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    (A) study on the attitude of the clinical nursing instructor

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    ๊ฐ„ํ˜ธํ•™๊ณผ/์„์‚ฌ[ํ•œ๊ธ€] ๊ฐ„ํ˜ธ๊ต์œก์€ ๋‹ค๋ฅธ ๊ต์œก๋ถ„์•ผ์™€๋Š” ๋‹ฌ๋ฆฌ ์ด๋ก ์˜ ๊ต์ˆ˜(teaching )์™€ ์ด๊ฒƒ์„ ์‹ค์ œ ๊ฐ„ํ˜ธ์— ์ ์šฉํ•  ์ˆ˜ ์žˆ๋Š” ๊ต์œก์ด ํ•จ๊ป˜ ํ–‰ํ•ด์ง€๊ณ  ์žˆ๋‹ค. ์ด๋ก ๊ณผ ์‹ค์ œ๊ฐ€ ๊ฒฐ์ฝ” ๋…๋ฆฝ์ ์ด๊ฑฐ๋‚˜ ์œ ๋ฆฌ๋œ ์ƒํƒœ์—์„œ๋Š” ์ด๋ฃจ์–ด ์งˆ ์ˆ˜ ์—†์œผ๋ฉฐ ์‹ค์ œ์ ์œผ๋กœ ๊ฐ„ํ˜ธํ™œ๋™๊ณผ ์ƒํ˜ธ๊ด€๊ณ„๋ก  ์ด๋ฅผ ๋•Œ ๋น„๋กœ์„œ ์ตœ๋Œ€์˜ ํšจ๊ณผ๋ฅผ ์–ป๊ฒŒ๋˜๋Š” ๊ฒƒ์ด๋‹ค. ๊ทธ๋Ÿฌ๋ฏ€๋กœ ๋ณธ ์—ฐ๊ตฌ๋Š” ๊ฐ„ํ˜ธ๊ต์œก์˜ ํ•œ ์ค‘์š”ํ•œ ๋ถ€๋ถ„์ธ ์ž„์ƒ๊ฐ„ํ˜ธ๊ต์œก์„ ๋‹ด๋‹นํ•˜๊ณ  ์žˆ๋Š” ์ž„์ƒ ์ง€๋„๊ต์ˆ˜์˜ ํšจ์œจ์ ์ธ ์ž์งˆ์„ ๊ทœ๋ช…ํ•˜๊ณ  ์ด์— ๋Œ€ํ•œ ํ•™์ƒ๋“ค์˜ ๋ฐ˜์‘์„ ์กฐ์‚ฌํ•˜์—ฌ ๊ฐ„ํ˜ธํ•™ ์ž„์ƒ ๊ต์œก๋ฐœ์ „์— ๋„์›€์ด ๋˜๊ณ ์ž ์‹œ๋„๋œ ๊ฒƒ์ด๋‹ค. ๋ณธ ์—ฐ๊ตฌ์—์„œ ์‚ฌ์šฉ๋œ ์งˆ๋ฌธ์ง€์˜ ๋‚ด์šฉ์€ ๋‹ค์Œ๊ณผ ๊ฐ™๋‹ค. ์ž„์ƒ์ง€๋„๊ต์ˆ˜์˜ ์ž์งˆ๋กœ์„œ ๊ทธ์˜ ์ž„์ƒ๊ฐ„ํ˜ธ๊ต์œก์— ๋Œ€ํ•œ ์ง€์‹, ์ค€๋น„๋„, ํ•™์ƒ์˜ ๋™๊ธฐ์œ ๋ฐœ, ์—ด์˜, ์ง‘๋‹จํ† ์˜ ์ง€๋„, ์œตํ†ต์„ฑ, ์ž์„ธ, ๊ต์œกํ™˜๊ฒฝ์˜ ์กฐ์„ฑ, ํ•™์ƒ์„ ์ดํ•ดํ•˜๋Š” ์ •๋„, ํ•™์ƒ์ง€๋„ ๋“ฑ์œผ๋กœ ๊ตฌ์„ฑํ•˜์˜€์œผ๋ฉฐ ์ด์ƒ 10๊ฐœ ํ•ญ๋ชฉ์˜ ์ž์งˆ์€ ๋‹ค์‹œ 49๊ฐœ์˜ ๋ฌธํ•ญ์œผ๋กœ ์„ธ๋ถ„ํ•˜์˜€๋‹ค. ์—ฐ๊ตฌ๋ฐฉ๋ฒ•์œผ๋กœ๋Š” Y๋Œ€ํ•™๊ต ๊ฐ„ํ˜ธ๋Œ€ํ•™ ํ•™์ƒ 115๋ช…์„ ๋Œ€์ƒ์œผ๋กœ ํ•˜์—ฌ ์งˆ๋ฌธ์ง€๋ฅผ ๋Œ๋ ค ํ‰์ •ํ•˜๊ฒŒ ํ•˜์˜€๋‹ค. ๊ฒฐ๊ณผ๋Š” ๋ฐฑ๋ถ„์œจ, ฮบ-test, T-test, ์ƒ๊ด€๊ด€๊ณ„ ๋“ฑ์œผ๋กœ ํ†ต๊ณ„ ์ฒ˜๋ฆฌํ•˜์˜€๋‹ค. ์—ฐ๊ตฌ๊ฒฐ๊ณผ๋ฅผ ์š”์•ฝํ•˜๋ฉด ๋‹ค์Œ๊ณผ ๊ฐ™๋‹ค. 1. ์ž„์ƒ์ง€๋„๊ต์ˆ˜์˜ ์ž„์ƒ๊ฐ„ํ˜ธ๊ต์œก์— ๋Œ€ํ•œ ์ง€์‹์— ๊ด€ํ•œ 3๋ฌธํ•ญ ๋ชจ๋‘์—์„œ ๊ทธ ๋ฐ˜์‘์€ ๏ผ‚๋‹ค์†Œ๊ฐ„ ์ผ์น˜ํ•œ๋‹ค๏ผ‚๊ฐ€ ๊ฐ๊ฐ ๊ฐ€์žฅ ๋†’์€ ๋น„์œจ์„ ์ฐจ์ง€ํ•˜๊ณ  ์žˆ๋‹ค. 2. ์ž„์ƒ์ง€๋„๊ต์ˆ˜์˜ ์ž„์ƒ๊ฐ„ํ˜ธ๊ต์œก์˜ ์ค€๋น„๋„์— ๊ด€ํ•œ 5๋ฌธํ•ญ ์ค‘ ๊ทธ ๋ฐ˜์‘์€ ๏ผ‚๋‹ค์†Œ๊ฐ„ ์ผ์น˜ํ•œ ๋‹ค๏ผ‚๊ฐ€ 2๋ฌธํ•ญ, ๏ผ‚๋ณดํ†ต์ •๋„ ์ด๋‹ค๏ผ‚๊ฐ€ 3๋ฌธํ•ญ์—์„œ ๊ฐ๊ฐ ๊ฐ€์žฅ ๋†’์€ ๋น„์œจ์„ ์ฐจ์ง€ํ•˜๊ณ  ์žˆ๋‹ค. 3. ํ•™์ƒ์˜ ์ž„์ƒ์‹ค์Šต์— ๋Œ€ํ•œ ๋™๊ธฐ์œ ๋ฐœ์— ๊ด€ํ•œ 3๋ฌธํ•ญ ๋ชจ๋‘์—์„œ ๊ทธ ๋ฐ˜์‘์€ ๊ฐ๊ฐ ๏ผ‚๋ณดํ†ต์ •๋„์ด๋‹ค๏ผ‚๊ฐ€ ๊ฐ€์žฅ ๋†’์€ ๋น„์œจ์„ ์ฐจ์ง€ํ•˜๊ณ  ์žˆ๋‹ค. 4. ์ž„์ƒ์ง€๋„๊ต์ˆ˜์˜ ์ž„์ƒ์‹ค์Šต์ง€๋„์—์˜ ์—ด์˜์— ๊ด€ํ•œ 5๋ฌธํ•ญ ์ค‘ ๊ทธ ๋ฐ˜์‘์€ ๏ผ‚๋‹ค์†Œ๊ฐ„ ์ผ์น˜ํ•œ๋‹ค๏ผ‚๊ฐ€ 2๋ฌธํ•ญ, ๏ผ‚๋ณดํ†ต์ •๋„์ด๋‹ค๏ผ‚๊ฐ€ 3๋ฌธํ•ญ์—์„œ ๊ฐ๊ฐ ๊ฐ€์žฅ ๋†’์€ ๋น„์œจ์„ ์ฐจ์ง€ํ•˜๊ณ  ์žˆ๋‹ค. 5. ์ž„์ƒ์ง€๋„๊ต์ˆ˜์˜ ์ž„์ƒ์‹ค์Šต๊ณผ์ • ์ค‘ ์ง‘๋‹จํ† ์˜ ์ง€๋„์— ๊ด€ํ•œ 5๋ฌธํ•ญ ์ค‘ ๊ทธ ๋ฐ˜์‘์€ ๏ผ‚๋‹ค์†Œ๊ฐ„ ์ผ์น˜ํ•œ๋‹ค๏ผ‚๊ฐ€ 2๋ฌธํ•ญ, ๏ผ‚๋ณดํ†ต์ •๋„์ด๋‹ค๏ผ‚๊ฐ€ 3๋ฌธํ•ญ์—์„œ ๊ฐ๊ฐ ๊ฐ€์žฅ ๋†’์€ ๋น„์œจ์„ ์ฐจ์ง€ํ•˜๊ณ  ์žˆ๋‹ค. 6. ์ž„์ƒ์ง€๋„๊ต์ˆ˜์˜ ์œตํ†ต์„ฑ์— ๊ด€ํ•œ 5๋ฌธํ•ญ ์ค‘ ๊ทธ ๋ฐ˜์‘์€ ๏ผ‚๋ณดํ†ต์ •๋„์ด๋‹ค๏ผ‚๊ฐ€ 4๋ฌธํ•ญ, ๏ผ‚๋‹ค์†Œ๊ฐ„ ์ผ์น˜ํ•˜์ง€ ์•Š๋Š”๋‹ค๏ผ‚๊ฐ€ 1๋ฌธํ•ญ์—์„œ ๊ฐ๊ฐ ๊ฐ€์žฅ ๋†’์€ ๋น„์œจ์„ ์ฐจ์ง€ํ•˜๊ณ  ์žˆ๋‹ค. 7. ์ž„์ƒ์ง€๋„๊ต์ˆ˜์˜ ์ž์„ธ์— ๊ด€ํ•œ 5๋ฌธํ•ญ ์ค‘ ๊ทธ ๋ฐ˜์‘์€ ๏ผ‚๋‹ค์†Œ๊ฐ„ ์ผ์น˜ํ•œ๋‹ค๏ผ‚๊ฐ€ 1๋ฌธํ•ญ, ๏ผ‚๋ณดํ†ต์ •๋„์ด๋‹ค๏ผ‚๊ฐ€ 4๋ฌธํ•ญ์—์„œ ๊ฐ๊ฐ ๊ฐ€์žฅ ๋†’์€ ๋น„์œจ์„ ์ฐจ์ง€ํ•˜๊ณ  ์žˆ๋‹ค. 8. ์ž„์ƒ์ง€๋„๊ต์ˆ˜์˜ ์ž„์ƒ๊ต์œกํ™˜๊ฒฝ์˜ ์กฐ์„ฑ์— ๊ด€ํ•œ 5๋ฌธํ•ญ ์ค‘ ๊ทธ ๋ฐ˜์‘์€ ๏ผ‚๋‹ค์†Œ๊ฐ„ ์ผ์น˜ํ•œ๋‹ค๏ผ‚๊ฐ€ 4๋ฌธํ•ญ, ๏ผ‚๋ณดํ†ต์ •๋„์ด๋‹ค๏ผ‚๊ฐ€ 1๋ฌธํ•ญ์—์„œ ๊ฐ๊ฐ ๊ฐ€์žฅ ๋†’์€ ๋น„์œจ์„ ์ฐจ์ง€ํ•˜๊ณ  ์žˆ๋‹ค. 9. ์ž„์ƒ์ง€๋„๊ต์ˆ˜๊ฐ€ ํ•™์ƒ๋“ค์„ ์ดํ•ดํ•˜๋Š” ์ •๋„์— ๊ด€ํ•œ 4๋ฌธํ•ญ ์ค‘ ๏ผ‚๋‹ค์†Œ๊ฐ„ ์ผ์น˜ํ•œ๋‹ค๏ผ‚๊ฐ€ 