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    ์ง๋ฌด๋งŒ์กฑ์˜ ๋งค๊ฐœํšจ๊ณผ๋ฅผ ์ค‘์‹ฌ์œผ๋กœ

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    ํ•™์œ„๋…ผ๋ฌธ(์„์‚ฌ) -- ์„œ์šธ๋Œ€ํ•™๊ต๋Œ€ํ•™์› : ํ–‰์ •๋Œ€ํ•™์› ๊ณต๊ธฐ์—…์ •์ฑ…ํ•™๊ณผ, 2022. 8. ๋ฐ•์ƒ์ธ.๋ณธ ์—ฐ๊ตฌ๋Š” ์ฝ”๋กœ๋‚˜19๋กœ ์ „๊ฒฉ์ ์ด๊ณ  ์ „๋ฉด์ ์œผ๋กœ ์‹ค์‹œ๋œ ์žฌํƒ๊ทผ๋ฌด์˜ ์กฐ์ง ํšจ๊ณผ์„ฑ์„ ํ•œ๊ตญํ–‰์ •์—ฐ๊ตฌ์›์˜ ใ€Œ2020๋…„ ๊ณต์ง์ƒํ™œ ์‹คํƒœ์กฐ์‚ฌใ€ ์ž๋ฃŒ๋ฅผ ํ† ๋Œ€๋กœ ์‹ค์ฆ๋ถ„์„ํ•˜์˜€๋‹ค. ํŠนํžˆ ์žฌํƒ๊ทผ๋ฌด์ œ ์ด์šฉ ๊ฒฝํ—˜ ๋ฐ ์ด์šฉ ๋งŒ์กฑ๋„ ์™€ ์กฐ์ง์‹œ๋ฏผํ–‰๋™๊ณผ์˜ ์ƒ๊ด€๊ด€๊ณ„์—์„œ ์ง๋ฌด๋งŒ์กฑ์ด ๋งค๊ฐœํšจ๊ณผ๋ฅผ ๊ฐ–๋Š”์ง€๋ฅผ Baron & Kenny(1986)๊ฐ€ ์ œ์‹œํ•œ 3๋‹จ๊ณ„ ๋งค๊ฐœํšจ๊ณผ ๊ฒ€์ฆ์„ ํ†ตํ•ด ๋ถ„์„ํ•˜์˜€๋‹ค. ์ด๋Š” ํ–ฅํ›„ ์ฝ”๋กœ๋‚˜19๊ฐ€ ์ข…์‹๋˜๋”๋ผ๋„ ์‚ฌ๋ฌด์‹ค ๊ทผ๋ฌด์™€ ๋ณ‘ํ–‰ํ•˜๋Š” ํ•˜์ด๋ธŒ๋ฆฌ๋“œํ˜• ์žฌํƒ๊ทผ๋ฌด๊ฐ€ ์ผ์ƒํ™”๋  ๊ฒƒ์ด๋ฏ€๋กœ, ๋ณธ ์—ฐ๊ตฌ๋Š” ์žฌํƒ๊ทผ๋ฌด์˜ ์กฐ์ง ํšจ๊ณผ์„ฑ์„ ์‹ค์งˆ์ ์œผ๋กœ ์ œ๊ณ ํ•  ์ˆ˜ ์žˆ๋„๋ก ์กฐ์ง ์ฐจ์›์—์„œ ์‹ค์ฒœ์  ํ•จ์˜๋ฅผ ๋„์ถœํ•˜๋Š” ๋ฐ์— ๊ทธ ๋ชฉ์ ์ด ์žˆ๋‹ค. ๋ณธ ์—ฐ๊ตฌ์˜ ๋ถ„์„๊ฒฐ๊ณผ๋ฅผ ์š”์•ฝํ•˜๋ฉด ๋‹ค์Œ๊ณผ ๊ฐ™๋‹ค. ์žฌํƒ๊ทผ๋ฌด ์ด์šฉ ๊ฒฝํ—˜์„ ๋…๋ฆฝ๋ณ€์ˆ˜๋กœ ์„ค์ •ํ•œ ์˜ ๋ถ„์„ ๊ฒฐ๊ณผ๋Š” ๋‹ค์Œ๊ณผ ๊ฐ™๋‹ค. ์ฒซ์งธ, ์žฌํƒ๊ทผ๋ฌด ์ด์šฉ ๊ฒฝํ—˜๊ณผ ์ง๋ฌด๋งŒ์กฑ์˜ ๊ด€๊ณ„๋Š” ํ†ต๊ณ„์ ์œผ๋กœ ์œ ์˜ํ•˜์ง€ ์•Š์ง€๋งŒ ๋ถ€์ •์ (-)์ธ ๊ด€๊ณ„๊ฐ€ ์žˆ์—ˆ๋‹ค. ์ด๊ฒƒ์€ ์žฌํƒ๊ทผ๋ฌด์˜ ํ™œ์šฉ์ด ๊ณ ๋ฆฝ๊ฐ์„ ์ฆ๊ฐ€์‹œํ‚ค๊ณ , ์žฌํƒ๊ทผ๋ฌด๋กœ ์ธํ•œ ๊ณ ๋ฆฝ๊ฐ์€ ์ŠคํŠธ๋ ˆ์Šค ์š”์ธ์ด ๋˜๋Š” ๋™๋ฃŒ, ์ƒ์‚ฌ์™€ ๊ฐ™์€ ๋Œ€์ธ๊ด€๊ณ„ ๋ฐ ์˜์‚ฌ์†Œํ†ต์˜ ๊ฐ์†Œ๋กœ์„œ ์ง๋ฌด๋งŒ์กฑ๊ณผ ๋ถ€์ ์ธ ๊ด€๊ณ„์— ์žˆ๋Š” ์ง๋ฌด์ŠคํŠธ๋ ˆ์Šค์˜ ์ฆ๊ฐ€๋กœ ์ด์–ด์ ธ ์žฌํƒ๊ทผ๋ฌด ์ด์šฉ ๊ฒฝํ—˜์ด ๋งŽ์„์ˆ˜๋ก ์ง๋ฌด๋งŒ์กฑ์€ ๋‚ฎ์•„์งˆ ๊ฐ€๋Šฅ์„ฑ์ด ์žˆ๋Š” ๊ฒƒ์€ ์•„๋‹Œ์ง€ ์ถ”์ธกํ•  ์ˆ˜ ์žˆ์—ˆ๋‹ค. ๋‘˜์งธ, ์žฌํƒ๊ทผ๋ฌด ์ด์šฉ ๊ฒฝํ—˜๊ณผ ์กฐ์ง์‹œ๋ฏผํ–‰๋™์˜ ๊ด€๊ณ„๊ฐ€ p=0.055 ์ˆ˜์ค€์—์„œ ํ†ต๊ณ„์ ์œผ๋กœ ์œ ์˜ํ•˜์ง€ ์•Š์ง€๋งŒ, ๋ถ€์ •์ (-)์ธ ๊ด€๊ณ„์— ์žˆ์—ˆ๋‹ค. ์žฌํƒ๊ทผ๋ฌด ์ด์šฉ ๊ฒฝํ—˜์ด ๋งŽ์„ ๊ฒฝ์šฐ์— ๊ณ ๋ฆฝ๊ฐ์„ ๋Š๋ผ๋Š” ์ง์›์€ ์ƒ๊ธ‰์ž ๋˜๋Š” ์ฃผ๋ณ€ ๋™๋ฃŒ๋กœ๋ถ€ํ„ฐ ์˜์‚ฌ๊ฒฐ์ •๊ณผ์ •์—์„œ ์กด์ค‘๊ณผ ์˜ˆ์˜, ์ถฉ๋ถ„ํ•œ ์„ค๋ช… ๋“ฑ์„ ๋ฐ›์ง€ ๋ชปํ•œ๋‹ค๊ณ  ์ƒ๊ฐํ•  ์ˆ˜ ์žˆ์„ ๊ฒƒ์ด๋ฉฐ ํ•ด๋‹น ์ง์›์€ ํ˜ธํ˜œ์„ฑ์˜ ์›์น™์— ๊ธฐ๋ฐ˜ํ•œ โ€˜์‚ฌํšŒ๊ตํ™˜์ด๋ก โ€™์— ๋”ฐ๋ฅธ ์กฐ์ง์นœํ™”์ ์ธ ์กฐ์ง์‹œ๋ฏผํ–‰๋™์„ ํ•˜๊ธฐ ์–ด๋ ค์šธ ๊ฐ€๋Šฅ์„ฑ์ด ์žˆ๋Š” ๊ฒƒ์€ ์•„๋‹Œ์ง€ ์ถ”์ธกํ•  ์ˆ˜ ์žˆ์—ˆ๋‹ค. ์žฌํƒ๊ทผ๋ฌด ์ด์šฉ ๋งŒ์กฑ๋„ ๋ฅผ ๋…๋ฆฝ๋ณ€์ˆ˜๋กœ ์„ค์ •ํ•œ ์˜ ๋ถ„์„ ๊ฒฐ๊ณผ๋Š” ๋‹ค์Œ๊ณผ ๊ฐ™๋‹ค. ์ฒซ์งธ, ์žฌํƒ๊ทผ๋ฌด ์ด์šฉ ๋งŒ์กฑ๋„ ๋Š” ์ง๋ฌด๋งŒ์กฑ์— ๊ธ์ •์ (+)์ธ ์˜ํ–ฅ์„ ๋ฏธ์น˜๋Š” ๊ฒƒ์œผ๋กœ ๋ถ„์„๋˜์—ˆ๋‹ค. ์žฌํƒ๊ทผ๋ฌด ์ด์šฉ ๊ฒฝํ—˜๊ณผ ์žฌํƒ๊ทผ๋ฌด ์ด์šฉ ๋งŒ์กฑ๋„ ๋ฅผ ๋ถ„๋ฆฌํ•˜์—ฌ ์žฌํƒ๊ทผ๋ฌด ์ด์šฉ ๋งŒ์กฑ๋„ ๊ฐ€ ๋†’์„์ˆ˜๋ก ์ง๋ฌด๋งŒ์กฑ๋„๋„ ๋†’์•„์ง„๋‹ค๋Š” ๊ฒƒ์„ ์‹ค์ฆํ•œ ๊ฒƒ์ด๋‹ค. ์ด๊ฒƒ์€ ์ง๋ฌด๋งŒ์กฑ๊ณผ ๋ถ€์ •์ (-)์ธ ๊ด€๊ณ„์— ์žˆ๋Š” ๊ณ ๋ฆฝ๊ฐ์ด๋‚˜ ์ง๋ฌด ์ŠคํŠธ๋ ˆ์Šค๋Š” ๋‚ฎ์„ ๊ฒƒ์ด๋ฏ€๋กœ ์žฌํƒ๊ทผ๋ฌด ์ด์šฉ ๋งŒ์กฑ๋„ ๊ฐ€ ๋†’์„์ˆ˜๋ก ์ง๋ฌด๋งŒ์กฑ๋„๋„ ๋†’์•„์ง„๋‹ค๋Š”๊ณ  ์ถ”์ธกํ•  ์ˆ˜ ์žˆ๋‹ค. ๋‘˜์งธ, ์žฌํƒ๊ทผ๋ฌด ์ด์šฉ ๋งŒ์กฑ๋„ ๋Š” ์กฐ์ง์‹œ๋ฏผํ–‰๋™์— ๊ธ์ •์ (+)์ธ ์˜ํ–ฅ์„ ๋ฏธ์น˜๋Š” ๊ฒƒ์œผ๋กœ ๋ถ„์„๋˜์—ˆ๋‹ค. ์žฌํƒ๊ทผ๋ฌด ์ด์šฉ ๋งŒ์กฑ๋„ ๊ฐ€ ๋†’์€ ์ง์›์˜ ๊ฒฝ์šฐ, ๊ณ ๋ฆฝ๊ฐ์€ ์ ์„ ๊ฒƒ์ด๊ณ , ์ƒ๊ธ‰์ž์™€ ์ฃผ๋ณ€ ๋™๋ฃŒ์™€ ์˜์‚ฌ๊ฒฐ์ •๊ณผ์ •์—์„œ ์šฐํ˜ธ์ ์ธ ๊ฐ์ •์„ ๊ฐ€์ง€๊ฒŒ ๋˜๊ณ , ์ด๋Ÿฌํ•œ ์šฐํ˜ธ์ ์ธ ๊ฐ์ •์— ๊ทผ๊ฑฐํ•˜์—ฌ ์ƒ๊ธ‰์ž ๋˜๋Š” ์ฃผ๋ณ€ ๋™๋ฃŒ๋ฅผ ์œ„ํ•œ ์ž๋ฐœ์ ์ด๊ณ  ์กฐ์ง์นœํ™”์ ์ธ ์กฐ์ง์‹œ๋ฏผํ–‰๋™์„ ํ•˜๊ฒŒ ๋  ๊ฒƒ์ด๋ผ๊ณ  ๋…ผ๋ฆฌ์ ์œผ๋กœ ์ถ”์ธกํ•  ์ˆ˜ ์žˆ๋‹ค. ์…‹์งธ, ์žฌํƒ๊ทผ๋ฌด ์ด์šฉ ๋งŒ์กฑ๋„ ์™€ ์กฐ์ง์‹œ๋ฏผํ–‰๋™๊ณผ ๊ด€๊ณ„์—์„œ ์ง๋ฌด๋งŒ์กฑ์ด โ€˜์™„์ „๋งค๊ฐœํšจ๊ณผโ€™๊ฐ€ ์žˆ๋Š” ๊ฒƒ์œผ๋กœ ๋ถ„์„๋˜์—ˆ๋‹ค. ์ด๋Š” ์ง๋ฌด๋งŒ์กฑ์ด ํฌ๋ฉด ํด์ˆ˜๋ก ๊ธ์ •์ ์ด๊ณ  ์ž๋ฐœ์ ์ธ ์กฐ์ง์นœํ™”์  ํ–‰๋™์„ ๋”์šฑ ๋งŽ์ด ์‹คํ–‰ํ•œ๋‹ค๋Š” ๊ฒƒ์„ ๋‚˜ํƒ€๋‚ธ ๊ฒƒ์ด๋‹ค. ๋ถ„์„ ๊ฒฐ๊ณผ๋ฅผ ํ†ตํ•ด ์–ป์„ ์ˆ˜ ์žˆ๋Š” ๋ณธ ์—ฐ๊ตฌ์˜ ์˜์˜ ๋ฐ ์‹œ์‚ฌ์ ์€ ๋‹ค์Œ๊ณผ ๊ฐ™๋‹ค. ์ฒซ์งธ ์žฌํƒ๊ทผ๋ฌด์˜ ํšจ๊ณผ์„ฑ์— ๊ด€ํ•œ ๊ธฐ์กด ์—ฐ๊ตฌ๊ฒฐ๊ณผ๋Š” ์กฐ์ง ์ธก๋ฉด์—์„œ ๊ธ์ •์  ์˜ํ–ฅ์„ ์ž‘์šฉํ•  ์ˆ˜๋„, ๋ถ€์ •์  ์˜ํ–ฅ์„ ์ž‘์šฉํ•  ์ˆ˜๋„ ์žˆ๋Š” ๋“ฑ ์ผ๊ด€๋œ ์—ฐ๊ตฌ๊ฒฐ๊ณผ๊ฐ€ ์—†๋Š” ์ƒํ™ฉ์—์„œ ์žฌํƒ๊ทผ๋ฌด์™€ ์กฐ์ง์‹œ๋ฏผํ–‰๋™๊ณผ์˜ ๊ด€๊ณ„์— ๋Œ€ํ•œ ์—ฐ๊ตฌ๋Š” ๋น„๊ต์  ์ƒˆ๋กœ์šด ์—ฐ๊ตฌ๋Œ€์ƒ์œผ๋กœ์„œ ์‹œ์˜์„ฑ์ด ์žˆ์œผ๋ฉฐ, ์žฌํƒ๊ทผ๋ฌด์˜ ํšจ๊ณผ์„ฑ์ด ์กฐ์ง์— ๋ฏธ์น˜๋Š” ์˜ํ–ฅ์„ 46๊ฐœ ์ค‘์•™ํ–‰์ •๊ธฐ๊ด€ ๋ณธ์ฒญ ์†Œ์† ์ผ๋ฐ˜์ง ๊ณต๋ฌด์›๊ณผ 17๊ฐœ ๊ด‘์—ญ์ž์น˜๋‹จ์ฒด ๋ณธ์ฒญ ์†Œ์† ์ผ๋ฐ˜์ง ๊ณต๋ฌด์› 4,339๋ช…์˜ ๊ณต๋ฌด์›์„ ๋Œ€์ƒ์œผ๋กœ ์‹ค์ฆ๋ถ„์„์„ ํ†ตํ•ด ์ฆ๋ช…ํ•˜์˜€๋‹ค๋Š” ์ ์—์„œ ์˜์˜๋ฅผ ๊ฐ€์ง„๋‹ค. ๋‘˜์งธ, ์žฌํƒ๊ทผ๋ฌด ์ด์šฉ ๊ฒฝํ—˜๊ณผ ์žฌํƒ๊ทผ๋ฌด ์ด์šฉ ๋งŒ์กฑ๋„ ๋ฅผ ๋ถ„๋ฆฌํ•˜์—ฌ ์กฐ์ง์— ๋ฏธ์น˜๋Š” ์˜ํ–ฅ์„ ์‹ค์ฆ์ ์œผ๋กœ ๋ถ„์„ํ•˜์˜€๋‹ค๋Š” ์ ์—์„œ ์ดํ›„ ์žฌํƒ๊ทผ๋ฌด์— ๋Œ€ํ•œ ์—ฐ๊ตฌ์— ์ƒˆ๋กœ์šด ์‹œ๊ฐ์„ ์ œ๊ณตํ•˜์˜€๋‹ค. ์ฆ‰, ์žฌํƒ๊ทผ๋ฌด ์ž์ฒด๊ฐ€ ์กฐ์ง์‹œ๋ฏผํ–‰๋™์„ ๊ฒฐ์ •ํ•œ๋‹ค๊ธฐ๋ณด๋‹ค๋Š” ์กฐ์ง ๊ตฌ์„ฑ์›์˜ ๋‹ค์–‘ํ•œ ์‹ฌ๋ฆฌ์ƒํƒœ์™€ ์žฌํƒ๊ทผ๋ฌด์— ๋Œ€ํ•œ ์ธ์‹์ด ์กฐ์ง์‹œ๋ฏผํ–‰๋™์— ์˜ํ–ฅ์„ ๋ฏธ์นœ๋‹ค๋Š” ๊ตฌ์กฐ์—์„œ ์—ฐ๊ตฌ๋ฅผ ์ง„ํ–‰ํ•ด์•ผ ํ•œ๋‹ค๋Š” ๋ฐฉํ–ฅ์„ฑ์„ ์ œ์‹œํ•œ ์ ์—์„œ ์˜๋ฏธ๊ฐ€ ์žˆ๋‹ค. ์…‹์งธ, ์žฌํƒ๊ทผ๋ฌด ์ด์šฉ์„ ๋…๋ คํ•˜๋Š” ๊ฒƒ๋งŒ์œผ๋กœ๋Š” ์กฐ์งํšจ๊ณผ์„ฑ์„ ์ œ๊ณ  ํ•  ์ˆ˜๋Š” ์—†๊ณ  ์žฌํƒ๊ทผ๋ฌด ์ด์šฉ ๋งŒ์กฑ๋„ ๋ฅผ ์ œ๊ณ ํ•˜๋Š” ๋…ธ๋ ฅ์„ ํ†ตํ•ด์„œ ์กฐ์งํšจ๊ณผ์„ฑ์„ ๋†’์ผ ์ˆ˜ ์žˆ๋‹ค๋Š” ์‹ค์ฒœ์  ๋Œ€์•ˆ์„ ๊ณต๊ณต๋ถ€๋ฌธ ์กฐ์ง๊ด€๋ฆฌ์ž์—๊ฒŒ ์ œ์‹œํ•˜์˜€๋‹ค๋Š” ์ ์—์„œ ๋ณธ ์—ฐ๊ตฌ๋Š” ๊ด€๋ จ ํ›„์†์—ฐ๊ตฌ์˜ ์ข‹์€ ์ž๋ฃŒ๊ฐ€ ๋  ๊ฒƒ์œผ๋กœ ๊ธฐ๋Œ€ํ•œ๋‹ค. ํ•œํŽธ, ๋ณธ ์—ฐ๊ตฌ๋Š” ๋‹ค์Œ๊ณผ ๊ฐ™์€ ํ•œ๊ณ„๋ฅผ ์ง€๋‹Œ๋‹ค. ์ฒซ์งธ, ํ•œ๊ตญํ–‰์ •์—ฐ๊ตฌ์›์˜ ใ€Œ2020๋…„ ๊ณต์ง์ƒํ™œ์‹คํƒœ์กฐ์‚ฌใ€๋ฅผ ๋ถ„์„์— ํ™œ์šฉํ•˜์—ฌ ํ‘œ๋ณธ์˜ ๊ทœ๋ชจ ํ™•๋ณด์™€ ์‹ ๋ขฐ์„ฑ์„ ๋ณด์žฅํ•  ์ˆ˜ ์žˆ์—ˆ์œผ๋‚˜ ๋ณธ ์„ค๋ฌธ์ด ์žฌํƒ๊ทผ๋ฌด์˜ ์—ฌ๋Ÿฌ ์š”์ธ์„ ๋ฐ˜์˜ํ•œ ๊ตฌ์กฐํ™”๋œ ์„ค๋ฌธ์ด ์•„๋‹ˆ๋ผ๋Š” ์ ์ด๋‹ค. ๋‘˜์งธ, ๋ณธ ์—ฐ๊ตฌ๋Š” ํŠน์ •์‹œ์ ์— ๊ตญํ•œ๋œ ์ž๋ฃŒ์ธ ใ€Œ2020๋…„ ๊ณต์ง์ƒํ™œ์‹คํƒœ์กฐ์‚ฌใ€๋ฅผ ํ™œ์šฉํ•˜์˜€๊ธฐ ๋•Œ๋ฌธ์— ์‹œ๊ณ„์—ด์ƒ์˜ ์ธ์‹์˜ ๋ณ€ํ™”๋ฅผ ๋ถ„์„ํ•˜๋Š” ๋ฐ๋Š” ํ•œ๊ณ„๊ฐ€ ์žˆ๋‹ค. ์…‹์งธ, ์žฌํƒ๊ทผ๋ฌด์˜ ์กฐ์ง ํšจ๊ณผ์„ฑ์„ ๊ณต๋ฌด์›์„ ์ค‘์‹ฌ์œผ๋กœ ํ•œ์ •ํ•˜์—ฌ ๋ถ„์„ํ•จ์œผ๋กœ์จ ๋ฏผ๊ฐ„๋ถ„์•ผ์— ์žˆ์–ด ์žฌํƒ๊ทผ๋ฌด์˜ ์กฐ์ง ํšจ๊ณผ์„ฑ์„ ์‚ดํŽด๋ณด์ง€ ๋ชปํ•œ ํ•œ๊ณ„๊ฐ€ ์žˆ๋‹ค. ๋„ท์งธ, ๋ณธ ์—ฐ๊ตฌ๋Š” ์žฌํƒ๊ทผ๋ฌด๋ฅผ ์ง์ ‘ ๊ฒฝํ—˜ํ•˜๋Š” ์ˆ˜ํ˜œ์ž๋ฅผ ๋Œ€์ƒ์œผ๋กœ ์ด๋ฃจ์–ด์กŒ๋‹ค. ๊ทธ๋Ÿฌ๋‚˜ ์žฌํƒ๊ทผ๋ฌด๋ฅผ ์‹œํ–‰ํ•˜๋Š” ๊ด€๋ฆฌ์ž๋ฅผ ๋Œ€์ƒ์œผ๋กœ ํ•œ ์—ฐ๊ตฌ๋„ ์‹ค์‹œ๋  ํ•„์š”๊ฐ€ ์žˆ๋‹ค. ์žฌํƒ๊ทผ๋ฌด๋ฅผ ์‹œํ–‰ํ•˜๋Š” ๊ด€๋ฆฌ์ž์— ๋Œ€ํ•œ ๋ถ„์„์„ ํ†ตํ•ด ํ–ฅํ›„ ์žฌํƒ๊ทผ๋ฌด์ œ๋„์˜ ์•ˆ์ •์ ์ธ ์‹œํ–‰์„ ๊ธฐ๋Œ€ํ•  ์ˆ˜ ์žˆ์„ ๊ฒƒ์ด๋‹ค.This study empirically analyzed the organizational effectiveness of telecommuting, which was conducted in a surprise and full-scale manner due to COVID-19, based on the data of the Korea Institute of Administration's 2020 Public Service Life Survey. In particular, whether job satisfaction has a mediating effect in the