8 research outputs found
(The) effect of 13-Cis-Rentinoic acid on experimentally induced comedones of rabbits
์ํ๊ณผ/์์ฌ[ํ๊ธ]
13-cis-retinoic acid๋ vitamin A์ ํฉ์ฑ ์ ๋์ฒด๋ก์ ์ข์ฐฝ, ํนํ ์ข
๋์ ์ฌ๋ฌ ์น๋ฃ๋ฐฉ๋ฒ์ ์ํด ์ ์น๋ฃ๋์ง ์์๋ ๋ญํฌ์ฑ ๋๋ ์ํผ์ฑ ์ข์ฐฝ์ ์น๋ฃ์ ๊ทธ ์ฐ์ํ ํจ๊ณผ๊ฐ ์์์ด ์ฆ๋ช
๋์๋ค (Peck๋ฑ, 1979 ; Farrelle๋ฑ, 1980 ; Plewig ๋ฐ Wagner, 1981 ; 1982). ์ด
์ฝ๋ฌผ์ ์์ฉ ๊ธฐ์ ์ ์ ์๋ ค์ ธ ์์ง ์์ผ๋ ํผ์ง์ ์ต์ ์์ฉ (Peck๋ฑ, 1978 ; Strauss๋ฑ, 1980 ; Gomez ๋ฐ Moskowitz , 1980). ํญ์ผ์ฆ์์ฉ(Pltewig ๋ฐ Wagner, 1981 ; Plewig๋ฑ, 1982), gํญ๊ท ์์ฉ(Plewig๋ฑ, 1982), ์ด์๊ฐํ (abnormalkeratinization)์ ๋ํ ์์ฉ (Plewig๋ฑ, 1981, 1982) ๋ฑ์ด ์๋ ค์ ธ ์๋ค.
์ด์ 13-cis-retinoic acid์ ์ด์ ๊ฐํ์ ๋ํ ์์ฉ์ ๊ด์ฐฐํ๊ณ ์ , ์คํ์ ์ผ๋ก ๋ฉดํฌ (comedo)๋ฅผ ์ ๋ฐ์์ผ ์ด ์ฝ๋ฌผ์ ํจ๊ณผ๋ฅผ Planimeter, ๊ดํํ๋ฏธ๊ฒฝ ๋ฐ ์ฃผ์ฌ์ ์ํ๋ฏธ๊ฒฝ์ ์ฌ์ฉํ์ฌ ๊ด์ฐฐํ์๋ค.
๋ฉดํฌ๋ฅผ ์ ๋ฐ์ํค๊ธฐ ์ํ์ฌ ์ ์ฑ์ ์ญ์ (insoluble cutting oil)์ ๋งค์ผ 1ํ 2์ฃผ๊ฐ ๋ํฌํ์์ผ๋ฉฐ, ๋ฉดํฌํ์ฑ ํ ๋์กฐ๊ตฐ , 13-cis-retinoic acid 2ใ/ใ/day ํฌ์ฌ๊ตฐ ๋ฐ 20ใ/ใ/day ํฌ์ฌ๊ตฐ์ผ๋ก ๋๋์ด ์คํํ์๋ค. ๋์กฐ๊ตฐ์ ์ฝฉ๊ธฐ๋ฆ 2ใ๋ง์, ๊ทธ๋ฆฌ๊ณ 13-cis-retinoic aci
d ํฌ์ฌ๊ตฐ์ 13-cis-retinoic acid๋ฅผ ๊ฐ๊ฐ 2ใ/ใ, 20ใ/ใTlr ์ฝฉ๊ธฐ๋ฆ์ ๋
น์ฌ ๋งค์ผ 1ํ 4์ฃผ๊ฐ ๊ฒฝ๊ตฌํฌ์ฌํ์๋ค.
13-cis-retinoic acid์ ๋ฉดํฌ์ ๋ํ ํจ๊ณผ๋ ๋ฉดํฌ์ ๋์ด๋ณํ๋ฅผ planimeter๋ก ์ธก์ ํ๊ณ , ๋ฉดํฌ์ ํํ๋ณํ๋ฅผ ๊ดํํ๋ฏธ๊ฒฝ๊ณผ ์ฃผ์ฌ์ ์ํ๋ฏธ๊ฒฝ์ผ๋ก ๊ด์ฐฐํ์์ผ๋ฉฐ, ๊ทธ ๊ฒฐ๊ณผ๋ ๋ค์๊ณผ ๊ฐ๋ค.
1. ์ ์ฑ์ ์ญ์ ๋ํฌ 2์ฃผํ ๋ชจ๋ ์คํ๋๋ฌผ์์ ๋ฉดํฌ๊ฐ ํ์ฑ๋จ์ ์ก์์ ์ผ๋ก ๊ด์ฐฐํ ๊ตฌ ์์์ผ๋ฉฐ, ๊ดํํ๋ฏธ๊ฒฝ์ ์๊ฒฌ์ ํํผ ๋ฐ ์งํผ์ ๋นํ, ๋ชจ๋ญ์ํผ์ ์ฆ์, ํผ์ง์ ์ ํ์ ํ ์์ถ์ ๊ด์ฐฐํ ์ ์์์ผ๋ฉฐ, ๋ชจ๋ญ๊ด์ ํ์ ํ ํฝ๋๋์๊ณ , ๊ด์ ๋๋ ์์ฉ๋์ด ํํ๋ก
๋ฐ์ง๋์ด์๋ ๊ฐ์ง์ธํฌ๋ก ์ฐจ์์์ ๋ณผ ์ ์์๋ค. ๋ฉดํฌ์ ๋์ด๋ ์ ์ฑ์ ์ญ์ ๋ํฌ์ ์ ๋นํ์ฌ 10๋ฐฐ ๊ฐ๋ ์ฆ๊ฐ๋์๋ค. ๊ทธ๋ฆฌ๊ณ ์ฃผ์ฌ์ ์ํ๋ฏธ๊ฒฝ์ ์๊ฒฌ์ ๋ฉดํฌ๋ ๊ฐ์ง์ธํฌ๋ค์ด ๋ชจ์ฌ ๋ง์น ๊ตญํ๊ฝ๊ณผ ๊ฐ์ ํํ๋ฅผ ์ด๋ฃจ๊ณ ์์๋ค.
2. ๊ดํํ๋ฏธ๊ฒฝ์ ์๊ฒฌ์ ๋ฉดํฌํ์ฑ 4์ฃผํ ๋์กฐ๊ตฐ์ ๋นํ์ฌ 13-cis-retinoic acid 20ใ/ใ/day ํฌ์ฌ๊ตฐ์์ ํฝ๋๋์๋ ๋ชจ๋ญ๊ด์ ๊ธธ์ด ๋ฐ ํญ์ด ํ์ ํ ๊ฐ์๋์์ผ๋ฉฐ, ๊ทธ ์์ ๋น๊ต์ ๋์จํ ์๋์ ๊ฐ์ง์ธํฌ๋ก ์ฐจ์์์ผ๋, ํผ์ง์ ํฌ๊ธฐ์ ์ฆ๊ฐ๋ ๋์ผํ์์์ ๊ด์ฐฐํ ์ ์์๊ณ , 2ใ/ใ/day ์ฌ๊ตฐ์์๋ ๋๋ ทํ ์กฐ์งํ ์ฐจ์ด๊ฐ ์์๋ค.
3. ๋ฉดํฌ์ ๋์ด๋ ๋ฉดํฌํ์ฑ 4์ฃผํ ๋์กฐ๊ตฐ๊ณผ 13-cis-retinoic acid 2ใ/ใ/day ํฌ์ฌ๊ตฐ ๊ฐ์๋ ์์์๋ ์ฐจ์ด๊ฐ ์์์ผ๋, ์ด ๋๊ตฐ๊ณผ 13-cis-retinoic acid 2ใ/ใ/day ํฌ์ฌ๊ตฐ ์ฌ์ด์์๋ ํต๊ณํ์ ์ผ๋ก ์์์๋ ์ฐจ์ด๊ฐ ์์๋ค.
