100 research outputs found
ν λ Ό κ°κΎΈκΈ° νλμ΄ λμ μ΄λ±νμ μνμμμ λ―ΈμΉλ μν₯
νμλ
Όλ¬Έ (μμ¬)-- μμΈλνκ΅ λνμ : μ¬λ²λν νλκ³Όμ νκ²½κ΅μ‘μ 곡, 2018. 2. μ΄μμ£Ό.νλ μ¬νκ° μ²ν μνμ μκΈ°λ νκ²½κ³Ό μ¬ν, κ·Έλ¦¬κ³ λ¬Έν λ±μ΄ μλ‘ λ°μ ν κ΄κ³λ₯Ό λ§Ίκ³ μμ΄ λΆλΆμ μΈ μ κ·Ό λ°©λ²μΌλ‘λ ν΄κ²°μ΄ μ΄λ ΅λ€λ νΉμ±μ΄ μλ€. λ°λΌμ μμ°κ³μ μ΄ν΄λ₯Ό λ°μ΄λμ΄ μ¬νβ€λ¬Ένμ μΈ μΈ‘λ©΄μ ν΅ν©μ μΌλ‘ μ κ·Όνλ €λ λ³νκ° μꡬλκ³ μμΌλ©° μ΄λ¬ν μ κ·Όλ²μ μ ν©ν κ΅μ‘곡κ°μΌλ‘ μμ°κ³Ό λ¬Έν, κ·Έλ¦¬κ³ κ΅μ‘μ ν΅ν©ν μ μλ λ
Ό μνκ³κ° μ£Όλͺ© λ°κ³ μλ€.
μ΄ μ°κ΅¬μμλ λμ μ΄λ±νμμ λμμΌλ‘ κ΅λ΄ 체ννμ΅μ₯(ν
λ
Ό)μ νμ©ν ν
λ
Ό κ°κΎΈκΈ° νλ‘κ·Έλ¨μ μ€μνκ³ , μ€μ λ
Όμ μ νκΈ° μ΄λ €μ΄ λμ μ΄λ±νμμκ² λ―ΈμΉλ μν₯μ μμ보μλ€. μ΄λ₯Ό ν΅ν΄ ν
λ
Όμ νμ©ν μν 체ν νλ‘κ·Έλ¨μ΄ μνκ΅μ‘μμ κ°μ§λ μλ―Έλ₯Ό νμΈνμλ€.
λ¨Όμ ν
λ
Όμ νμ©ν μν 체ν νλ‘κ·Έλ¨μ μ΄μνκΈ° μνμ¬ μνκ΅μ‘μ λν λ¬Ένμ μ΄ν΄λ³΄κ³ , μνμμμ μλ―Έμ ꡬμ±μμλ₯Ό μ μνμλ€. κΈ°μ‘΄μ λ
Όμλ₯Ό μ’
ν©ν΄ λ³Ό λ μνμμμ μνκ³μ λν μ΄ν΄λ₯Ό λ°νμΌλ‘ μ§μ κ°λ₯ν μΆμ μ΄μκ°λ λ₯λ ₯μ΄λ©°, μνμμμ ꡬμ±μμμλ μνμ μ§μ, μνμ κ°μμ±, κ·Έλ¦¬κ³ μνμ€μ¬μ νλκ° μλ€. μνμμμ μΈ μμμ νλ‘κ·Έλ¨ κ΅¬μ±μ λ°μνκ³ , λ
Ό μνκ³λ₯Ό νμ©ν κΈ°μ‘΄μ μ°κ΅¬λ₯Ό λΆμνμ¬ νλ‘κ·Έλ¨ κ°λ°μ νμν μ€κ±°λ₯Ό λ§λ ¨νμλ€.
