41 research outputs found
Expression pattern of PRDM family using tissue clearing technique in mouse micro-embryo
The proteins member of the PRDM family are known to have a potential role in tumor suppression, as well as in other diseases. Thus, elucidating the expression patterns of these PRDM proteins by high resolution analysis is necessary to future determine to characterize their biological role. Recent development in tissue clearing methods such as CLARITY (Clear Lipid-exchanged Acrylamide-hybridized Rigid Imaging/Immunostaining/In situhybridization-compatible Tissue hYdrogel) has allowed for three-dimensional analyses of biological structures as a whole, intact tissue, providing greater understanding of spatial relationships and biological circuits. Nonetheless, studies have reported issues when it comes to maintain structural integrity and preventing tissue disintegration, discouraging the application of these techniques with fragile tissues such as developing embryos. Here, we present an optimized passive clearing technique, mPACT-A (modified PACT-Acrylamide), which improves tissue rigidity without the detriment to 2 optical transparency. The mPACT-A protocol is specifically optimized for handling mouse embryos, which are small and fragile and get easily dismantled when processed with established tissue clearing methods. We demonstrated the feasibility of this technique by investigating the expression of relatively understudied PRDM proteins, PRDM7, 8, 12 and 13, in intact cleared mouse embryos at different stages of development. We observed strong PRDM7, 8, 12 and 13 expression in the developing mouse nervous system in various development stages of the mouse embryos. These results suggest potential roles for the PRDM proteins in neural development, that should be tested in future functional studies.open์
20์ ๋ฑ๋ก๊ธ๊ณผ 700๋ง์ ๋ฑ๋ก๊ธ ์ฌ์ด์์ - ๋๋(nini)์์ ์์(sรญsรญ)๋ก ๋์๊ฐ๊ธฐ
1. ์์น๋ก ๋ณด๋ ๋ฉ์์ฝ์ ๋ฑ๋ก๊ธ
๊ตญํ ๊ฐ ๋จํ๋ณด๋ค 9๋ฐฐ๋ ํฌ๊ณ , ์ธ๊ตฌ๋ 2๋ฐฐ ๋ง์ 1์ต 1์ฒ2๋ฐฑ๋ง ๋ช
์ด ์ด๊ณ ์๋ ๋๋ผ ๋ฉ์์ฝ. ๋ฉ์์ฝ ์ธ๊ตฌ ์ค ์์ 10ํผ์ผํธ์ ๋ถ์๊ฐ ๊ตญ๊ฐ ์๋์ 43.1ํผ์ผํธ๋ฅผ ์ฐจ์งํ๊ณ , ํ์ 20ํผ์ผํธ์ ๋น๊ณค ์ธ๊ตฌ๊ฐ ์๋์ 3.1ํผ์ผํธ๋ฅผ ๊ฐ์ ธ๊ฐ๋ ๋๋ผ. ์ธ๊ตฌ์ ์ ๋ฐ์ด ๋น๊ณค์ ํ๋์ด๊ณ ์ธ๊ตฌ 5๋ถ์ 1์ด์์ด ๊ทน๋น์ธต์ธ ๋๋ผ. ์ด ์ธ๊ตฌ์ 76ํผ์ผํธ๊ฐ ๋์์ ๊ฑฐ์ฃผํ๊ณ ๋ฐฑ๋ง ๋ช
์ด์์ ๋๋์ ์ํ ๊ณณ์ด ์ ์ฒด ์ธ๊ตฌ 3๋ถ์ 1์ ํฐ์ ์ด ๋๋ ๋ฉ์์ฝ. ๋์๋ก ๋ชฐ๋ฆฌ๋ ์ด๊ณณ ์ต๊ณ ์ ๋๋์์ด์ ์๋์ธ ๋ฉ์์ฝ์ํฐ์๋ ์ ๊ตญ ์ต๊ณ ์์ค์ ๊ตญยท๊ณต๋ฆฝ๋์ ์ฌ๋ฆฝ๋๊ฐ ๋ฐ์งํด ์๋ค.