4. ์ฃผ์ฌ์ ์ํ๋ฏธ๊ฒฝ์ ์๊ฒฌ์ ๋ฉดํฌํ์ฑ 4์ฃผํ ๋์กฐ๊ตฐ์์ ๋ฉดํฌ๋ ๊ฐ์ง์ธํฌ๋ค์ด ๋น๊ต์ ๋จ๋จํ ๊ฒฐํฉํ์ฌ ๋ถ๋ถ์ ์ผ๋ก ๊ตญํ๊ฝ๋ชจ์์ ์ด๋ฃจ๊ณ ์์์ผ๋, 13-cis-retinoic acid 2ใ/ใ/day ํฌ์ฌ๊ตฐ์์๋ ๊ตญํ๊ฝ๋ชจ์์ ๋ฉดํฌ๋ฅผ ๊ฑฐ์ ๊ด์ฐฐํ ์ ์์๊ณ ๋์กฐ๊ตฐ์ ๋นํด ๋ฉดํฌ์
์ง๊ฒฝ์๋ ์ฐจ์ด๊ฐ ์์์ผ๋ ๊ทธ ๋์ด๋ ํ์ ํ ๊ฐ์๋จ์ ๋ณผ ์ ์์๋ค. 13-cis-retinoic acid 2ใ/ใ/day ํฌ์ฌ๊ตฐ์์๋ ๋ฉดํฌ๊ฐ ๊ตญํ๊ฝ๋ชจ์์ ์์ ํ ์์คํ์์ผ๋ฉฐ, ๋์กฐ๊ตฐ์ ๋นํด ์ง๊ฒฝ ๋ฐ ๋์ด๊ฐ ํ์ ํ ๊ฐ์๋์ด, ๋ชจ๋ญ์ฃผ์์ ๊ฐ์ง์ธํฌ๋ค์ด ํ๋ฝ๋์ด ๊ฑฐ์น ํํผํ๋ฉด์ ์ด
๋ฃจ๊ณ ์๋ ์๊ฒฌ์ ์ ์ธํ๊ณ ๋ ์ ์ฑ์ ์ญ์ ๋ํฌ์ ์๊ฒฌ๊ณผ ๊ฑฐ์ ์ ์ฌํจ์ ๊ด์ฐฐํ ์ ์์๋ค. ์ด์๊ณผ ๊ฐ์ ์๊ฒฌ์ผ๋ก ๋ณด์, ์ ์ฑ์ ์ญ์ ์ ์ํด ์ ๋ฐ๋ ๋ฉดํฌ์ ๋ํ์ฌ 13-cis-retinoic
acid์ ์ด์๊ฐํ์ ๋ํ ์์ฉ์ด ์์์ ๊ด์ฐฐํ ์ ์์์ผ๋ฉฐ, ์ด๋ ์๋ง๋ VAA ๊ตญ์๋ํฌ์์์ ๊ฐ์ด ๊ฐ์ง์ธํฌ์ฌ์ด์ ๊ฒฐํฉ๋ ฅ์ ๊ฐ์์ํด์ผ๋ก์ ๋ํ๋์ง ์๋ ์ถ์ธก๋๋ฉฐ, ๊ทธ ์์ฉํจ๊ณผ๋ ์ฝ๋ฌผํฌ์ฌ๋๊ณผ ๊ด๊ณ๊ฐ ์์์ ๊ด์ฐฐํ ์ ์๋ค.
The Effect of 13-Cis-Retinoic Acid on Experimentally Induced Comedones of Rabbits
Byoung Moon Choi
Department of Medical Science The Graduate School Yonsei University
(Directed by Professor Sungnack Lee, M.d)
The effect of 13-cis-retinoic acid, which is clinically Very effective in the
treatment of severe acne, on experimentally induced comedones was studied by means
of histology, planimetry, and scanning electron microscope.
To induce comedo formation, the insoluble cutting oil was applied for 2 weeks to
the ears of adult male albino rabbits just external to the ear canal. After comedo
formation, 13-cis-retinoic acid was fed by feeding tube to rabbits daily for 4
weeks. A low dose of 2ใ/ใ and a high dose of 20ใ/ใ body weight were selected.
Soy-bean oil served as the vehicle.
The results are summarized as follows:
1. The topical application of insoluble cutting oil caused a marked follicular
accentuation in all the experimental animals at 2 weeks. Microscopically,
thickening of the epidermis, proliferation of the epithelium of the follicles and
sebaceous gland ducts, diminution of the sebaceous gland acini, and dermal
thickening were evident. The follicular lumen was greatly distended by very compact
and adherent horny cells. The scanning electron microscope clearly showed the
comedo projecting as a chrysanthemum flower-like structure.
