Progesterone regulating glutathione S-transferase Omega-1 expression in the mouse uterine luminal and glandular epithelium during preimplantation period

目的研究谷胱甘肽S-转移酶Omega-1(Gsto 1)在小鼠胚胎着床过程中的表达和孕酮的调节。方法105只CD1小鼠,分为正常妊娠模型和类固醇激素处理模型。正常妊娠模型中,收集妊娠第1~第5天子宫,采用Real-time PCR、原位杂交和Western blotting 3种方法检测Gsto1的表达变化;类固醇激素处理模型均采用卵巢切除2周后的小鼠,又分为雌孕激素处理组、孕酮处理不同时间组和孕酮受体拮抗剂Ru486处理组,所有组中的对照均用芝麻油处理。雌孕激素处理组中,收集芝麻油、雌激素、孕酮、雌激素加孕酮分别处理12h后的子宫;孕酮处理不同时间组中,收集芝麻油和孕酮分别处理1、3、12、24 h后的子宫;Ru486处理组中,收集芝麻油、Ru486、孕酮、Ru486加孕酮分别处理12 h后的子宫。类固醇激素处理模型使用Real-time PCR和Western blotting两种方法检测Gsto1的表达变化。结果 Gsto1主要在妊娠第1~4天的子宫腔上皮及腺上皮中表达,其中,妊娠第1~3天表达量较高,第4天表达量较低,第5天着床点和非着床点均不表达。孕酮诱导Gsto1的表达,雌激素不能诱导Gsto1的表达,并能抑制孕酮对Gsto1的诱导。Ru486降低孕酮对Gsto1的诱导,孕酮处理1、3、12 h均促进Gsto1的表达,但作用24 h后,抑制Gsto1的表达。结论 Gsto1在小鼠妊娠早期子宫腔上皮及腺上皮中表达,雌激素能够拮抗孕酮对Gsto1的诱导,孕酮可以通过孕酮受体调节Gsto1的表达,并且具有短时调节作用。Objective To investigate the expression and regulation of glutathione S-transferase Omega-1( Gsto1) in the mouse uterus during embryo implantation. Methods A total of 105 CD1 mice were divided into the normal pregnancy model and steriod hormone treatment model. Uterus were collected from days 1 to 5 of pregnancy in normal pregnancy model,and Gsto1 expression was detected by Real-time PCR,in situ hybridization and Western blotting. Ovariectomized mice were used in the steriod hormone model after 2 weeks,and divided into estrogen and progesterone treatment group,progesterone treatment course group,progesterone receptor antagonist Ru486 treatment group. Sesame oil was used for the control of all groups. In the estrogen and progesterone treatment group,uterus was collected after sesame oil,estrogen,progesterone,estrogen plus progesterone treatment 12 hours,respectively. In the progesterone treatment course group,uterus was collected after progesterone treatment 1 hours,3 hours,12 hours and 24 hours,respectively. In Ru486 treatment group,uterus was collected after sesame oil,Ru486,progesterone,Ru486 plus progesterone treatment 12 hours. Gsto1 expression was detected by Real-time PCR,and Western blotting in the steriod hormone model. Results Gsto1 was mainly expressed in the uterine luminal and glandular epithelium on days 1 to 4 of pregnancy. Gsto1 expression was high on day 1 to 3,but became lower onday 4. On day 5,Gsto1 expression was not detected at implantation sites and non-implantation sites. Progesterone induced Gsto1 expression. Estrogen did not induce Gsto1 expression,but inhibited the induction of progesterone on Gsto1. Ru486 reduced the induction of progesterone on Gsto1. Progesterone treatment for 1 hour,3 hours,12 hours promoted Gsto1 expression,but after 24 hours,inhibited Gsto1 expression. Conclusion This study suggests that Gsto1 is mainly expressed in the uterine luminal and glandular epithelium during preimplantation. Estrogen inhibits the induction of progesterone on Gsto1.Progesterone enhances Gsto1 expression by progesterone receptor in short time.国家自然科学基金(31401953);; 安徽省教育厅自然科学重点项目(2014A143

MMCs的冲击力学性能及拉压不对称性研究

利用动态Hopkinson装置和Instron 4026测试了15%SiC_P/Al-5Cu、15%SiC_P/2124、15%SiC_P/Al-Li、15%SiC_W/6061和30%SiC_P/6061铝合金等几种复合材料的准静态和冲击力学性能，探讨了应变率对于强度、硬化模量和失效应变等的影响。同时观察了上述材料动态拉伸断口，揭示了金属基复合材料破坏的几种不同控制机理。最后讨论了实验中发现的上述复合材料挤压不对称性的新特征，其中考虑了金属基复合材料的热失配应力和不同的损伤、破坏控制机理