2010๋
IPSOS์ฌ๊ฐ ์กฐ์ฌํ ๋ฉ์์ฝ ์ ๊ตญ ๋ํํ๊ฐ ์์ ์์ 10์๊น์ง๋ฅผ ๋ณด๋ฉด, 1์ ๋ฉ์์ฝ๊ตญ๋ฆฝ์์น๋ํ(UNAM, ์ดํ UNAM'์ผ๋ก ํ๊ธฐํจ), 2์ ๋ชฌํ
๋ ์ด ๊ณ ๋ฑ๊ณต์
๋ํ(ITESM), 3์ ๊ตญ๋ฆฝ์ฐ์
๊ธฐ์ ๋ํ(IPN), 4์ ์ด๋ฒ ๋ก์๋ฉ๋ฆฌ์นด ๋ํ(UIA), 5์ ๋ฐ์๋ฐ๋ฉ์์ฝ ๋ํ(UVM), 6์ ๋ฉ์์ฝ๊ณต์
์์น๋ํ(ITAM), 7์ ์๋์ฐ์
๋ํ, 8์ ๋ผ์ฌ์ ๋ํ, 9์ ๋ฉ์์ฝ์๋ฆฝ๋ํ(UAM), 10์ ๊ณผ๋ฌ๋ผํ๋ผ ๋ํ์ด๋ค. ์ง๋ฐฉ์ ์๋ ๊ณผ๋ฌ๋ผํ๋ผ ๋ํ์ ๋นผ๊ณ 1์๋ถํฐ 9์๊น์ง ๋ชจ๋ ๋ฉ์์ฝ์ํฐ์ ์๋ค
์ ์์ ์ฃผ์์ ์ธ๊ณํ์ `๊ตญ๋ฏผ๊ตญ๊ฐ`์ ์๋์ ์์จ์ฑ ์ฝํ : ยญํ๋์ฐจ์ค ๊ตญ๊ฐ๋ก ์ ํ์ฌ์ ํ์ฅ
ํ์๋
ผ๋ฌธ(์์ฌ)--์์ธ๋ํ๊ต ๋ํ์ :์ ์นํ๊ณผ,2006.Maste
(An)Improved method of preparing PDMS molds for nano-lithography
Thesis(master`s)--์์ธ๋ํ๊ต ๋ํ์ :์์ฉํํ๋ถ,2004.Maste
๊ณ ๋ถ์ ํธ๋์ง์คํฐ์ ๋น์ ํต์ ์ ์ ๋ฐฉ๋ฒ
Thesis(doctors) --์์ธ๋ํ๊ต ๋ํ์ :ํํ์๋ฌผ๊ณตํ๋ถ,2009.2.Docto
ํ์์ ๊ฑฐ๋ฒ๋์ค
ํ์๋
ผ๋ฌธ (์์ฌ)-- ์์ธ๋ํ๊ต ๊ตญ์ ๋ํ์ : ๊ตญ์ ํ๊ณผ(๊ตญ์ ํ๋ ฅ์ ๊ณต), 2014. 2. ์ ์ฑํธ.It is commonly held that the World Health Organization is the apex institution in the issue of global public health sector. However since the 1970s, the legitimacy and effectiveness of the WHO has continuously been questioned raising doubts about the role it was entrusted with, that is the safeguard of the highest possible level of health for all the international community. The literature of governance often diverges away from the concept of power but in realitygovernance necessarily entails an expression of power. In this thesis, a novel approach of governance has been used to analyze the relationship between power and governance of the WHO during the outbreak of SARS in 2003. Despite the prevalent neoliberalist undertone when it comes to defining the concept of governance, governance necessarily entails an expression of power.