2. Planimetrically, the high dose of 13-cis-retinoic acid led to a significant
decrease in size of comedones in comparison to the soy-bean oil control group and
the group treated with the low dose of 13-cis-retinoic acid for 4 weeks (P <
0.001).
3. In the group treated with high dose of 13-cis-retinoic acid. the size of
follicle was drastically reduced and the lumen contained a small amount of loose,
non-adherent horny cells ; while the group treated with low dose of 13-cis-retinoic
acid showed similar histological changes as the soy-bean oil control.
4. In scanning electron microscopic study of the group treated with high dose of
13-cis-retinoic acid, the comedo was no longer a compact chrysanthemum flower like
structure and its component cells were only loosely adherent. In the group treated
with low dose of 13-cis-retinoic acid, the height of comedones was markedly
decreased, but the diameter wasn't decreased in comparison to the soy-bean oil
control .
From these findings, it is clear that the action of oral 13-cis-retinoic acrid on
experimental comedones induced by insoluble cutting oil is the reduction of
abnormal keratinization, probably resulting from the decreased cohesiveness between
horny cells as seen in the topical vitamin A acrid application. The therapeutic
effect of 13-cis-retinoic acid is dose-dependent.
[์๋ฌธ]
The effect of 13-cis-retinoic acid, which is clinically Very effective in the treatment of severe acne, on experimentally induced comedones was studied by means of histology, planimetry, and scanning electron microscope.
To induce comedo formation, the insoluble cutting oil was applied for 2 weeks to the ears of adult male albino rabbits just external to the ear canal. After comedo formation, 13-cis-retinoic acid was fed by feeding tube to rabbits daily for 4
weeks. A low dose of 2ใ/ใ and a high dose of 20ใ/ใ body weight were selected.
Soy-bean oil served as the vehicle.
The results are summarized as follows:
1. The topical application of insoluble cutting oil caused a marked follicular accentuation in all the experimental animals at 2 weeks. Microscopically, thickening of the epidermis, proliferation of the epithelium of the follicles and
sebaceous gland ducts, diminution of the sebaceous gland acini, and dermal thickening were evident. The follicular lumen was greatly distended by very compact and adherent horny cells. The scanning electron microscope clearly showed the comedo projecting as a chrysanthemum flower-like structure.
2. Planimetrically, the high dose of 13-cis-retinoic acid led to a significant decrease in size of comedones in comparison to the soy-bean oil control group and the group treated with the low dose of 13-cis-retinoic acid for 4 weeks (P < 0.001).
3. In the group treated with high dose of 13-cis-retinoic acid. the size of follicle was drastically reduced and the lumen contained a small amount of loose, non-adherent horny cells ; while the group treated with low dose of 13-cis-retinoic
acid showed similar histological changes as the soy-bean oil control.
4. In scanning electron microscopic study of the group treated with high dose of 13-cis-retinoic acid, the comedo was no longer a compact chrysanthemum flower like structure and its component cells were only loosely adherent. In the group treated with low dose of 13-cis-retinoic acid, the height of comedones was markedly decreased, but the diameter wasn't decreased in comparison to the soy-bean oil control .
From these findings, it is clear that the action of oral 13-cis-retinoic acrid on experimental comedones induced by insoluble cutting oil is the reduction of abnormal keratinization, probably resulting from the decreased cohesiveness between horny cells as seen in the topical vitamin A acrid application. The therapeutic
effect of 13-cis-retinoic acid is dose-dependent.restrictio
ํ๊ตญ ์ฒญ์ฅ๋ ์์์ ์ต์ ํ๋ฐ๋๊ณผ ํผ๋ถ์์ ์๊ด๊ด๊ณ์ ๊ดํ ์ฐ๊ตฌ
์ํ๊ณผ/๋ฐ์ฌ[์๋ฌธ]
[ํ๊ธ]
์ต์ ํ๋ฐ๋์ ์์ธ์ ์ ์ด์ฉํ ์ง๋จ, ์น๋ฃ ๋ฑ์ ๊ธฐ๋ณธ์ ์ด๋ฉฐ ํ์์ ์ผ๋ก ์ธก์ ๋์ด์ผ ํ
๋ ์์ธ์ ์กฐ์ฌ์ฉ๋์ผ๋ก, ์ธก์ ์์ ๋ฐ๋ผ ๊ทธ ํ์ ๊ธฐ์ค์ด ๋ค๋ฅด๊ณ , ์์ธ์ ์กฐ์ฌ ํ ์ผ์ ์๊ฐ
์ ๋ฐ๋์ ์ต์ ํ๋ฐ๋์ ํ๋
ํด์ผ ํ๋ ์๊ฐ์ ์ธ ์ ์ฝ๊ณผ ๊ณผ๋ํ ์์ธ์ ์กฐ์ฌ๋ถ์์ ์์ด
๊ฐ, ์ํฌ ๋ฑ์ ๋ถ์์ฉ์ด ๋ฐ์ํ ์ ์๋ ๋จ์ ์ด ์๋ค.