Stiffness behaviour of injection moulded short glass fibre/impact modifier/polypropylene hybrid composites

The stiffness behaviour of injection moulded short glass fibre/impact modifier/polypropylene hybrid composites has been investigated in this work by theoretical predictions and experiments. Predictions from the self-consistent method were found to be in good agreement with test results for the impact modifier/polypropylene blends. By taking into account of the fibre orientation distributions in the skin and core layers, the values of Young's modulus for the skin and core layers were predicted by employing Eshelby's equivalent inclusion method and the average induced strain approach. The prediction of the values of Young's modulus for the whole sample was obtained by applying the simple mixture theory of laminated composites to the predicted results for the skin and core layers. Good correlation between predicted and experimental Young's modulus values were found

Promotion of proliferation of luminal B breast cancer cells by mesenchymal stem cells and its underlying molecular mechanisms

目的分析人脐带间充质干细胞(hUC-MSCs)对Luminal B型乳腺癌细胞生长增殖的影响,并初步探讨其可能的分子机理。方法绿色荧光蛋白(GFP)和荧光素酶共表达慢病毒感染人Luminal B型乳腺癌细胞BT474,并经嘌呤霉素筛选两周后,于荧光显微镜下观察GFP的表达情况,IVIS Kinetic成像系统拍照以观察和记录慢病毒感染后BT474细胞荧光素酶的表达情况;荧光显微镜下直接观察,结合MTS实验分析hUC-MSCs共培养或其浓缩上清处理对GFP和荧光素酶共表达BT474细胞生长增殖的影响;Western blot法检测hUC-MSCs浓缩上清处理对BT474细胞Akt和MAPK信号通路激活情况以及下游细胞周期调控蛋白Cyclin D1表达的影响;常规RT-PCR法检测hUC-MSCs中NRG-1、NRG-2、IGF-Ⅰ、IGF-Ⅱ和EGF等配体的表达。荧光素酶表达强度与细胞数量的相关性经由Excel软件行统计学分析,MTS实验数据则经由SPSS13.0统计软件行统计学分析。结果荧光显微镜和IVIS Kinetic成像系统的观察结果分别证实,GFP和荧光素酶经慢病毒载体系统的介导可在BT474细胞中成功地共表达,且荧光素酶的表达强度与细胞数量呈直线相关。MSCs共培养或其浓缩上清处理均可显著促进Luminal B型乳腺癌细胞BT474的生长增殖,其细胞存活比例分别为各自对照组的148.06%(P<0.005)和147.99%(P<0.001);MSCs浓缩上清处理同时激活BT474细胞内Akt和MAPK信号通路,并上调细胞周期调控蛋白Cyclin D1表达。此外,RT-PCR结果显示,hUC-MSCs中NRG-1和EGF的mRNAs水平呈高表达,而NRG-2、IGF-Ⅰ和IGF-Ⅱ等配体的mRNAs表达也可见。结论 MSCs可通过表达并分泌NRG-1等配体,从而激活Luminal B型乳腺癌细胞BT474的下游Akt和MAPK信号转导通路以上调细胞周期调控蛋白Cyclin D1的表达,进而促进其生长增殖。Objective To investigate the effect of human umbilical cord mesenchymal stem cells(hUC-MSCs) on proliferation of luminal B breast cancer cells and its underlying molecular mechanisms. Methods Human luminal B breast cancer cells BT474 were infected with GFP and luciferase co-expressing lentiviruses and then subjected for selection with Puromycin for 2 weeks. The expression of GFP and luciferase was detected by fluorescent microscopy and IVIS Kinetic image system, respectively. The effect of coculture or treatment with conditioned medium of hUCMSCs on proliferation of BT474 was analyzed with MTS assay. Western blot was carried out to detect the effect of treatment with conditioned medium of hUC-MSCs on the activation of both Akt and MAPK signalings in BT474, as well as the expression of downstream cell cycle regulator Cyclin D1. Regular RT-PCR was applied to analyze the mRNAs expression of ligands such as NRG-1, NRG-2, IGF-Ⅰ, IGF-Ⅱ, and EGF in hUC-MSCs. The correlation between relative luciferase activity and cell number was analyzed with Excel software, while MTS assay data was statistically analyzed with SPSS 13.0 software. Results The co-expression of GFP and luciferase in BT474 via lentiviral expression system was visualized by fluorescent microscopy and IVIS Kinetic image system. The linear correlation between relative luciferase activity and cell number was determined by curve fitting analysis. Coculture or treatment with conditioned medium of hUC-MSC significantly promoted the proliferation of BT474, with survival rates being 148.06 %(P < 0.005)and 147.99 %(P < 0.001)of control, respectively. In addition, treatment with conditioned medium of hUC-MSC was shown to induce activation of both Akt and MAPK signalings, which further upregulated the expression of Cyclin D1. Moreover, high mRNAs expression levels of both NRG-1 and EGF, as well as moderate mRNAs expression levels NRG-2, IGF-Ⅰ, and IGF-Ⅱ were showed by RT-PCR. Conclusion Our results here demonstrated that MSCs may promote the proliferation of luminal B breast cancer cells through paracrine of ligands such as NRG-1, which in turn results in the activation of both Akt and MAPK signalings and upregulation of the expression of Cyclin D1.国家自然科学基金面上项目(81272922);; 福建省自然科学基金面上项目(2016J01577);; 福州总医院院内课题国际合作研究专项(2015G01