μ΄λ₯Ό λ°νμΌλ‘ 2015 κ°μ μ΄λ±νκ΅ 3νλ
κ³Όνκ³Ό κ΅μ‘κ³Όμ λ° μ°½μμ 체ννλμ ν΅ν©νμ¬ μ΄ 12μ°¨μμ ν
λ
Ό κ°κΎΈκΈ° νλ‘κ·Έλ¨μ ꡬμνμλ€. 2015 κ°μ μ΄λ±νκ΅ 3νλ
κ³Όνκ³Ό κ΅μ‘κ³Όμ μμ μν κ΄λ ¨ λ΄μ©μ μΆμΆνκ³ , μνμμμ κΈ°λ₯Ό μ μλ μ°½μμ 체ννλμ μ μ νμ¬ κ΅κ³Όμ μ°½μμ 체ννλμ ν΅ν©νμ¬ μ¬κ΅¬μ±νμλ€. 2015 κ°μ μ΄λ±νκ΅ 3νλ
κ³Όνκ³Ό κ΅μ‘κ³Όμ μ ν΄λΉνλ λλ¬Όμ νμ΄μ΄, λλ¬Όμ μν λ¨μμ ν΅ν©νμ¬ μ΄μν¨μΌλ‘μ¨ μμ
μμλ₯Ό ν보νκ³ , μ°½μμ 체ννλκ³Ό κ΅κ³Ό μμ
μ ν΅ν©νμ¬ κ΅μ‘νλμ΄ μλ‘ μ°μμ±μ κ°μ§λλ‘ νμλ€.
μ€ν μ°κ΅¬μμλ μ€μ λ
Όμ μ νκΈ° μ΄λ €μ΄ λμ μ΄λ±νμμκ² μ μ©νμ¬ κ·Έ ν¨κ³Όλ₯Ό μ΄ν΄λ³΄μμΌλ©° μμΈμ κ°λ¨κ΅¬ μμ¬μ μ΄λ±νκ΅ 3νλ
νμ 93λͺ
μ λμμΌλ‘ νμλ€. κΈ°μ‘΄μ μ°κ΅¬μ λ¬λ¦¬ μνμμμ λ―ΈμΉλ μν₯μ μμ보기 μν λ°©λ²μΌλ‘ κ·Έλ¦Ό 그리기λ₯Ό νμ©νμ¬ νλ‘κ·Έλ¨ μ κ³Ό ν, μνμμμ λ―ΈμΉλ μν₯μ νμΈν¨μΌλ‘μ¨ νμλ€μ κ·Έλ¦Όμ ν΅ν΄ λ€μν λ°μμ μμ§ν μ μμκ³ , κ·Έλ¦Όλ§μΌλ‘ μ΄ν΄ν μ μλ λΆλΆμ μΆκ° μ§λ¬Έκ³Ό λ©΄λ΄μ μ§ννμλ€.
μνμ μ§μμ νμΈνκΈ° μν΄ κ·Έλ¦Ό μ μλ¬Όμ κ°μ²΄ μλ₯Ό νμΈν κ²°κ³Ό, μ¬μ 89κ°(M=0.96), μ¬ν 286κ°(M=3.08)λ‘ ν¬κ² μ¦κ°νμκ³ , ν΅κ³μ μΌλ‘λ μ μλ―Έν μ°¨μ΄κ° λνλ¬λ€(t=9.56. p<.001). κ·Έλ¦Ό μ μλ¬Όμ μ’
λ₯ λν μ΄ 19μ’
μμ μ΄ 31μ’
μΌλ‘ κ΄μ°°λμ΄ μνμ μ§μμ ν° ν¨κ³Όκ° μμλ€.
μνμ κ°μμ±μ νμλ€μ κ°μ μ΄μ
μ νμΈνμ¬ μΈκ°μ€μ¬μ μκ°κ³Ό μνμ€μ¬μ μκ°, κ·Έλ¦¬κ³ ν΄λΉμμμΌλ‘ λλμ΄ νμΈνμλ€. μ¬μ μ€λ¬Έμμ κ°κ° 70:20:3μΌλ‘ λνλ¬μΌλ μ¬νμλ 39:49:5μΌλ‘ λ³νν κ²μΌλ‘ 보μ μΈκ°μ€μ¬μ μκ°μ΄ μ€κ³ , μνμ€μ¬μ μκ°μ΄ λμ΄λ κ²μΌλ‘ λνλ¬λ€.
μνμ€μ¬μ νλλ νμλ€μ λ©΄λ΄μ ν΅ν΄ νμΈνμμΌλ©°, μλͺ
μ‘΄μ€κ³Ό μμ°λ³΄νΈ, μμ μμ€νκ² μ¬κΈ°λ €λ νλ λ±μμ λ³νκ° μμμμ μ μ μμλ€. κ·Έ λ°μ λ¬λΌμ§ μ μΌλ‘λ μ±μ·¨κ°μ΄ μκ²Όλ€λ μλ΅μ΄ λ€μ μμλ€.
