A Method for the prevention and treatment of essential tremor by regulating a1G T―type calcium channel or by T―type calcium channel blockers

본 발명은 α1G T-타입 칼슘 채널을 억제(α1G T-type calcium channel)하여 본태성 진전증(essential tremor)을 예방 및 치료하는 방법, α1G T-타입 칼슘 채널 억제제를 유효성분으로 함유하는 본태성 진전증 예방 및 치료제, 및 α1G T-타입 칼슘 채널 억제 활성을 조사하여 본태성 진전증 예방 및 치료제의 스크리닝 방법에 관한 것으로서, 보다 상세하게는 본 발명은 α1G T-타입 칼슘 채널이 결손된(α1G -/-) 생쥐가 본태성 진전증에 대한 저항성을 가지고, 정상(α1G +/+) 생쥐에 T-타입 채널을 억제제를 투여했을 때 본태성 진전증에 저항성을 가지는 것을 확인함으로써, α1G T-타입 칼슘 채널 억제제를 이용하여 본태성 진전증을 예방 및 치료할 수 있는 방법에 관한 것이다. α1G T-타입 칼슘 채널, 본태성 진전증, α1G T-타입 칼슘 채널이 결손 된(α1G -/-) 생

Studies on the Antimicrobic Susceptibility of K. pneumoniae isolated from Doctorless Areas and Seoul Nattonal University Hospital

58 strains of K. pneumoniae isolated from residents of doctor!ess areas in Korea from 1976 to 1977 and 67 strains of K. pneumoniae isolated from patients of Seoul National University Hospital from 1975 to 1977 were examined for susceptibility to 14 antimicrobics by agar dilution method. The susceptibility of two groups to each antimicrobics were compared and incidence of resistant strains in each antimicrobics, incidence of multiple resistant strains and correlations in the antimicrobic susceptibility of 125 strains of K. pneumoniae among 14 antimicrobics were analyzed. The results obtained were as follows. 1. The mean MIC of Ampicillin, Tetracyline, Oxytetracycline, Kanamycin and Streptomycin to the K. pneumoniae isolated from patients of Seoul National University Hospital were 2. 1 to 2.8 times higher than those to the K. pneumoniae isolated from residents of doctorless areas. 2. The mean MIC of Penicillin, Doxycycline and Cotrimoxazole to the K. pneumoniae isolated from patients of Seoul National University Hospital were 1. 4 to 1. 7 times higher than those to the K. pneumoniae isolated from residents of doctorless areas. 3. Tl:ere wcre no significant differences in suscep· tibility to Carbenicillin, Cephalexin, Minocycline, Gentamicin, and Erythromycin respectively between K. pneumoniae isolated from patients of Seoul Nationa! University Hospital and those from residents of doctorless arease. 4. Incidence of resistant strains In K. pneumoniae isolated from Seoul National University Hospital was the highest with Troleandomycin (100%) and Erythromycin (100%) followed by Carbenicillin (94.0%), Penicillin (53.7%), Minocycline (46.3%), Ampicillin (44. %), Oxytetracycline (23.9%), Tetracycline(22.4 %), Doxycycline (20.9%), Streptomycin (22%), Kanamycin (16.4%), Gentamicin (7.4%), Cephalexin (1. 5%) and Cotrimoxazole (0%). 5. Frequency of resistant strain in K. pneumoniae isolated from residents of doctorless areas was the highest with Troleandomycin (100%) and Erythromycin (100%) followed by Carbenicillin (98.3%), Penicillin (44.8%), Minocycline (29.3%), Ampicillin (22.4%), Doxycycline (1. 7%), Cephal exin (1. 7%), Tetracycline (0%), Oxytetracycline (0%), Kanamycin (0%), Gentamicin (0%), Streptomycin (0%) and Cotrimoxazole (0%). There were significant differences in the incidence of resistant strains between two groups of K. pneumoniae with Ampicillin, Tetracycline, Oxytetracycline, Minecycline, Kanamycin, Gentamicin and Streptomycin (p<0.05). 6. In study with Ampicillin, Gentamicin, Kanamycin, Streptomycin. Tetracycline, Minocycline, Cephalexin, and Cotrirnoxasole, there were no multiple resistant strains in K. pneumoniae isolated from doctorless areas, while 23 strains of K. pneumoniae isolated from patients of Seoul National University Hospital showed multiple resistance. 7. There were high correlation in susceptibility of 125 strains of K. pneumoniae among Tetracycline, Oxytetracycline and Doxycycline (correlation coefficient O. 87~0. 94), but there were relatively low correlation in susceptibility between Minocycline and other 3 tetracvclines (Tetracycline, Oxytetracycline and Doxycycline). Correlation coefficient of susceptibility among penicillin analogues (Penicillin, Ampicillin and Carbenicillin) ranged between O. 30~0. 78. Correlation coefficient of susceptibility between Streptomycin and 3 tetracyclines (Tetracycline, Oxytetracycline and Doxycycline) ranged between O. 76~ 0.82. Correlation coefficient of susceptibility between Kanamycin and Streptomycin were 0.84