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    ๋Œ€ํ•ญ์š”๊ฑด์„ ๊ฐ–์ถ”๊ธฐ ์ „ ์ง€๋ช…์ฑ„๊ถŒ์–‘์ˆ˜์ธ์˜ ๋ฒ•์  ์ง€์œ„

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    ํ•™์œ„๋…ผ๋ฌธ (์„์‚ฌ)-- ์„œ์šธ๋Œ€ํ•™๊ต ๋Œ€ํ•™์› : ๋ฒ•ํ•™๊ณผ, 2013. 2. ์œค์ง„์ˆ˜.์ง€๋ช…์ฑ„๊ถŒ์˜ ์–‘๋„๋Š” ์–‘๋„์ธ๊ณผ ์–‘์ˆ˜์ธ ์‚ฌ์ด์˜ ่ซพๆˆๅฅ‘็ด„์— ์˜ํ•˜๋ฏ€๋กœ ์ฑ„๊ถŒ์–‘๋„์— ๊ด€์—ฌํ•˜์ง€ ์•Š์€ ์ฑ„๋ฌด์ž์™€ ์ œ3์ž๋Š” ์ฑ„๊ถŒ์–‘๋„์˜ ์‚ฌ์‹ค์„ ์•Œ์ง€ ๋ชปํ•˜๊ธฐ ๋•Œ๋ฌธ์— ์˜ˆ์ธกํ•˜์ง€ ์•Š์€ ์†ํ•ด๋ฅผ ์ž…์„ ์—ผ๋ ค๊ฐ€ ์žˆ๋‹ค. ์ด๋Ÿฌํ•œ ์ฑ„๋ฌด์ž ๋“ฑ์„ ๋ณดํ˜ธํ•˜๊ธฐ ์œ„ํ•˜์—ฌ ์šฐ๋ฆฌ ๋ฏผ๋ฒ• ์ œ450์กฐ ์ œ1ํ•ญ์€ ๆŒ‡ๅๅ‚ตๆฌŠ์˜ ่ฎ“ๆธก๋Š” ่ฎ“ๅ—ไบบ์ด ๅ‚ตๅ‹™่€…์—๊ฒŒ ้€š็Ÿฅํ•˜๊ฑฐ๋‚˜ ๅ‚ตๅ‹™่€…๊ฐ€ ๆ‰ฟ่ซพํ•˜์ง€ ์•„๋‹ˆํ•˜๋ฉด ๅ‚ตๅ‹™่€… ๅ…ถไป– ็ฌฌไธ‰่€…์—๊ฒŒ ๅฐๆŠ—ํ•˜์ง€ ๋ชป ํ•œ๋‹ค๋ผ๊ณ  ๊ทœ์ •ํ•จ์œผ๋กœ์จ ๋Œ€ํ•ญ์š”๊ฑด์ฃผ์˜๋ฅผ ๋”ฐ๋ฅด๊ณ  ์žˆ๋‹ค. ๋Œ€ํ•ญํ•˜์ง€ ๋ชป ํ•œ๋‹ค๋ผ๋Š” ์กฐ๋ฌธ ๊ทœ์ •์˜ ์˜๋ฏธ์— ๋Œ€ํ•ด ๊ธฐ์กด์˜ ํ•™์„ค์€ ์ฑ„๋ฌด์ž์— ๋Œ€ํ•˜์—ฌ๋Š” ์ฑ„๊ถŒ์–‘๋„์˜ ํšจ๋ ฅ์ด ์—†๋‹ค๊ณ  ๋ณด๋Š” ์ƒ๋Œ€์  ๋ฌดํšจ์„ค์ด ๋‹ค์ˆ˜์„ค์ด์—ˆ๊ณ , ํŒ๋ก€๋„ ๋Œ€์ฒด์ ์œผ๋กœ ์ด ์„ค์„ ๋”ฐ๋ž๋‹ค. ๊ทธ๋Ÿฌ๋‚˜ ์ตœ๊ทผ ๋Œ€๋ฒ•์› ํŒ๋ก€๋Š” ๋Œ€ํ•ญ์š”๊ฑด์„ ๊ฐ–์ถ”์ง€ ๋ชปํ•œ ์ฑ„๊ถŒ์˜ ์–‘์ˆ˜์ธ์ด๋ผ๊ณ  ํ•˜๋”๋ผ๋„ ์ฑ„๋ฌด์ž๋ฅผ ์ƒ๋Œ€๋กœ ์žฌํŒ์ƒ์˜ ์ฒญ๊ตฌ๋ฅผ ํ•˜์˜€๋‹ค๋ฉด ์ด๋Š” ์†Œ๋ฉธ์‹œํšจ ์ค‘๋‹จ์‚ฌ์œ ์ธ ์žฌํŒ์ƒ์˜ ์ฒญ๊ตฌ์— ํ•ด๋‹นํ•œ๋‹ค๊ณ  ๋ณด์•„์•ผ ํ•œ๋‹ค๊ณ  ํŒ์‹œํ•˜๋Š” ๋“ฑ ๋” ์ด์ƒ ๋Œ€๋ฒ•์› ํŒ๋ก€๊ฐ€ ์ƒ๋Œ€์  ๋ฌดํšจ์„ค์˜ ์ž…์žฅ์„ ์ทจํ•˜๊ณ  ์žˆ๋‹ค๊ณ  ์ž”์ •ํ•˜๊ธฐ ์–ด๋ ต๊ฒŒ ๋˜์—ˆ๊ณ , ๋Œ€ํ•ญํ•˜์ง€ ๋ชป ํ•œ๋‹ค๋Š” ์˜๋ฏธ๊ฐ€ ์–ด๋–ป๊ฒŒ ํ•ด์„๋˜๋Š”์ง€์— ๋”ฐ๋ผ ์ฑ„๋ฌด์ž ๋ฐ ์ฑ„๊ถŒ์–‘๋„์ธ, ์–‘์ˆ˜์ธ์˜ ๊ถŒ๋ฆฌ๊ตฌ์ œ์˜ ๋ฒ”์œ„๊ฐ€ ๋‹ฌ๋ผ์งˆ ์ˆ˜ ์žˆ์œผ๋ฏ€๋กœ ์ด ์˜๋ฏธ๋ฅผ ์ƒˆ๋กญ๊ฒŒ ์žฌ์กฐ๋ช…ํ•ด๋ณด์•„์•ผ ํ•  ํ•„์š”์„ฑ์ด ๋Œ€๋‘๋˜์—ˆ๋‹ค. ์šฐ๋ฆฌ ๋ฏผ๋ฒ•์€ ๋ฌผ๊ถŒ๋ณ€๋™์— ๊ด€ํ•˜์—ฌ์„œ๋งŒ ์„ฑ๋ฆฝ์š”๊ฑด์ฃผ์˜๋ฅผ ์ทจํ•˜๊ณ  ์žˆ๊ณ  ์ฑ„๊ถŒ์–‘๋„์— ๊ด€ํ•˜์—ฌ๋Š” ๋‹จ์ง€ ์–‘๋„ํ†ต์ง€ ๋“ฑ์„ ๋Œ€ํ•ญ์š”๊ฑด์œผ๋กœ๋งŒ ๊ทœ์ •ํ•˜๊ณ  ์žˆ์„ ๋ฟ์ด๋‹ค. ์ฑ„๊ถŒ์–‘๋„์—์„œ๋Š” ์ฑ„๊ถŒ์–‘๋„์ธ๊ณผ ์–‘์ˆ˜์ธ ๋‹น์‚ฌ์ž์˜ ํ•ฉ์˜๋งŒ์œผ๋กœ ๊ทธ ์–‘๋„์˜ ํšจ๋ ฅ์„ ์ธ์ •ํ•˜๋˜, ์ฑ„๊ถŒ์–‘๋„ ๊ณผ์ •์—์„œ ๊ด€์—ฌํ•  ์ˆ˜ ์—†์—ˆ๋˜ ์ฑ„๋ฌด์ž ๋ฐ ์ œ3์ž๋ฅผ ๋ณดํ˜ธํ•˜๊ธฐ ์œ„ํ•˜์—ฌ ๋Œ€ํ•ญ์š”๊ฑด์„ ๊ทœ์ •ํ•˜์—ฌ ๋†“์•˜์„ ๋ฟ์ด๋ฏ€๋กœ ๋Œ€ํ•ญ์š”๊ฑด์€ ๋ฌผ๊ถŒ์˜ ์žฌ์‚ฐ๊ถŒ๋ณ€๋™์— ์žˆ์–ด์„œ ๊ณต์‹œ ๊ธฐ๋Šฅ์„ ๊ฐ€์ง€๊ณ  ์žˆ๋Š” ๊ฒƒ์ด ์•„๋‹ˆ๋‹ค. ๋•Œ๋ฌธ์— ์ฑ„๋ฌด์ž์— ๋Œ€ํ•œ ๊ด€๊ณ„์—์„œ๋„ ๋Œ€ํ•ญ์š”๊ฑด์„ ๊ฐ–์ถ”์ง€ ๋ชปํ•œ ์ฑ„๊ถŒ์–‘์ˆ˜์ธ์€ ์ฑ„๊ถŒ์–‘๋„์˜ ํšจ๋ ฅ์— ์˜ํ•˜์—ฌ ์ฑ„๊ถŒ์ž๋กœ์„œ์˜ ์ง€์œ„๋ฅผ ๊ฐ€์ง€๊ฒŒ ๋˜๋ฉฐ, ๋‹จ์ง€ ์ฑ„๋ฌด์ž ์ธก์—์„œ ๋Œ€ํ•ญ์š”๊ฑด์˜ ๋ถˆ๋น„๋ฅผ ์ด์œ ๋กœ ํ•˜์—ฌ ์ฑ„๊ถŒ์–‘์ˆ˜์ธ์˜ ์ฒญ๊ตฌ๋ฅผ ๊ฑฐ์ ˆํ•  ์ˆ˜ ์žˆ๋Š” ๊ถŒ๋ฆฌ๊ฐ€ ์žˆ๋Š” ๊ฒƒ์ด๋ผ๊ณ  ๋ณด๋Š” ๊ฒƒ์ด ํƒ€๋‹นํ•˜๊ณ  ์ด๊ฒƒ์ด ๋ฐ”๋กœ ๋ฏผ๋ฒ• ์ œ450์กฐ ์ œ1ํ•ญ์—์„œ ๊ทœ์ •ํ•˜๊ณ  ์žˆ๋Š” ๋Œ€ํ•ญํ•˜์ง€ ๋ชป ํ•œ๋‹ค๋ผ๋Š” ์˜๋ฏธ๋ผ๊ณ  ํ•  ๊ฒƒ์ด๋‹ค. ๋”ฐ๋ผ์„œ ๋Œ€ํ•ญ์š”๊ฑด์„ ๊ฐ–์ถ”์ง€ ๋ชปํ•œ ์ฑ„๊ถŒ์–‘์ˆ˜์ธ์ด๋ผ๊ณ  ํ•˜๋”๋ผ๋„ ์ฑ„๋ฌด์ž๊ฐ€ ๋Œ€ํ•ญ์š”๊ฑด์˜ ๋ถˆ๋น„๋ฅผ ์ด์œ ๋กœ ํ•˜์—ฌ ์ฑ„๊ถŒ์–‘์ˆ˜์ธ์˜ ์ฒญ๊ตฌ๋ฅผ ๊ฑฐ์ ˆํ•˜๋Š” ๊ถŒ๋ฆฌ ๋“ฑ์„ ํ–‰์‚ฌํ•˜์ง€ ์•Š๋Š” ํ•œ ์ฑ„๊ถŒ์–‘์ˆ˜์ธ์€ ์ฑ„๊ถŒ์–‘๋„์˜ ์‹œ์ ๋ถ€ํ„ฐ ์ฑ„๋ฌด์ž์—๊ฒŒ ์ฑ„๊ถŒ์ž๋กœ์„œ์˜ ์ง€์œ„๋ฅผ ๊ฐ€์ง€๊ฒŒ ๋˜๊ณ , ๊ทธ์— ๋”ฐ๋ผ ์‹œํšจ์ค‘๋‹จ ํ–‰์œ„, ๋‹ด๋ณด๊ถŒ์˜ ์‹คํ–‰ ๋“ฑ์„ ํ•  ์ˆ˜ ์žˆ๋‹ค๊ณ  ๋ณด์•„์•ผ ํ•˜๋Š” ๊ฒƒ์ด๋ฉฐ, ํŒ๋ก€ ์—ญ์‹œ ์ง€๊ธˆ์˜ ํ˜ผ์žฌ๋œ ์ž…์žฅ์—์„œ ์ฑ„๊ถŒ์–‘์ˆ˜์ธ์˜ ์ ๊ทน์ ์ธ ์ฑ„๊ถŒ์ž์˜ ์ง€์œ„๋ฅผ ์ธ์ •ํ•˜๋Š” ๋ฐฉํ–ฅ์œผ๋กœ ๋‚˜์•„๊ฐ€์•ผ ํ•˜๋Š” ๊ฒƒ์ด ํƒ€๋‹นํ•  ๊ฒƒ์ด๋‹ค.์ œ1์žฅ ์„œ๋ก  โ… . ๋…ผ์˜์˜ ํ•„์š”์„ฑ 1. ์ฑ„๊ถŒ์–‘๋„์— ๊ด€ํ•œ ์šฐ๋ฆฌ ๋ฏผ๋ฒ•์˜ ๊ทœ์ • 2. ์ฑ„๋ฌด์ž ๋“ฑ์— ๋Œ€ํ•œ ๋ณดํ˜ธ๋ฐฉ์•ˆ 3. ํŒ๋ก€์˜ ๋ณ€ํ™”์™€ ๋…ผ์˜์˜ ํ•„์š”์„ฑ ๋Œ€๋‘ โ…ก. ๋…ผ์˜์˜ ์ „๊ฐœ์™€ ๊ตฌ์„ฑ ์ œ2์žฅ ์™ธ๊ตญ์˜ ์ž…๋ฒ•๋ก€ โ… . ํ”„๋ž‘์Šค๋ฒ•์—์„œ์˜ ๋…ผ์˜ 1. ๊ณ„์•ฝ์ƒ๋Œ€ํšจ์˜ ์›์น™ 2. ํญ๋„“์€ ์˜ˆ์™ธ์˜ ์ธ์ • ๊ฐ€. ์ผ์ •ํ•œ ์š”๊ฑด ํ•˜์˜ ์ดํ–‰์ฒญ๊ตฌ ๋‚˜. ๋ณด์กดํ–‰์œ„ โ…ก. ์ผ๋ณธ๋ฒ•์—์„œ์˜ ๋…ผ์˜ 