μ΄μ κ°μ΄ ν
λ
Ό κ°κΎΈκΈ° νλμ λμ μ΄λ±νμ μνμμμ ν¨κ³Όκ° μλ κ²μΌλ‘ λνλ¬μΌλ©°, ν
λ
Ό μνκ³κ° μνκ΅μ‘μ μ μ©ν 곡κ°μμ νμΈν μ μμλ€. νΉν νμλ€μ΄ λ§€μΌ μ ν μ μλ ν
λ
Όμ νμ©νμ¬ μΌμμ μ΄κ³ μ§μμ μΈ μνκ΅μ‘μ ν λͺ¨μ΅μ μλν¨μΌλ‘μ¨ νμ μ°κ΅¬μ λν ν λλ₯Ό λ§λ ¨νλ€λ κ²μ λμ± κ°μΉκ° μλ€.β
. μλ‘ 1
1. μ°κ΅¬μ νμμ± λ° λͺ©μ 1
2. μ°κ΅¬ λ¬Έμ 2
β
‘. μ΄λ‘ μ λ°°κ²½ 3
1. μνμμ(Ecological Literacy) 3
2. λ
Ό μνκ³ 12
3. λ
Ό μνκ³ μ²΄ν νλ‘κ·Έλ¨ 19
β
’. μ°κ΅¬ λ°©λ² λ° μ μ°¨ 21
1. μ°κ΅¬ λμ 21
2. μ°κ΅¬ μ μ°¨ 22
3. ν
λ
Ό κ°κΎΈκΈ° νλ 24
4. κ²μ¬ λꡬ 32
β
£. μ°κ΅¬κ²°κ³Ό 35
1. ν
λ
Ό κ°κΎΈκΈ° νλμ μ€ν 35
2. ν
λ
Ό κ°κΎΈκΈ° νλμ΄ λμ μ΄λ±νμ μνμμμ λ―ΈμΉλ μν₯ 50
3. λ
Όμ 63
β
€. κ²°λ‘ λ° μ μΈ 65
1. κ²°λ‘ 65
2. μ μΈ 67Maste
μλΉμ€ νμ§ κ΄λ¦¬μ κ΄ν μ¬λ‘μ°κ΅¬ -Life Stylist νλλ°±νμ μ μ€μ¬μΌλ‘-
νλλ°±νμ μ μλ‘μ΄ κ°μΉμ λ―Έλμ°½μ‘°λΌλ κ²½μμ΄λ
μλ κ³ κ°μ ν볡과 μ£Όμ£Όμ ꡬμ±μμ κ°λ, μ¬νμ μ±
μμ μ΄νμ κ²½μνλμ κ·Όκ°μΌλ‘ νμ¬ μΆμ νμλ‘μμ λ§λ½ν μ μλ 21μΈκΈ° μ μν λ¬Έν μ°½μ‘°λΌλ κ²½μλͺ©νλ₯Ό κ°μ§κ³ μ΅κ³ μ μν λ° μλΉμ€λ‘ κ³ κ°κ°λμ μ€νν¨μΌλ‘μ¨ μ¬νμμ μ¬λ λ°λ κΈ°μ
, κΈ°μ¨μ μ£Όλ κΈ°μ
μ΄ λκ³ μ μ μ¬μ μΈ μλΉμ€ νμ§ κ΄λ¦¬λ₯Ό μ§μμ μΌλ‘ μ€μν¨μΌλ‘μ¨ μ΅κ³ μ λͺ
ν λ°±νμ μ΄λΌλ μμΉλ₯Ό κ³ μνκ³ μλ λ°±νμ μ΄λ€. λ³Έ μ°κ΅¬λ κ΅λ΄ λ°±νμ μ¬μ
λΆλΆμμ μ°μν μλΉμ€ νμ§ μ¬λ‘λ₯Ό 보μ¬μ€μΌλ‘μ¨ λͺ
νλ°±νμ μ νκ³ ν μμΉλ₯Ό μ°¨μ§νκ³ μλ νλλ°±νμ μ μλΉμ€ νμ§ μμ€ν
μ λν μ¬λ‘λ₯Ό λΆμνμ¬ κΈκ²©ν κ²½μνκ²½μ λ³νμ μλΉμ λ³νμ λμνκ³ μ§μ κ°λ₯ν κ²½μλ ₯ μ°μλ₯Ό μ μ§νκΈ° μν΄ μλΉμ€ νμ§μ μ΄λ»κ² κ΄λ¦¬ν΄μΌ ν μ§μ λν μμ¬μ μ λμΆν΄λ³΄κ³ μ νλ€
μ°¨κΈλ³ μ¬κ³ μ‘°μ λͺ¨νμ ν΅ν μΉμ©μ°¨ μμμ κ΄ν μ°κ΅¬
νμλ
Όλ¬Έ(μμ¬)--μμΈλνκ΅ νκ²½λνμ :νκ²½κ³ννκ³Ό κ΅ν΅κ³νμ 곡,1995.Maste
Diameter control of carbon nanotubes by changing the concentration of catalytic Fe ion solutions
Thesis(master`s)--μμΈλνκ΅ λνμ :ννλΆ λ¬Όλ¦¬ννμ 곡,2004.Maste
(An)Educational interpretation of John Dewey`s aesthetics
νμλ
Όλ¬Έ(λ°μ¬)--μμΈλνκ΅ λνμ :κ΅μ‘νκ³Ό κ΅μ‘νμ 곡,2008.2.Docto
μΌλ° νκΈ°λ¬Ό λ§€λ¦½μ§ μ μ§ μΈμ νΉμ±μ κ΄ν μ°κ΅¬
νμλ
Όλ¬Έ(μμ¬)--μμΈλνκ΅ νκ²½λνμ :νκ²½κ³ννκ³Ό,1996.Maste
μ°μ λ°μ κΈ°λ₯μ± κ΅¬μ‘°μ²΄κ° μ μλΌμ§ μ·λμ μμ‘΄μ¨κ³Ό μΈμλ¦° λΆλΉκΈ°λ₯μ λ―ΈμΉλ μν₯μ κ΄ν μ°κ΅¬
νμλ
Όλ¬Έ (λ°μ¬)-- μμΈλνκ΅ λνμ : μνκ³Ό, 2016. 8. μκ·λ¦¬.Effect of an oxygen-generating scaffold on the viability and insulin secretion function of porcine neonatal pancreatic cell clusters (NPCCs)
Eun Mi Lee
Major in Immunology, Department of Medicine
The Graduate School
Seoul National University
