27 research outputs found

    Comparison of shear bond strength of orthodontic tube in glazed zirconia prostheses according to the surface treatment methods

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    ์น˜๊ณผ๋Œ€ํ•™/๋ฐ•์‚ฌCurrent studies on zirconia bond strength mostly focus on โ€œnaked zirconiaโ€, the inside of the zirconia crown. In the orthodontic field, tube bonding is required on the surface-โ€œglazedโ€ crown. In this study, shear bond strengths were measured and compared by bonding tubes not on โ€œnaked zirconiaโ€ but on โ€œglazed zirconiaโ€ using various methods. A control group was established when bonding the tube on the glazed porcelain surface using the usual methods. After glazing one side of zirconia block and dividing the experimental groups into four random groups, the tubes were bonded by using different processing methods on each of the surfaces and using different primers. Control group : 50-ยตm Al2O3 + HF + Porcelain primer Experimental group 1 : 50-ยตm Al2O3 + HF + Porcelain primer Experimental group 2 : 50-ยตm Al2O3 + Zirconia primer Experimental group 3 : 30-ยตm silica-coated alumina particles + Porcelain primer Experimental group 4 : 110-ยตm silica-coated alumina particles + Porcelain primer Shear bond strength in each of the groups was measured by using a universal test machine. Surface characteristics were observed on the glazed surface by using SEM and a 3D optical profiler prior to applying primer, and specimens were classified according to the failure patterns. The results were as follows : 1. Shear bond strength of experimental group 2 was 10.59 MPa, which was significantly lower than all other groups (p < .05). 2. Two groups, the control group and experimental group 1 were clearly observed in the SEM images to have similar rough surfaces and cracks. However, experimental groups 2, 3 and 4 were observed as having particles attached. Experimental group 4 was observed as having bigger sized particles and more irregular rough surfaces compared to experimental groups 2 and 3. In addition, according to the results of analysis using a 3D optical profiler, the average surface roughness Sa value in experimental group 3 was significantly lower than the values for experimental groups 1, 2, 4 (p .05). 3. As for the failure type of debonding surface, only adhesive failure was manifest between zirconia block surface and resin cement interface in experimental group 2. In the rest of the groups, adhesive failure between resin cement and tube base interface was mostly manifest. Therefore, this reveals a significant difference between the experimental group 2 and the rest of the groups (p < .001). According to the results of this study, using porcelain primer after sandblasting with silica coating particles is clinically simpler, safer and more effective method in relation to glazed zirconia crown because it has a similar high shear bond strength compared to other methods without the need for hydrofluoric acid.ope

