Preparation of PLGA microparticles by a double emulsion solvent technique for sustained release of (-)-epigallocatechin gallate (EGCG) and their growth inhibitory effect on rat aortic smooth muscle cells

In this study, we fabricated porous PLGA microparticles loaded with EGCG by a modified water-in-oil-in-water (W/ O/W) double emulsion solvent evaporation method. The physicochemical properties of the EGCG-loaded PLGA microparticles were studied using scanning electron microscopy (SEM), Fourier transformed-infrared (FT-IR) spectroscopy and in vitro release measurements. A remarkable burst effect was observed on first stage however the released amount was reduced largely with time. This result showed that the release of EGCG increased by its loading amount. Also, EGCG - loaded PLGA particles inhibited the growth of rat aortic smooth muslce cells (RASMC). This study demonstrates that PLGA microparticle system is potential as an efficient EGCG carrier in medical therapy.ope

Growth of Smooth Muscle Cell and Endothelial Cell on PLGA Film containing EGCG

Poly (D,L-latic-co-glycolic acid) (PLGA) has been used as the artificial scaffold for blood vessel formation. In order to hinder smooth muscle cell (SMC) angiogenesis, new scaffold design method of loading Epigallocatechin 3-O-gallate (EGCG) on PLGA film was introduced. PLGA and EGCG were dissolved in acetone and film-shape scaffold was manufactured. Antiangiogenetic effect of EGCG released on scaffold was analyzed for SMC and human umbilical vein endothelial cell (HUVEC) and method for selective inhibition from the difference of growth of SMC and HUVEC was suggested.ope

Toxicity Test of Wound Covering Material on Animals

Adhesions are abnormal attachments between tissues, caused by an inflammatory stimulus or trauma. It was generally used physical barriers and various agents to prevent from adhesions formation. In this study, we made an experiment on animals with wound covering material of substance to prevent tissue adhesion. It was performed in sub-acute toxicity, and tested local effects after implantation.ope

Effects Of (1→3), (1→6)-β-D-Glucan Behavior in Human Dermal Fibroblast Cells under Serum Starvation

Glucans have been reported to stimulate immunity and to promote wound healing. Adult human dermal fibroblast (aHDF) cultured in serum free (serum-starvation). Proliferation of aHDF was measured at various concentrations of β-glucan by MTT assay, and migration was observed for 36h on microscope. The result of fibroblast bioassay, β-glucan had positive influence. In this study, the direct effects of β-glucan on proliferation and migration of human dermal fibroblasts were examined in vitro. That means β-D-glucan has the effect to enhance proliferation and aHDF migration speed, and has the potential as a wound healing agent.ope