The SARS outbreak in 2003 has been chosen to analyze what kind(s) of power the WHO had exercised for the following reasons. Externally the SARS outbreak presented itself as a newly discovered infectious disease and the impact and extent it had caused to the international community. Internally a new director-general Gro Harlem Brundtland was appointed with much expectations of reforming the WHO. This research revolves around the question whether the WHO had the necessary power or not to contain a new disease and lead the global health governance as it should. Analysis of different activities and strategies deployed by the WHO has demonstrated that despite criticisms, the WHO was indeed able to exercise a mรฉlange of governance that are institutional, structural and productive power among them productive power being the most significant one. However it is the finding of this thesis that the WHO cannot solely assume for the task of the successful containment of the SARS outbreak, in the extent that a network of myriad actors has also contributed to the containment of one hundred days of SARS. Therefore it is necessary to acknowledge the finding of this paper is limited to the case of SARS and cannot be applied generally in discussing the governance of the WHO.Table of Contents
Abstract
I. Introduction
1. Research Question
2. Literature Review
3. Hypothesis
II. Theoretical framework
1. Power and global governance : reconceptualization of power
2. Taxonomy of Power : systematic framework of analysis
3. Methodology
III. Overview of the outbreak of SARS
1. Epidemiology of SARS
2. Legal Framework for regulation of spread of infectious disease: International Health Regulations (IHR)
IV. Governance of WHO at the outbreak of SARS: a power based approach
