ณผ, T3๊ฐ EGFR์ ๋ฐํ์ ์ฆ๊ฐ์ํด์ ์ ์ ์์๋ค. ๋ฐ๋ผ์ T3์ ํํผ์ฑ์ฅ์ธ์์ ๋ํ ๋ฐํ ์ฆ์ ์์ฉ๊ณผ ์ด์ ๊ฒฐํฉํ๋ HB-EGF์ ์์น์์ฉ์ผ๋ก ์ด๋ค์ ์กฐํฉํ ๊ฒฝ์ฐ, ์ธํฌ ์ฆ์์ด ์ฆ๊ฐ๋์์์ ์ ์ถํ ์ ์์๋ค.
์ด๋ฒ ์ฐ๊ตฌ๋ฅผ ํตํด HB-EGF๋ฅผ ๋จ๋
์ฌ์ฉํ ๊ฒฝ์ฐ์ T3์ HB-EGF๋ฅผ ์กฐํฉํ ๊ฒฝ์ฐ๊ฐ ํจ๊ณผ์ ์ผ๋ก ์ฌ์ ๋ชจ์ธํฌ์ ์ฆ์์ ํฅ์์ํค๊ณ , ์์ ํ๊ตฌ ๋ ์น์์ ์ ์ฒ๋ฆฌ์ ๋ก์ ์ ์ฉํ๋ค๋ ๊ฒฐ๋ก ์ ๋ด๋ฆด ์ ์์๋ค.
[์๋ฌธ]Viable cells of periodontal ligament would be an important factor for the successful replantation of an avulsed tooth. Therefore, it is critical to choose the storage medium for the preservation of traumatically avulsed teeth.
Growth factors and hormones could be considered for the therapeutic application of the maintenance of viable periodontal ligament fibroblasts(PDLFs). The positive effects of tri-iodothyronine(T3), epidermal growth factor(EGF) and heparine-binding epidermal-like growth factor(HB-EGF) on other tissues have been studied previously. Therefore, T3, EGF and HB-EGF were examined to evaluate the therapeutic application on avulsed teeth.
The cell proliferation of PDLFs was increased dose-dependently in the presence of T3, EGF and HB-EGF. Maximum cellular growth was shown at the concentration of 10 nM T3, 10 ng/ml EGF and 10 nM HB-EGF. Interestingly, HB-EGF showed maximum effect on the proliferation of PDLFs, and it was suggesting that HB-EGF might be a choice of candidate for a therapeutic application.
Combined effects of these factors on the proliferation of PDLFs were also studied. Combination of T3, EGF and HB-EGF enhanced cellular growth than that of a single growth factor showing synergistic effect.
Especially, combination of T3 and HB-EGF enhanced the proliferation of PDLFs with statistical significance. I studied the effect of T3 on endogenous EGF receptor using RT-PCR. The result was that T3 enhanced the expression of EGF receptors of PDLFs.
From this study, it is suggested that HB-EGF or the combination of T3 and HB-EGF could be used as a pretreatment agent for traumatically avulsed teeth.ope