Diabetes is a chronic metabolic disease that occurs when the body cannot produce enough insulin or cannot use insulin and is diagnosed by observing elevated levels of glucose in the blood. Diabetes therapies can be classified into oral medication, insulin injection, and pancreas or pancreatic islet transplantation. In case of final stage of diabetes, pancreatic islet transplantation would be consider as a best therapeutic strategy. Most successful diabetes therapies through intraportal islet transplantation are considered as the Edmonton protocolhowever, pancreatic islet transplantation into the hepatic venous vessels results in induction of instant blood-mediated inflammatory reaction (IBMIR). To overcome this limitation, many researchers have tried to apply an encapsulation technique for islet transplantation. It has been widely considered as a promising method for this purpose because it blocks host antibody-mediated or cellular immune responses to the donor islets. However, it is critical to maintain the survival and function of the islets because macroencapsulation reduces the oxygen supply to the islets. Therefore, in this study, to ameliorate hypoxic damage, an oxygen-generating scaffold was used to investigate whether it can improve the viability and insulin secretion function of the islets.
Porcine neonatal pancreatic cell clusters (NPCCs) were isolated from ~5-day-old piglets and used after 7 days of culture for their maturation. A CCK-8 assay revealed that the PDMS-CaO2 scaffold group had higher viability than the other groups. In addition, the PDMS-CaO2 group showed reduced activity of apoptosis-related enzymes such as Caspase 3 and 7, which resulted in lower hypoxia-induced NPCCs death. Moreover, the reactive oxygen species (ROS) level in the PDMS-CaO2 group was lower than that in the control group. Since the goal of using oxygen-generating scaffolds is to provide supplemental oxygen for the NPCCs, the oxygen consumption rate (OCR) was determined in the NPCCs of the PDMS-CaO2 group. The PDMS-CaO2 group mostly showed higher OCR than the control group. The insulin secretion index of the NPCCs was higher in the PDMS-CaO2 group than in the PDMS or control group.
To investigate the impact of the oxygen-generating scaffolds ex vivo, a microfluidic system was established using a BioFlux 200 instrument and microfluidic device-pump system for representing the physical conditions. After injection of the NPCCs into the PDMS or PDMS-CaO2 scaffolds, they were embedded on the hole of microfluidic device (A2) and cultured under normoxic and hypoxic conditions. The viability of the NPCCs was higher in the PDMS-CaO2 group than in the PDMS group.