    A Study on the Innovation Resistance and Usage Intention of Blockchain in the Shipping and Port field

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    Blockchain, one of the convergence technologies representing the Fourth Industrial Revolution era, is actively being introduced in the fields of finance, medical care, and logistics and distribution. Blockchain is a distributed data storage technology that stores data in blocks and connects them in chain form, replicating and storing them on numerous computers at the same time. It is also called a โ€œpublic ledger.โ€ Transaction records are sent to all the users participating in the transaction, without keeping them on a centralized server, and it is ensured that every transaction is free from data forgery or tampering by sharing the pertinent data among the users and allowing these to be compared with one another. Blockchain is also actively being promoted and introduced in the shipping and port field. In maritime transportation, which accounts for the majority of logistics transportation, the cost of handling trade-related documents is 20% of the total cost, and such task is inefficiently managed. Through the introduction of blockchain, it can be expected to become transparent and cost-cutting, with reduced disputes and increased dispute resolution cases as well as fewer cases of various types of fraud. Although blockchain has a high potential for utilization in the shipping and port field, the speed of its introduction in such field has not been accelerated as its effectiveness and safety have yet to be fully verified. Blockchain is currently known to the public only as bitcoin, and due to its vagueness, its introduction to certain fields as an innovation creates anxiety on the part of many, leading them to resist such innovation, thus hindering its wide usage. There has been insufficient research to date on innovation resistance, which serves as an obstacle to the successful introduction of blockchain in the shipping and port field, although there have been various studies of late on blockchain acceptance in academia. This study was conducted to investigate the degree and nature of the resistance to the introduction of blockchain in the shipping and port field on the part of many of its prospective users, so as to urge them to accept such innovation and to facilitate its successful introduction in the aforementioned