Stability of extracts from pollens of allergenic importance in Korea

BACKGROUND/AIMS: Accurate diagnosis and the effects of allergen-specific immunotherapy for pollinosis are greatly dependent on the potency and stability of the extract. This study aimed to examine factors, such as temperature and storage buffer composition, that affect the stability of allergen extracts from pollens of allergenic importance in Korea. METHODS: We prepared four pollen allergen extracts from ragweed, mugwort, Japanese hop, and sawtooth oak, which are the most important causes of seasonal rhinitis in Korea. Changes of protein and major allergen concentration were measured over 1 year by Bradford assay, two-site enzyme-linked immunosorbent assay, and sodium dodecyl sulfate-polyacrylamide gel electrophoresis after reconstitution of the lyophilized allergen extract in various buffers and stored at room temperature (RT, 18°C to 26°C) or refrigerated (4°C). RESULTS: More than 90% of the original protein concentration in all four extracts examined was detected over 1 year when 50% glycerol was added and refrigerated, whereas 57.9% to 94.5% remained in the extracts at RT. The addition of 50% glycerol to the storage buffer was found to prevent protein degradation at RT. Amb a 1, a major allergen of ragweed, was almost completely degraded in 9 weeks at RT when reconstituted in a buffer without 50% glycerol. However, 55.6% to 92.8% of Amb a 1 content was detected after 1 year of incubation at 4°C in all buffer conditions except 0.3% phenol. CONCLUSION: Addition of 50% glycerol as well as refrigeration was found to be important in increasing the shelf-life of allergen extracts from pollens of allergenic importance.ope

Optimal conditions for the storage of German cockroach extract

Allergen extracts are commonly utilized for diagnosis and immunotherapy; however, the stability of protease‑rich extracts is important for a precise diagnosis and treatment efficacy. The present study determines the optimal conditions for the storage of German cockroach allergen extract. Cockroach extracts were reconstituted in four buffers: normal saline (NS), 50% glycerol in NS, 0.3% phenol in NS, or 0.3% phenol and 50% glycerol in NS. The extracts in different buffers were stored either at room temperature (18‑26˚C, RT) or refrigerated (2‑8˚C). Subsequently, the protein concentration and allergen content (Bla g 1 and Bla g 2) in the extracts were examined for the course of one year. Extract potency was estimated by inhibition ELISA. At least 90.5% protein, 94.4% Bla g 1, 65.2% Bla g 2, and 91.4% potency remained after one year when 50% glycerol NS was added to the extract with refrigeration. However, less than 13.7% protein, 17.1% Bla g 1, 0% Bla g 2 and 32.5% potency were maintained after one year when 50% glycerol NS was not added to the extract and was maintained at RT. The addition of 0.3% phenol NS did not show significant effects on extract stability. The addition of 50% glycerol NS and refrigerated storage temperature were found to be important factors for increasing the shelf life of protease‑rich cockroach extract.restrictio

A pharmaceutical composition comprising (-)-aptosimon for prevention and treatment of inflammatory diseases

본 발명은 (-)-압토시몬[(-)-aptosimon] 또는 이의 약학적으로 허용가능한 염을 유효성분으로 포함하는 염증질환 예방 또는 치료용 약학적 조성물에 관한 것으로, 본 발명의 원화(Hibiscus syriacus 'Wonhwa')로부터 분리한 (-)-압토시몬[(-)-aptosimon]이 염증 유발에 의해 급격히 증가한 NO, PGE2(prostaglandin E2) 및 TNF-α 생성, iNOS 및 COX-2 발현 및 NF-κB 활성 억제 및 Th 2 유형 IL-4와 IL-13 사이토카인의 양을 현저하게 낮추는 효과를 가지며, 식물로부터 분리한 세포독성이 없는 안전한 물질이므로, 염증관련 질환의 예방 또는 치료를 위한 의약품, 건강기능식품 또는 기능성 사료에 유용하게 사용될 수 있다. (-)-압토시몬, 원화(Hibiscus syriacus 'Wonhwa'), 염증, NO, PGE2, TNF-α, iNOS, COX-2, NF-κB, IL-4, IL-13ope

Method for diagnosing and screening asthma using Neuropilin １

본 발명은 뉴로필린(Neuropilin, NRP) 유전자를 포함하는 천식 진단 및 스크리닝용 키트 및 이를 이용한 천식 치료제 스크리닝 방법에 관한 것으로서, 보다 구체적으로 천식 환자 및 집먼지진드기 추출물에 의해서 NRP1의 발현이 증가되며, NRP1의 발현 억제에 의해서 천식과 관련된 MMP9의 mRNA, 단백질 발현, 효소 활성 및 프로모터의 활성이 감소하는 결과를 통해서 NRP1을 천식 치료제 개발을 위한 표적으로서 유용하게 사용될 수 있음을 확인하였다.ope

Composition comprising Leuconostoc mesenteroides Ｅ―１ of having anti―allergy, anti―inflammatory and anti―asthma activity

본 발명은 항알러지, 항염증 또는 항천식 활성을 갖는 류코노스톡 메센테로이드(Leuconostoc mesenteroides) E-1을 함유하는 조성물에 관한 것으로, 구체적으로 캐피어 그레인(Kefir grain)에서 분리한 균주인 류코노스톡 메센테로이드 E-1은 난백알부민(ovalbumin)-유도 천식 동물 모델에서 기관지 폐포세척액의 사이토카인(IL-4 및 IL-13)의 생산과 호산구 증가를 억제하며, 점액분비를 억제함으로써 알러지, 염증 또는 기관지 천식 질환의 예방 및 치료를 위한 의약품 또는 건강기능식품의 유효성분으로 유용하게 이용할 수 있다. 류코노스톡 메센테로이드(Leuconostoc mesenteroides) E-1, 항알러지, 항염증, 항천식.ope

Novel steroid compound or pharmaceutically acceptable salts thereof, preparation method thereof and pharmaceutical composition for preventing and treating inflammatroy diseases containing the same as an active ingredient

본 발명은 하기 화학식 1로 표시되는 스테로이드 화합물 또는 이의 약학적으로 허용가능한 염, 이의 제조방법 및 이를 유효성분으로 함유하는 염증성 질환 예방 및 치료용 약학적 조성물에 관한 것으로, 본 발명에 따른 상기 스테로이드 화합물은 염증성 질환을 유발하는 류코트리엔의 분비 또는 생성을 효과적으로 저해함으로써, 염증, 알레르기, 천식 등의 염증성 질환을 예방 및 치료하는 데 유용하게 사용할 수 있다: [화학식 1] 염증, 알레르기, 천식, 염증성 질환, 류코트리엔, 스테로이드ope