Studies on cell surface proteome of key harmful algal bloom species isolated from the coast of China Sea

东海原甲藻(ProcentrumdonghaienseLu)和链状亚历山大藻(Alexandriumcatenella)是中国近海两种重要的有害赤潮生物，常形成大规模的赤潮，已对海洋生态系统、沿海养殖业和人民健康构成了严重威胁。本研究以这两种赤潮生物为模式种，运用免疫荧光标记技术、现代蛋白质组技术、免疫蛋白质组技术和生物质谱技术等研究了这两种赤潮生物细胞表面蛋白质组，制备了高特异性细胞表面抗体，分离、确认了细胞表面蛋白，并对细胞表面蛋白进行了鉴定，初步研究了赤潮生物细胞表面蛋白质组对环境中氮营养变动的响应。取得了以下主要成果：1．制备了用于识别赤潮生物细胞表面蛋白的抗体血清：0.5%多聚甲醛（...Prorocentrum donghaiense Lu and Alexandrium catenella are two key harmful algal bloom (HAB) species widely spread along the China Sea coastal waters. This two HAB species have formed extensive blomms in the coast of the China Sea in the past few years, and become of economic and public concern due to its impact on the marine ecosystem, aquaculture and public health. This study investigated the cel...学位：工学硕士院系专业：海洋与环境学院环境科学与工程系_环境科学学号：20033401

Studies on the interaction of medusa blooms and microplankton in coastal waters

水母暴发是当今严峻的生态灾害，已对生态系统造成一定的负面影响，然而其发生机理和生态环境效应尚不清楚。本文采用实验生态和现场跟踪调查等手段研究了浮游植物对海月水母（Aureliasp.1）不同生活史阶段（水螅体和碟状幼体）的行为和生长的影响，探讨了九龙江河口和厦门南湖公园以弗洲指突水母（Blackfordiavirginica）为优势种的小型水母暴发机制及生态效应；此外，通过培养实验研究了弗洲指突水母暴发对细菌代谢特征的影响，进而探讨了其对九龙江河口区碳流的影响。取得了如下主要结果： 1. 浮游植物对海月水母不同生活史阶段（水螅体和碟状幼体）行为和生长的影响 通过室内实验分析了不同浮游植物对...The burst of medusa can do harm to the health of aquatic ecosystem and become a serious environment problem, however, the underlying mechanism for their blooms is still unclear. In this study, experiments were carried out to study the effects of different phytoplankton species on medusa Aurelia sp.1 in two life stages (polys and ephyrae). For the purpose, the differences in feeding behavior, growt...学位：理学博士院系专业：海洋与环境学院_环境科学学号：2262010015361

Discussion about problems concerning design of compaction by blasting in the code

以试验数据为依据,对爆破排淤填石的机理深入分析,进而剖析爆破设计参数的内涵,并对其不足之处进行讨论,通过算例结果指出设计参数的适用情况,供工程技术人员在设计、施工时参考

Phytoplankton composition and its relationships with environmental factors at Zhangzhou section of western Jiulong River

分别在2010年的丰、平、枯3个时期,从浮游植物种类组成、生物量(叶绿素A含量)及其粒级结构等指标对九龙江漳州段浮游植物组成进行了调查,同时分析了其与环境因子间的关系,评估了该河段的水质质量。结果表明:研究区域微微型浮游植物占总生物量比例与温度存在显著的正相关性(P<0.05),小型浮游植物占总生物量比例与总氮成显著的负相关(P<0.05)。共鉴定出浮游植物7门59种,且在各时期均有差异;丰水期与枯水期相比,蓝藻比例上升(P<0.05),甲藻比例下降(P<0.05);研究水域SHAnnOn-WIEnEr指数在1.0~3.0,为中度污染。典型对应分析表明,温度和营养盐是影响九龙江西溪漳州段浮游植物群落变化的主要环境因子。From the viewpoints of species composition and biomass (size-fractionated chlorophyll a), an investigation was conducted on the phytoplankton composition at Zhangzhou section of Western Jiulong River during wet, normal, and dry seasons in 2010, with the relationships between the phytoplankton composition and environmental factors analyzed and the water quality assessed.In the study area, the proportion of picoplankton biomass to the total phytoplankton biomass had significant positive correlation with water temperature (P<0.05), and the proportion of micro-phytoplankton biomass to the total biomass had negative correlation with water total nitrogen (P<0.05).A total of 59 species belonging to 7 phyla were identified.The phytoplankton species composition and abundance varied with seasons and sites.In wet season, the proportion of Cyanobacteria increased (P<0.05), while that of Dinoflagellates decreased (P<0.05), as compared with those in dry season.Canonical correspondence analysis showed that water temperature and nutrients contents were the main environmental factors affecting the phytoplankton community composition and biomass at the Zhangzhou section of western Jiulong River.福建省教育厅科技项目(JA09164);福建省大学生科研创新实验计划;国家自然科学基金项目(40806060)资

