변화된 두부위치의 콘빔전산화단층영상에서 reorientation이 교정적 계측점 좌표에 미치는 영향

치과대학/석사본 연구의 목적은 두부위치의 변화가 교정적 진단을 위한 3차원 계측점의 좌표 값에 영향을 미치는지 알아보고 삼차원 영상분석프로그램(OnDemand 3D™)을 이용하여 보정을 시행했을 때에 좌표값의 차이가 있는지 알아보고자 한다. 건조 두개골을 이용하여 세 점에 gutta percha를 고정한 후 기준위치의 영상과 두부위치를 변화시킨 네 가지의 영상을 촬영하였다. 촬영된 다섯 개의 영상에서 기계적 원점이 자동적으로 설정된 후 20개의 교정적 계측점을 표지하고 각각의 좌표점을 계산했다. 기준위치와 변화된 두부위치 영상의 좌표점을 통계학적으로 비교하였고 영상분석프로그램(OnDemand 3D™)을 이용하여 reorientation을 시행한 후의 좌표점을 기준위치와 비교하였다. 결과는 다음과 같다. 1) reorientation 전: 기준 두부위치와 변화된 두부위치 영상의 좌표점 및 거리 사이에는 통계학적으로 유의성 있는 차이가 있었다. 2) reorientation 후: 기준 두부위치와 변화된 두부위치 영상의 reorientation 시행 후의 좌표점 및 거리 사이에는 통계학적으로 유의성 있는 차이는 없었다. 결론적으로 reorientation을 시행하기 전 두부위치의 변화는 삼차원 영상 좌표에 영향을 주었다. 그러나 3개의 gutta percha를 기준점으로 하여 영상분석 프로그램 상에서 reorientation을 시행함으로써 기준 두부위치로 정확한 정합(superimposition)이 가능하였다.ope

Prognostic implications of forkhead box protein O1 (FOXO1) and paired box 3 (PAX3) in epithelial ovarian cancer

BACKGROUND: Transcription factors forkhead box protein O1 (FOXO1) and paired box 3 (PAX3) have been reported to play important roles in various cancers. However, their role in epithelial ovarian cancer (EOC) has not been elucidated yet. Therefore, we evaluated the expression and clinical significance of FOXO1 and PAX3 in EOC. METHODS: Immunohistochemical analyses of FOXO1 and PAX3 in 212 EOCs, 57 borderline ovarian tumors, 153 benign epithelial ovarian tumors, and 79 nonadjacent normal epithelial tissues were performed using tissue microarray. Various clinicopathological variables, including the survival of EOC patients, were compared. In addition, the effect of FOXO1 on cell growth was assessed in EOC cell lines. RESULTS: FOXO1 and PAX3 protein expression levels were significantly higher in EOC tissues than in nonadjacent normal epithelial tissues, benign tissues, and borderline tumors (all p < 0.001). In EOC tissues, FOXO1 expression was positively correlated with PAX3 expression (Spearman's rho = 0.118, p = 0.149). Multivariate survival analysis revealed that high FOXO1 expression (hazard ratio = 2.77 [95% CI, 1.48-5.18], p = 0.001) could be an independent prognostic factor for overall survival. Most importantly, high expression of both FOXO1 and PAX3 showed a high hazard ratio (4.60 [95% CI, 2.00-10.55], p < 0.001) for overall survival. Also in vitro results demonstrated that knockdown of FOXO1 was associated with decreased cell viability, migration, and colony formation. CONCLUSIONS: This study revealed that high expression of FOXO1/PAX3 is an indicator of poor prognosis in EOC. Our results suggest the promising potential of FOXO1 and PAX3 as prognostic and therapeutic markers. The possible link between biological functions of FOXO1 and PAX3 in EOC warrants further studies.ope

과학계량학 지표 및 Emerging Topic 탐색 기법 연구동향보고서

비매

Heterogeneity in the Effects of FDI on Firms’ Productivity in South Korea: A Quantile Regression Approach

This study analyzes how heterogeneous across firms’ productivity level the effects of foreign direct investment (FDI) on the productivity of firms in a host country are. The study uses firm level data over 2000~2009 in South Korea and takes a quantile reⅠ. 서 론 　Ⅱ. 기존 연구 개관 　Ⅲ. 분석모형 및 데이터 　Ⅳ. 분석 결과 　Ⅳ. 연구 결과 종합 및 시사점 　참고문헌 　부

A Political Economic Analysis of Environmental Policy, Redistributive Policy, and Economic Growth

We analyse an overlapping generation model in which economic agents, especially their income distribution, influence environmental policy and redistributive policy through political decision making process. In an economic equilibrium which doesn't consideⅠ. 서 론 　Ⅱ. 관련 문헌 　Ⅲ. 이론적 분석 　Ⅳ. 요약 및 시사점 　참 고 문 헌 　부