field. This study was conducted as follows. First, the concept and characteristics of blockchain and the current status of its introduction in the shipping and port field sought to be understood through a review of the previous studies on blockchain. Second, the factors affecting the resistance to the introduction of blockchain in the shipping and port field were derived based on TAM (technology acceptance model), DOI (diffusion of innovation), the consumer characteristics theory, and the innovation resistance theory. Third, the research model to be used was empirically verified through a questionnaire survey among the workers in the shipping and port field. In this study, a research model was set up based on TAM. Seven items were selected as potential variables falling under three categories: relative benefits, compatibility, and perceived risks (variables related to the innovation characteristics); attitudes towards the existing products, innovativeness, and self-efficacy (variables related to the consumer characteristics); and cost rationality (variable related to the economic characteristics). Eight hypotheses were set and tested in this study: seven hypotheses to confirm the causal relationship between each of the aforementioned seven variables and the resistance to the introduction of blockchain in the shipping and port field, and one hypothesis to confirm the causal relationship between the said resistance and the blockchain usage intention. The survey for hypothesis testing was conducted for about 3 weeks among individuals engaged in or part of container terminal operations, shipping lines, public agencies and companies, inland transportation, international freight forwarders (3PL), and warehouse storage companies. A total of 300 questionnaires were distributed, and 239 valid accomplished questionnaires were collected. SPSS 21.0 was used for demographic analysis, and SmartPLS 3.0 was used for hypothesis testing and reliability and validity analysis. The results of this study are summarized as follows. First, the analysis of the three hypotheses on the innovation characteristics and innovation resistance showed that there was a negative (-) causal relationship between the relative advantage of introducing blockchain to the shipping and port field and the resistance to such innovation, and a positive (+) causal relationship between the perceived risk of its introduction and the resistance to such innovation. On the other hand, the causal relationship between compatibility and the innovation resistance was not verified. Second, the results of testing the three hypotheses on the consumer characteristics and innovation resistance were as follows. First, the