On laser-induced reverse plugging effect

A new kind of failure induced by long pulsed laser, named as reverse plugging effect (RPE), was experimentally observed in thin foil of brass. The whole failure process can be divided into three stages, namely thermal reverse bulging, shear deformation localization and reverse perforation. In this paper, a description of experimental and theoretical study on this newly discovered phenomenon is presented in detail

Aequorea taiwanensis n. sp. (Hydrozoa,Leptomedusae) and mtCOI sequence analysis for the genus Aequorea

【中文摘要】 Aequorea taiwanensis,a new hydrozoan species from the Taiwan Strait was described using morphological and molecular characteristics.Both morphological and mitochondrial cytochrome oxidase subunit I(mtCOI) data supported A.taiwanensis n.sp.as a valid species.Sequence divergence and genetic distance of A.taiwanensis n.sp.,A.papillata and A.conica were analysed based on the mtCOI gene sequences.The mtCOI sequences from these three species of the genus Aequorea showed high variation frequency,with sequence dive... 【英文摘要】 Aequorea taiwanensis,a new hydrozoan species from the Taiwan Strait was described using morphological and molecular characteristics.Both morphological and mitochondrial cytochrome oxidase subunit I(mtCOI) data supported A.taiwanensis n.sp.as a valid species.Sequence divergence and genetic distance of A.taiwanensis n.sp.,A.papillata and A.conica were analysed based on the mtCOI gene sequences.The mtCOI sequences from these three species of the genus Aequorea showed high variation frequency,with sequence dive..

Study on screening of oleaginous yeast and determination of intracellular lipid content by nile red dyeing

通讯作者：Tel: 86-592-2195679; :mail: [email protected][中文文摘]【目的】建立产油酵母筛选以及胞内油脂含量测定的简便方法。【方法】利用尼罗红与胞内油脂成分结合后在紫外光照射下发出荧光且荧光强弱与油脂含量相关的原理。通过在添加尼罗红的培养基中培养酵母,并观察菌落荧光的方法对385株深海酵母进行产油脂菌株筛选,利用26S rDNA D1/D2区序列分析方法对筛选获得的产油酵母菌株进行鉴定,并以其中的一株高产油脂酵母(2A00015)为试验菌株,建立了一套尼罗红染色快速测定油脂含量的方法。【结果】获得22株产油酵母,其中油脂含量最高可达62.9%,经分子鉴定后显示这22株酵母分别属于(Candida viswanathii)、近平滑假丝酵母(Candidaparapsilosis)、粘质红酵母(Rhodotorula mucilaginosa)、汉逊德巴利酵母(Debaryomyceshansenii)、季也蒙毕赤酵母(Pichia guilliermondii)以及Rhodosporidium paludigenum酵母。尼罗红染色快速测定油脂含量方法的最佳检测条件为:菌悬液OD600小于1.2,尼罗红浓度0.5 mg/L,染色时间5 min,激发波长488 nm,发射波长570 nm。该测定方法得到相对荧光强度与称重法得到油脂含量呈正相关性,R2=0.9637。[英文文摘][Objective] To establish a simple method to screen oleaginous yeast and determine the intracellular lipid content.[Methods] The study is based on the theory that the combina-tion of nile red and intracellular oil will emit fluorescence when induced by UV light and the fluoresence indensity is associated with the lipid content.We cultivate yeast in the culture medium added with nile red,and screen the oleaginous yeast strains from the 385 deep-sea yeasts by measns of obeserving the fluorescence of the yeast colony.We have identified the screened oleaginous yeast strains by the 26S rDNA D1/D2 series analysis method.Designating one of the oleaginous yeasts(2A00015) as the test strain,the lipid content rapid determination method by nile red dyeing was established.[Results] 22 oleaginous yeasts were obtained with the lipid content reaching up to 62.9%.Based on the molecular identification,it showed that the 22 yeasts are separately belong to Candida viswanathii,Candida parapailosis,Rhodotorla mucilaginosa,Debaryomyces hansenii,Pichia guilliermondii and Rhodosporidium paludi-genum.The optimum condition for lipid content determination by nile red dyeing is: bacterium suspension OD600 lower than 1.2,nile red concentration 0.5 mg/L,dyeing time 5 min,excit-ing wavelength 488 nm,emission wavelength 570 nm.The relative fluorescence intensity ob-tained by this method exhibits a positive association with the lipid content obtained by weigh-ing method,which can be explained as R2=0.9637.国家海洋局第三海洋研究所基本科研业务费专项资金项目(No.海三科2010009

