Luminescent properties of Er~(3+)/Yb~(3+) co-doped oxyfluoride glass ceramic microsphere with the tapered fiber-microsphere coupling system

采用高温煅烧法制备了Er3+/yb3+共掺的氟氧玻璃陶瓷新材料(41.2SIO2-29.4Al2O3-17.6nA2CO3-11.8lAf3-0.5Erf3-2.5ybf3),并制作了透明带柄微球.提出了用锥光纤微球耦合系统研究Er3+/yb3+共掺的氟氧玻璃陶瓷材料发光特性的新方法.它具有所需激发光功率低、制备简便和便于测试的特点.用锥光纤作为耦合器将976 nM激光高效耦合入微球,并将产生的荧光和激光耦合出微球输到光谱分析仪,测量到了强的522,545和657 nM上转换荧光,也测到Er3+产生的1562 nM激光振荡光谱图.分析了Er3+/yb3+共掺氟氧玻璃陶瓷微球中Er3+上转换发光的机理、发光效率高的机理,分析了在氟氧玻璃陶瓷微球中产生激光振荡阈值比在SIO2基质微球中高的机理.We prepare Er3+/Yb3+co-doped oxyfluoride glass ceramic samples by high temperature calcination method(41.2SiO2-29.4Al2O3-17.6Na2CO3-11.8LaF3-0.5ErF3-2.5 YbF3), and also fabricate transparent microspheres each with a handle.We propose a new method of studying the luminescent properties of Er3+/Yb3+co-doped oxyfluoride glass ceramic with the tapered fiber-microsphere coupling system.The method has characteristics such as low excitation optical power, easy preparation and testing.We couple the 976 nm laser into the microspheres with the coupler of optical tapered fiber, then the fluorescence and laser are connected to spectrum analyzer with the optical tapered fiber.The strong up-conversion fluorescences at 522, 545 and 657 nm are obtained, and the laser oscillation spectra generated by Er3+ions at 1562 nm are also measured.The up-conversion luminescence mechanism of Er3+, the mechanism of high luminescence efficiency in Er3+/Yb3+co-doped oxyfluoride glass ceramic microsphere, and the mechanism of higher laser oscillation threshold in the oxyfluoride glass ceramic microsphere than in the SiO2matrix microsphere are all analyzed

THE CLINICAL SIGNIFICANCE OF TELOMERASE ACTIVITY IN LIVER CANCER AND PRE CANCEROUS LESION

目的　探讨端粒酶检测用于早期诊断原发性肝癌的可能性。方法　采用TRAP法分别检测 2 2例原发性肝癌及癌旁组织标本、12例肝硬变组织、6例腺瘤样增生组织和 10例正常肝组织中的端粒酶活性。结果　 2 2例原发性肝癌中有 2 0例端粒酶表达阳性 ,阳性率为 90 .9% ;2 2例癌旁组织中有 14例阳性 ,阳性率为 6 3 .6 % ;12例肝硬变组织中有 10例阳性 ,阳性率为 83 .3 % ;6例腺瘤样增生组织中 5例阳性 ,阳性率为 83 .3 % ;而 10例正常肝组织的端粒酶检测均为阴性。结论　端粒酶活性表达可能发生在肝组织癌变过程中 ,并在其中扮演重要角色 ,因此端粒酶有可能成为肝癌早期诊断以及疗效和预后判断的一种标志物Objective To investigate the variety of telomerase activity in the course of liver cancer development, and the possibility of using telomerase as a marker of HCC. [WT5”HZ]Methods [WT5”BZ]Human liver specimens, comprising 22 HCC and adjacent peritumoral tissues, 12 liver cirrhosistissues, 6 nodulat regenerative hyperplasia (NRH) tissues and 10 normal liver tissues, were examined for telomerase activity by TRAP assay based on PCR. [WT5”HZ]Results [WT5”BZ]Twenty of 22 HCC and 14 of 22 adjacent tissue specimens were positive for telomerase activity with a positive rate of 90.9% and 63.6% respectively. Ten of 12 liver cirrhosis tissues were positive with a positive rate of 83.3%. 5 of 6 NRH were positive with a positive rate of 83.3%. Telomerase activity was negative in 10 normal liver tissues. [WT5”HZ]Conclusion [WT5”BZ]Telomerase may occur in the progress of hepatocarcinogenesis. Telomerase can be used as a tumor marker of HCC