more favorable the attitude towards the existing products was, the higher the resistance to the innovation, and the more innovative the users were, the lower their innovation resistance. On the other hand, no significant causal relationship was found between self-efficacy and innovation resistance. Third, in the case of cost rationality, a variable related to the economic characteristics, a lower degree of innovation resistance was found in the users who felt that the cost of the operation being undertaken was reduced through the introduction of blockchain. Finally, it was confirmed that the higher the innovation resistance was, the lower the intent to use the innovation. The implications of this study are as follows. First, the study is scholastically meaningful in that it deals with the two aspects of resistance and acceptance through a study of innovation resistance, a negative aspect that has not been dealt with in the previous studies related to the introduction of blockchain in the shipping and port field. In the practical aspect, this study confirmed the negative effect of innovation resistance on the intention to use the innovation, suggesting that the management of the innovation resistance is essential for the successful spread of blockchain. Therefore, efforts should be made to emphasize the functional benefits, innovativeness, and cost rationality of blockchain by establishing various channels for sharing information on it so as to control the resistance to its introduction in the shipping and port field. Also, as the perceived risks of blockchain introduction to the shipping and port field and the prospective usersโ€™ attitudes towards the existing products have been verified as negative factors for innovation resistance, it is believed that efforts should be made to come up with policies for ensuring information security, or with ways to dissolve the prospective usersโ€™ reluctance to embrace new technologies.์ดˆ ๋ก โ…ณ Abstract โ…ถ ์ œ1์žฅ ์„œ ๋ก  1 ์ œ1์ ˆ ์—ฐ๊ตฌ์˜ ํ•„์š”์„ฑ ๋ฐ ๋ชฉ์  1 ์ œ2์ ˆ ์—ฐ๊ตฌ์˜ ๋ฐฉ๋ฒ• ๋ฐ ๊ตฌ์„ฑ 4 ์ œ2์žฅ ์ด๋ก ์  ๋ฐฐ๊ฒฝ 5 ์ œ1์ ˆ ๋ธ”๋ก์ฒด์ธ ๊ธฐ์ˆ  5 ์ œ2์ ˆ ์ •๋ณด๊ธฐ์ˆ  ์‚ฌ์šฉ์˜๋„ ๊ด€๋ จ ์ด๋ก  22 ์ œ3์ ˆ ํ•ด์šดํ•ญ๋งŒ ๋ถ„์•ผ์˜ ๋ธ”๋ก์ฒด์ธ ๋„์ž…ํ˜„ํ™ฉ 48 ์ œ3์žฅ ์—ฐ๊ตฌ ์„ค๊ณ„ 53 ์ œ1์ ˆ ์—ฐ๊ตฌ๋ชจํ˜•์˜ ์„ค์ • 53 ์ œ2์ ˆ ์—ฐ๊ตฌ๊ฐ€์„ค ์„ค์ • 55 ์ œ3์ ˆ ๋ณ€์ˆ˜์˜ ์กฐ์ž‘์  ์ •์˜ ๋ฐ ์ธก์ • ๋„๊ตฌ 61 ์ œ4์ ˆ ์ž๋ฃŒ์ˆ˜์ง‘ ๋ฐ ๋ถ„์„๋ฐฉ๋ฒ• 64 ์ œ4์žฅ ์‹ค์ฆ๋ถ„์„ 65 ์ œ1์ ˆ ์—ฐ๊ตฌํ‘œ๋ณธ์˜ ํŠน์„ฑ 65 ์ œ2์ ˆ ์ธก์ • ๋„๊ตฌ์˜ ์‹ ๋ขฐ์„ฑ ๋ฐ ํƒ€๋‹น์„ฑ ๋ถ„์„ 68 ์ œ3์ ˆ ๊ฐ€์„ค ๊ฒ€์ • 73 ์ œ4์ ˆ ๋ถ„์„ ๊ฒฐ๊ณผ 76 ์ œ5์žฅ ๊ฒฐ ๋ก  79 ์ œ1์ ˆ ์—ฐ๊ตฌ๊ฒฐ๊ณผ ์š”์•ฝ ๋ฐ ์‹œ์‚ฌ์  79 ์ œ2์ ˆ ์—ฐ๊ตฌ์˜ ํ•œ๊ณ„์  ๋ฐ ํ–ฅํ›„ ์—ฐ๊ตฌ ๋ฐฉํ–ฅ 82 83 103Maste

