12 research outputs found
Morphological study of mast cells of peripheral nerve degeneration of the rat
์ํ๊ณผ/์์ฌ[ํ๊ธ]
์ ๊ฒฝ ์์ํ ์ฑ์ํ ํฐ์ฅ์ ์ฑ์ฅ์ค์ ํฐ์ฅ์ ๋น๋ง์ธํฌ ์์ ๋ณํ์ ์ด๋ ํ ์ฐจ์ด๊ฐ ์๋
์ง๋ฅผ ์์๋ณด๊ธฐ ์ํ์ฌ ์ถ์ํ 13์ผ๋ ์ฑ์ฅ์ค์ ํฐ์ฅ์ ์ฒด์ค 200 gm์ ๋์ ์ฑ์ํ ํฐ์ฅ์
์ข๊ณจ์ ๊ฒฝ์ ์์์ํค๊ณ ์์ 2์ผ, 5์ผ, 10์ผ, 1๊ฐ์, 5๊ฐ์ ํ ์์ ๋ถ์์์ ์ฒ์์ชฝ์
๊ทผ์ก์ ํต๊ณผํ ๋ถ์์ ์ข๊ณจ์ ๊ฒฝ๊ณผ ์ด ์ ๊ฒฝ์ ์์๋ถ์ธ ์ฌ๊ด์ ์งํ๋ฐฉ์ ๊ฒฝ๊ณจ์ ๊ฒฝ์ ์ ์ถ
ํ ๋ค์ 1ใ๋๊ป์ epon ์ ํธ์ ๋ง๋ค์ด ๋น๋ง์ธํฌ์ ์๋ฅผ ๊ด์ฐฐํ์ฌ ๋ค์๊ณผ ๊ฐ์ ๊ฒฐ๊ณผ๋ฅผ ์ป
์๋ค.
1. ์์ ๋ถ์์ ์์๋ถ์ธ ๊ฒฝ๊ณจ์ ๊ฒฝ์์ ์ฑ์๋ ์ฅ์ ๊ฒฝ์ฐ ์์ 1๊ฐ์๊ณผ 5๊ฐ์ ํ ๋น๋ง
์ธํฌ์ ์๊ฐ ๊ฐ๊ฐ 1.7๋ฐฐ, 4.4๋ฐฐ ์ฆ๊ฐํ์๋ค.
2. ์ฑ์ฅ์ค์ ์ฅ์์๋ ์์ 1๊ฐ์๊ณผ 5๊ฐ์ ํ ๊ฐ๊ฐ 1.3๋ฐฐ์ 1.5๋ฐฐ ์ ๋์ ์ฆ๊ฐ์ ๊ทธ์ณค
๋ค.
3. ์ ์ ์ฅ์์๋ ๋น๋ง์ธํฌ์ ์๊ฐ ํก๋จ๋ฉด์ ๊ณผ ๋ ํฐ ์๊ด๊ด๊ณ๋ฅผ ๋ณด์์ผ๋ ์์๋ ๋๋ฌผ
์์๋ ์ด๋ฌํ ๊ฒฝํฅ์ด ๋ณด์ด์ง ์์๋ค.
์ด์์ ๊ฒฐ๊ณผ๋ก ๋ฏธ๋ฃจ์ด๋ณด์ ์ ๊ฒฝ์ ๊ธฐ๊ณ์ ์ผ๋ก ์์์ํจ ๊ฒฝ์ฐ 1๊ฐ์์์ 5๊ฐ์์ด ๊ฒฝ๊ณผํ
ํ ์์ ์์๋ถ์ ๋น๋ง์ธํฌ์ ์ซ์๋ ์ฆ๊ฐํ๋ฉฐ ์ฆ๊ฐ์ ์ ๋๋ ์ฑ์๋ ์ฅ์ ๋นํด ์ฑ์ฅ์ค
์ ์ฅ์์๋ ์๋นํ ์์ ๊ฒ์ผ๋ก ๋ํ๋ฌ๋ค.
[์๋ฌธ]
Mast cells of the peripheral nerve were changed after crush injury of the adult
rat. Mast cell changes comprises early degranulation of the injured site and late
proliferation distal to the injury. However the differences between the mast cell
changes of adult rats and young rats were not yet investigated.
So we study the numerical change of mast cells of the tibial and sciatic nerver
in young and adult rats after crushing injury.
Crushing injury of the sciatic verve at greater trochanter were made on the young
rats 13 days after birth and adult rats weight around 200gm. 2 days, 5 days 10
days, 1 month, and 5 months after injury, rats were sacrificed and sciatic nerve
proximal to the lesion and tibial nerve distal to the lesion were obtained.
Specimens were embedded in epon and sectioned with ultramicrotome thickness about
1ใ and stained with toluidine blue.
Mast cell numbers were increased 1.7 times after 1 month and 4.4 times after 5
months after injury at the tibial nerve increased 1.3 and 1.7 times after 1 and 5
months respectively. The rate of increment was small in young animal than that of
the adult rat.restrictio