correlation between telecommuting system experience and use satisfaction and organizational citizenship behavior were analyzed through the three-step mediating effect verification suggested by Baron & Kenny(1986). This is to derive practical implications at the organizational level to substantially enhance the organizational effectiveness of telecommuting, as hybrid telecommuting in parallel with office work will become common even if COVID-19 ends in the future. The analysis results of this study are summarized as follows. The analysis results of the , which sets the telecommuting experience as an independent variable, are as follows. First, the relationship between telecommuting use experience and job satisfaction was not statistically significant, but there was a negative (-) relationship. This can be inferred whether the use of telecommuting increases isolation, and the sense of isolation due to telecommuting leads to an increase in job stress in negative relationships with job satisfaction as a stressor such as colleagues and bosses. Second, the relationship between telecommuting use experience and organizational citizenship behavior was not statistically significant at the p=0.055 level, but there was a negative (-) relationship. If you have a lot of experience using telecommuting, you may think that employees who feel "isolated" do not receive respect, courtesy, and sufficient explanation in the decision-making process from their superiors or colleagues, and it may be difficult to take organizational-friendly organizational citizenship actions based on the principle of reciprocity. The analysis results of the , which sets the satisfaction level of telework use as an independent variable, are as follows. First, it was found that satisfaction with using telecommuting had a positive (+) effect on job satisfaction. By separating the experience of using telecommuting and the satisfaction of using telecommuting, it was demonstrated that the higher the satisfaction of using telecommuting, the higher the job satisfaction. This will result in a low sense of isolation or job stress in a negative (-) relationship with job satisfaction, so it can be assumed that the higher the satisfaction with using telecommuting, the higher the job satisfaction. Second, it was analyzed that satisfaction with using telecommuting had a positive (+) effect on organizational citizenship behavior. It can be logically inferred that employees with high telecommuting satisfaction will have little sense of isolation, favorable feelings in the decision-making process with superiors and colleagues, and voluntary and organizational-friendly citizenship behavior for superiors or colleagues. Third, it was analyzed that job satisfaction has a 'complete mediating effect' in the relationship between satisfaction with using telecommuting and organizational citizenship behavior. This indicates that the greater the job satisfaction, the more positive and voluntary organizational-friendly behavior is executed. The significance and implications of this study that can be obtained through the analysis results are as follows. First, the existing research results