In addition, a biocompatibility test of the oxygen-generating scaffolds was conducted through subcutaneous implantation in mice. The scaffolds showed stability until 8 months and the immunogenicity was very low in the spleens of the recipient mice. Thus, this test can be used to confirm the viability or function of NPCCs delivered using oxygen-generating scaffolds in animal models.
This study present that NPCCs encapsulated in PDMS-CaO2 scaffolds showed higher viability and insulin secretion compared to those encapsulated in PDMS scaffolds or the controls in vitro or ex vivo. In addition, a biocompatibility test of the oxygen-generating scaffold was performed by subcutaneous implantation in mice, where the scaffold showed stability until 8 months and very low immunogenicity in the spleens of the recipient mice. Thus, the oxygen-generating scaffold has potential for application in transplantation studies in the future.1. Introduction 1
1.1. Background 1
1.2. Purpose 7
2. Materials and Methods 8
3. Results 24
3.1. Oxygen-generating scaffold fabrication 24
3.2. The effect of the oxygen-generating scaffold (in vitro) 24
3.3. Biocompatibility of oxygen-generating scaffold (in vivo) 28
3.4. Establishment of microfluidic system (ex vivo) 30
3.5. The effect of the oxygen-generating scaffold (ex vivo) 32
4. Discussion 73
5. Conclusion 78
6. References 79
Abstract in Korean 91Docto
λΌμ§ μ¨μ½λ°μ΄λ¬μ€ 2νμ κ°μΌλ μλμ μ΄μ μλ μ μ μ± μλͺ¨μ± μ¦νκ΅° λ°λ³μ λν λΌμ§μ½λ λΌ λ°±μ μ μν₯
νμλ
Όλ¬Έ(μμ¬)--μμΈλνκ΅ λνμ :μμνκ³Ό μμλ³λ¦¬ν μ 곡,2006.Maste
κΈμ΅μκΈ°μμμ μ λμ± λ¦¬μ€ν¬ κ΄λ¦¬μ μ μ© κ³΅κΈμ κ΄κ³ μ°κ΅¬
νμλ
Όλ¬Έ (μμ¬)-- μμΈλνκ΅ λνμ : κ²½μνκ³Ό, 2012. 2. κΉμμ§.λ³Έ μ°κ΅¬μμλ κ΅λ΄ κΈμ΅κΈ°κ΄ μ€ μνλ€μ΄ μΈμμ μΌλ‘ λ°μν μ λμ± μΌν¬μ μ§λ©΄νμμ λ μ λμ± λ¦¬μ€ν¬λ₯Ό κ΄λ¦¬νκΈ° μν΄ μ΄λ»κ² λμνλμ§λ₯Ό λΆμνλ€. κ°λ³ μνμ μ λμ± λ¦¬μ€ν¬ λ
ΈμΆ μ λμ λ°λΌ μμ°μ 보μ ꡬμ±μ μ΄λ»κ² μ‘°μ νλμ§, λμμ μ μ© κ³΅κΈμ μ¦κ°μ μ΄λ€ μν₯μ λ―ΈμΉλμ§ κ΄μ°°ν κ²°κ³Ό μνλ€μ 리μ€ν¬μ λ§μ΄ λ
ΈμΆλμ΄ μμμλ‘ λ΄λΆμ μ λμ±μ μκΈ° μν΄ λμΆκ³Ό μ½μ μ κ°μΆνλ κ²μ νμΈνμλ€. μ΄μ λ°λΌ μμ€μλ μ μ© κ³΅κΈ, μ¦ μ λμ± κ³΅κΈμ΄ μννμ§ μκ² λμ΄ μ λΆμ μ λμ± νλ λ
Έλ ₯μ΄ ν¬μλλ λΆλΆμ΄ μμμ κ΄μ°°νμλ€.This study analyzes how Korean banking institutions managed their liquidity risk exposure in the face of liquidity shock in the fourth quarter of 2008. I confirm that they adjusted holding of asset portfolio regarding liquid asset to build up liquidity buffer which resulted in the contraction of loans and credit supply. This result implicates that there might be a possibility that banking institutions liquidity risk management leads to an ineffective liquidity distribution against governments monetary policy in the financial crisis.Maste
- β¦