OGLE-2019-BLG-1470LABc : another microlensing giant planet in a binary system?

We report the discovery and analysis of a candidate triple-lens single-source (3L1S) microlensing event, OGLE-2019-BLG-1470. This event was first classified as a normal binary-lens single-source (2L1S) event, but a careful 2L1S modelling showed that it needs an additional lens or source to fit the observed data. It is found that the 3L1S model provides the best fit, but the binary-lens binary-source (2L2S) model is only disfavoured by Δχ2 ≃ 18. All of the feasible models include a planet with planet-to-host mass-ratios 10−3 ≲ q ≲ 10−2. A Bayesian analysis based on a Galactic model indicates that the planet is super-Jovian, and the projected host-planet separation is about 3 au. Specifically, for the best-fitting 3L1S model, the two stars have masses of M1=0.57+0.43−0.32M⊙⁠, and M2=0.18+0.15−0.10M⊙ with projected separation of 1.3+0.5−0.5 au, and the planetary mass is M3=2.2+1.8−1.3MJupiter⁠. For the 2L2S model, the masses of the host star and the planet are 0.55+0.44−0.31M⊙ and 4.6+3.7−2.6MJupiter⁠, respectively. By investigating the properties of all known microlensing planets in binary systems, we find that all planets in binary systems published by the KMTNet survey are located inside the resonant caustics range with q ≳ 2 × 10−3, indicating the incompleteness of the KMTNet sample for planets in binary systems. Thus, planets in binary systems cannot be included in the current study of the KMTNet mass-ratio function, and a systematic search for planetary anomalies in KMTNet microlensing light curves of binary systems is needed

Expression, Regulation and Function of Argininosuccinate Synthetase 1 in Mouse Uterus During Peri-implantation Period

精氨酸琥珀酸合成酶（Argininosuccinatesynthetase1，Ass1）能够催化瓜氨酸（L-Citrulline）和天冬氨酸（L-Aspartate）形成精氨琥珀酸，然后在精氨琥珀酸裂解酶（Argininosuccinatelyase，Asl）的作用下，生成精氨酸（L-Arginine）和延胡索酸。由于Asl的表达量恒定，因此Ass1是生成精氨酸的限速酶。本实验利用原位杂交和Real-timePCR检测了Ass1和Asl在围着床期小鼠子宫中的表达，并利用假孕和人工诱导蜕膜化等模型研究了Ass1的表达及其调节，同时检测了在妊娠D4和D8小鼠子宫内膜和血液中的精氨酸含量。在体外细胞...Ass1 (argininosuccinate synthetase 1) is the key enzyme which converts L-Citrulline and L-Aspartate to argininosuccinic acid, which is catalyzed the breakdown to arginine and fumarate by argininosuccinate lyase (Asl). Owing to consistent expression of Asl, Ass1 is a recognized rate-limiting step in arginine synthesis. This study was to examine the expression, regulation and function of Ass1 and As...学位：理学博士院系专业：生命科学学院生物学系_动物学学号：2172008015039

Numerical Simulation on the Micro-Scale Bending Induced by Pulsed Laser Beam

利用自行研制的含热传导、冲击动力学大、变形有限元程序，模拟了小尺寸梁在脉冲激光加热条件下的变形过程。在此基础上，利用商用程序模拟了冷却及残余应力的产生，研究了激光参数（强度及分布）等对于微弯曲的影响。数值模拟结果与文献中的实验观察相吻合