    ์šธ๋ฆ‰ ๋ถ„์ง€์˜ ์„ฑ์ธ๊ณผ ๊ตฌ์กฐ์— ๋Œ€ํ•œ ์ค‘๋ ฅ ์ž๋ฃŒ์˜ ๋ถ„์„๊ณผ ์„ญ์ž…๋Œ€์—์„œ์˜ ์•„๋‹ค์นด์ดํŠธ ์ƒ์„ฑ์— ๊ด€ํ•œ ์ˆ˜์น˜ ๋ชจ๋ธ๋ง ์—ฐ๊ตฌ

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    ํ•™์œ„๋…ผ๋ฌธ (๋ฐ•์‚ฌ)-- ์„œ์šธ๋Œ€ํ•™๊ต ๋Œ€ํ•™์› : ์ง€๊ตฌํ™˜๊ฒฝ๊ณผํ•™๋ถ€, 2016. 2. ์ด์ƒ๋ฌต.This thesis presents the results of my studies concerning the tectonic evolution of the Ulleung Basin, East Sea using geological and geophysical data and the genesis of adakites and boninites by partial melting of oceanic crust in subduction zones. My thesis comprises two chapters and first is about the effect of time-varying subduction parameters on the Cenezoic adakites and boninites and next is the analysis of gravity measurements in the Ulleung Basin and its implications for the Moho depth variations. Chapter 1 addresses the effect of time-evolving slab age and convergence rate of the incoming oceanic plate on the genesis of adakites and boninites in the subduction zones. I made a series of two-dimensional numerical models using the time-dependent subduction parameters and compared with the geochemical evidence in each subduction zones. The results show that my model calculations successfully explained the adakites and boninites in some subduction zones. However, in the other regions such as Mariana and northeastern Japan subduction zones, the other tectonic settings of back-arc spreading, inflow of hot mantle and ridge subduction are needed to explain the partial melting of oceanic crust in subduction zones. Chapter 2 includes the analysis of gravity measurements in the Ulleung Basin and its implications for the crustal structure. I examined the gravity anomaly of the Ulleung Basin using more extensive data sets focusing on the crustal thickness and also calculated the effect of temperature on the gravity anomaly using a simple thermal model. My analysis shows that the Moho discontinuity is varied from 16 to 22 km, but within the central part of the basin, the variation is only about 10-20 %. Such finding appears to be consistent with previous studies using ocean bottom seismometers. Based on the gravity analysis, I provide the information of crustal structure and infer the important tectonic processes shaped the Ulleung Basin during post-rift period.Chapter 1 Effect of time-evolving age and convergence rate of the subducting plate on the Cenozoic adakites and boninites 10 Abstract 10 1.1. Introduction 12 1.2. Numerical model 15 1.3. Results 17 1.3.1. Izuโ€“Boninโ€“Mariana (IBM) 17 1.3.2. Northeast Japanโ€“Kuril 19 1.3.3. Tonga 20 1.3.4. Javaโ€“Sunda 21 1.3.5. Aleutians 23 1.4. Discussion 24 1.4.1. Implications of time-evolving subduction parameters to thermal structure 24 1.4.2. Other