三种湿地植物抗寒性的初步研究

在阶段性降温条件下,研究了三种常用湿地植物黄鸢尾(Iris pseudacorus L.)、花菖蒲(Iris ensata Thunb.)及水芹(Oenabthe javanica)叶片抗寒性相关生理指标(可溶性蛋白质含量、可溶性糖含量、游离脯氨酸含量、丙二醛含量、超氧化物歧化酶活性、过氧化物酶活性、过氧化氢酶活性以及细胞质膜相对透性)的变化。结果表明,冷锻炼使湿地植物通过对抗氧化酶活性及渗透性的调节来提高其机体的抗寒性。低温条件下,黄鸢尾的抗氧化酶活性显著增加,5℃时SOD活性是初始值的5.77倍;花菖蒲保持较高的CAT活性,5℃时其CAT活性是黄鸢尾CAT活性的8.95倍,是水芹CAT活性的3.77倍;水芹叶片中脯氨酸含量明显提高,温度和脯氨酸含量呈负相关(R=-0.755,P=0.050)。综合比较各项生理指标可以得到这三种湿地植物的耐寒性大小顺序依次为:黄鸢尾>花菖蒲>水芹

Proteomic Analysis of Rice Cultivar Jiafuzhan in the Responses to Xanthomonas campestris pv.oryzicola Infection

作者简介: 陈芳育(1978-) , 男, 讲师。E-mail : cfy307@ sohu. com * 通讯作者(Corresponding author) : 陈亮( 1963-) , 男, 教授, 博士生导师, 研究方向: 细胞与分子生物学。E-mail: chenlg@ xmu. edu. cn[中文文摘]运用双向电泳分析高抗水稻品种“佳辐占”受强毒力细菌性条斑病病原菌侵染2d后的叶片蛋白质组变化,共发现38个蛋白质发生差异表达,其中32个上调,5个下调,1个新增。用MALDI-TOF-MS分析和数据库检索鉴定出其中的33个差异表达蛋白质,并将它们分为4个功能类群,即信号转导相关蛋白、防卫相关蛋白、代谢相关蛋白和蛋白质稳定相关蛋白。这些蛋白分别参与了信号识别、信号传递、抗氧化、糖代谢、细胞壁加固、植保素合成等抗病生理反应。研究表明,水稻对细菌性条斑病病原菌的侵染存在着一个复杂的抗病信号应答和代谢调控网络,其作用机理可以通过差异表达的蛋白质(酶)反映出来,其中差异表达的8个R蛋白和3个PR蛋白可能与水稻对细菌性条斑病的抗病性密切相关。本研究为进一步揭示水稻对细菌性条斑病的抗性机理及相关抗病基因的功能克隆提供了依据。[英文文摘]Rice bacterial leaf streak( BLS) caused by the pathogen Xanthomonas oryzae pv. oryzicola ( Xooc) is one of the major rice diseases in South China. Here we focus on proteomics as a tool for the discovery of differentially expressed proteins closely related to the disease resistance. The leaves of rice cultivar Jiafuzhan (Oryzae sativa L. ) highly resistant to the disease, were infected by"89773-1- 1" strain of the Xooc with strong pathogenicity. Total proteins were extracted from the leaves sampled at two days after inoculation, and separated by two- dimensional electrophoresis. It was found that there were thirty- eight proteins expressed differentially, of which thirty-two were up-regulated, five down-regulated and one was "new". Of the thirty- eight responsive proteins, thirty-three were identified by MALD-I TOF-MS and database searching.Based on the predicted function, we grouped them into four clusters: signal transduction, defensive responses, substance metabolism and protein stabilization, which were involved in many resistant physiological react ions, including signal recognition and transduction, antioxidant react ion, carbonhydrate metabolism, cel-l wall reinforcement and phytoalexin biosythesis. In turn a complex signal transduct ion and metabolic regulative network in the resistant responses to the infection of Xooc was outlined in this work, and the molecular mechanism was revealed by differentially expressed protein/enzyme patterns during Xooc infection. In this study, eight R proteins and three pathogenesis- related(PR) proteins which might relate closely to the disease-resistance were found. This result provides us the basic information to further reveal the resistant mechanism and conduct functional cloning of the resistan-t related genes in rice to BLS.生物农药与化学生物学教育部重点实验室( 福建农林大学) 开放课题基金项目( KF0411

Prediction of radiation pneumonitis using dose-volume histogram parameters with high attenuation in two types of cancer: A retrospective study.