on the effectiveness of telecommuting are relatively new research subjects in the absence of consistent research results, such as positively or negatively affecting the organization. It is also significant in that it proved the effect of telecommuting on the organization through empirical analysis of 4,339 public officials in 46 central administrative agencies' headquarters and 17 metropolitan and provincial headquarters. Second, a new perspective was provided for later research on telecommuting in that the effect on the organization was empirically analyzed by separating the experience of telecommuting and the satisfaction of telecommuting. In other words, it is meaningful in that research on telecommuting should be conducted in a structure that various psychological states and perceptions of telecommuting can affect organizational citizenship behavior rather than telecommuting system itself. Third, this study is expected to be a good data for related follow-up studies in that encouraging the use of telecommuting alone cannot improve organizational effectiveness, and a practical alternative that can improve organizational effectiveness through efforts to improve telecommuting satisfaction. On the other hand, this study has the following limitations. First, the 2020 Public Service Life Survey of the Korea Institute of Administration could be used for analysis to secure the size and reliability of the sample, but this survey was not a structured survey reflecting various factors of telecommuting. Second, since this study used the 2020 Public Service Life Survey, a data limited to a specific point in time, there is a limit to analyzing changes in perception in time series. Third, there is a limitation in not examining the organizational effectiveness of telecommuting in the private sector by analyzing the organizational effectiveness of telecommuting focusing on public officials. Fourth, this study was conducted on beneficiaries who directly experience telecommuting. However, it is also necessary to conduct research on managers who perform telecommuting. Through the analysis of managers who perform telecommuting, stable implementation of the telecommuting system can be expected in the future.์ œ 1 ์žฅ ์„œ ๋ก  1 ์ œ 1 ์ ˆ ์—ฐ๊ตฌ์˜ ๋ฐฐ๊ฒฝ ๋ฐ ๋ชฉ์  1 ์ œ 2 ์ ˆ ์—ฐ๊ตฌ์˜ ๋ฒ”์œ„ ๋ฐ ๋ฐฉ๋ฒ• 4 ์ œ 2 ์žฅ ์ด๋ก ์  ๋ฐฐ๊ฒฝ ๋ฐ ์„ ํ–‰์—ฐ๊ตฌ ๋ถ„์„ 5 ์ œ 1 ์ ˆ ์žฌํƒ๊ทผ๋ฌด์ œ 5 1. ์žฌํƒ๊ทผ๋ฌด์ œ์˜ ๊ฐœ๋… 5 2. ์žฌํƒ๊ทผ๋ฌด์ œ๋ฅผ ํฌํ•จํ•œ ์›๊ฒฉ๊ทผ๋ฌด์ œ์˜ ๋ฒ•์  ๊ทผ๊ฑฐ 7 3. ์žฌํƒ๊ทผ๋ฌด์ œ ๊ด€๋ จ ์„ ํ–‰์—ฐ๊ตฌ 7 ์ œ 2 ์ ˆ ์กฐ์ง์‹œ๋ฏผํ–‰๋™ 17 1. ์กฐ์ง์‹œ๋ฏผํ–‰๋™์˜ ๊ฐœ๋… 17 2. ์กฐ์ง์‹œ๋ฏผํ–‰๋™์˜ ์‹ฌ๋ฆฌ์  ํ˜•์„ฑ์š”์ธ 18 3. ์กฐ์ง์‹œ๋ฏผํ–‰๋™ ๊ด€๋ จ ์„ ํ–‰์—ฐ๊ตฌ 19 ์ œ 3 ์ ˆ ์ง๋ฌด๋งŒ์กฑ 21 1. ์ง๋ฌด๋งŒ์กฑ์˜ ๊ฐœ๋… 21 2. ์ง๋ฌด๋งŒ์กฑ ๊ด€๋ จ ์„ ํ–‰์—ฐ๊ตฌ 22 ์ œ 4 ์ ˆ ์„ ํ–‰์—ฐ๊ตฌ์™€์˜ ์ฐจ๋ณ„์„ฑ 27 ์ œ 3 ์žฅ ์—ฐ๊ตฌ ๋ฐฉ๋ฒ• 28 ์ œ 1 ์ ˆ ์—ฐ๊ตฌ์˜ ๋ถ„์„ํ‹€ 28 1. ์—ฐ๊ตฌ ๋ชจํ˜• 28 2. ์—ฐ๊ตฌ ๊ฐ€์„ค 30 ์ œ 2 ์ ˆ ๋ณ€์ˆ˜์˜ ์กฐ์ž‘์  ์ •์˜ 39 1. ์žฌํƒ๊ทผ๋ฌด(๋…๋ฆฝ๋ณ€์ˆ˜) 39 2. ์กฐ์ง์‹œ๋ฏผํ–‰๋™(์ข…์†๋ณ€์ˆ˜) 40 3. ์ง๋ฌด๋งŒ์กฑ(๋งค๊ฐœ๋ณ€์ˆ˜) 40 4. ํ†ต์ œ๋ณ€์ˆ˜ 41 ์ œ 3 ์ ˆ ์ž๋ฃŒ์ˆ˜์ง‘ ๋ฐ ๋ถ„์„๋ฐฉ๋ฒ• 43 1. ์ž๋ฃŒ์ˆ˜์ง‘ 43 2. ์„ค๋ฌธ๋‚ด์šฉ 45 3. ๋ถ„์„๋ฐฉ๋ฒ• 46 ์ œ 4 ์žฅ ๋ถ„์„ ๊ฒฐ๊ณผ 48 ์ œ 1 ์ ˆ ํ‘œ๋ณธ์˜ ์ธ๊ตฌ์‚ฌํšŒํ•™์  ํŠน์„ฑ 48 ์ œ 2 ์ ˆ ๋ณ€์ˆ˜๋“ค์˜ ๊ธฐ์ดˆํ†ต๊ณ„ 54 1. ๋…๋ฆฝ๋ณ€์ˆ˜์˜ ๊ธฐ์ดˆํ†ต๊ณ„ 54 2. ์ข…์†๋ณ€์ˆ˜์˜ ๊ธฐ์ดˆํ†ต๊ณ„ 55 3. ๋งค๊ฐœ๋ณ€์ˆ˜์˜ ๊ธฐ์ดˆํ†ต๊ณ„ 56 ์ œ 3 ์ ˆ ์š”์ธ๋ถ„์„, ์‹ ๋ขฐ๋„๋ถ„์„, ์ƒ๊ด€๊ด€๊ณ„๋ถ„์„ 56 1. ์š”์ธ๋ถ„์„ 56 2. ์‹ ๋ขฐ๋„๋ถ„์„ 59 3. ์ฃผ์š” ๋ณ€์ˆ˜ ๊ฐ„ ์ƒ๊ด€๊ด€๊ณ„๋ถ„์„ 62 ์ œ 4 ์ ˆ ์ธ๊ตฌ์‚ฌํšŒํ•™์  ์†์„ฑ์— ๋”ฐ๋ฅธ ์ง‘๋‹จ๊ฐ„ ์ฐจ์ด ๋ถ„์„ 64 1. ์ง‘๋‹จ ๊ฐ„ ๋ถ„์„ 64 2. ์ง‘๋‹จ ๊ฐ„ ๋ถ„์„ 68 ์ œ 5 ์ ˆ ์—ฐ๊ตฌ๊ฐ€์„ค ๊ฒ€์ฆ 72 1. ํšŒ๊ท€๋ถ„์„ 73 2. ํšŒ๊ท€๋ถ„์„ 76 ์ œ 5 ์žฅ ๊ฒฐ๋ก  83 ์ œ 1 ์ ˆ ๋ถ„์„๊ฒฐ๊ณผ ์š”์•ฝ ๋ฐ ํ•ด์„ 83 ์ œ 2 ์ ˆ ์—ฐ๊ตฌ์˜ ์˜์˜ ๋ฐ ์‹œ์‚ฌ์  88 ์ œ 3 ์ ˆ ์—ฐ๊ตฌ์˜ ํ•œ๊ณ„ 90 ์ฐธ๊ณ ๋ฌธํ—Œ 92 Abstract 102์„

    Epidemiological study on host animals of tsutsugamushi disease

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    ์˜ํ•™๊ณผ/์„์‚ฌ[ํ•œ๊ธ€] ์ตœ๊ทผ ์šฐ๋ฆฌ๋‚˜๋ผ์—์„œ์˜ ์ญˆ์ญˆ๊ฐ€๋ฌด์‹œ๋ณ‘ ๋ฐœ์ƒ ์–‘์ƒ์„ ๋ณด๋ฉด 1985๋…„์— 64๋ช…, 1986๋…„์— 460๋ช…, 1957๋…„์— 784๋ช…, ๊ทธ๋ฆฌ๊ณ  1995๋…„์— 977๋ช…์œผ๋กœ ๊ณ„์† ์ „๊ตญ์ ์œผ๋กœ ์ฆ๊ฐ€ํ•˜๊ณ  ์žˆ์–ด ๊ทธ ๋Œ€์ฑ…์ด ์‹œ๊ธ‰ํ•œ ์‹ค์ •์ด๋‹ค. ๊ทธ๋Ÿฌ๋‚˜ ๋“ฑ์ค„์ฅ (Apodemus agrarius) ๋ณธ ์งˆ๋ณ‘์˜ ์ˆ™์ฃผ๋™๋ฌผ์ด๋ผ๋Š” ์‚ฌ์‹ค๊ณผ Lepfofrombidium pallidum์ด ๋งค๊ฐœ์ข…์ด๋ผ๋Š” ์‚ฌ์‹ค์„ Jackson ๋“ฑ(1957)์ด ๋ณด๊ณ ํ•œ ์ด๋ž˜ ์•„์ง๊นŒ์ง€ ์ˆ™์ฃผ๋™๋ฌผ๊ณผ ๋งค๊ฐœ์ข…์— ๋Œ€ํ•œ ์—ญํ•™์  ์กฐ์‚ฌ๊ฐ€ ๊ฑฐ์˜ ์ด๋ฃจ์–ด์ ธ์žˆ์ง€ ์•Š๊ธฐ ๋•Œ๋ฌธ์— ์ €์ž๋Š” 1987๋„Œ 7์›”๋ถ€ํ„ฐ 1990๋„Œ 9์›”๊นŒ์ง€ 9๊ฐœ ์ง€์—ญ์—์„œ ์ˆ™์ฃผ๋™๋ฌผ์ธ ๋“ฑ์ค„์ฅ์— ๋Œ€ํ•œ ์—ญํ•™์  ์กฐ์‚ฌ๋ฅผ ์‹ค์‹œํ•œ ๊ฒฐ๊ณผ๋ฅผ ๋‹ค์Œ๊ณผ ๊ฐ™์ด ์š”์•ฝํ•œ๋‹ค. 1. ๋ชจ๋‘ 794๊ฐœ์˜ Sherman์‹œ live trap์„ ์„ค์น˜ํ•˜์—ฌ 111๋งˆ๋ฆฌ์˜ ๋“ค์ฅ๋ฅผ ํฌํšํ•˜์—ฌ trapping rate๋Š” 14.0%์ด์—ˆ๋‹ค. ๊ณ„์ ˆ๋ณ„๋กœ๋Š” ๋ด„, ์—ฌ๋ฆ„, ๊ฐ€์„์— ๊ฐ๊ฐ 11.7%, 11.2%, 12%์ด์—ˆ๊ณ  ๊ฒจ์šธ์—๋Š” 20% ๊ฐ€์žฅ ๋†’์•˜๋‹ค. 2. ์กฐ์‚ฌ๊ธฐ๊ฐ„์ค‘ ์ด 111๋งˆ๋ฆฌ์˜ ๋“ค์ฅ๋ฅผ ์ฑ„์ง‘ํ•˜์˜€๋Š”๋ฐ ๊ทธ ์ค‘ 103๋งˆ๋ฆฌ๊ฐ€ ๋“ฑ์ค„์ฅ (Apodemus agrarius)๋กœ 92.8%๋กœ ์šฐ์ ์ข…์ด์—ˆ๊ณ  ๋•ƒ์ฅ (Crocidura laisura)๊ฐ€ 7๋งˆ๋ฆฌ๋กœ 6.3% ์ด์—ˆ์œผ๋ฉฐ ๊ฐˆ๋ฐญ์ฅ (Microtus fortis)๋Š” 1๋งˆ๋ฆฌ๊ฐ€ ์ฑ„์ง‘๋˜์–ด 0.9%์— ๋ถˆ๊ณผํ•˜์˜€๋‹ค. 3. ๋“ค์ฅ์˜ ํ„ธ์ง„๋“œ๊ธฐ ๊ธฐ์ƒ๋ฅ ์„ ๋ณด๋ฉด ๋“ฑ์ค„์ฅ์˜ ๊ฒฝ์šฐ 103๋งˆ๋ฆฌ์ค‘ 84๋งˆ๋ฆฌ๋กœ 81.6% ์ด์—ˆ๊ณ , ๋•ƒ์ฅ์˜ ๊ฒฝ์šฐ 7๋งˆ๋ฆฌ์ค‘ 2๋งˆ๋ฆฌ๋กœ 28.6% ์—ˆ๋‹ค. Chiggr index(์ฅ ํ•œ๋งˆ๋ฆฌ๋‹น ํ„ธ์ง„๋“œ๊ธฐ ์œ ์ถฉ ๊ธฐ์ƒ์ˆ˜)๋Š” ๋“ฑ์ค„์ฅ์˜ ๊ฒฝ์šฐ 43.0 ๊ฐœ์ฒด์˜€๊ณ  ๋•ƒ์ฅ๋Š” 0.9 ๊ฐœ์ฒด์˜€๋‹ค. 4. ๋“ฑ์ค„์ฅ์˜ Rickettsia tsutsugamushi ํ•ญ์ฒด ์•™์„ฑ๋ฅ ์„ ๋ณด๋ฉด ํŽ‘๊ท  41.8%์˜€๋Š”๋ฐ, ๊ณ ์–‘๊ตฐ ๋„๋ž˜ 5๋ฆฌ์—์„œ 54.9%, ๊ณ ์–‘๊ตฐ ๊ณ ์–‘๋ฆฌ 20%, ๊ณ ์–‘๊ตฐ ์›ํฅ 2๋ฆฌ 28.6%, ์ฒญ์›๊ตฐ ์‹œ๋ชฉ๋ฆฌ 40%์ด์—ˆ๊ณ  ๊ณ ์–‘๊ตฐ ํ–‰์‹  1๋ฆฌ, ํŒŒ์ฃผ๊ตฐ ๊ด‘ํƒ„ 3๋ฆฌ, ์ถฉ๋‚จ ์˜ˆ์‚ฐ๊ตฐ ๋•์‚ฐ๋ฆฌ ๋“ฑ ์ง€์—ญ์—์„œ๋Š” ๋ชจ๋‘ 0% ์˜€๋‹ค. ๋•ƒ์ฅ์˜ ๊ฒฝ์šฐ๋Š” 7๋งˆ๋ฆฌ ๋ชจ๋‘ ์Œ์„ฑ์ด์—ˆ๋‹ค. 5. ๋™์ผ ์žฅ์†Œ์—์„œ์˜ ๋“ฑ์ค„์ฅ์˜ R. tsutsugamushi ํ•ญ์ฒด ์–‘์„ฑ๋ฅ ์˜ ๊ณ„์ ˆ์  ๋ณ€๋™์€ 11์›”๋ถ€ํ„ฐ 3์›”๊นŒ์ง€๋Š” ํ‰๊ท  75.8%์ด์—ˆ๋Š”๋ฐ 4์›”์—๋Š” 30.3%๋กœ ๊ฐ์†Œํ•˜์˜€๊ณ  5์›”๋ถ€ํ„ฐ 8์›”์— ๊ฑธ์ณ ํ‰๊ท  13.3%๋กœ ํฌ๊ฒŒ ๊ฐ์†Œํ•˜์˜€๋‹ค. 6. 