Folic acid and vitamin B12 intake in relation to risk of endometrial cancer: Case-control study

OBJECTIVE: To evaluate the role of folic acid and vitamin B12 in the etiology of endometrial cancer, we performed a case-control study comparing oral intakes of folic acid, vitamin B12 and serum levels of folate and vitamin B12 in 48 endometrial cancer patients and 563 controls. METHODS: From August 2005 to August 2006, 48 histologically diagnosed endometrial cancer patients and 563 controls were enrolled in the study. Informations about dietary intake of folic acid, vitamin B12 were obtained by semi-quantitative food frequency questionnaire (SQFFQ) and serum levels of folate and vitamin B12 were measured. Odds ratios (OR) and 95% confidence intervals (CI) were assessed with logistic regression and adjusted for energy, age, smoking status, alcohol consumption status, BMI, menopause, oral contraceptive use. RESULTS: Endometrial cancer risk was not significantly associated with intakes of folic acid and vitamin B12. After assessing tertile subgroup levels of folic acid intake, multivariate OR of 2nd tertile subgroup was 0.71 (95% CI: 0.36~1.6) and multivariate OR of 3rd tertile subgroup was 0.92 (95% CI: 0.45~1.9). And according to tertile subgroup levels of vitamin B12 intake, multivariate OR of 2nd tertile subgroup was 1.17 (95% CI: 0.57~2.39) and multivariate OR of 3rd tertile subgroup was 0.94 (95% CI: 0.43~2.05). However, serum folate and vitamin B12 levels were significantly associated with a reduction of endometrial cancer risk as a result of multivariate OR 0.42 (95% CI: 0.22~0.78) and 0.32 (95% CI: 0.15~0.68) by logistic regression. CONCLUSIONS: This study suggests that dietary intakes of folic acid and vitamin B12 are not significantly related to the risk of endometrial cancer. But we could confirm reduction of endometrial cancer risk in higher serum folate and higher serum vitamin B12 subgroup.ope

Prevalence of Chlamydia Trachomatis and Relation with Human Papilloma Virus

Objective : One of the most common causative microorganisms in pelvic inflammatory disease (PID) is the Chlamydia trachomatis. In many cases chlamydia trachomatis infection has weak and nearly absent symptom, but it´s endocervical infection usually disseminates into upper genital tract. In this infection tubal obstruction, infertility, tubal pregnancy, and recurrent pelvic infection has involved. In this study, we investigated the prevalance of chlamydia trochoma infections in symptomatic and asymptomatic women and its relation with Human Papilloma infection. Methods : From Jan, 1999 to July, 2003, in St. Vincet´s Hospital, The Catholic University of Korea, endocervial swabs were obtained in 3416 patients (1137 in Heath Promotion Center, 2226 in OPD) by Amplicor Chlamydia Trahomatis Kits (STD swab specimen collection transprt kits). Chlamydia trachomatis DNA was extracted and amplified by PCR assays to investigate the prevalence. Also in this study we investigated the prevalence of human papilloma virus by hybrid capture method. Women who visted Hospital were received routine gynecologic examination, history taking, and physical examination and information on potential risk factors was obtained by questionnaire. Results : The prevalence rate of Chlamydia trachomatis in symptomatic women was 12.6% and there was a high peak prevalence among the early twenties (66%). The prevalence rate of Chlamydia in asymtomatic women was 8.2% but there was no peak in early twenties. In asymptomatic woman, asymptomatic Chlamydia infection can be statistically anticipated by 1) Economic status, 2) Occupation status, 3) Alcohol intake, 4) History of Gynecologic disease, 5) History of STD. Women with Chlamydia infection was 1.5 times greater risk of coincidal HPV infection and was statistically significant. Conclusion : The prevalence rate of chlamydia infection is higher than any other developed country, especially in asymptomatic women. Although symptoms are weak, the rate of prevalence and complications can be reduced by the screening of chlamydial infection.ope