2๋ฌธํ•ญ, ๏ผ‚๋ณดํ†ต์ •๋„์ด๋‹ค๏ผ‚๊ฐ€ 2๋ฌธํ•ญ์—์„œ ๊ฐ๊ฐ ๊ฐ€์žฅ ๋†’์€ ๋น„์œจ์„ ์ฐจ์ง€ํ•˜๊ณ  ์žˆ๋‹ค. 10. ์ž„์ƒ์‹ค์Šต๊ณผ์ •์—์„œ์˜ ํ•™์ƒ์ง€๋„์— ๊ด€ํ•œ 8๋ฌธํ•ญ ์ค‘ ๊ทธ ๋ฐ˜์‘์€ ๏ผ‚๋‹ค์†Œ๊ฐ„ ์ผ์น˜ํ•œ๋‹ค๏ผ‚๊ฐ€ 2๋ฌธํ•ญ, ๏ผ‚๋ณดํ†ต์ •๋„์ด๋‹ค๏ผ‚๊ฐ€ 6๋ฌธํ•ญ์—์„œ ๊ฐ๊ฐ ๊ฐ€์žฅ ๋†’์€ ๋น„์œจ์„ ์ฐจ์ง€ํ•˜๊ณ  ์žˆ๋‹ค. 11. ์ž„์ƒ์ง€๋„๊ต์ˆ˜์˜ ์ž์งˆ์˜ ๊ฐ ํ•ญ๋ชฉ๋ณ„ ํ‰์ •์€ ๋ชจ๋‘ ๋ณดํ†ต๋ณด๋‹ค ๋†’์€ ์ ์ˆ˜๋ฅผ ๋‚˜ํƒ€๋‚ด๊ณ  ์žˆ๋‹ค. 12. ์ž„์ƒ์ง€๋„๊ต์ˆ˜์˜ ๊ฐ ์ž์งˆ๊ฐ„์˜ ์ƒ๊ด€์„ฑ์—์„œ๋Š” ๊ฐ ํ•ญ๋ชฉ๊ฐ„์˜ ์ƒ๊ด€์„ฑ์ด ํฐ ๊ฒƒ์œผ๋กœ ๋‚˜ํƒ€๋‚ฌ๋‹ค. ( p= 0.001 ) ์œ„์˜ ๊ฒฐ๊ณผ๋กœ ๋ณด๋ฉด ์ž„์ƒ์ง€๋„๊ต์ˆ˜๋Š” ์ž„์ƒ๊ฐ„ํ˜ธ๊ต์œก์— ๊ด€ํ•œ ์ „๋ฌธ์ ์ธ ์ง€์‹์„ ๊ฐ–์ถ”๊ณ  ์žˆ๊ณ  ์ธ๊ฐ„๊ด€๊ณ„์— ์žˆ์–ด ํ•™์ƒ์„ ์ž˜ ์ดํ•ดํ•˜๋ฉฐ ์•ˆ์ •๋œ ํ™˜๊ฒฝ์—์„œ ์ž„์ƒ์‹ค์Šต์ง€๋„๋ฅผ ํ•˜๊ณ  ์žˆ๋‹ค. ํ•œํŽธ ํ•™์ƒ๋“ค์—๊ฒŒ ์š”๊ตฌ๋˜๋Š” ๊ฒƒ์€ ํ•™์ƒ์œผ๋กœ ํ•˜์—ฌ๊ธˆ ์ž…์ƒ์‹ค์Šต์— ๋Œ€ํ•œ ์˜์š•์„ ๋ถˆ๋Ÿฌ ์ผ์œผ์ผœ์ฃผ๊ธฐ ์œ„ํ•œ ๊ฒฝํ—˜์„ ๊ฐ–๊ฒŒํ•˜๋Š” ๊ฒƒ๊ณผ ํ•™์ƒ๋“ค์˜ ์š”๊ตฌ์— ๋”ฐ๋ผ ์ž„์ƒ์‹ค์Šต ์ง€๋„์˜ ๋ฐฉํ–ฅ์„ ์œตํ†ต์„ฑ์žˆ๊ฒŒ ์กฐ์ ˆํ•˜์—ฌ ์ฃผ๋Š” ๊ฒƒ์ด์—ˆ๋‹ค. A Study on the Attitude of the Clinical Nursing Instructor Chung, Hyun Sook Department of Nursing The Graduate School Yonsei University (Directed by Professor Chung, Hyun Sook) Clinical learning experience should be given to students consistent with theory which they learned. The characteristics of clinical instructors influence on accomplishmer of students' learning experience. This study attempted to find out clinical instructors' attitudes influencing on Students' learning experience. The purposes of this study are as follows . 1. To identify the characteristics of the clinical instructor. 2. To investigate students' reaction to the characteristics of clinical instructor according to modified the Teacher Quality Evaluation Scalds developed by Yong Horn Park. 3. To investigate the correlation each one characteristic of the clinical instructor with others. The tool used in this study was modified the teacher Quality Evaluation Scalds developed by Yong Horn Park. The Questionnaire was included following ten categories. Knowledge about clinical nursing instruction degree of readiness, motivation of students, earnestness, conduct the conference, flexibility, attitudes, set up the environment for clinical instruction, degree of students understanding, instruction of students. Above mentioned characteristics of ten categories are devided into forty-nine items. The subject of the study was 115 students of College of Nursing, Y. University. Analysis of the data was carried out with percentage, ๏ผธ**2-test, T-test, and Correlation. The result of this study are as follows. 1. The response of three items concerning with the knowledge about clinical instruction of clinical instructor is ๏ผ‚almost agree๏ผ‚ with highest rate each item. 2. The response of five items concerning with the degree of readiness of clinical instructor are two items ๏ผ‚almost agree๏ผ‚ and three items ๏ผ‚neutral๏ผ‚ with highest rate. 3. The response of three items concerning with the motivation of the students is ๏ผ‚almost agree๏ผ‚ with highest rate each item. 4.The response of five items concerning with the earnestness of