94๋งˆ๋ฆฌ์˜ ๋“ฑ์ค„์ฅ๋ฅผ IFA๋กœ ๊ด€์ฐฐํ•œ ๊ฒฐ๊ณผ R. tsutsugamushi ํ•ญ์› ์–‘์„ฑ์ˆ˜๋Š” 3๊ฐœ์ฒด๋กœ ๋ฒ™์›์ฒด์ธ R. tsutsugamushi ํ•ญ์› ๊ฒ€์ถœ๋ฅ  3.2%์ด์—ˆ๋‹ค. [์˜๋ฌธ] Human cases of tsutsugamushi disease have been increasing in Korea since 1985, being reported 64 cases in 1985, 460 cases in 1986, 784 cases in 1987 and 977 cases in 1988, so that preventive and/or control measures against chi s rickettsial disease are urgently required. Since Jackson et al (1957) reported that the etiological agent, Rickettsia tsutsugamushi was isolated from the striped field mouse (Apodemus agrarius) collected in Korea, epidemiological studies of host animals have not been carried out, except Shein et al.(1989) and Lee et al. (1990). Therefore, the author studies some epidemiological aspects on host animals of tsutsugamushi disease in Korea duping the period of July 1989 - September 1990, and the results are sumlarized as follows. 1. Among total 111 rodents collected during the study period, A.agrarius was 103(92.8% of the total), Crociodura was 7(6.3%) and Microius Fortis was 1(0.9%). 2. As 111 rodents were collected from total 794 Sherman 1 live traps set at 9 different localities, the trapping rate was 14.0% in avepage. The trapping rate was the highest in winter, showing 20% of the trapping rate, whereas 11.7%, 11.2% and 12% in spring, summer and autumn respectively. 3. Out of 103 tsutsugamushi collected, 84 were infested (parasitized) by chigger mites shoring 81.6% of the infestation rate. In case of Crociodura laisusura, the infestation rate was 28.0% (2 out of 7). The chigger index (average number of chigger mites infested per louse) of A.agrarius was 43.0 and that of Crociodura laisusura was 1. 4. The antibody positive rate of A.agrarius serum to R.tsutsugamushi antigen was 54.9% at Doral 5-ri, Goyang-gun, 20% at Goyang-ri, Goyang-gun, 28.6% at Weonhung 2-ri, Goyang-gun, 40% at Simog-ri, Cheongweon-gun, and all negative at Haingsin 1-rl, Goyang-gun, Gwangtan 3-ri, Paju-gun and Deogsan-rl, Yesan-gun, total average of which was 41.8%. Serum of 7 Crociodura laisusura showed all negative. The seasonal change of the antibody positive rate at Dorai 5-ri, Goyang-gun was 75.8% in average during the period of November-March, decreased to 30.3% in April and further decreased to 13.3% in average during the period of May - August. Among 33 antibody positives 31 were Karp strain and 2 were Gilliam. 5. R.tsutsugamshi antigen was isolated from 3 A.agrarius out of 94 individuals tested, showing 3.2% of the infection rate.restrictio

    (The) role of macrophage in the process of experimental Acanthamoeba meningoencephalitis in mice

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    ์˜ํ•™๊ณผ/๋ฐ•์‚ฌ[ํ•œ๊ธ€] ์ž์œ ์ƒํ™œ์•„๋ฉ”๋ฐ”์ธ Acanthamoeba culbertsoni๋Š” ์ธ์ฒด์™€ ์‹คํ—˜๋™๋ฌผ์— ์น˜๋ช…์ ์ธ ์ถœํ˜ˆ์„ฑ ๊ดด์‚ฌ์„ฑ ์ˆ˜๋ง‰๋‡Œ์—ผ์„ ์ผ์œผํ‚ค๋Š” ๊ฒƒ์œผ๋กœ ๋ฐํ˜€๊ฒผ๋‹ค. ๋ณธ ์—ฐ๊ตฌ๋Š” ๋งˆ์šฐ์Šค์—์‹ A. culbertsoni ์— ์˜ํ•œ ์‹คํ—˜์  ์ˆ˜๋ง‰๋‡Œ์—ผ ๋ฐœ์ƒ์‹œ ์ผ์–ด๋‚˜๋Š” ์„ธํฌ๋งค๊ฐœ์„ฑ ๋ฉด์—ญ์˜ ์ผํ™˜์œผ๋กœ ๋Œ€์‹์„ธํฌ์˜ ์—ญํ• ์„ ๊ด€์ฐฐํ•˜๊ธฐ ์œ„ํ•ด ์‹ค์‹œํ•˜์˜€๋‹ค. CSH/HeJ ๋งˆ์šฐ์Šค์— CGV ๋ฐฐ์ง€์—์„œ ๋ฐฐ์–‘๋œ A. culbertsoni ์˜์–‘ํ˜• 3ร—10**5 ๊ฐœ๋ฅผ ๋น„๊ฐ•์— ๋–จ์–ด๋œจ๋ ค ์ ‘์ข…ํ•˜์˜€๋‹ค. ๋งˆ์šฐ์Šค ๋ณต๊ฐ•๋‚ด๋กœ 10% fetal calf serum(FCS)์ด ํ•จ์œ ๋œ Hank's balanced salt solution์„ ์ฃผ์ž…ํ•˜์—ฌ ๋Œ€์‹์„ธํฌ๋ฅผ ์ฑ„์ทจํ•˜๊ณ  10% FCS๊ฐ€ ํ•จ์œ ๋œ RPMI 1640์œผ๋กœ ๋ฐฐ ์–‘ํ•˜์˜€์œผ๋ฉฐ ๋Œ€์‹์„ธํฌ ์–ต์ œ์ œ์ธ silica(์ง๊ฒฝ 0.5ฮผm)๋Š” ๋งˆ์šฐ์Šค ๋ณต๊ฐ•๋‚ด๋กœ ํˆฌ์—ฌํ•˜์˜€๋‹ค. Toxoplasma gondii tachyzoites ๋Š” 56โ„ƒ์—์„œ 15๋ถ„๊ฐ„ ์ฒ˜๋ฆฌํ•˜์—ฌ ์ฃฝ์ธ ํ›„ ๋Œ€์‹์„ธํฌ์˜ ์‹œํ—˜๊ด€๋‚ด ํƒ์‹๋Šฅ ๊ด€์ฐฉ์— ์‚ฌ์šฉํ•˜์˜€๋‹ค. ๋ฐฐ์–‘๊ธฐ์—์„œ ๋Œ€์‹์„ธํฌ์˜ A. culbertsoni ์— ๋Œ€ํ•œ ์‚ดํ•ด๋Šฅ์„ ๊ด€์ฐฐํ•˜๊ธฐ ์œ„ํ•ด 10% FCSํ•จ์œ  RPMI I640 ๋ฐฐ์ง€์—์„œ 2์‹œ๊ฐ„ ๋™์•ˆ ํ˜ผํ•ฉ ๋ฐฐ์–‘ํ•˜์˜€๋‹ค. ๋งˆ์šฐ์Šค ํ˜ˆ์ฒญ๋‚ด interleukin-1ฮฒ๋†๋„๋Š” anti-murine IL- 1ฮฒ๋ฅผ ์‚ฌ์šฉํ•˜์—ฌ ํšจ์†Œํ‘œ์‹๋ฉด์—ญ๊ฒ€์‚ฌ๋ฒ•(ELISA)์œผ๋กœ ์ธก์ •ํ•˜์˜€๋‹ค. A. culbertsoni๋ฅผ ์ ‘์ข…ํ•œ ๊ตฐ๊ณผ, ์•„๋ฉ”๋ฐ”๋ฅผ ์ ‘์ข…ํ•˜๊ณ  ๋ณต๊ฐ•๋‚ด๋กœ silica 0.5mg์„ ํˆฌ์—ฌํ•œ ๊ตฐ์—์„œ ์‚ฌ๋ง๋ฅ ์ด ๊ฐ๊ฐ 10%์™€ 60%๋กœ ์˜์˜์žˆ๋Š” ์ฐจ์ด๋ฅผ ๋ณด์˜€๋‹ค(p<0.05), ์ด ๋•Œ ์‚ฌ๋งํ•œ ๋งˆ์šฐ์Šค์—์„œ ์ˆ˜๋ง‰๋‡Œ์—ผ์ด ๋ฐœ์ƒ๋˜์—ˆ์Œ์ด ๊ด€์ฐฐ๋˜์—ˆ๊ณ  ๋‡Œ์กฐ์ง์œผ๋กœ๋ถ€ํ„ฐ A. culbertsoni ์˜์–‘ํ˜•์ด ๊ฒ€์ถœ๋˜์—ˆ๋‹ค. ์•„๋ฉ”๋ฐ”๋ฅผ ์ ‘์ข…์‹œํ‚จ ๊ตฐ๊ณผ ์•„๋ฉ”๋ฐ”๋ฅผ ์ ‘์ข…ํ•˜๊ณ  silica๋ฅผ ํˆฌ์—ฌํ•œ ๊ตฐ์—์„œ ๊ฐ๊ฐ 1,2,3,5์ผ์— Toxoplasma gondii tachyzoites์— ๋Œ€ํ•œ ๋ณต๊ฐ• ๋Œ€์‹์„ธํฌ์˜ ํƒ์‹๋Šฅ์„ ๊ด€์ฐฐํ•˜์˜€๋Š”๋ฐ, ์•„๋ฉ”๋ฐ”๋งŒ ์ ‘์ข…์‹œํ‚จ ๊ตฐ์€ ๋‚ ์งœ๋ณ„๋กœ 10%, 28.6%, 18.0%, 25.2%์˜ ํƒ์‹๋Šฅ์„ ๋ณด์ธ ๋ฐ˜๋ฉด ์•„๋ฉ”๋ฐ”๋ฅผ ์ ‘์ข…ํ•˜๊ณ  silica๋ฅผ ํˆฌ์—ฌํ•œ ๊ตฐ์—์„œ๋Š” 3์ผ์งธ๊นŒ์ง€ 3%์ดํ•˜์˜ ํ˜„์ €ํ•œ ํƒ์‹๋Šฅ ์ €ํ•˜๋ฅผ ๋ณด์˜€๊ณ (p<0.01) 5์ผ์งธ์—๋Š” ์•„๋ฉ”๋ฐ” ์ ‘์ข…๊ตฐ๊ณผ ๋น„์Šทํ•œ 24.7%์˜ ํƒ์‹๋Šฅ์„ ๋ณด์—ฌ ๋Œ€์‹์„ธํฌ์˜ ๊ธฐ๋Šฅ์ด ํšŒ๋ณต๋˜์–ด ๊ฐ€๋Š” ๊ฒฝํ–ฅ์„ ๋ณด์˜€๋‹ค. A. culbertsoni ์— ๋Œ€ํ•œ ๋Œ€์‹์„ธํฌ์˜ ์‚ดํ•ด๋Šฅ์— ๊ด€ํ•œ ๋ฐฐ์–‘๊ธฐ๋‚ด ๊ด€์ฐฐ์—์„œ Silica๋ฅผ ๋ณต๊ฐ•๋‚ด๋กœ ํˆฌ์—ฌํ•œ ๋งˆ์šฐ์Šค์—์„œ ๋ถ„๋ฆฌํ•œ ๋Œ€์‹์„ธํฌ๊ฐ€ ๋Œ€์กฐ๊ตฐ์—์„œ ๋ถ„๋ฆฌํ•œ ๋Œ€์‹์„ธํฌ์— ๋น„ํ•ด ์˜์˜์žˆ๋Š” ์‚ดํ•ด๋Šฅ ์ €ํ•˜๋ฅผ ๋ณด์˜€์œผ๋ฉฐ, ๋ฐฐ์–‘๊ธฐ๋‚ด์—์„œ ๋‹ค์‹œ silica๋ฅผ ์ฒ˜๋ฆฌํ•œ ๋Œ€์‹์„ธํฌ๊ตฐ๋„ ๋Œ€์กฐ๊ตฐ์˜ ๋Œ€์‹์„ธํฌ์— ๋น„ํ•˜์—ฌ ์‚ดํ•ด๋Šฅ์ด ์–ต์ œ๋˜์—ˆ์Œ์ด ๊ด€์ฐฐ๋˜์—ˆ์œผ๋‚˜ ์ด๋Ÿฌํ•œ ํ˜„์ƒ์€ ๋ฐฐ์–‘๊ธฐ๋‚ด์—์„œ silica์ฒ˜๋ฆฌ์— ์˜ํ•œ ๋Œ€์‹์„ธํฌ์˜ ๊ธฐ๋Šฅ ์ €ํ•˜ํšจ๊ณผ๋ณด๋‹ค silica๋ฅผ ๋ณต๊ฐ•๋‚ด๋กœ ํˆฌ์—ฌํ•œ ํ›„ ๋ถ„๋ฆฌํ•œ ๋Œ€์‹์„ธํฌ์™ธ ๊ธฐ๋Šฅ ์ €ํ•˜๊ฐ€ ์‚ดํ•ด๋Šฅ ๊ฐ์†Œ์— ๋” ์˜๋ฏธ์žˆ๋Š” ์˜ํ–ฅ์„ ๋ฏธ์น˜๋Š” ๊ฒƒ์œผ๋กœ ๊ด€์ฐฐ๋˜์—ˆ๋‹ค(p<0.01). ์•„๋ฉ”๋ฐ” ์ ‘์ข… 1์ผ์งธ์™€ 2์ผ์งธ ๋งˆ์šฐ์Šค ํ˜ˆ์ฒญ๋‚ด์˜Interleukin-1ฮฒ(IL- 1ฮฒ) ๋†๋„๋Š” ์•„๋ฉ”๋ฐ” ์ ‘์ข…๊ตฐ์ด๋‚˜ ๋Œ€์กฐ๊ตฐ๋ณด๋‹ค ์•„๋ฉ”๋ฐ” ์ ‘์ข…ํ•˜๊ณ  silica๋ฅผ ํˆฌ์—ฌํ•œ๊ตฐ์—์„œ ๋‚ฎ๊ฒŒ ๋‚˜ํƒ€๋‚˜ ์˜์˜์žˆ๋Š” ์ฐจ์ด๋ฅผ ๋ณผ ์ˆ˜ ์žˆ์—ˆ๋‹ค. ์ด์ƒ์˜ ๊ฒฐ๊ณผ๋ฅผ ์ข…ํ•ฉํ•ด ๋ณผ ๋•Œ A. culbertsoni์— ์˜ํ•œ ์‹คํ—˜์  ์ˆ˜๋ง‰๋‡Œ์—ผ ์œ ๋ฐœ์‹œ A. culbertsoni๋ฅผ ์ ‘์ข…ํ•˜๊ณ  ๋Œ€์‹์„ธํฌ ์–ต์ œ์ œ์ธ Silica๋ฅผ ํˆฌ์—ฌํ–ˆ์„ ๋•Œ ๋งˆ์šฐ์Šค์˜ ์‚ฌ๋ง๋ฅ ์ด ์ค‘๊ฐ€ํ•˜๊ณ , ๋ฐฐ์–‘๊ธฐ๋‚ด์—์„œ ๋ณต๊ฐ• ๋Œ€์‹์„ธํฌ์˜ T.gondii์— ๋Œ€ํ•œ ํƒ์‹๋Šฅ๊ณผ A. culbertsoni ์˜์–‘ํ˜•์— ๋Œ€ํ•œ ์‚ดํ•ด๋Šฅ์ด ๊ฐ์†Œํ•˜๋ฉฐ, ๋งˆ์šฐ์Šค ํ˜ˆ์•ก๋‚ด์˜ IL-1ฮฒ์˜ ๋†๋„๊ฐ€ ํ˜„์ €ํ•˜๊ฒŒ ๋–จ์–ด์ง€๋Š” ๊ฒƒ์œผ๋กœ ๋ณด์•„, ๋Œ€์‹์„ธํฌ๊ฐ€ A. culbertsoni ๊ฐ์—ผ์— ๋Œ€ํ•œ ๋ฐฉ์–ด์ž‘์šฉ์— ์ค‘์š”ํ•œ ์—ญํ• ์„ ํ•˜๋Š” ๊ฒƒ์œผ๋กœ ์‚ฌ๋ฃŒ๋œ๋‹ค [์˜๋ฌธ] Acanthamoeba culbertsoni is a kind of free-living amoeba. It can cause a fatal meningoence-phalitis in human and experimental animals and destroys brain tissues by causing hemorrhagic necrosis. The role of macrophages in the Process of experimentally induced meningoencephalitis caused by A. culbertsoni in C3H/HeJ mice was observed. In one group, mice were infected with A. culbertsoni intranasal inoculation of A. culbertsoni trophogoites(3ร—17**5). The mortality was 10% in A. culbertsoni infected