possible mechanisms responsible for adakites and boninites in arc volcanoes in the IBM, northeast Japan, Tonga, and southeastern Javaโ€“Sunda subduction zones 26 1.4.2.1. Izuโ€“Bonin 27 1.4.2.2. Mariana 28 1.4.2.3. Northeast Japan 29 1.4.2.4. Tonga 30 1.4.2.5. Southeastern Javaโ€“Sunda 31 1.4.3. Caveats: mantle potential temperature and down-dip treatment in our model calculations 32 1.5. Summary 33 1.6. References 36 1.7. Figures 46 1.8. Table 55 Chapter 2 Analysis of marine gravity anomalies in the Ulleung Basin (East Sea/Sea of Japan) and its implications for the architecture of rift-dominated backarc basin 56 Abstract 56 2.1. Introduction 58 2.2. Regional Background 60 2.3. Data and Reduction 64 2.4. Result 66 2.5. Thermal Consideration 68 2.6. Discussion 69 2.7. Conclusions 73 2.8. References 75 2.9. Figures 80 Summary (in Korean) 90Docto

    ์–๊ณผ ๋ฌด์‚ฌ์šฐ ํ•ด๊ตฌ์—์„œ์˜ ์ค‘๋ ฅ ์ด์ƒ ๊ฐ’๊ณผ ์œ ๋„๋œ ์„ญ์ž… ์ž‘์š”์— ๋Œ€ํ•œ ๊ณ ์ฐฐ

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    ํ•™์œ„๋…ผ๋ฌธ(์„์‚ฌ) --์„œ์šธ๋Œ€ํ•™๊ต ๋Œ€ํ•™์› :์ง€๊ตฌํ™˜๊ฒฝ๊ณผํ•™๋ถ€,2007.Maste

    Comparison of frictional forces between esthetic orthodonticcoated wires and self-ligation brackets

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    ์น˜์˜ํ•™๊ณผ/์„์‚ฌ์ตœ๊ทผ ๊ต์ • ์น˜๋ฃŒ์—์„œ์˜ ์‹ฌ๋ฏธ์ ์ธ ์š”๊ตฌ๊ฐ€ ์ฆ๋Œ€๋˜๋ฉด์„œ ์ฝ”ํŒ… ์™€์ด์–ด์˜ ์‚ฌ์šฉ์ด ์ ์ฐจ ๋Š˜๊ณ  ์žˆ์ง€๋งŒ, ์ฝ”ํŒ… ์™€์ด์–ด์„ ์‚ฌ์šฉํ•จ์œผ๋กœ์จ ์ƒ๊ธฐ๋Š” ๋งˆ์ฐฐ๋ ฅ ๋ณ€ํ™”์— ๋Œ€ํ•ด์„œ๋Š” ์•„์ง ์•Œ๋ ค์ง„ ๋ฐ”๊ฐ€ ์ ๋‹ค. ์ด๋ฒˆ ์—ฐ๊ตฌ์—์„œ๋Š” ์‹ฌ๋ฏธ์„ฑ์„ ์ฆ๋Œ€์‹œํ‚ค๊ธฐ ์œ„ํ•ด ๊ต์ • ์น˜๋ฃŒ ์‹œ ์ฃผ๋กœ ์‚ฌ์šฉ๋˜๋Š” ์ž๊ฐ€ ๊ฒฐ์ฐฐ ๋ธŒ๋ผ์ผ“์„ ์ด์šฉํ•˜์—ฌ, ์ฝ”ํŒ… ์™€์ด์–ด ์ค‘ polymer ์ฝ”ํŒ…๊ณผ rhodium ์ฝ”ํŒ… ์™€์ด์–ด๋ฅผ ์„ ํƒํ•˜์—ฌ ์ฝ”ํŒ…๋˜์ง€ ์•Š์€ ๊ธฐ์กด์˜ ์™€์ด์–ด์™€์˜ ๋งˆ์ฐฐ๋ ฅ์„ ์ธก์ • ๋น„๊ตํ•จ์œผ๋กœ์จ ์ž„์ƒ์ ์ธ ํšจ์šฉ์„ฑ์— ๋Œ€ํ•ด ํ‰๊ฐ€ํ•˜๊ณ ์ž ํ•˜์˜€๋‹ค. 0.016 inch NiTi, 0.017 x 0.025 inch SS ๋‘ ์ข…๋ฅ˜์˜ ์™€์ด์–ด๋ฅผ ์‚ฌ์šฉํ•˜์˜€์œผ๋ฉฐ ๋ธŒ๋ผ์ผ“๊ณผ ์™€์ด์–ด ๊ฐ„์˜ ๊ฐ๋„๋Š” 0ยฐ, 5ยฐ, 10ยฐ๋กœ ์„ค์ •ํ•˜๊ณ  Universal Testing Machine์— 0.022 inch ์Šฌ๋กฏ์˜ ์ƒ์•… ์†Œ๊ตฌ์น˜์šฉ ๋ธŒ๋ผ์ผ“(Clippy-Cยฎ, Tomy, Japan)์„ ๋ถ€์ฐฉ ํ›„ ๊ฐ๊ฐ์˜ ์™€์ด์–ด๋ฅผ ๊ณ ์ •, 5 mm/min ์†๋„๋กœ ์™€์ด์–ด ์ธ์žฅ์‹คํ—˜์„ ์‹œํ–‰ํ•˜์—ฌ ์ตœ๋Œ€ ์ •์ง€ ๋งˆ์ฐฐ๋ ฅ๊ณผ ์šด๋™ ๋งˆ์ฐฐ๋ ฅ์„ ์ธก์ •, ๋น„๊ตํ•˜์—ฌ ๋‹ค์Œ๊ณผ ๊ฐ™์€ ๊ฒฐ๊ณผ๋ฅผ ์–ป์—ˆ๋‹ค. 1.๊ต์ •์šฉ ์™€์ด์–ด๋Š” ์ฝ”ํŒ…๋˜์ง€ ์•Š์€ ์™€์ด์–ด๋ณด๋‹ค ์ฝ”ํŒ… ์™€์ด์–ด๊ฐ€ ๊ฐ™๊ฑฐ๋‚˜ ๋” ํฐ ์ตœ๋Œ€ ์ •์ง€ ๋งˆ์ฐฐ๋ ฅ, ์šด๋™ ๋งˆ์ฐฐ๋ ฅ์„ ๋‚˜ํƒ€๋‚ด์—ˆ๋‹ค (p <.05).2.์ตœ๋Œ€ ์ •์ง€ ๋งˆ์ฐฐ๋ ฅ์€ 0.016 inch NiTi ์™€์ด์–ด๋ฅผ ์‚ฌ์šฉํ•˜์—ฌ ๋ธŒ๋ผ์ผ“๊ณผ ์™€์ด์–ด ๊ฐ„ ๊ฐ๋„ 5ยฐ์ธ ์กฐ๊ฑด์—์„œ์™€, 0.017 x 0.025 inch SS ์™€์ด์–ด๋ฅผ ์‚ฌ์šฉํ•œ ๋ชจ๋“  ๊ฐ๋„์—์„œ rhodium ์ฝ”ํŒ… ์™€์ด์–ด๊ฐ€ polymer ์ฝ”ํŒ…๋ณด๋‹ค ๋” ํฐ ๋งˆ์ฐฐ๋ ฅ์„ ๋‚˜ํƒ€๋‚ด์—ˆ๋‹ค (p <.05). 3.