instruction of students are two items ๏ผ‚almost agree๏ผ‚and three items ๏ผ‚neutral๏ผ‚ with highest rate. 5. The response of five items concerning with conduct the conference of clinical instructor are two items ๏ผ‚almost agree๏ผ‚and three items ๏ผ‚neutral๏ผ‚ with highest rate. 6. The response of five items concerning with flexibility of clinical instructor are four items ๏ผ‚neutral๏ผ‚ and one item ๏ผ‚almost agree๏ผ‚ with highest rate. 7. The response of five items concerning with attitudes of clinical instructor are one items ๏ผ‚almost agree๏ผ‚and four items ๏ผ‚neutral๏ผ‚ with highest rate. 8. The response of five items concerning with set up the environment for clinical instructor are four items ๏ผ‚almost agree๏ผ‚and one items ๏ผ‚neutral๏ผ‚ with highest rate. 9. The response of four items concerning with degree of students understanding of clinical instructor are two items ๏ผ‚almost agree๏ผ‚and t์žฌ items ๏ผ‚neutral๏ผ‚ with highest rate. 10. The response of eight items concerning with instruction of students are two items ๏ผ‚almost agree๏ผ‚and six items ๏ผ‚neutral๏ผ‚ with highest rate. 11. All grades of each item concerning with the characteristics of clinical instructor appears higher then average. 12. Correlation of characteristics of clinical instructor, correlation of each category appears high. According to the above result, clinical instructors have professional knowledge about clinical instruction and understand the students very well with respect to interpersonal relation and instruct the clinical practice in stable environment. The students want to have learning experience to stimulate their desire to learn. The direction of clinical instruction should be coordinated according to students' need with flexibility. [์˜๋ฌธ] Clinical learning experience should be given to students consistent with theory which they learned. The characteristics of clinical instructors influence on accomplishmer of students' learning experience. This study attempted to find out clinical instructors' attitudes influencing on Students' learning experience. The purposes of this study are as follows . 1. To identify the characteristics of the clinical instructor. 2. To investigate students' reaction to the characteristics of clinical instructor according to modified the Teacher Quality Evaluation Scalds developed by Yong Horn Park. 3. To investigate the correlation each one characteristic of the clinical instructor with others. The tool used in this study was modified the teacher Quality Evaluation Scalds developed by Yong Horn Park. The Questionnaire was included following ten categories. Knowledge about clinical nursing instruction degree of readiness, motivation of students, earnestness, conduct the conference, flexibility, attitudes, set up the environment for clinical instruction, degree of students understanding, instruction of students. Above mentioned characteristics of ten categories are devided into forty-nine items. The subject of the study was 115 students of College of Nursing, Y. University. Analysis of the data was carried out with percentage, ๏ผธ**2-test, T-test, and Correlation. The result of this study are as follows. 