์ต์ ํ๋ฐ๋์ ์ธก์ ํ๋๋ฐ ์๊ธฐํ ๋ฐ์ ๊ฐ์ ๋จ์ ๋ค์ด ์์ด ์์์ ์ผ๋ก ๋ถํธํ ์ ์ด ๋ง
๊ธฐ ๋๋ฌธ์, ๋ณธ ์ฐ๊ตฌ์์๋ ํ๊ตญ์ธ ๋จ๋
์ฒญ์ฅ๋
137๋ช
์ ๋ฐฐ๋ถ์์ ์๋๊ณ๋ก ํผ๋ถ์์ ์ธก์
ํ์ฌ Hunter's L, a, b system์ผ๋ก ํ์ํ๊ณ , UV-B๋ฅผ ์กฐ์ฌํ์ฌ ์ต์ ํ๋ฐ๋์ ์ธก์ ํ ํ,
๋จ๊ณ๋ณ ์คํ๊ท๋ถ์ (stepwise multiple regression)์ ์ค์ํ์ฌ, ์ต์ ํ๋ฐ๋๊ณผ ํผ๋ถ์
์ ์๊ด๊ด๊ณ๋ฅผ ๊ณ ์ฐฐํ๋ฏ๋ก์ ๋ค์๊ณผ ๊ฐ์ ๊ฒฐ๊ณผ๋ฅผ ์ป์๋ค.
1. ์ฐ๋ น๊ฐ์ ํผ๋ถ์์ 20๋๋ณด๋ค 40๋์ ํฉ์๋(b๊ฐ)๊ฐ ๋์ ๊ฒ์ ์ ์ธํ๊ณ ๋ฐ๊ธฐ (L๊ฐ)
์ ์ ์๋(a๊ฐ)์์ ํต๊ณํ์ ์ผ๋ก ์ ์ํ ์ฐจ์ด๋ฅผ ๋ณด์ด์ง ์์๋ค. ๋ ์ฐ๋ น์ธต๊ฐ์ ์์ฐจ๋ 0
.9N.B.S.๋จ์๋ก์ ๊ทผ์ํ์๋ค.
2. ๋จ๋
๊ฐ์ ํผ๋ถ์์ L๊ฐ, a๊ฐ ๋ฐ b๊ฐ ๋ชจ๋์์ ํต๊ณํ์ ์ผ๋ก ์ ์ํ ์ฐจ์ด๋ฅผ ๋ณด์๋ค(P
<0.05). ์ฆ, ๋จ์์ ํผ๋ถ์์ ์ฌ์๋ณด๋ค ๊ฒ๊ณ , ์ ์๋๋ ๋์์ผ๋ฉฐ ํฉ์๋๋ ๋ฎ์๋ค. ๋จ๋
๊ฐ์ ์์ฐจ๋ 5.7 N.B.S.๋จ์๋ก์ ๊ฐ์งํ ์ ์๋ ์ ๋์๋ค.
3. ์ต์ ํ๋ฐ๋์ 20๋์ 40๋๊ฐ์๋ ์ ์ํ ์ฐจ์ด๊ฐ ์์์ผ๋, ๋จ๋
๊ฐ์๋ ์ ์ํ ์ฐจ์ด
๊ฐ ์์ด ๋จ์์ ์ต์ ํ๋ฐ๋์ด ์ฌ์๋ณด๋ค ๋์๋ค(P<0.05).