치과용 지르코니아 세라믹에 대한 접착

학위논문 (박사)-- 서울대학교 대학원 : 치의과학과, 2014. 8. 조병훈.Introduction The first purpose of this study was to determine the bond strength of resin cement to zirconia ceramic after different surface treatments by means of three shear bond strength (SBS) test methods and to compare the sensitivity and the reliability of the SBS test methods. The second purpose was to evaluate the performance of the new universal adhesives (Single Bond Universal, 3M ESPE and All-Bond Universal, Bisco Inc.) as a surface treatment agent for zirconia with a test method established through the foregoing experiment. Materials and methods In Experiment I, polished zirconia ceramic (Cercon® base, DeguDent) slabs were randomly divided into four surface treatment groups: no treatment (C), airborne-particle abrasion (A), treatment with a conventional phosphate monomer-containing primer (Alloy Primer, Kuraray Co.) (P), and treatment with Alloy Primer after airborne-particle abrasion (AP). Bond strengths of resin cement to the zirconia specimens of each surface treatment group were determined by three SBS test methods: Method 1, the conventional SBS test with direct filling of the mold (Ø 4 mm x 3 mm) with resin cementMethod 2, the conventional SBS test with cementation of prefabricated composite cylinders (Ø 4 mm x 3 mm) using resin cementMethod 3, the microshear bond strength (μSBS) test with cementation of prefabricated composite cylinders (Ø 0.8 mm x 1 mm) using resin cement. The bond strength data were statistically analyzed using two-way analysis of variance (ANOVA) with factors of SBS test method and surface treatment. One-way ANOVA followed by Dunnett T3 test was performed to compare bond strengths. The coefficient of variation (CV) and the Weibull parameters were used to compare the consistency and reliability of the three test methods. In Experiment II, the performance of universal adhesives containing phosphate monomer as a surface treatment agent for zirconia was evaluated with a test method established through Experiment I and compared to that of Alloy Primer. A conventional single-bottle adhesive (Single Bond 2, 3M ESPE) was used as a negative control. Bond strengths were obtained after 24 h of water storage and after 10,000 thermocycles between 5°C and 55°C with a 25 s dwell time. The bond strength data were statistically analyzed using one-way ANOVA followed by Tukeys HSD test. Results Both SBS test method and surface treatment significantly influenced the SBS values (p < 0.05). The AP group showed the highest bond strength regardless of the test methods. Only Method 3 (μSBS test) revealed a significant difference between the P group and the A group, such that, as the SBS values increased, the CV decreased and the Weibull parameters increased. Method 3 was the most discriminative test method, producing consistent and reliable results. Method 3 was thus used to evaluate the effects of the universal adhesives on the bond strength of resin cement to zirconia. Single Bond Universal showed the highest initial bond strength (37.7 ± 5.1 MPa), followed by All-Bond Universal (31.3 ± 5.6 MPa), Alloy Primer (26.9 ± 5.1 MPa), and Single Bond 2 (8.5 ± 4.6 MPa). Artificial aging significantly reduced the bond strengths of all the test groups (p < 0.05). However, the bond strengths of Single Bond Universal (20.7 ± 6.4 MPa) and All-Bond Universal (26.9 ± 6.9 MPa) remained significantly higher than that of Alloy Primer (10.7 ± 4.2 MPa) after thermocycling (p < 0.05). Conclusions The μSBS test was more discriminative in differentiating the effects of surface treatments than the conventional SBS tests. The combination of airborne-particle abrasion and a primer containing phosphate monomer was the most effective in improving the bond strength of resin cement to zirconia. The universal adhesives significantly improved the bond strength and its durability of resin cement to zirconia when compared to Alloy Primer.Abstract Introduction Experiment I: Comparison of shear test methods to zirconia Materials and methods Results Experiment II: Shear bond strength of multi-purpose, universal adhesives to zirconia Materials and methods Results Discussion Conclusions References Tables and Figures 국문초록Docto

Reduced expression of FILIP1L, a novel WNT pathway inhibitor, is associated with poor survival, progression and chemoresistance in ovarian cancer

Filamin A interacting protein 1-like (FILIP1L) is an inhibitor of the canonical WNT pathway. WNT/β-catenin signaling and its downstream pathway, epithelial-to-mesenchymal transition (EMT), play a key role in ovarian cancer metastasis and chemoresistance. To study the clinical implications of FILIP1L in regulating the WNT/β-catenin pathway, the expression of FILIP1L, β-catenin, SNAIL and SLUG was analyzed by immunohistochemistry on tissue microarrays of 369 ovarian samples ranging from normal to metastatic. In addition, the results were validated in mouse model and in vitro cell culture. In the present study, we demonstrated that FILIP1L expression was inversely correlated with poor prognosis, stage and chemoresistance in ovarian cancer. Notably, low FILIP1L expression was independent negative prognostic factor with respect to overall and disease-free survival. FILIP1L inhibited peritoneal metastases in orthotopic mouse model. FILIP1L knockdown induced chemoresistance in ovarian cancer cells and this phenotype was rescued by simultaneous knockdown of FILIP1L and SLUG, an EMT activator. We also demonstrated that FILIP1L regulates β-catenin degradation. FILIP1L co-localizes with phospho-β-catenin and increases phospho-β-catenin at the centrosomes, destined for proteosomal degradation. Finally, we showed that FILIP1L regulates EMT. Overall, these findings suggest that FILIP1L promotes β-catenin degradation and suppresses EMT, thereby inhibiting metastases and chemoresistance. Our study provides the first clinical relevance of FILIP1L in human cancer, and suggests that FILIP1L may be a novel prognostic marker for chemotherapy in ovarian cancer patients. Further, the modulation of FILIP1L expression may have the potential to be a target for cancer therapy.ope