group. In another group of mice injected intraperitoneally with mocrophage function inhibitor, silica (7.5 m9), the mortality increased to 60% after inoculation of A. culbertsoni. Clinical signs of meningoencephalitis were observed and A. culbertsoni trophozoites were found from the brain tissues of all dead mice by staining with HE (Hematoxilin-eosin). Peritoneal macrophages were separated from all groups respectively at day 1, 2, 3 and 5, and then incubated invitro with CGV media. After incubation of the rnacrophages in Petridishes for 3 days, 2ร—10**7 of killed(heat treated) Toxoplasma tachyzoits were added to all petridishes. The phagocytic in-dices of each macrophages were mesured after 74 hours of co-cultivation. The phagocytic indices of A. culbertsoni infected group were 10%, 28.6%, 15.0% and 25.7% respectively on day 1, 2, 3 and 5 after infection. The phagocytic indices of macrophages from silica injected group were under 3%, in samples separated frown the 1st to the 3rd day, and afterwards the phagocytic index gradually increased to 24.7%, which was similar to that of the A. culbertsoni only infected group. The interleukin-1ฮฒ(IL-1ฮฒ) levels of serums which were separated from the hearts of two mice on the1st and the 2nd day after A. culbertsoni infection were measured by means of ELISA method. The level of serum IL-lฮฒ was highest in A. culbertsoni infected group and lowest in the silica injected group after A. culbertsoni infection, both at serum dilutions of 1'320 and I :640. The amoebicidal activities of mice peritoneal macrophages were measured in vitro examination. The amoebicidal activity of macrophages from the silica injected group was significantly lower than that of the macrophages from control groups. In vitro treatment of silica had lower effect on the reduction of the amoebicidal activity of macrophages. According to the in vitro treatment of silica had more significant effect than in vitro treatment in the reduction of the amoebicidal activity of macrophages. According to the results of the above observations, macrophages were shown to play a significant defensive role in the process of experimentally induced Acanthamoeba menigoencephalitis.restrictio

    Polymer ion exchange membrane fuel cell ion exchange membrane manufacturing apparatus and method

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    ๋ณธ ๋ฐœ๋ช…์€ ๊ณ ๋ถ„์ž ์ด์˜จ๊ตํ™˜๋ง‰ ์—ฐ๋ฃŒ์ „์ง€์˜ ์ด์˜จ๊ตํ™˜๋ง‰์„ ์ œ์กฐํ•˜๋Š” ์žฅ์น˜ ๋ฐ ์ด์˜จ๊ตํ™˜๋ง‰ ์ œ์กฐ๋ฐฉ๋ฒ•์— ๋Œ€ํ•œ ๊ฒƒ์œผ๋กœ์„œ, ๋ฐฉํ–ฅ์กฑ ํƒ„ํ™”์ˆ˜์†Œ ๊ณ ๋ถ„์ž ์šฉ์•ก์ด ๋‹ด์ง€๋˜๊ณ , ๋‹ค๊ณต์„ฑ ๊ธฐ์žฌ๊ฐ€ ๋ฐฐ์น˜๋˜๋Š” ํ•จ์นจ์กฐ ๋ฐ ์ „๊ธฐ์žฅ์„ ๋ฐœ์ƒ์‹œํ‚ค๋Š” ์ „๊ธฐ์žฅ ๋ฐœ์ƒ๊ธฐ๋ฅผ ๊ตฌ๋น„ํ•˜๊ณ , ์ƒ๊ธฐ ์ „๊ธฐ์žฅ ๋ฐœ์ƒ๊ธฐ๋Š” ์Œ๊ทน์ด ํ•จ์นจ์กฐ์™€ ์ „๊ธฐ์ ์œผ๋กœ ์—ฐ๊ฒฐ๋˜๊ณ , ์–‘๊ทน์ด ๋‹ค๊ณต์„ฑ ๊ธฐ์žฌ์™€ ์ „๊ธฐ์ ์œผ๋กœ ์—ฐ๊ฒฐ๋˜์–ด ์ „๊ธฐ์žฅ์„ ๋ฐœ์ƒ์‹œ์ผœ ์ƒ๊ธฐ ๋‹ค๊ณต์„ฑ ๊ธฐ์žฌ์— ๋ฐฉํ–ฅ์กฑ ํƒ„ํ™”์ˆ˜์†Œ๊ณ„ ๊ณ ๋ถ„์ž ์šฉ์•ก์„ ํ•จ์นจ์‹œํ‚ค๋Š” ์ „๊ธฐ์žฅ์„ ์ด์šฉํ•œ ์ด์˜จ๊ตํ™˜๊ณ ๋ถ„์ž ์—ฐ๋ฃŒ์ „์ง€์˜ ์ด์˜จ๊ตํ™˜๋ง‰ ์ œ์กฐ ์žฅ์น˜ ๋ฐ ์ƒ๊ธฐ ์žฅ์น˜๋ฅผ ์ด์šฉํ•˜์—ฌ ๊ณ ๋ถ„์ž ์ด์˜จ๊ตํ™˜๋ง‰ ์—ฐ๋ฃŒ์ „์ง€์˜ ์ด์˜จ๊ตํ™˜๋ง‰์„ ์ œ์กฐํ•˜๋Š” ๋ฐฉ๋ฒ•์„ ์ œ๊ณตํ•œ๋‹ค
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