1. ์ผ๋ณธ์˜ ํ•™์„ค ๊ฐ€. ์ƒ๋Œ€์  ๋ฌดํšจ์„ค ๋‚˜. ๋ถ€์ธ๊ถŒ์„ค 2. ์ผ๋ณธ์˜ ํŒ๋ก€ ๊ฐ€. ๋Œ€์‹ฌ์› ๋‚˜. ์ตœ๊ณ ์žฌํŒ์†Œ[ๆœ€้ซ˜่ฃ็ฌฌ3ๅฐๆณ•ๅฎš ๆ˜ญๅ’Œ56ๅนด(1981๋…„) 10ๆœˆ13ๆ—ฅ, ๆ˜ญ56(ใ‚ช)483ๅท ๅˆคๆฑบ] 3. ์†Œ๊ฒฐ ์ œ3์žฅ ์šฐ๋ฆฌ๋‚˜๋ผ์—์„œ์˜ ๋…ผ์˜ โ… . ํ•™์„ค์˜ ๋…ผ์˜ 1. ํšจ๋ ฅ๋ถˆ๋ฐœ์ƒ์„ค 2. ์ƒ๋Œ€์  ๋ฌดํšจ์„ค 3. ํšจ๋ ฅ๋ฐœ์ƒ์„ค โ…ก. ํŒ๋ก€์˜ ์ž…์žฅ 1. ๋Œ€๋ฒ•์› 1992. 8. 18. ์„ ๊ณ  90๋‹ค9452 ํŒ๊ฒฐ 2. ๋Œ€๋ฒ•์› 2000. 12. 12. ์„ ๊ณ  2000๋‹ค1006 ํŒ๊ฒฐ ๊ฐ€. ํšจ๋ ฅ๋ถˆ๋ฐœ์ƒ์„ค๊ณผ ์ƒ๋Œ€์  ๋ฌดํšจ์„ค, ํšจ๋ ฅ๋ฐœ์ƒ์„ค ๋ชจ๋‘๋กœ ํ•ด์„ ๊ฐ€๋Šฅํ•˜๋‹ค๊ณ  ๋ณด๋Š” ๊ฒฌํ•ด ๋‚˜. ํšจ๋ ฅ๋ฐœ์ƒ์„ค์„ ์ทจํ•˜์˜€๋‹ค๊ณ  ๋ณด๋Š” ๊ฒฌํ•ด 3. ๋Œ€๋ฒ•์› 2005. 11. 10. ์„ ๊ณ  2005๋‹ค41818 ํŒ๊ฒฐ 4. ๋Œ€๋ฒ•์› 2009. 2. 12. ์„ ๊ณ  2008๋‘20109 ํŒ๊ฒฐ 5. ๊ทธ ๋ฐ–์˜ ์ €๋‹น๊ถŒ๋ถ€ ์ฑ„๊ถŒ์–‘๋„์— ๊ด€ํ•œ ํŒ๋ก€ โ…ข. ๊ฒ€ํ†  1. ํšจ๋ ฅ๋ถˆ๋ฐœ์ƒ์„ค์— ๋Œ€ํ•œ ๋น„ํŒ 2. ์ƒ๋Œ€์  ๋ฌดํšจ์„ค์— ๋Œ€ํ•œ ๋น„ํŒ 3. ์†Œ๊ฒฐ ์ œ4์žฅ ์ €๋‹น๊ถŒ๋ถ€ ์ฑ„๊ถŒ์–‘๋„์™€ ์ฑ„๊ถŒ์–‘์ˆ˜์ธ์˜ ๋ฒ•์  ์ง€์œ„ โ… . ์„œ 1. ์ €๋‹น๊ถŒ์˜ ๋ถ€์ข…์„ฑ 2. ์ €๋‹น๊ถŒ ์‹คํ–‰๊ฐ€๋Šฅ์„ฑ ๊ฐ€. ๋‹ด๋ณด๊ถŒ ์‹คํ–‰์„ ์œ„ํ•œ ๊ฒฝ๋งค์‹ ์ฒญ์„ ๋น„์†ก์‚ฌ๊ฑด์ด๋ผ๊ณ  ๋ณด๋Š” ๊ฒฌํ•ด ๋‚˜. ์ ˆ์ฐจ๋ฒ•์ ์ธ ์„ฑ๊ฒฉ๊ณผ ์‹ค์ฒด๋ฒ•์ ์ธ ์„ฑ๊ฒฉ ๋ชจ๋‘ ์ง€๋‹Œ๋‹ค๊ณ  ๋ณด๋Š” ๊ฒฌํ•ด ๋‹ค. ์†Œ๊ฒฐ โ…ก. ํŒ๋ก€์˜ ํƒœ๋„ 1. ๋Œ€๋ฒ•์› 1964. 4. 28.์ž 63๋งˆ216 ๊ฒฐ์ • 2. ๋Œ€๋ฒ•์› 2005. 6. 23. ์„ ๊ณ  2004๋‹ค29279 ํŒ๊ฒฐ ๊ฐ€. ์‚ฌ์‹ค๊ด€๊ณ„ ๋‚˜. ์›๊ณ ์˜ ์ด์ค‘๊ฒฝ๋งค ์‹ ์ฒญ ์ฃผ์žฅ ๋‹ค. ์›์‹ฌ์˜ ํŒ๋‹จ ๋ผ. ๋Œ€๋ฒ•์›์˜ ํŒ๋‹จ 3. ๋Œ€๋ฒ•์› 2005. 6. 23. ์„ ๊ณ  2004๋‹ค29279 ํŒ๊ฒฐ์— ๋Œ€ํ•œ ๊ฒ€ํ†  โ…ข. ๊ด€๋ จ ๋ฏผ์‚ฌ์ง‘ํ–‰๋ฒ• ๊ทœ์ •์˜ ๊ฒ€ํ†  1. ๊ด€๋ จ ๊ทœ์ • ๊ฐœ๊ด€ 2. ๋ฏผ์‚ฌ์ง‘ํ–‰๋ฒ• ์ œ264์กฐ 3. ๊ฒฝ๋งค๊ฐœ์‹œ๊ฒฐ์ • ์ดํ›„ ์ดํ•ด๊ด€๊ณ„์ธ์˜ ๋Œ€์‘๋ฐฉ๋ฒ• ๊ฐ€. ๊ฒฝ๋งค๊ฐœ์‹œ๊ฒฐ์ •์— ๋Œ€ํ•œ ์ด์˜ ๋‚˜. ๋งค๊ฐํ—ˆ๊ฐ€์— ๋Œ€ํ•œ ์ด์˜ ๋ฐ ๋งค๊ฐํ—ˆ๊ฐ€๊ฒฐ์ •์— ๋Œ€ํ•œ ํ•ญ๊ณ  โ…ฃ. ๊ฒฐ ์ œ5์žฅ ์ฑ„๊ถŒ์–‘์ˆ˜์ธ์˜ ์žฌํŒ์ƒ ์ฒญ๊ตฌ์™€ ์‹œํšจ์ค‘๋‹จ โ… . ์„œ 1. ์†Œ๋ฉธ์‹œํšจ์ œ๋„ ๊ฐœ๊ด€ ๊ฐ€. ์‹œํšจ์˜ ์˜์˜ ๋‚˜. ์‹œํšจ์ œ๋„์˜ ์กด์žฌ์ด์œ  2. ์†Œ๋ฉธ์‹œํšจ ์ค‘๋‹จ์˜ ์˜์˜ ๋ฐ ๊ทผ๊ฑฐ ๊ฐ€. ์‹œํšจ์ค‘๋‹จ์˜ ์˜์˜ ๋‚˜. ์‹œํšจ์ค‘๋‹จ์˜ ๊ทผ๊ฑฐ 3. ์žฌํŒ์ƒ ์ฒญ๊ตฌ์™€ ์‹œํšจ์ค‘๋‹จ์˜ ๊ทผ๊ฑฐ ๊ฐ€. ์‹œํšจ์ค‘๋‹จ ์‚ฌ์œ ๋กœ์„œ์˜ ์žฌํŒ์ƒ ์ฒญ๊ตฌ ๋‚˜. ์‹œํšจ์ค‘๋‹จ์˜ ๊ทผ๊ฑฐ ๋‹ค. ์‹œํšจ์ค‘๋‹จํšจ๊ณผ์˜ ์†Œ๋ฉธ - ๋ฏผ๋ฒ• ์ œ170์กฐ ์ œ1ํ•ญ์˜ ์ฒญ๊ตฌ๊ธฐ๊ฐ ๋ฌธ์ œ โ…ก. ํŒ๋ก€์˜ ์ž…์žฅ 1. ๋Œ€๋ฒ•์› 2005. 11. 10. ์„ ๊ณ  2005๋‹ค41818 ํŒ๊ฒฐ 2. ๋Œ€๋ฒ•์› 2009. 2. 12. ์„ ๊ณ  2008๋‘20109 ํŒ๊ฒฐ 3. ์œ„ ์–‘ ํŒ๋ก€์˜ ๋ฌธ์ œ์  โ…ข. ํŒ๋ก€์— ๋Œ€ํ•œ ๊ฒ€ํ†  1. ๋Œ€ํ•ญ์š”๊ฑด์„ ๊ฐ–์ถ”๊ธฐ ์ „์˜ ์ฑ„๊ถŒ์–‘์ˆ˜์ธ์˜ ์žฌํŒ์ƒ ์ฒญ๊ตฌ๊ฐ€ ์‹œํšจ์ค‘๋‹จ ์‚ฌ์œ ์— ํ•ด๋‹นํ•˜๋Š”์ง€ ์—ฌ๋ถ€ 2. ์ฑ„๊ถŒ์–‘๋„์ธ์ด ๋Œ€ํ•ญ์š”๊ฑด์„ ๊ฐ–์ถ”๊ธฐ ์ „์— ์žฌํŒ์ƒ ์ฒญ๊ตฌํ•˜๋Š” ๊ฒฝ์šฐ ์‹œํšจ์ค‘๋‹จ ์‚ฌ์œ ์— ํ•ด๋‹นํ•˜๋Š”์ง€ ์—ฌ๋ถ€ ๊ฐ€. ์ฑ„๊ถŒ์–‘๋„์ธ์ด ์–‘๋„ ์ „ ์žฌํŒ์ƒ ์ฒญ๊ตฌ๋ฅผ ํ•œ ๊ฒฝ์šฐ ๋‚˜. ์ฑ„๊ถŒ์–‘๋„์ธ์ด ์–‘๋„ ํ›„ ์žฌํŒ์ƒ ์ฒญ๊ตฌ๋ฅผ ํ•œ ๊ฒฝ์šฐ โ…ฃ. ๊ฒฐ ์ œ6์žฅ ์žฅ๋ž˜์ดํ–‰์˜ ์†Œ์™€ ์ฑ„๊ถŒ์–‘์ˆ˜์ธ์˜ ๋ฒ•์ ์ง€์œ„ โ… . ์„œ 1. ์ฑ„๊ถŒ์–‘๋„ ํ†ต์ง€๊ถŒ์ž์˜ ๋ฌธ์ œ 2. ์ฑ„๊ถŒ์–‘์ˆ˜์ธ์˜ ๊ถŒ๋ฆฌ๋ณดํ˜ธ ํ•„์š”์„ฑ โ…ก. ํŒ๋ก€์˜ ํƒœ๋„ 1. ๋Œ€๋ฒ•์› 1992. 8. 18. ์„ ๊ณ  90๋‹ค9452 ํŒ๊ฒฐ ๊ฐ€. ์‚ฌ์‹ค๊ด€๊ณ„ ๋‚˜. ํŒ์‹œ๋‚ด์šฉ โ…ข. ํŒ๋ก€์˜ ํƒœ๋„์— ๋Œ€ํ•œ ๋น„ํŒ 1. ์„œ 2. ์žฅ๋ž˜์ดํ–‰์˜ ์†Œ ๊ฐ€. ์˜์˜ ๋‚˜. ์ฒญ๊ตฌ์˜ ๋Œ€์ƒ ๋‹ค. ๊ถŒ๋ฆฌ๋ณดํ˜ธ์˜ ์ด์ต 3. ์ตœ๊ทผ ํŒ๋ก€์™€์˜ ๋ชจ์ˆœ์  4. ์กฐ๊ฑด๋ถ€ ๊ถŒ๋ฆฌ ์กฐํ•ญ์˜ ์ค€์šฉ ๋ฌธ์ œ ๊ฐ€. ์กฐ๊ฑด๋ถ€ ๊ถŒ๋ฆฌ์˜ ์˜์˜ ๋‚˜. ์†Œ๊ฒฐ โ…ฃ. ๊ฒฐ ์ œ7์žฅ ๊ฒฐ์–ด 90Maste

    Structural Studies of Csd6 Protein from Helicobacter pylori and Rv2258c Protein from Mycobacterium tuberculosis

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    ํ•™์œ„๋…ผ๋ฌธ (๋ฐ•์‚ฌ)-- ์„œ์šธ๋Œ€ํ•™๊ต ๋Œ€ํ•™์› : ํ™”ํ•™์ƒ๋ฌผํ•™๊ณผ, 2016. 