The constraint values of dose-volume histogram (DVH) parameters for radiation pneumonitis (RP) prediction have not been uniform in previous studies. We compared the differences between conventional DVH parameters and DVH parameters with high attenuation volume (HAV) in CT imaging in both esophageal cancer and lung cancer patients to determine the most suitable DVH parameters in predicting RP onset. Seventy-seven and 72 patients who underwent radiation therapy for lung cancer and esophageal cancer, respectively, were retrospectively assessed. RP was valued according to the Common Terminology Criteria for Adverse Events. We quantified HAV with quantitative computed tomography analysis. We compared conventional DVH parameters and DVH parameters with HAV in both groups of patients. Then, the thresholds of DVH parameters that predicted symptomatic RP and the differences in threshold of DVH parameters between lung cancer and esophageal cancer patient groups were compared. The predictive performance of DVH parameters for symptomatic RP was compared using the area under the receiver operating characteristic curve. Mean lung dose, HAV30% (the proportion of the lung with HAV receiving ≥30 Gy), and HAV20% were the top three parameters in lung cancer, while HAV10%, HAV5%, and V10 (the percentage of lung volume receiving 10 Gy or more) were the top three in esophageal cancer. By comparing the differences in the threshold for parameters predicting RP between the two cancers, we saw that HAV30% retained the same value in both cancers. DVH parameters with HAV showed narrow differences in the threshold between the two cancer patient groups compared to conventional DVH parameters. DVH parameters with HAV may have higher commonality than conventional DVH parameters in both patient groups tested

添加固体碳源对垂直流人工湿地污水处理效果的影响

以垂直流人工湿地小试系统为研究对象，探讨了不同位置（表层、上层、中层和下层）添加固体碳源对系统氮、磷及CODcr，去除效果的影响．结果表明：湿地下层硝态氮去除率低于中层，最适宜碳源添加位置为垂直流人工湿地中、下层．添加碳源系统中，碳源添加位置为表层的系统CODcr去除率最高，各系统出水CODcr浓度均低于进水，不引起系统出水中CODcr浓度的增加．添加碳源显著提高脱氮除磷效果，碳源添加位置为下层的系统TN去除率最高，碳源添加位置为表层的系统氨氧化作用明显，出水钱态氮浓度最高，各系统对亚硝态氮和硝态氮去除率差异不明显，但对硝态氮都表现出良好的去除效果．碳源添加位置为下层的系统硝化作用最完全，TP去除能力也显著优于其他各系统．添加碳源至垂直流人工湿地下层可以达到同步脱氮除磷的效果

添加固体碳源对垂直流人工湿地污水处理效果的影响

以垂直流人工湿地小试系统为研究对象,探讨了不同位置(表层、上层、中层和下层)添加固体碳源对系统氮、磷及CODCr去除效果的影响.结果表明:湿地下层硝态氮去除率低于中层,最适宜碳源添加位置为垂直流人工湿地中、下层.添加碳源系统中,碳源添加位置为表层的系统CODCr去除率最高,各系统出水CODCr浓度均低于进水,不引起系统出水中CODCr浓度的增加.添加碳源显著提高脱氮除磷效果,碳源添加位置为下层的系统TN去除率最高,碳源添加位置为表层的系统氨氧化作用明显,出水铵态氮浓度最高,各系统对亚硝态氮和硝态氮去除率差异不明显,但对硝态氮都表现出良好的去除效果.碳源添加位置为下层的系统硝化作用最完全,TP去除能力也显著优于其他各系统.添加碳源至垂直流人工湿地下层可以达到同步脱氮除磷的效果

添加固体碳源对垂直流人工湿地污水处理效果的影响

以垂直流人工湿地小试系统为研究对象，探讨了不同位置（表层、上层、中层和下层）添加固体碳源对系统氮、磷及CODcr，去除效果的影响．结果表明：湿地下层硝态氮去除率低于中层，最适宜碳源添加位置为垂直流人工湿地中、下层．添加碳源系统中，碳源添加位置为表层的系统CODcr去除率最高，各系统出水CODcr浓度均低于进水，不引起系统出水中CODcr浓度的增加．添加碳源显著提高脱氮除磷效果，碳源添加位置为下层的系统TN去除率最高，碳源添加位置为表层的系统氨氧化作用明显，出水钱态氮浓度最高，各系统对亚硝态氮和硝态氮去除率差异不明显，但对硝态氮都表现出良好的去除效果．碳源添加位置为下层的系统硝化作用最完全，TP去除能力也显著优于其他各系统．添加碳源至垂直流人工湿地下层可以达到同步脱氮除磷的效果