1. The response of three items concerning with the knowledge about clinical instruction of clinical instructor is ๏ผ‚almost agree๏ผ‚ with highest rate each item. 2. The response of five items concerning with the degree of readiness of clinical instructor are two items ๏ผ‚almost agree๏ผ‚ and three items ๏ผ‚neutral๏ผ‚ with highest rate. 3. The response of three items concerning with the motivation of the students is ๏ผ‚almost agree๏ผ‚ with highest rate each item. 4.The response of five items concerning with the earnestness of instruction of students are two items ๏ผ‚almost agree๏ผ‚and three items ๏ผ‚neutral๏ผ‚ with highest rate. 5. The response of five items concerning with conduct the conference of clinical instructor are two items ๏ผ‚almost agree๏ผ‚and three items ๏ผ‚neutral๏ผ‚ with highest rate. 6. The response of five items concerning with flexibility of clinical instructor are four items ๏ผ‚neutral๏ผ‚ and one item ๏ผ‚almost agree๏ผ‚ with highest rate. 7. The response of five items concerning with attitudes of clinical instructor are one items ๏ผ‚almost agree๏ผ‚and four items ๏ผ‚neutral๏ผ‚ with highest rate. 8. The response of five items concerning with set up the environment for clinical instructor are four items ๏ผ‚almost agree๏ผ‚and one items ๏ผ‚neutral๏ผ‚ with highest rate. 9. The response of four items concerning with degree of students understanding of clinical instructor are two items ๏ผ‚almost agree๏ผ‚and t์žฌ items ๏ผ‚neutral๏ผ‚ with highest rate. 10. The response of eight items concerning with instruction of students are two items ๏ผ‚almost agree๏ผ‚and six items ๏ผ‚neutral๏ผ‚ with highest rate. 11. All grades of each item concerning with the characteristics of clinical instructor appears higher then average. 12. Correlation of characteristics of clinical instructor, correlation of each category appears high. According to the above result, clinical instructors have professional knowledge about clinical instruction and understand the students very well with respect to interpersonal relation and instruct the clinical practice in stable environment. The students want to have learning experience to stimulate their desire to learn. The direction of clinical instruction should be coordinated according to students' need with flexibility.restrictio

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    ํ•™์œ„๋…ผ๋ฌธ(์„์‚ฌ)--์„œ์šธ๋Œ€ํ•™๊ต ๋ณด๊ฑด๋Œ€ํ•™์› :๋ณด๊ฑดํ•™๊ณผ ๋ณด๊ฑดํ•™์ „๊ณต,2003.Maste

    (A) correlation study on nurses' subjective perception and their effectiveness

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    ์‚ฌํšŒ๊ต์œกํ–‰์ • ์ „๊ณต/์„์‚ฌ[ํ•œ๊ธ€] ๋ณธ ์—ฐ๊ตฌ๋Š” ๊ฐ„ํ˜ธ์›์˜ ํšจ์œจ์„ฑ์— ๋Œ€ํ•˜์—ฌ ๊ฐœ์ธ์˜ ์ฃผ๊ด€์ ์ธ ์ง€๊ฐ๊ณผ ์ง๋ฌดํšจ์œจ์„ฑ๊ฐ„์˜ ์ƒ๊ด€๊ด€๊ณ„๋ฅผ ์•Œ์•„๋ณด๊ธฐ ์œ„ํ•œ ์—ฐ๊ตฌ์ด๋‹ค. ํ˜„๋Œ€์˜ ๊ฐ„ํ˜ธ๋Š” ๊ทธ ๋ฒ”์œ„๊ฐ€ ํ™˜์ž์˜ ์‹ ์ฒด์  ๊ฐ„ํ˜ธ๋ฟ๋งŒ ์•„๋‹ˆ๋ผ ์งˆ๋ณ‘์˜ ์˜ˆ๋ฐฉ๊ณผ ๊ตญ๋ฏผ๋ณด๊ฑด ํ–ฅ์ƒ์— ๊ทธ ์ค‘์ ์„ ๋‘๊ณ  ์žˆ์œผ๋ฉฐ, ํ™˜์ž์˜ ์œก์ฒด์  ๊ฐ„ํ˜ธ๋Š” ๋ฌผ๋ก , ์ •์‹  ๋ฐ ์ •์„œ์ ์ธ ์ „์ธ๊ฐ„ํ˜ธ ( Comprehensive Nursing Care)๋กœ์„œ ์ค‘์š”ํ•œ ์ „๋ฌธ์ง์˜ ํ•˜๋‚˜๋กœ ์ธ์ •๋˜๊ณ  ์žˆ๋‹ค. ๊ธ‰๋ณ€ํ•˜๋Š” ์„ธ๋Œ€์˜ ์‚ฌํšŒ์  ์š”๊ตฌ์— ๋”ฐ๋ผ ๊ฐ„ํ˜ธ์›์˜ ์ˆ˜ ๊ฐ€ ๊ธ‰์ฆํ•˜๊ฒŒ๋˜๊ณ , ์–‘์  ํŒฝ์ฐฝ์œผ๋กœ ์ธํ•œ ๊ฐ„ํ˜ธ์›์˜ ์งˆ์  ํ–ฅ์ƒ๊ณผ ์ „๋ฌธ์ง์œผ๋กœ์„œ์˜ ์‚ฐํ˜ธ์—…๋ฌด๊ฐ€ ๋Šฅ๋ฅ ์ ์œผ๋กœ ์ˆ˜ํ–‰๋˜๊ณ  ์žˆ๋Š”๊ฐ€์— ๋Œ€ํ•œ ๊ตฌ์ฒด์ ์ธ ํšจ์œจ์„ฑ ์ธก์ •์˜ ์—ฐ๊ตฌ์™€ ํ‰๊ฐ€๊ฐ€ ์‹คํ—˜์„ ํ†ตํ•ด์„œ ์ด๋ฃจ์–ด์ ธ์•ผ ํ•  ๋‹จ๊ณ„์— ์ด๋ฅธ ๊ฒƒ์ด๋‹ค. ์ „๋ฌธ์ง์˜ ํšจ์œจ์„ฑ์— ๊ด€ํ•ด์„œ ๊ทธ ๋™์•ˆ ๋งŽ์ด ์—ฐ๊ตฌ๋˜์–ด ์™”์œผ๋‚˜ ํšจ์œจ์ ์ธ ์ „๋ฌธ๊ฐ€์™€ ๋น„ํšจ์œจ์ ์ธ ์ „๋ฌธ๊ฐ€๋ฅผ ๊ตฌ๋ถ„ํ•˜๋Š” ๋ฐ์— ๋Œ€ํ•œ ๋šœ๋ ทํ•œ ๊ธฐ์ค€์ด ์„ธ์›Œ์ ธ์žˆ์ง€ ์•Š์„ ๋ฟ๋งŒ ์•„๋‹ˆ๋ผ ์—ฐ๊ตฌ์ž์ฒด๊ฐ€ ์™ธํ˜•์ ์ธ ๊ฐ๊ด€์  ์ ‘๊ทผ๋ฐฉ๋ฒ•์— ์˜ํ•˜์—ฌ ์—ฐ๊ตฌ๋˜์–ด ์™”๋‹ค. ๊ทธ๋Ÿฌ๋‚˜ ๋ณธ ์—ฐ๊ตฌ๋Š” ๊ทผ๋ณธ์ ์œผ๋กœ ์ฃผ๊ด€์  ์ง€๊ฐ์— ์˜ํ•˜์—ฌ ์ธ๊ฐ„์˜ ํ–‰๋™์ด ์ง€๋ฐฐ๋œ๋‹ค๋Š” ์ง€๊ฐ์‹ฌ๋ฆฌํ•™์˜ ์ž…์žฅ์—์„œ ์ง€๊ฐ์˜ ํ–ฅ์„ฑ์„ ์ „๋ฌธ์ง์—…์ธ์šฉ "์ง€๊ฐํ–ฅ์„ฑ๊ฒ€์‚ฌ" (์ตœ์ •ํ›ˆ 1971)๋ฅผ ์‚ฌ์šฉํ•˜์—ฌ 1972๋…„ 5์›”์—์„œ 7์›” ์‚ฌ์ด์— S์ข…ํ•ฉ๋ณ‘์›์— ๊ทผ๋ฌดํ•˜๋Š” ๊ฐ„ํ˜ธ์› 100๋ช…์„ ๋Œ€์ƒ์œผ๋กœ ๊ทธ๋“ค์˜ ๊ทผ๋ฌด์„ฑ์ ๊ณผ ํ•™๋ ฅ, ๊ฒฝ๋ ฅ ๋ฐ ์—ฐ๋ น๊ณผ์˜ ์ƒ๊ด€๊ด€๊ณ„๋ฅผ ์นด์ด ์ž์Šน๋ฒ•(Chi-square rest)์œผ๋กœ ์ฒ˜๋ฆฌํ•˜์˜€๋‹ค. ๊ฒฐ๊ณผ๋Š” ๋‹ค์Œ๊ณผ ๊ฐ™๋‹ค. 1. ๊ฐ„ํ˜ธ์›์˜ ์ง€๊ฐํ–ฅ์„ฑ๊ณผ ๊ทผ๋ฌด์„ฑ์ ๊ฐ„์—๋Š” ํ†ต๊ณ„์ ์œผ๋กœ ์˜์˜์žˆ๋Š” ์ƒ๊ด€์ด ๋“ค์–ด๋‚˜์ง€ ์•Š์•˜๋‹ค. 2. ๊ฐ„ํ˜ธ์›์˜ ์ง€๊ฐํ–ฅ์„ฑ๊ณผ ํ•™๋ ฅ๊ฐ„์—๋Š” ์ •์  ์ƒ๊ด€์ด ์žˆ๋‹ค. 3. ๊ฐ„ํ˜ธ์›์˜ ์ง€๊ฐํ–ฅ์„ฑ๊ณผ ์ง๋ฌด๊ฒฝ๋ ฅ๊ฐ„์—๋Š” ํ†ต๊ณ„์ ์œผ๋กœ ์˜์˜์žˆ๋Š” ์ƒ๊ด€์ด ๋“ค์–ด๋‚˜์ง€ ์•Š์•˜๋‹ค. 4. ๊ฐ„ํ˜ธ์›์˜ ์ง€๊ฐํ–ฅ์„ฑ๊ณผ ์—ฐ๋ น๊ณ„์ธต๊ฐ„์—๋Š” ํ†ต๊ณ„์ ์œผ๋กœ ์˜์˜์žˆ๋Š” ์ƒ๊ด€์ด ๋“ค์–ด๋‚˜์ง€ ์•Š์•˜๋‹ค. 5. ๊ฐ„ํ˜ธ์›์˜ ์ง€๊ฐํ–ฅ์„ฑ๊ณผ ํ•˜์œ„์š”์ธ ์ค‘ ์ž๊ธฐ ์ž์‹ ์— ๋Œ€ํ•œ ์ง€๊ฐ๊ณผ ๊ทผ๋ฌด์„ฑ์ , ์ง๋ฌด๊ฒฝ๋ ฅ, ์—ฐ๋ น๊ณ„์ธต๊ฐ„์—๋Š” ์ƒ๊ด€์ด ์žˆ๋‹ค. 6. ๊ฐ„ํ˜ธ์›์˜ ์ง€๊ฐํ–ฅ์„ฑ์˜ ํ•˜์œ„์š”์ธ ์ค‘ ํƒ€์ธ์— ๋Œ€ํ•œ ์ง€๊ฐ๊ณผ ํ•™๋ ฅ๊ฐ„์—๋Š” ํ†ต๊ณ„์ ์œผ๋กœ ์˜์˜์žˆ๋Š” ์ƒ๊ด€์ด ๋“ค์–ด๋‚˜์ง€ ์•Š์•˜๋‹ค. ์ด ๊ฒฐ๊ณผ์— ๋”ฐ๋ผ ๋‹ค์Œ๊ณผ ๊ฐ™์€ ์•ž์œผ๋กœ์˜ ๋ฌธ์ œ์ ์„ ๋ฐœ๊ฒฌํ•˜๊ฒŒ ๋˜์—ˆ๋‹ค. 1. ๊ฐ„ํ˜ธ์›์˜ ๊ทผ๋ฌด์„ฑ์  ํ‰๊ฐ€์ฒ™๋„์˜ ์‹ ๋ขฐ๋„์™€ ํƒ€๋‹น๋„์— ๋Œ€ํ•œ ์—ฐ๊ตฌ์™€ ํ‘œ์ค€ํ™”๊ฐ€ ํ•„์š”ํ•˜๋‹ค. 2. ๊ฐ„ํ˜ธ์›์˜ ์ฃผ๊ด€์  ์ง€๊ฐํ–ฅ์„ฑ๊ฒ€์‚ฌ์™€ ๊ทผ๋ฌด์„ฑ์ ์˜ ์ž๊ธฐ ํ‰๊ฐ€๊ฐ„์˜ ์ƒ๊ด€์˜ ์ •๋„๋ฅผ ์•Œ์•„๋ณด๋Š” ์—ฐ๊ตฌ๊ฐ€ ํ•„์š”ํ•˜๋‹ค. 3. ๊ฐ„ํ˜ธ์›์— ๋Œ€ํ•œ ๊ฐ๊ด€์  ๊ทผ๋ฌด์„ฑ์  ํ‰๊ฐ€์™€ ์ฃผ๊ด€์  ์ž๊ธฐ ํ‰๊ฐ€๊ฐ„์˜ ์ƒ๊ด€์—ฐ๊ตฌ๊ฐ€ ํ•„์š”ํ•˜๋‹ค. [์˜๋ฌธ] The present study in the view of humanistic psychology was attempted to seek the purpose of study, the relationship between nurses' working efficiency that measured by an observational working efficiency scale and their subjective perception. "Perceptual Orientation Scale"(Choy, Chung Hoon 1971) was administered to 100 nurse during the period of May to july 1972, and the relationship with their ratings by their supervising head nurses. Educational background, ages, and their lengths of carrer were compared with their subjective perception. All the relationship were calculated by the method of chi-squares and correlational coefficients. The results of this study are summerized as follow: 1. There is no significant relationship between nurses' subjective perceptions and their working ratings. 2. There is significant relationship between nurses' subjective perceptions and their educational backgrounds. 3. There is no significant relationship between nurses' subjective perceptions and their lengths of carrer. 4. There is no significant relationship between nurses' subjective perceptions and their ages. The results of the present study are interpreted to the sampled research group of this study and are not to be extended to any other groups.restrictio