1. Decision and Rule Making Adopted by the WHO
2. The WHO Surveillance Mechanism through GOARN
3. Assistance Provided by the WHO
V. Implications
VI. Conclusion
Bibliography
๊ตญ๋ฌธ์ด๋กMaste
๋ผํด์๋ฉ๋ฆฌ์นด ์์ ํ๊ตญ์ธ - ๋ฉ์์ฝ์ํฐ์ ํ์ธํ์ํ๋ฅผ ์ฐพ์์
๋ ์ฌ๋ฆฌ์ ํจ๊ป ๋ฉ์์ฝ์ํฐ ํ์ธํ์ํ ํ์ฅ ๋ก๋ํฌ ๊น(Rodolfo
Kim)์ ์ง์ ์ฐพ์ ๋๋ 2008๋
12์ 10์ผ ์ ๋
์ด์๋ค. ๋ฉ์์ฝ์ํฐ
๋จ์ชฝ ์์น๋ฐ์ฝ(Xochimilco)์ ์๋ ๊ทธ์ ์ง์์ ๋ค์ด์ฐ์ ๋ ๋ก๋
ํฌ์ ์๋ด ๊ฐ๋ธ๋ฆฌ์๋ผ ์๊ตฌ์์ ์นธํ
์ผ๋
ธ(Gabriela Aguayo
Cantellano), ๋ธ ์ก์ง ๊น(Song Yi Kim)์ ํ์ฌํ ํฌ๋ฆฌ์ค๋ง์ค ์ฅ์
๋ชป์ง์์ ์์์ผ๋ก ์ฐ๋ฆฌ๋ฅผ ๋ฐ๊ฒจ์ฃผ์๋ค. ๊ฐ๋จํ ์ ๋
์ ํ๋ฉด์ ์งํ
๋ ์ธํฐ๋ทฐ๋ 8์ 30๋ถ์ด ๋์ด์์ผ ๋๋ฌ๋ค. ์ฐ๋ฆฌ๋ฅผ ํ๋ํด์ค ๋ก๋
ํฌ ๊น์ ๊ฐ์กฑ๊ณผ ์ฌ์ง์ ์ฐ์ด์ค ๋ ์ฌ๋ฆฌ ๋น๋ฆฌ๋์๋ ๋ฉ๋ก ์บํฌ์ค
(Lesly Viridiana Melo Campos)์๊ฒ ๋ค์ ํ ๋ฒ ๊ฐ์ฌ๋๋ฆฐ๋ค
Development of a simulation scenario for emergency care of dyspnea for use by nurses
๊ฐํธํ๊ณผ/์์ฌ[ํ๊ธ]๋ณธ ์ฐ๊ตฌ๋ ๊ฐํธ์ฌ๋ฅผ ์ํ ํธํก๊ณค๋ ์๊ธ๊ด๋ฆฌ ์๋ฎฌ๋ ์ด์
์๋๋ฆฌ์ค๋ฅผ ๊ฐ๋ฐํ๊ธฐ ์ํ์ฌ ํธํก๊ณค๋ ์๊ธ๊ด๋ฆฌ ์๊ณ ๋ฆฌ๋ฌ์ ์์ฑํ๊ณ ์์ฑ๋ ์๊ณ ๋ฆฌ๋ฌ์ ๊ทผ๊ฑฐํ์ฌ ํธํก๊ณค๋ ์๊ธ๊ด๋ฆฌ ์๋ฎฌ๋ ์ด์
์๋๋ฆฌ์ค๋ฅผ ๊ฐ๋ฐํ๋ ๋ฐฉ๋ฒ๋ก ์ ์ฐ๊ตฌ์ด๋ค. ๊ฐ๋ฐ ๊ณผ์ ์ ํธํก๊ณค๋ ์๊ธ๊ด๋ฆฌ ์๊ณ ๋ฆฌ๋ฌ์ ์์ฑํ๊ธฐ ์ํ์ฌ ๋ฌธํ ๊ณ ์ฐฐ์ ํตํ ์๋น ์๊ณ ๋ฆฌ๋ฌ ์์ฑ, ์๊ณ ๋ฆฌ๋ฌ ๋ด์ฉํ๋น๋, ์์ํ๋น๋ ๋จ๊ณ๋ฅผ ๊ฑฐ์ณค์ผ๋ฉฐ, ์๋ฎฌ๋ ์ด์
์๋๋ฆฌ์ค๋ฅผ ์์ฑํ๊ธฐ ์ํ์ฌ ์๋๋ฆฌ์ค ์์ ์์ฑ, ๋ด์ฉํ๋น๋, ์์ ์ ์ฉ์ ๊ฑฐ์ณ ์ต์ข
์๋๋ฆฌ์ค๋ฅผ ํ์ ํ๋ ๋จ๊ณ๋ฅผ ๊ฑฐ์ณค๋ค. ๋ณธ ์ฐ๊ตฌ์ ๊ฐ๋ฐ ์ ์ฐจ์ ๋ฐ๋ฅธ ๊ฒฐ๊ณผ๋ฅผ ์์ฝํ๋ฉด ๋ค์๊ณผ ๊ฐ๋ค. 1. ํธํก๊ณค๋ ์๊ธ๊ด๋ฆฌ ์๊ณ ๋ฆฌ๋ฌ ๋จ๊ณ๋ ์ด๊ธฐ์ฌ์ , ์ฆ๊ฐ์ ์ธ ์๊ธ๊ด๋ฆฌ, ํธํก๊ณค๋ ์ฌ์ฌ์ , ํธํก๋ถ์ ๋ชจ๋ํฐ, ํธํก๋ถ์ ์ ์ฌ์ ์ง ์ฌ๋ถ ํ์ธ, ๋งฅ๋ฐ์ด ์์ ์ ์ฌํ์์์ ์ ์ํํ๊ฑฐ๋ ๋งฅ๋ฐ์ด ์ด์ง๋๋ฉด ๊ธฐ๋์ฝ๊ด์ ์ํํ๋ ๋จ๊ณ์ ํธํก์ํ ์ํ ์ ์ถ๊ฐ ์ฌ์ ์ ์ํํ์ฌ ํ์ธ์ฑ, ์ฌ์ธ์ฑ, ๊ธฐํ์์ธ์ผ๋ก ๊ฐ๋ณํ๋ 7๊ฐ์ง ๋จ๊ณ๋ก ๊ตฌ์ฑ๋์๋ค.2. ์๊ณ ๋ฆฌ๋ฌ ๋จ๊ณ ํ๋ฆ์ ์ด๊ธฐ์ฌ์ ๊ณผ ์ฆ๊ฐ์ ์ธ ์๊ธ๊ด๋ฆฌ ํ ํธํก ๊ณค๋์ ์ฌ์ฌ์ ํ์ฌ ์ํ ์ ์ถ๊ฐ์ฌ์ ์ ์ํํ ํ ์ฌ์ธ์ฑ์ผ๋ก ๊ฐ๋ณํ๋ ํ๋ฆ, ํ์ธ์ฑ์ผ๋ก ๊ฐ๋ณํ๋ ํ๋ฆ, ๊ธฐํ์์ธ์ผ๋ก ๊ฐ๋ณํ๋ ํ๋ฆ์ 3๊ฐ์ง ํ๋ฆ๊ณผ ํธํก์ํ ์