8. ์„œ์„ธ์›.Helicobacter pylori causes gastrointestinal diseases, including gastric cancer. Mycobacterium tuberculosis induces tuberculosis, claiming the lives of millions of people in the world every year. Increasing drug-resistance among bacterial pathogens is a serious global health issue. Therefore, discovery of new antimicrobial agents is needed urgently. Identifying the molecular and biological functions of proteins from pathogenic bacteria would provide the groundwork for the development of new antibacterial drug targets. In this study, I have determined the crystal structures of Csd6 from H. pylori and Rv2258c from M. tuberculosis. The H. pylori Csd6 protein plays a key role in determining the helical cell shape by trimming of peptidoglycan muropeptides. Csd6 is also involved in deglycosylation of the flagellar protein FlaA. The structure of Csd6 reveals that its middle catalytic domain resembles those of L,D-transpeptidases but its pocket-shaped active-site shows distinct variations from the known L,D-transpeptidases. Mass analyses confirm that Csd6 functions only as an L,D-carboxypeptidase but not as an L,D-transpeptidase. D-Ala-complexed structure of Csd6 reveals binding modes of both the substrate and product to the catalytic domain. On the basis of structure-function analysis, Csd6 and its homologs are proposed to constitute a new family of L,D-carboxypeptidase. The M. tuberculosis Rv2258c protein is predicted to be an S-adenosyl-L-methionine-dependent methyltransferase (MTase). MTases mediate a wide variety of cellular processes, such as cell signaling, metabolite synthesis, and gene regulation in nearly all living organisms. The structure of Rv2258c shows that its monomer consists of two domains linked by a long ฮฑ-helix. The N-terminal domain is essential for dimerization and the C-terminal catalytic domain has the Class I MTase fold. The overall fold of Rv2258c, as well as its interactions