    (A) study on relationship between the value of self-actualization and job satisfaction of registered nurses

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    ๋ณด๊ฑดํ•™/์„์‚ฌ[ํ•œ๊ธ€] ์ „๋ฌธ์  ๊ฐ„ํ˜ธ์ˆ˜ํ–‰์— ์žˆ์–ด์„œ ๊ฐ„ํ˜ธ์›์˜ ์ž์•„์‹คํ˜„์„ฑ์ด ๊ฐ„ํ˜ธ์›์˜ ์ง๋ฌด๋งŒ์กฑ๋„์— ํฐ ๋ณ€์ธ์œผ๋กœ ์ž‘์šฉํ•œ๋‹ค๊ณ  ์ƒ๊ฐ๋˜์–ด ๊ฐ„ํ˜ธ์›์˜ ์ž์•„์‹คํ˜„์„ฑ์ •๋„์™€ ์ง๋ฌด, ๋งŒ์กฑ๋„์˜ ์ƒ๊ด€๊ด€๊ณ„๋ฅผ ํŒŒ์•…ํ•˜๊ณ ์ž ๋ณธ ์—ฐ๊ตฌ๋ฅผ ์‹œ๋„ํ•˜์˜€๋‹ค. ์—ฐ๊ตฌ๋Œ€์ƒ์€ ๋ณด๊ฑด๊ฐ„ํ˜ธ์› 104๋ช…, ์ž„์ƒ๊ฐ„ํ˜ธ์› 160๋ช…์„ ๋Œ€์ƒ์œผ๋กœ ํ•˜์˜€๋‹ค. ์—ฐ๊ตฌ๋„๊ตฌ๋กœ๋Š” ์ž์•„์‹คํ˜„์„ฑ์— ์žˆ์–ด์„œ๋Š” ๊น€์žฌ์€, ์ด๊ด‘์ž์˜ ์ž์•„์‹คํ˜„๊ฒ€์‚ฌ ์—ฐ๊ตฌ๋ชฉ์ ์— ๋งž๊ฒŒ ์ถ”์ถœํ•˜์˜€์œผ๋ฉฐ, ์ง๋ฌด๋งŒ์กฑ๋„๋Š” Brayfield์™€ Rothe๊ฐ€ ๊ฐœ๋ฐœํ•œ ๋„๊ตฌ๋ฅผ ์ด์šฉํ•˜์˜€๋‹ค. ์ž๋ฃŒ์ˆ˜์ง‘๊ธฐ๊ฐ„์€ 1984๋…„ 4์›” 1์ผ์—์„œ 4์›” 15์ผ ๊นŒ์ง€ ์ด๋‹ค. ์ž๋ฃŒ๋ถ„์„ ๋ฐฉ๋ฒ•์€ ๋ฐฑ๋ถ„์œจ, t-๊ฒ€์ •, x**2-๊ฒ€์ •, ๋ถ„์‚ฐ๋ถ„์„, ํ”ผ์–ด์Šจ ์ƒ๊ด€๊ณ„์ˆ˜, ํŽธ์ƒ๊ด€๊ณ„์ˆ˜ ๋ถ„์„ ๋ฐฉ๋ฒ•์„ ์‚ฌ์šฉํ•˜์˜€๋‹ค. ์—ฐ๊ตฌ์˜ ๊ฒฐ๊ณผ๋ฅผ ์š”์•ฝํ•˜๋ฉด ๋‹ค์Œ๊ณผ ๊ฐ™๋‹ค. 1. ์ผ๋ฐ˜์  ํŠน์„ฑ๊ณผ ์ž์•„์‹คํ˜„์„ฑ์ •๋„์™€์˜ ๊ด€๊ณ„์—์„œ๋Š” ์—ฐ๋ น. ๊ฒฐํ˜ผ์—ฌ๋ถ€, ์ข…๊ต์œ ๋ฌด, ๊ทผ๋ฌด๋…„ํ•œ๊ณผ ์ž์•„์‹คํ˜„์„ฑ์ •๋„์™€์˜ ๊ด€๊ณ„๋Š” ์œ ์˜ํ•œ ๊ฒƒ์œผ๋กœ ๋‚˜ํƒ€๋‚ฌ๋‹ค. 2. ์ผ๋ฐ˜์  ํŠน์„ฑ๊ณผ ์ง๋ฌด๋งŒ์กฑ๋„์™€์˜ ๊ด€๊ณ„์—์„œ๋Š” ์ตœ์ข…์ถœ์‹ ํ•™๊ต, ์ข…๊ต์œ ๋ฌด, ๊ทผ๋ฌด๋…„ํ•œ๊ณผ ์ง๋ฌด๋งŒ์กฑ๋„์™€์˜ ๊ด€๊ณ„๊ฐ€ ์œ ์˜ํ•œ ๊ฒƒ์œผ๋กœ ๋‚˜ํƒ€๋‚ฌ๋‹ค. 3. ์ž์•„์‹คํ˜„์„ฑ์ •๋„์™€ ์ง๋ฌด๋งŒ์กฑ๋„์™€์˜ ๊ด€๊ณ„์—์„œ๋Š” ๋Œ€์ƒ์ž์˜ ๋‘์ง‘๋‹จ ๊ฐ„์— ์ผ๋ฐ˜์  ํŠน์„ฑ์—์„œ ์œ ์˜ํ•œ ๊ฒƒ์œผ๋กœ ๋‚˜ํƒ€๋‚œ ๋Œ€์ƒ์ž์˜ ์—ฐ๋ น, ๊ทผ๋ฌด๋…„ํ•œ ๊ฒฐํ˜ผ์—ฌ๋ถ€, ์ตœ์ข…์ถœ์‹ ํ•™๊ต๋ฅผ ํ†ต์ œํ•œ ๊ฒฐ๊ณผ, ์ž์•„์‹คํ˜„์„ฑ์ •๋„์™€ ์ง๋ฌด๋งŒ์กฑ๋„๋Š” ์œ ์˜ํ•œ ์ƒ๊ด€๊ด€๊ณ„๋ฅผ ๋ณผ ์ˆ˜ ์žˆ์—ˆ๋‹ค(r=.1242, p<05). A Study on Relationship between the Value of Self-actualization and Job Satisfaction of Registerd Nurses Chung, Hyun Sook Graduate School of Health Science and Management Yonsei University (Directed by Professor Han, Jung-Suk) The purpose of this study was to analyze the relationship between the value of self-actualization and job-satisfaction of nurses in comprehensive nursing performance. The subjects for this study were from 264 nurses who were working at four general hospitals and five public health centers located in Seoul. The instruments used for this study were Kim and Lee's self-actualization test, Brayfield and Rothe's job satisfaction scale. The period for the data was collected from 1st April to 15th April, 1984. The collected data was analyzed by means of percentage, ANOVA, t-test, x**2-test, peason correlation coefficient and partial correlation coefficient. The results were as follows : 1. There was a significant association noted between the value self-actualization and job satisfaction on the effect of age, their length of career, marital status and educational background were controlled (r=.1242, p<.05). 