์šด๋™ ๋งˆ์ฐฐ๋ ฅ์€ 0.016 inch NiTi ์™€์ด์–ด๋ฅผ ์‚ฌ์šฉํ•˜์—ฌ ๋ธŒ๋ผ์ผ“๊ณผ ์™€์ด์–ด ๊ฐ„ ๊ฐ๋„๊ฐ€ 0ยฐ์ผ ๋•Œ๋ฅผ ์ œ์™ธํ•œ ๋ชจ๋“  ์กฐ๊ฑด์—์„œ rhodium ์ฝ”ํŒ… ์™€์ด์–ด๊ฐ€ polymer ์ฝ”ํŒ…๋ณด๋‹ค ๋” ํฐ ๋งˆ์ฐฐ๋ ฅ์„ ๋‚˜ํƒ€๋‚ด์—ˆ๋‹ค (p <.05). 4.๊ต์ •์šฉ ์™€์ด์–ด์™€ ๋ธŒ๋ผ์ผ“ ๊ฐ„ ๊ฐ๋„๊ฐ€ ์ฆ๊ฐ€ํ• ์ˆ˜๋ก ๋” ํฐ ๋งˆ์ฐฐ๋ ฅ์ด ๋‚˜ํƒ€๋‚ฌ์œผ๋ฉฐ, 0.016 inch NiTi ์™€์ด์–ด์˜ ๊ฒฝ์šฐ 0ยฐ์—์„œ 5ยฐ๋กœ ๋ณ€ํ™”ํ•  ๋•Œ, 5ยฐ์—์„œ 10ยฐ๋กœ ๋ณ€ํ™”ํ•˜๋Š” ๊ตฌ๊ฐ„๋ณด๋‹ค 2๋ฐฐ ์ด์ƒ ํฐ ์ตœ๋Œ€ ์ •์ง€ ๋งˆ์ฐฐ๋ ฅ, ์šด๋™ ๋งˆ์ฐฐ๋ ฅ์˜ ์ฆ๊ฐ€๋Ÿ‰์„ ๋ณด์˜€๋‹ค (p <.05). ์‹ฌ๋ฏธ์„ฑ ๋ณด์™„์„ ์œ„ํ•ด ์‚ฌ์šฉ๋˜๋Š” ์ฝ”ํŒ… ์™€์ด์–ด์˜ ๋งˆ์ฐฐ๋ ฅ์ด ๊ธฐ์กด ์™€์ด์–ด๋ณด๋‹ค ๊ฐ™๊ฑฐ๋‚˜ ํฐ ๊ฒฐ๊ณผ๋ฅผ ๋‚˜ํƒ€๋‚ด์—ˆ์œผ๋‚˜ ์ž„์ƒ์ ์œผ๋กœ ์˜๋ฏธ ์žˆ๋Š” ์ฐจ์ด์ธ๊ฐ€์— ๋Œ€ํ•ด์„œ๋Š” ๊ตฌ๊ฐ• ๋‚ด์™€ ์œ ์‚ฌํ•œ ์กฐ๊ฑด์—์„œ์˜ ์ถ”๊ฐ€์ ์ธ ๋งˆ์ฐฐ๋ ฅ ์—ฐ๊ตฌ๊ฐ€ ํ•„์š”ํ•˜๋ฉฐ, ์™€์ด์–ด์˜ ์„ ํƒ์— ์žˆ์–ด์„œ๋Š” ๋งˆ์ฐฐ๋ ฅ ๋ฟ ์•„๋‹ˆ๋ผ, ์น˜์•„ ์ƒ‰์กฐ์™€์˜ ์กฐํ™”, ๋งˆ๋ชจ ์ €ํ•ญ์„ฑ, ๋ณ€์ƒ‰ ๋“ฑ ๋‹ค๋ฅธ ์š”์ธ๋“ค๋„ ๊ณ ๋ คํ•˜์—ฌ์•ผ ํ•  ๊ฒƒ์ด๋‹ค.ope

    Nutritional directed regulation of nNOS expression in the rat paraventricular nucleus

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    ์˜๊ณผํ•™๊ณผ/์„์‚ฌ[ํ•œ๊ธ€] ๋ณธ ์—ฐ๊ตฌ์—์„œ๋Š”, ์„ญ์‹์ƒํ™ฉ์— ๋”ฐ๋ฅธ ๋‡Œ์‹œ์ƒํ•˜๋ถ€ ์ธก๋‡Œ์‹คํ•ต์—์„œ์˜ nNOS ์œ ์ „์ž ๋ฐœํ˜„ ์กฐ์ ˆ์˜ ๋ถ„์ž๊ธฐ์ „์„ ์ถ”๊ตฌํ•˜์˜€๋‹ค. ์ด๋ฅผ ์œ„ํ•ด, ๊ธˆ์‹ ๋ฐ ์žฌ๊ธ‰์‹์‹œ ์ธก๋‡Œ์‹คํ•ต์˜ CREB ์ธ์‚ฐํ™” ๋ฐ nNOS ๋ฐœํ˜„ ์ˆ˜์ค€๋ณ€ํ™”๋ฅผ ๋ฉด์—ญ์กฐ์งํ™”ํ•™์  ๋ฐฉ๋ฒ•์œผ๋กœ ์กฐ์‚ฌํ•˜์—ฌ, ์ด๋“ค๊ณผ ๋‹น์งˆ์ฝ”๋ฅดํ‹ฐ์ฝ”์ด๋“œ ํ˜ˆ์ค‘ ๋†๋„ ๋ณ€ํ™” ์‚ฌ์ด์˜ ๊ด€๋ จ์„ฑ์„ ๊ฒ€ํ† ํ•˜์˜€๋‹ค. ๋˜ํ•œ, ๊ธˆ์‹์‹œ RU486ํˆฌ์—ฌ์— ์˜ํ•œ ๋‹น์งˆ์ฝ”๋ฅดํ‹ฐ์ฝ”์ด๋“œ ์ˆ˜์šฉ์ฒด ๋ด‰์‡„ํšจ๊ณผ ๋ฐ ์žฌ๊ธ‰์‹์‹œ ์—ด๋Ÿ‰ ๋ฐฐ์ œํšจ๊ณผ๋ฅผ ์กฐ์‚ฌํ•จ์œผ๋กœ์จ, ์ธก๋‡Œ์‹คํ•ต์˜ nNOS ์œ ์ „์ž ๋ฐœํ˜„ ์กฐ์ ˆ ๊ธฐ์ „์—์„œ ๋‹น์งˆ์ฝ”๋ฅดํ‹ฐ์ฝ”์ด๋“œ์˜ ์—ญํ•  ๋ฐ ์—ด๋Ÿ‰์˜ ํšจ๊ณผ๋ฅผ ๊ทœ๋ช…ํ•˜๊ณ ์ž ํ•˜์˜€๋‹ค. ๊ฒฐ๊ณผ์—์„œ ํ˜ˆ์žฅ ์ฝ”๋ฅดํ‹ฐ์ฝ”์Šคํ…Œ๋ก  ์ˆ˜์ค€์€ 48์‹œ๊ฐ„ ๊ธˆ์‹์‹œ ์œ ์˜ํ•˜๊ฒŒ ์ฆ๊ฐ€ํ•˜์˜€๊ณ , ์žฌ๊ธ‰์‹ 5์‹œ๊ฐ„ ํ›„ ๋‹ค์‹œ ๊ฐ์†Œํ•˜๋Š” ๊ฒƒ์œผ๋กœ ๊ด€์ฐฐ๋˜์—ˆ๋‹ค. ํ•œํŽธ ๊ธˆ์‹์— ์˜ํ•ด ๊ฐ์†Œ๋˜์—ˆ๋˜ nNOS์œ ์ „์ž ๋ฐœํ˜„์€ ์žฌ๊ธ‰์‹ ํ›„ ๋‹ค์‹œ ์ฆ๊ฐ€ํ•˜์˜€๋‹ค. ๋‹น์งˆ์ฝ”๋ฅดํ‹ฐ์ฝ”์ด๋“œ ์ˆ˜์šฉ์ฒด์˜ ๊ธธํ•ญ์ œ์ธ RU486์ฒ˜์น˜๋Š” ๊ธˆ์‹์— ์˜ํ•œ nNOS๋ฐœํ˜„ ๋ฐ CREB ์ธ์‚ฐํ™” ๊ฐ์†Œ๋ฅผ ๋ด‰์‡„ํ•˜์˜€๋‹ค. ์žฌ๊ธ‰์‹์‹œ, ์ •์ƒ ์‚ฌ๋ฃŒ ๊ณต๊ธ‰๊ตฐ์—์„œ๋Š” nNOS ๋ฐœํ˜„ ๋ฐ pCREB ์ˆ˜์ค€์ด ์ฆ๊ฐ€ํ•˜์˜€์œผ๋‚˜, ์—ด๋Ÿ‰์ด ๋ฐฐ์ œ๋œ ํ˜ผํ•ฉ์‚ฌ๋ฃŒ ๊ณต๊ธ‰๊ตฐ์˜ ์ธก๋‡Œ์‹คํ•ต์—์„œ๋Š” nNOS ๋ฐœํ˜„ ๋ฐ pCREB ์ˆ˜์ค€์ด ์ฆ๊ฐ€ํ•˜์ง€ ์•Š์•˜๋‹ค. ๋ฐ˜๋ฉด, ๊ธˆ์‹์œผ๋กœ ์ฆ๊ฐ€ํ–ˆ๋˜ ํ˜ˆ์žฅ ์ฝ”๋ฅดํ‹ฐ์ฝ”์Šคํ…Œ๋ก  ์ˆ˜์ค€์€ ์žฌ๊ธ‰์‹์‹œ ์—ด๋Ÿ‰์˜ ์œ ๋ฌด์— ๊ด€๊ณ„์—†์ด ๊ฐ์†Œํ•˜์˜€๋‹ค. ์ด์ƒ์˜ ๊ฒฐ๊ณผ๋กœ๋ถ€ํ„ฐ, ๊ธˆ์‹ ๋˜๋Š” ์žฌ๊ธ‰์‹์‹œ ํ˜ˆ์žฅ ๋‹น์งˆ์ฝ”๋ฅดํ‹ฐ์ฝ”์ด๋“œ๋Š” ๊ทธ์˜ ์ˆ˜์šฉ์ฒด๋ฅผ ๋งค๊ฐœ๋กœ ํ•˜์—ฌ nNOS ๋ฐœํ˜„์˜ ๋ณ€ํ™”๋ฅผ ์–ต์ œ์ ์œผ๋กœ ์กฐ์ ˆํ•˜๋ฉฐ, ์ด ๊ณผ์ •์— CREB ์ธ์‚ฐํ™”๊ฐ€ ์ˆ˜๋ฐ˜๋  ๊ฒƒ์ž„์„ ์•Œ ์ˆ˜ ์žˆ์—ˆ๋‹ค. ๋˜ํ•œ, ์ธก๋‡Œ์‹คํ•ต์˜ nNOS ์œ ์ „์ž ๋ฐœํ˜„ ์กฐ์ ˆ๊ธฐ์ „์—์„œ ์Œ์‹์œผ๋กœ๋ถ€ํ„ฐ ๊ณต๊ธ‰๋˜๋Š” ์—ด๋Ÿ‰์ด ์œ ๋„ ์ธ์ž๋กœ ๊ด€์—ฌ๋  ๊ฒƒ์ด๋ผ ์‚ฌ๋ฃŒ๋˜์—ˆ๋‹ค. ์•„์šธ๋Ÿฌ, ์„ญ์‹์ƒํ™ฉ์— ๋”ฐ๋ฅธ ํ˜ˆ์žฅ ๋‹น์งˆ์ฝ”๋ฅดํ‹ฐ์ฝ”์ด๋“œ ์ˆ˜์ค€ ๋ณ€ํ™”์—๋Š” ์—ด๋Ÿ‰ ์ด์™ธ์˜ ์„ญ์‹ ํ–‰๋™ ์ธ์ž๊ฐ€ ๊ด€์—ฌํ•  ๊ฒƒ์ž„์„ ์•Œ ์ˆ˜ ์žˆ์—ˆ๋‹ค. [์˜๋ฌธ]Nitric oxide (NO) is an important biological messenger, especially in the hypothalamic paraventricular nucleus (PVN) of the brain, NO modulates a large number of neuronal, autonomic, and endocrine functions. nNOS expression in the rat PVN is decreased by food deprivation, increased by refeeding. The plasma level of glucocorticoids increases during food deprivation and decreases shortly after refeeding. Also it was reported that adrenalectomy abolishes fasting-induced down-regulation of PVN-nNOS and dexamethasone, a synthetic glucocorticoid, down-regulates the PVN-nNOS in intact rats. It is hypothesized that glucocorticoids may negatively regulate nNOS expression depending on the feeding conditions. However, its molecular mechanism is not known yet. Recently, in vitro experiments revealed that the nNOS promoter carries cAMP response element (CRE) in its upstream and nNOS expression is mediated by a CRE-binding protein (CREB) -dependent mechanism. In this study, we firstly examined if the fasting-induced nNOS down-regulationis is mediated by glucocorticoid receptors, and then if CREB phosphorylation is implicated in this regulatory pathway, and lastly, if changes in plasma glucocorticoids level and the PVN-nNOS expression is a nutritional-directed event. In the result, nNOS positive cells and pCREB positive nucleus in the PVN significantly decreased by 48 h food deprivation, and RU486, glucocorticoid receptor antagonist, prevented this reduction. This supports the idea that the elevated level of plasma glucocorticoids during food deprivation may exert an inhibitory role in the PVN-nNOS expression, and suggests that the fasting-induced nNOS down-regulation in this brain region is mediated by glucocorticoids receptor, and furthermore, CREB phosphorylation may be implicated in the regulatory pathway of nNOS expression. Additionally, 5 h of non-caloric refeeding following after 48 h food deprivation failed to increase nNOS expression and pCREB level, which increased by 5 h of chow refeeding. However, non-caloric refeeding decreased the plasma corticosterone level, so as chow refeeding did. These results indicate that calori supplement is required to induce nNOS expression in the PVN, and suggest that plasma glucocorticoid level may be influenced by post-ingestive factors, at least, other than calori.ope

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