with bound sinefungin (or S-adenosyl-L-homocysteine), are similar to small-molecule MTases. Rv2258c has a relatively large hydrophobic cavity for binding the methyl-accepting substrate, suggesting that bulky nonpolar molecules might be targeted for methylation by Rv2258c in M. tuberculosis.Chapter 1. The cell-shape determining Csd6 protein from Helicobacter pylori constitutes a new family of L,D-carboxypeptidase 1 1.1 Introduction 1 1.2 Material and methods 13 1.2.1 Cloning, expression, and purification 13 1.2.2 Crystallization and X-ray data collection 17 1.2.3 Structure determination and refinement 21 1.2.4 In silico docking 22 1.2.5 Peptidase assay by mass analysis 24 1.2.6 Analytical ultracentrifugation 26 1.2.7 Single-molecule fluorescence resonance energy transfer (FRET) 26 1.2.8 Surface plasmon resonance experiment 28 1.3 Results 30 1.3.1. Csd6 functions as an LD-CPase but not as an LD-TPase 30 1.3.2 The Csd6 monomer is organized into three-domain architecture 34 1.3.3 The NTD of Csd6 plays a dominant role in homo-dimerization 37 1.3.4 Active-site of Csd6 LD-CPase domain is tailored for the LD-CPase activity 47 1.3.5 D-Ala-complexed structure reveals binding modes of both the substrate and product 54 1.3.6 The C-terminal NTF2-like domain has a putative binding pocket for pseudaminic acid 61 1.4 Discussion 68 1.5 References 71 Chapter 2. Crystal structure of Rv2258c from Mycobacterium tuberculosis H37Rv, an S-adenosyl-L-methionine-dependent methyltransferase 87 2.1 Introduction 87 2.2 Material and methods 90 2.2.1 Cloning, expression, and purification 90 2.2.2 Crystallization and X-ray data collection 93 2.2.3 Structure determination and refinement 100 2.2.4 Analytical gel filtration 103 2.3 Results 104 2.3.1 Structure determination and comparisons among monomer models of Rv2258c 104 2.3.2 Overall monomer structure of Rv2258c 107 2.3.3 Rv2258c exists as a homodimer 110 2.3.4 Structural similarity search 114 2.3.5 MTase sequence motifs and SFG (or SAH) binding 119 2.3.6 Rv2258c is a unique SAM-dependent MTase in mycobacteria 123 2.3.7 Relatively large substrate-binding cavity of Rv2258c and functional implications 127 2.4 Conclusions 133 2.5 References 134 Abstract (in Korean) 142Docto

    Crystallization and preliminary x-ray crystallographic analyses of the HP0518 protein from helicobacter pylori

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    ํ•™์œ„๋…ผ๋ฌธ (์„์‚ฌ)-- ์„œ์šธ๋Œ€ํ•™๊ต ๋Œ€ํ•™์› : ์ƒ๋ฌผ๋ฌผ๋ฆฌ ๋ฐ ํ™”ํ•™์ƒ๋ฌผํ•™๊ณผ, 2011.8. ์„œ์„ธ์›.Maste

    Emotional labor of care workers and organizational effectiveness

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    ๋ณด๊ฑด๊ด€๋ฆฌํ•™์ „๊ณต/์„์‚ฌ๋ณธ ์—ฐ๊ตฌ๋Š” ์š”์–‘๋ณดํ˜ธ์‚ฌ์˜ ๊ฐ์ •๋…ธ๋™์ˆ˜์ค€์— ์˜ํ–ฅ์„ ๋ฏธ์น˜๋Š” ์š”์ธ์— ๋Œ€ํ•ด ์•Œ์•„๋ณด๊ณ , ๊ฐ์ •๋…ธ๋™๊ณผ ์กฐ์งํšจ๊ณผ์„ฑ๊ณผ์˜ ๊ด€๊ณ„๋ฅผ ํŒŒ์•…ํ•˜๊ณ ์ž ์ˆ˜ํ–‰๋˜์—ˆ๋‹ค. ์ž๋ฃŒ ์ˆ˜์ง‘์€ ์ „๊ตญ 10๊ฐœ ์ง€์—ญ ์†Œ์žฌ 20๊ฐœ ์žฅ๊ธฐ์š”์–‘๊ธฐ๊ด€์— ๊ทผ๋ฌดํ•˜๋Š” ์š”์–‘๋ณดํ˜ธ์‚ฌ 400๋ช…์„ ๋Œ€์ƒ์œผ๋กœ 2013๋…„ 9์›” 25์ผ๋ถ€ํ„ฐ 10์›” 24์ผ๊นŒ์ง€ 30์ผ๊ฐ„ ๋ฐฉ๋ฌธ ๋ฐ ์šฐํŽธ์กฐ์‚ฌ๋ฅผ ํ†ตํ•ด ์ž๊ธฐ๊ธฐ์ž…์‹ ์„ค๋ฌธ์กฐ์‚ฌ๋ฅผ ํ†ตํ•ด ์ด๋ฃจ์–ด์กŒ๋‹ค. ์ด 400๋ถ€ ์ค‘ 365๋ถ€๊ฐ€ ํšŒ์ˆ˜๋˜์–ด ์‘๋‹ต์ด ๋ถˆ์„ฑ์‹คํ•œ 37๋ถ€๋ฅผ ์ œ์™ธํ•˜๊ณ  ์ด 328๋ถ€๋ฅผ ๋ถ„์„ํ•˜์˜€๋‹ค. ์ž๋ฃŒ ๋ถ„์„์€ SPSS 18.0 ํ”„๋กœ๊ทธ๋žจ์„ ์‚ฌ์šฉํ•˜์—ฌ ๊ธฐ์ˆ  ํ†ต๊ณ„๋ถ„์„, T-test, ๋ถ„์‚ฐ๋ถ„์„(ANOVA), ์ƒ๊ด€๊ด€๊ณ„๋ถ„์„ ๋ฐ ๋‹ค์ค‘ํšŒ๊ท€๋ถ„์„์„ ์‚ฌ์šฉํ•˜์˜€๋‹ค. ๋ณธ ์—ฐ๊ตฌ์˜ ๊ฒฐ๊ณผ๋ฅผ ์š”์•ฝํ•˜๋ฉด ๋‹ค์Œ๊ณผ ๊ฐ™๋‹ค. 1) ์š”์–‘๋ณดํ˜ธ์‚ฌ์˜ ๊ฐ์ •๋…ธ๋™ ์ˆ˜์ค€์—๋Š” ๋Œ€์ƒ์ž์š”์ธ๊ณผ ์ƒ์‚ฌ์š”์ธ์ด ์œ ์˜๋ฏธํ•œ ์˜ํ–ฅ์„ ๋ฏธ์น˜๋Š” ๊ฒƒ์œผ๋กœ ๋‚˜ํƒ€๋‚ฌ๋‹ค. 2) ์š”์–‘๋ณดํ˜ธ์‚ฌ์˜ ์—ฐ๋ น์ด ๋‚ฎ์„์ˆ˜๋ก ๊ฐ์ •๋…ธ๋™ ์ˆ˜์ค€์ด ๋†’์•˜๋‹ค 3) ์ง๋ฌด์ŠคํŠธ๋ ˆ์Šค์— ์œ ์˜ํ•œ ์˜ํ–ฅ์„ ๋ฏธ์น˜๋Š” ์š”์ธ์€ ๊ธ์ •์  ์‹ฌ์„ฑ, ๋ถ€์ •์  ์‹ฌ์„ฑ, ์‹œ์„ค์œ ํ˜•, ๊ทผ๋ฌด์‹œ๊ฐ„, ๊ธ‰์—ฌ์˜€์œผ๋ฉฐ, ๊ฐ์ •๋…ธ๋™๊ณผ ๊ฐ์„ฑ์ง€๋Šฅ, ์ž๊ธฐํšจ๋Šฅ๊ฐ์€ ์œ ์˜ํ•œ ์˜ํ–ฅ์„ ๋ฏธ์น˜์ง€ ์•Š์•˜๋‹ค. 4) ์ง๋ฌด์†Œ์ง„์— ์œ ์˜ํ•œ ์˜ํ–ฅ์„ ๋ฏธ์น˜๋Š” ์š”์ธ์€ ๋ถ€์ •์  ์‹ฌ์„ฑ, ์‹œ์„ค์œ ํ˜•, ๊ทผ๋ฌด์‹œ๊ฐ„์ด์—ˆ์œผ๋ฉฐ, ๊ฐ์ •๋…ธ๋™๊ณผ ๊ฐ์„ฑ์ง€๋Šฅ, ์ž๊ธฐํšจ๋Šฅ๊ฐ์€ ์œ ์˜ํ•œ ์˜ํ–ฅ์„ ๋ฏธ์น˜์ง€ ์•Š์•˜๋‹ค. 5) ์ง๋ฌด๋งŒ์กฑ์— ์œ ์˜ํ•œ ์˜ํ–ฅ์„ ๋ฏธ์น˜๋Š” ์š”์ธ์€ ๊ฐ์ •๋…ธ๋™, ๊ธ์ •์  ์‹ฌ์„ฑ, ๋ถ€์ •์  ์‹ฌ์„ฑ, ๊ฐ์„ฑ์ง€๋Šฅ, ์„ฑ๋ณ„, ๊ต์œก์ˆ˜์ค€, ๋Œ๋ด„ ๋Œ€์ƒ์ž์ˆ˜์ด๋ฉฐ, ์ž๊ธฐํšจ๋Šฅ๊ฐ์€ ์œ ์˜ํ•œ ์˜ํ–ฅ์„ ๋ฏธ์น˜์ง€ ์•Š์•˜๋‹ค. 6) ์ด์ง์˜๋„์— ์œ ์˜ํ•œ ์˜ํ–ฅ์„ ๋ฏธ์น˜๋Š” ์š”์ธ์€ ๊ธ์ •์  ์‹ฌ์„ฑ, ๋ถ€์ •์  ์‹ฌ์„ฑ, ๊ต์œก์ˆ˜์ค€, ๊ทผ๋ฌด์—ฐ์ˆ˜, ์‹œ์„ค์œ ํ˜•, ๋Œ๋ด„ ๋Œ€์ƒ์ž์ˆ˜์ด๋ฉฐ, ๊ฐ์ •๋…ธ๋™, ๊ฐ์„ฑ์ง€๋Šฅ, ์ž๊ธฐํšจ๋Šฅ๊ฐ์€ ์œ ์˜ํ•œ ์˜ํ–ฅ์„ ๋ฏธ์น˜์ง€ ์•Š์•˜๋‹ค. 7) ์ง๋ฌด์„ฑ๊ณผ์— ์œ ์˜ํ•œ ์˜ํ–ฅ์„ ๋ฏธ์น˜๋Š” ์š”์ธ์€ ์ž๊ธฐํšจ๋Šฅ๊ฐ, ๊ฐ์„ฑ์ง€๋Šฅ, ์‹œ์„ค์œ ํ˜•, ๋Œ๋ด„ ๋Œ€์ƒ์ž์ˆ˜์ด๋ฉฐ, ๊ฐ์ •๋…ธ๋™, ์„ฑ๊ฒฉ์€ ์œ ์˜ํ•œ ์˜ํ–ฅ์„ ๋ฏธ์น˜์ง€ ์•Š์•˜๋‹ค. ๋ณธ ์—ฐ๊ตฌ์˜ ๊ฒฐ๊ณผ์—์„œ ์š”์–‘๋ณดํ˜ธ์‚ฌ์˜ ๊ฐ์ •๋…ธ๋™์€ ์ง๋ฌด๋งŒ์กฑ์—๋งŒ ์˜ํ–ฅ์„ ๋ฏธ์น˜๋ฉฐ, ๊ฐ์„ฑ์ง€๋Šฅ์€ ์ง๋ฌด๋งŒ์กฑ๊ณผ ์ง๋ฌด์„ฑ๊ณผ์— ์˜ํ–ฅ์„ ๋ฏธ์น˜๋Š” ๊ฒƒ์„ ์•Œ ์ˆ˜ ์žˆ๋‹ค. ๋˜ํ•œ, ์š”์–‘๋ณดํ˜ธ์‚ฌ์˜ ์„ฑ๊ฒฉ์ด ์ง๋ฌด์ŠคํŠธ๋ ˆ์Šค, ์ง๋ฌด์†Œ์ง„, ์ง๋ฌด๋งŒ์กฑ, ์ด์ง์˜๋„์— ์œ ์˜ํ•œ ์˜ํ–ฅ์„ ์ฃผ์—ˆ๋‹ค. ์ด์ฒ˜๋Ÿผ ์š”์–‘๋ณดํ˜ธ์‚ฌ์˜ ์‹ฌ๋ฆฌ์ , ์ •์„œ์  ์š”์ธ์ด ์กฐ์งํšจ๊ณผ์„ฑ์— ๋งŽ์€ ์˜ํ–ฅ์„ ์ฃผ๊ณ  ์žˆ๋Š” ๊ฒƒ์„ ์•Œ ์ˆ˜ ์žˆ์—ˆ๋‹ค. ๋”ฐ๋ผ์„œ ๊ฐ์ •๋…ธ๋™์˜ ์ˆ˜์ค€์„ ์ค„์ด๊ณ  ์กฐ์งํšจ๊ณผ์„ฑ์„ ๋†’์ด๊ธฐ ์œ„ํ•ด์„œ๋Š” ์š”์–‘๋ณดํ˜ธ์‚ฌ๋ฅผ ์œ„ํ•œ ๊ณ ์ถฉ์ƒ๋‹ด์ œ๋„ ๋งˆ๋ จ, ์‹ฌ๋ฆฌ์ƒ๋‹ด์†Œ ์„ค๋ฆฝ, ๊ต์œกํ”„๋กœ๊ทธ๋žจ์˜ ํ™•๋Œ€, ๊ทผ๋กœํ™˜๊ฒฝ๊ฐœ์„  ๋“ฑ ๊ณต์‹์ , ๋น„๊ณต์‹์  ๋…ธ๋ ฅ์ด ํ•„์š”ํ•  ๊ฒƒ์ด๋‹ค.ope
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