2. There was a significant difference between the value of self-actualization and general characteristics such as age marital status, religion and their length of career. 3. There was a significant difference between job satisfaction and general characteristics such as educational background, religion and their length of career. [์˜๋ฌธ] The purpose of this study was to analyze the relationship between the value of self-actualization and job-satisfaction of nurses in comprehensive nursing performance. The subjects for this study were from 264 nurses who were working at four general hospitals and five public health centers located in Seoul. The instruments used for this study were Kim and Lee's self-actualization test, Brayfield and Rothe's job satisfaction scale. The period for the data was collected from 1st April to 15th April, 1984. The collected data was analyzed by means of percentage, ANOVA, t-test, x**2-test, peason correlation coefficient and partial correlation coefficient. The results were as follows : 1. There was a significant association noted between the value self-actualization and job satisfaction on the effect of age, their length of career, marital status and educational background were controlled (r=.1242, p<.05). 2. There was a significant difference between the value of self-actualization and general characteristics such as age marital status, religion and their length of career. 3. There was a significant difference between job satisfaction and general characteristics such as educational background, religion and their length of career.restrictio

    ์˜๋ฅ˜์ œํ’ˆ๋ณ„ ์ ํฌํ˜ผํ•ฉ์• ๊ณ ํ–‰๋™๊ณผ ์ด๋ฅผ ํ†ตํ•œ ํŒจ์…˜์ ํฌ์œ ํ˜•๊ฐ„ ๊ฒฝ์Ÿ๊ตฌ์กฐ๋ถ„์„

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    ํ•™์œ„๋…ผ๋ฌธ(์„์‚ฌ)--์„œ์šธ๋Œ€ํ•™๊ต ๋Œ€ํ•™์› :์˜๋ฅ˜ํ•™๊ณผ,1999.Maste

    HUVECs์™€ monocytes์—์„œ TF ๋ฐœํ˜„์— cilostazol์ด ๋ฏธ์น˜๋Š” ์˜ํ–ฅ

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    Dept. of Medicine/์„์‚ฌ[ํ•œ๊ธ€] ์กฐ์ง์ธ์ž (Tissue factor: TF )๋Š” ํ˜ˆ๊ด€๋ฒฝ ๋‚ด์— ์กด์žฌํ•˜์—ฌ ํ˜ˆ์•ก์‘๊ณ  ๊ณผ์ •์„ ๊ฐœ์‹œํ•˜๋Š”๋ฐ ๋งค์šฐ ์ค‘์š”ํ•˜๋ฉฐ, ํ˜ˆ๊ด€์„ฑํ˜•ํ›„์˜ ํ˜ˆ์ „์ƒ์„ฑ๊ณผ ์žฌํ˜‘์ฐฉ์—๋„ ๊ด€์—ฌํ•˜๋Š” ๊ฒƒ์œผ๋กœ ์•Œ๋ ค์ ธ ์žˆ๋‹ค. TF๋Š” ํ˜ˆ๊ด€๋‚ดํ”ผ์„ธํฌ๋ฟ๋งŒ ์•„๋‹ˆ๋ผ ๋‹จํ•ต๊ตฌ, ๋Œ€์‹์„ธํฌ์™€ ๊ฐ™์€ ๊ณ ์œ ๋ฉด์—ญ์„ธํฌ์— ๋ฐœํ˜„ํ•˜๋ฉฐ, ํŠนํžˆ ์—ผ์ฆ๊ณผ ๊ฐ™์€ ๋ณ‘์ ์ธ ํ™˜๊ฒฝ์—์„œ๋Š” TF๋ฐœํ˜„์ด ํ˜„์ €ํžˆ ์ฆ๊ฐ€ํ•˜๋ฉฐ ์ด๋กœ ์ธํ•ด ๊ธ‰์„ฑ ๊ด€์ƒ๋™๋งฅ์งˆํ™˜์ด๋‚˜ ํ˜ˆ๊ด€์„ฑํ˜•ํ›„์˜ ์žฌํ˜‘์ฐฉ ๋“ฑ์˜ ์ง์ ‘ ์›์ธ์ด ๋œ๋‹ค. ๊ทธ๋Ÿฌ๋ฏ€๋กœ ์ตœ๊ทผ์— TF๋ฅผ ํ‘œ์ ์œผ๋กœ ์ด ์ธ์ž์˜ ๋ฐœํ˜„์„ ์ œ์–ดํ•˜๋Š” ์•ฝ๋ฌผ์š”๋ฒ• ๋“ฑ์ด ๋งŽ์€ ๊ด€์‹ฌ์„ ๋Œ๋ฉฐ ๊ฐœ๋ฐœ๋˜๊ณ  ์žˆ๋‹ค. ๋ณธ ์—ฐ๊ตฌ์—์„œ ์‚ฌ์šฉํ•œ cilostazol์€ AMP-PDE ์–ต์ œ์ธ์ž๋กœ์„œ ํ•ญ ํ˜ˆ์ „, ํ˜ˆ๊ด€ํ™•์žฅ, ์‹ฌ๊ทผ์ˆ˜์ถ•๋“ฑ ์ˆœํ™˜๊ธฐ๊ณ„ ์ž‘์šฉ ๋“ฑ์ด ์ž˜ ์•Œ๋ ค์ ธ ์žˆ๋‹ค. ์ž„์ƒ์ ์œผ๋กœ ๋„๋ฆฌ ์‚ฌ์šฉ๋˜๋‚˜ ์ˆœํ™˜๊ธฐ๊ณ„ ๋ฏธ์น˜๋Š” ์˜ํ–ฅ ์ค‘ ํŠนํžˆ TF ๋ฐœํ˜„๊ณผ ๊ด€๋ จ ์ง€์–ด cilostazol์˜ ์‹œํ—˜๊ด€ ๋ฐ ์ƒ์ฒด ์‹คํ—˜ ๋ฐ ๊ทธ ์ค‘์š”์„ฑ์— ๋Œ€ํ•ด์„œ ๋…ผ์˜๋œ ๋ณด๊ณ ๋Š” ์ „๋ฌดํ•˜๋‹ค. ์ด์— ์ด์˜ ์—ฐ๊ด€์„ฑ์„ ์ž…์ฆํ•˜๋Š” ๊ธฐ์ดˆ์—ฐ๊ตฌ๋กœ HUVECS ์™€ monocyte์—์„œ์˜ TF ๋ฐœํ˜„์— ๊ด€ํ•œ cilostazol์˜ ํšจ๊ณผ๋ฅผ ๊ฒ€์ฆํ•˜๊ณ ์ž ๋ณธ ์—ฐ๊ตฌ๋ฅผ ์‹œ๋„ํ•˜์˜€๋‹ค. ์ธ์ฒด ์ œ๋Œ€ํ˜ˆ๊ด€๋‚ดํ”ผ์„ธํฌ(HUVECS: human umbilical endothelial cell) ๊ณผ ๋‹จํ•ต๊ตฌ (Monocyte)์„ ๊ฑด๊ฐ•ํ•œ ์„ฑ์ธ์œผ๋กœ๋ถ€ํ„ฐ ๋ถ„๋ฆฌ, ์ดˆ๋ฒŒ๋ฐฐ์–‘(primary culture)์„ ํ•œ ํ›„์— ์ฒซ๋ฒˆ์งธ๋กœ TF์˜ mRNA ๋ฐœํ˜„์„ ๋ณด๊ธฐ์œ„ํ•ด์„œ PCR์„ ์‹œํ–‰ํ•˜์˜€๊ณ  ๋‘˜์งธ๋กœ TF ๋‹จ๋ฐฑ ๋ฐœํ˜„์„ ๊ทœ๋ช…ํ•˜๊ธฐ ์œ„ํ•ด์„œ SDS-PAGE ๋ฒ•์„ ์‹œํ–‰ํ•œ ํ›„์— TF, tubulin Ab๋ฅผ ์ด์šฉํ•˜์—ฌ ์›จ์Šคํ„ด ๋ธ”๋กฏ๋ฒ•(Western blotting) ์„ ์—ฐ์†์ ์œผ๋กœ ์‹œํ–‰ํ•˜์˜€๋‹ค. ์ฒซ๋ฒˆ์งธ ๊ฒฐ๊ณผ๋กœ ์šฐ์„  HUVECS์„ ๋Œ€์ƒ์œผ๋กœ TNF-&#61537;๋กœ ์ž๊ทน์‹œํ‚จ ์„ธํฌ์— ๊ฐ๊ฐ cilostazol์˜ ๋†๋„๋ฅผ ๋‹ฌ๋ฆฌํ•˜์—ฌ 4์‹œ๊ฐ„ ๋™์•ˆ ๋ฐฐ์–‘, TF PCR์„ ์‹œํ–‰ํ•œ ๊ฒฐ๊ณผ cilostazol์˜ ๋†๋„์— ๋น„๋ก€ํ•˜์—ฌ TF๋ฐœํ˜„์ด ์–ต์ œ๋จ์„ ๊ด€์ฐฐํ•  ์ˆ˜ ์žˆ์—ˆ๋‹ค. LPS๋กœ ์ž๊ทน์‹œํ‚จ ๋‹จํ•ต๊ตฌ์—์„œ๋„ ์œ„์˜ ์ œ๋Œ€ํ˜ˆ ์„ธํฌ์™€ ๊ฐ™์€ ์–‘์ƒ์„ ๋ณด์˜€๋‹ค. ๊ณตํžˆ ๋‘ ์„ธํฌ์—์„œ 24์‹œ๊ฐ„ ์„ธํฌ ๋ฐฐ์–‘ ํ›„ ์‹ค์‹œํ•œ Western blot analysis๋ฅผ ํ†ตํ•˜์—ฌ TF protein์„ ํ™•์ธํ•œ ๊ฒฐ๊ณผ cilostazol์˜ ๋†๋„์— ๋น„๋ก€ํ•˜์—ฌ TF protein์ด ์–ต์ œ๋จ์„ ์•Œ ์ˆ˜ ์žˆ์—ˆ๋‹ค. ๊ฒฐ๋ก ์ ์œผ๋กœ cilostazol์€ HUVECS์™€ monocyte์—์„œ TF-mRNA์™€ TF protein์˜ ๋ฐœํ˜„์„ ์–ต์ œํ•˜๋Š” ๊ธฐ์ „์„ ๊ฐ–๋Š” ๊ฒƒ์œผ๋กœ ์ƒ๊ฐ๋œ๋‹ค. [์˜๋ฌธ]Tissue factor (TF) is a potent initiator of the coagulation cascade situated within the vessel wall as well as an important key sentinel in the pathogenesis of thrombosis and restenosis after balloon angioplasty. While TF is expressed not only on endothelial cell but also on innate immunocytes such as monocytes and macrophages, pathogenic environment culminates TF expression and release, leading to acute coronary syndrome or restenosis after balloon angioplasty. Recent basic studies focus on the therapeutic inhibition of tissue factor. Cilostazol, even though it is classically known as AMP-PDE inhibitor, is clinically effective because of its possible antiplatelet, vasodilating, antimitogenic, and cardiotonic action. However, there were no known documents regarding usage or significance of cilostazol in direct relation with TF expression. To clarify this issue, we primarily examined the effect of the cilostazol on TF expression in TNF-ฮฑ stimulated HUVECs and LPS-stimulated monocytes. HUVECs and CD14+ monocytes isolated from whole blood of healthy volunteers were incubated for 30minutes with or without cilostazol (1, 10, 100 ฮผM). Thereafter, HUVECs were treated with TNF-ฮฑ (10 ng/ml) and CD14 + monocytes with LPS (1 ฮผg/ml), and incubated&#61537; for 4 hours for RT-PCR or for 24 hours for Western blotting. As a result, expression of TF-mRNA and TF in TNF-ฮฑ stimulated HUVECs was down regulated by cilostazol pretreatment in dose dependant manner. This down regulation in TF-mRNA and TF protein was similar in CD14+ monocytes. In conclusion, these data indicate that cilostazol act as a TF inhibitor on HUVECs and monocytes by down regulating the TF-mRNA and TF protein expression.ope

    Hairdressing service consumers` dualistic loyalty to srevice person and store

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    ํ•™์œ„๋…ผ๋ฌธ(๋ฐ•์‚ฌ)--์„œ์šธ๋Œ€ํ•™๊ต ๋Œ€ํ•™์› :์˜๋ฅ˜ํ•™๊ณผ,2006.Docto
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