ํ ์ ํธํก๋ถ์ ์ ๋ชจ๋ํฐํ๊ณ ํธํก๋ถ์ ์ ๋งฅ๋ฐ์ ํ์ธํ์ฌ ๋งฅ๋ฐ์ด ์ด์ง๋์ง ์์ ๊ฒฝ์ฐ ์ฌํ์์์ ์ ์ํํ๋ ํ๋ฆ๊ณผ ๋งฅ๋ฐ์ด ์ด์ง ๋ ๊ฒฝ์ฐ ๊ธฐ๋์ฝ๊ด์ ์ค๋นํ๋ ํ๋ฆ์ ํฌํจํ์ฌ ์ด 5๊ฐ์ง ํ๋ฆ์ผ๋ก ๊ตฌ์ฑ๋์๋ค.3. ์๋ฎฌ๋ ์ด์
์๋๋ฆฌ์ค๋ ์๋๋ฆฌ์ค ๊ฐ์, ๊ต๊ณผ๊ณผ์ ํตํฉ, ์๋๋ฆฌ์ค ์์ ์ ์ธ๋ถ๋ถ์ผ๋ก ๊ตฌ์ฑํ์์ผ๋ฉฐ ํธํก๊ณค๋์ด ์ํ๋ ์ฌ๋กํ๋ฆ๊ณผ ํธํก๊ณค๋์ด ์
ํ๋ ์ฌ๋กํ๋ฆ์ ์์ฑํ์๋ค. ๋ณธ ์ฐ๊ตฌ์์ ๊ฐ๋ฐ๋ ํธํก๊ณค๋ ์๊ธ๊ด๋ฆฌ ์๋ฎฌ๋ ์ด์
์๋๋ฆฌ์ค๋ฅผ ํตํด ํธํก๊ณค๋ ์๊ธ๊ด๋ฆฌ ์ง์นจ์ ์ ๊ณตํ๊ณ ์๋ฎฌ๋ ์ด์
๊ต์ก์ ํตํ์ฌ ๊ฐํธ์ฌ์๊ฒ ๊ฐํธ์ง์, ์ํ๊ธฐ์ ์ ํฅ์์ํค๊ณ ๋นํ์ ์ฌ๊ณ ๊ณผ์ ์ ํตํ ๊ฐํธ๋ฅผ ์ ๊ณตํ๊ฒ ํ ๊ฒ์ด๋ค. ๋ณธ ์ฐ๊ตฌ๋ฅผ ํตํด ๊ฐ๋ฐ๋ ์๋๋ฆฌ์ค๋ฅผ ์ด์ฉํ ๊ต์ก์ ์ ์ฉํ๊ณ ๊ต์กํจ๊ณผ๋ฅผ ํ์ธํ๋ ์ฐ๊ตฌ๋ฅผ ์ ์ธํ๋ค.
[์๋ฌธ]ope
์ ๋ น๊ธฐ ์ผ๋ณธ ๋ด์ ๋๋ฆฌ์ฆ์ ์์
After World WarII, Japanese nationalism haven"t growth drastically until "90s. Because nationalism of Right Wings is too conservative to fit democracy of postwar Japan. On the other hand, nationalism of Left Wings could not fit international liberalism of those days. Mr. Hutaro Yamada -he is very famous popular writer- is one of the typical nationalist of postwar Japan. His diary written during the era of occupation regime is useful to analyze the nationalism in the period of those days. This essay looks his diary from aspects of -for example, his opinion of emperor (tenno) system, critique of the theory of the peaceful state, and criticism of Tokyo Trial. The character of his nationalism is anti-American and apathetic attitude for emperor(tennno) system. Those character were unique, but one of new case of postwar Japanese nationalisms
์ ์ ์กฐ์ ์ ๊ณ ๋ คํ ๊ณํต ์ฐ๊ณํ ํ์๊ด ๋ฐ์ ์์คํ ์ ์ด์๊ธฐ๋ฒ ์ ์
ํ์๋
ผ๋ฌธ(์์ฌ) --์์ธ๋ํ๊ต ๋ํ์ :์ ๊ธฐ. ์ปดํจํฐ๊ณตํ๋ถ,2008.2Maste