硝基苯、苯胺对湿地土壤微生物和脲酶活性的影响

通过室内培养试验研究不同w(硝基苯)和w(苯胺)下,湿地土壤w(微生物量碳),土壤呼吸强度和脲酶活性的变化特征.结果表明,输入硝基苯和苯胺后,前期w(微生物量碳)明显降低.w(硝基苯)和w(苯胺)分别为10和100 mg/kg的处理前期对土壤呼吸表现为促进作用,后期表现为抑制作用;随处理浓度的提高,作用强度和作用时间有所加剧和延长.w(硝基苯)和w(苯胺)均为100 mg/kg,处理7 d后,前者对土壤呼吸的抑制作用大于后者;而w(硝基苯)为1 mg/kg的处理对土壤呼吸几乎无影响.不同w(硝基苯)的处理对土壤脲酶活性影响不显著,处理5 d后均表现为弱的激活作用;w(苯胺)为1和10 mg/kg的处理对脲酶活性一直表现为弱的激活作用;w(苯胺)为100 mg/kg的处理表现为抑制作

Study on luminescent properties of Yb~(3+)-doped phosphosilicate microsphere

本文采用双锥光纤与微球腔耦合系统研究成分为55.93P2O5-3.57Al2O3-15nA2CO3-20SIO2的掺yb3+磷硅酸盐微球腔的合作上转换发光、下转换激光振荡及其级联拉曼激光振荡等发光特性.本实验采用中心波长为976 nM、线宽为0.15 nM的单纵模半导体激光作为抽运光源,在掺yb3+磷硅酸盐微球腔中测得中心波长为476.1 nM的蓝色合作上转换荧光,并运用合适的理论模型来解释该合作上转换产生11.9 nM蓝移效应的原因.同时,在1058.26 nM和1060.02—1126.08 nM处分别测得了由于微球腔谐振产生的下转换单纵模及多纵模激光振荡.另外,本文首次在同一微球腔中测得了由yb3+下转换激光激发产生的多级级联拉曼激光.在抽运功率为8.53 MW时,产生的级联拉曼激光可以达到两级,且波长延伸至1300 nM附近.Cooperative luminescence, down-conversion laser oscillation and cascaded Raman laser in Yb3+-doped 55.93P2O5-3.57Al2O3-15Na2CO3-20SiO2 phosphosilicate glass are studied in the biconical fiber-microsphere coupling system in this paper.A single-mode semiconducter laser with the center wavelength at 976 nm and line-width 0.15 nm is used as a pump source.The blue-shifted cooperative luminescence centered at 476.1 nm is obtained in the Yb3+-doped phosphosilicate microsphere.And a suitable model may be applied to explain the reason for the 11.9 nm blue-shift in this process.Meanwhile, the single-mode laser(at 1058.26 nm) and multimode laser oscillations(from 1060.02 to 1126.08 nm) have also been obtained as the result of resonant oscillation in microsphere cavity.In addition, for the first time so far as we know a self-stimulated cascaded Raman laser is observed in the same phosphosilicate microsphere, which is generated by the down-conversion laser of Yb3+.When the pump power is 8.53 mW, a two-order cascade Raman laser is generated,which extends the laser wavelength to near 1300 nm

γ-干扰素释放试验和PPD试验在处置学校结核病暴发疫情中的应用分析

近年来,我国各地在校学生结核病的暴发疫情时有发生[1-3],给学生的身心健康造成了极大伤害。在调查处置学校结核病暴发疫情中,γ-干扰素释放试验和PPD试验作为细胞免疫学诊断结核的主要方法之一,分别通过外周血T淋巴细胞释放γ-干扰素和体内T淋巴细胞产生免疫应答来判断个体感染结核菌程度[4],为筛查发现高危密切接触者、及时控制疫情提供了科学依据。为比较γ-干扰素释放试验

超微粉体浆料惰性粒子流化床干燥过程传热传质特性

结合超微钛白粉和磁记录用钡铁氧体磁粉制备中粘性悬浮液混合浆料的脱水干燥过程,研究了在直径为145 mm的惰性粒子流化床中对悬浮液浆料进行脱水干燥的热量和质量传递的基本规律, 所得结果可为工程设计的操作参数选择提供依据, 对其他类似的超微粉体浆料的脱水干燥有参考价值