4. ๋จ๊ณ๋ณ ์คํ๊ท๋ถ์ ๊ฒฐ๊ณผ, ์ต์ ํ๋ฐ๋๊ณผ ํผ๋ถ์์ ์๋ก ๋ฐ์ ํ ์๊ด๊ด๊ณ๊ฐ ์์๋ค.
ํผ๋ถ์์ ๊ฒฐ์ ํ๋ ํผ๋ถ์์์ค ๋ฉ๋ผ๋์ด ์ต์ ํ๋ฐ๋์ ๊ฒฐ์ ํ๋ ๊ฐ์ฅ ์ค์ํ ์ธ์์์ผ๋ฉฐ
, ๋ฉ๋ผ๋ ์ด์ธ์ ํผ๋ถ์์(carotenoids, ์ฐํ ๋ฐ ํ์ ํค๋ชจ๊ธ๋ก๋น)์ ๊ต์์ฌ์ ์ ์ํ ์ฐ
๋ ๋ฑ๋ ์ต์ ํ๋ฐ๋์ ์ํฅ์ ๋ฏธ์นจ์ ๊ด์ฐฐํ ์ ์์๋ค.
5. ๋ณธ ์ฐ๊ตฌ ๊ฒฐ๊ณผ, ๋ค์๊ณผ ๊ฐ์ ์คํ๊ท ๋ฐฉ์ ์์ ๊ตฌํ ์ ์์์ผ๋ฉฐ, ์ด ๋ฐฉ์ ์์ผ๋ก ์ต์
ํ๋ฐ๋์ 43.6% ์ค๋ช
ํ ์ ์์๋ค.
์ต์ ํ๋ฐ๋=216.8-8.2Xl-4.7X2-2.4X3-1.5X4
Xl=์ฑ๋ณ ; ๋จ์:1, ์ฌ์=0 X2=์ฐ๋ น ; 20๋ =0, 40๋ =1
X3=L๊ฐ(ํผ๋ถ์์ ๋ฐ๊ธฐ) X4=b๊ฐ(ํผ๋ถ์์ ํฉ์๋)
The Relationship between Minimal Erythema Dose and Skin Color in Korean Adults
Byungmun Choi
Department of Medical Science The Graduate School, Yonsei University
(Directed by Professor Sungnack Lee, M.D.)
Minimal erythema dose [MED] is used basically and essentially as a guide for
determining therapeutic and diagnostic UV radiation dosage. However, there are a
number of disadvantages to using it; [1] different definitions are used by
different workers. [2] it has to be read at an exactly specified time after UV
irradiation, and [3] burning sensation, vesicles, and other side effects may
devalop at an excessively irradiated sites. Because of these, measuring the MED
clinically difficult. Therefore, with a colorimeter we measured the color of skin
on the backs of two groups of Korean adults [137 total] and described it, using
Hunter's L, a, b system. After measuring the color of skin, the MED was measured at
the same site. The relationship between MED and color of skin was analyzed, using
stepwise multiple regression.
The results obtained were as follows :
1. The color of skin between the adults in their 20s and those in their 40s did
not differ significantly, although the skin of the individuals in their 40s was
more yellow than that of the individuals in their 20s. The color difference between
the two groups was 0.9N.B.S. units and the difference in visual sensation was
slight.
2. In the sex distribution of skin color, there was a significant difference
between the sexes. The color of skin of male were lower in lightness, higher in
redness and lower in yellowness than those of female The color difference between
the sexes was 5.7 N.B.S. units and the difference in visual sensation was
appreciable.
3. In measuring the MED, there was no significant statistical difference between
the two age groups. But there was significant difference between the sexes, i.e.,
MED was higher for male than for female
4. The stepwise multiple regression analysis showed that the MED was well
correlated to the color of skin. As a results of this analysis, the major MED
determinant was melanin and other color pigments of skin [carotenoids, oxy-and
reduced hemoglobin] and scattering by collagen fiber were minor MED determinants.
5. From the results, we have formulated following multiple regression equation:
MED=216.8-8.2X1-4.7X2-2.4X3-1.5X4
X1=Sex; male=1, female=0
X2=Age; 20s=0, 40s=1
X3= Lightness of skin color
X4= Yellowness of skin colorrestrictio