Development of chemiluminescent microparticle immunoassay for quantitative determination of dehydroepiandrosterone sulfate

目的研制硫酸脱氢表雄酮化学发光免疫定量检测试剂。方法利用硫酸脱氢表雄酮人工完全抗原免疫小鼠,通过杂交瘤技术制备特异性抗硫酸脱氢表雄酮单克隆抗体,采用竞争抑制法建立硫酸脱氢表雄酮化学发光免疫定量检测试剂。结果筛选获得了27株稳定分泌抗硫酸脱氢表雄酮的单克隆抗体细胞株,建立了化学发光微粒子免疫法定量测定硫酸脱氢表雄酮的试剂盒雏形,与雅培公司的硫酸脱氢表雄酮定量检测试剂在检测临床标本上的相关系数r达0.99以上。结论本研究为国产化硫酸脱氢表雄酮化学发光微粒子免疫法定量测定试剂盒的研发奠定了基础。Objective To develop chemiluminescent microparticle immunoassay for quantitative determination of dehydroepiandrosterone sulfate.Methods Mice were immunized by dehydroepiandrosterone sulfate artificial complete antigen.Monoclonal antibodies against dehydroepiandrosterone sulfate were prepared by hybridoma technique.Chemiluminescent microparticle immunoassay for quantitative determination of dehydroepiandrosterone sulfate was developed based on competitive binding method.Results 27 monoclonal antibodies were obtained.Chemiluminescent microparticle immunoassay for quantitative determination of dehydroepiandrosterone sulfate prototype kit was established.The coefficient r of testing specimens with reagent from Abbott company was over 0.99.Conclusion Development of domestic chemiluminescent microparticle immunoassay for quantitative determination of dehydroepiandrosterone sulfate reagent was laid foundation by this study.国家高技术研究发展计划(863计划)(2011AA02A101

硅树脂调制的可调光子晶体

文章提出一种利用硅树脂调节光子晶体光子带隙的方法,光子晶体波导是通过往二维正方形光子晶体的介质柱之间填充硅树脂得到的,利用温度场改变硅树脂的折射率。数值模拟结果表明:通过温度场可对这种光子晶体的禁带结构进行调节。这种可调光子晶体可应用于制作新颖的偏光片和光开关

Competitive Sorp-desorption of Pb(Ⅱ) and Hg (Ⅱ) in Two Algae

研究重金属(Pb(Ⅱ)、Hg(Ⅱ))在中肋骨条藻(Skeletonema costatum)和链状亚历山大藻(Alexandrium catenella)细胞上的吸附和竞争吸附.实验结果表明,Pb(Ⅱ)、Hg(Ⅱ)在两海藻上竞争吸附时,Pb(Ⅱ)在海藻细胞表面的竞争吸附平衡快于Hg(Ⅱ).竞争吸附平衡时,藻细胞表面Hg(Ⅱ)对Pb(Ⅱ)的影响小于15%,Pb(Ⅱ)对Hg(Ⅱ)的影响大于50%.96h培养后,两金属竞争环境下的海藻细胞吸附(吸收)的重金属量明显高于单一重金属作用的海藻细胞的吸附(吸收)量.The adsorption behaviors of Pb(Ⅱ) and Hg(Ⅱ) onto two algae(Skeletonema costatum and Alexandrium catenella) with and without competing metal ions were examined in this study.In the system of all the two metals present,the interference of adsorption among the two metals is more significant: the balance time of Pb(Ⅱ) was faster than Hg(Ⅱ) while those in the competing systems.The influence efficiency of Hg(Ⅱ)to Pb(Ⅱ) was less than 15%,but Pb(Ⅱ) to Hg(Ⅱ) was more than 50%.After cultured 96h,The adsorption(uptake) of Pb and Hg onto two algae in competing system were obviously much more than those in single metal system.国家自然科学基金(40506020);; 漳州师范学院科研启动基

THE RELATIONSHIP BETWEEN QUANTITATIVE CHANGES of MICROPLANKTON AND POPULATION DYNAMICS of SMALL MEDUSA IN THE JIULONG RIVER ESTUARY IN SPRING of 2011

通过2011年春季对九龙江口的生态调查,对浮游植物、微型浮游动物以及小型水母的种类组成和数量变动特征并对分粒级叶绿素进行了研究,同时还分析了营养盐、温度和盐度等环境参数。调查共记录浮游植物种类45种,以硅藻门的中肋骨条藻(SkElETOnEMA COSTATuM)、颗粒直链藻(MElOSIrA grAnulATE)、针杆藻(SynEdrA SPP.),以及绿藻门的斜生栅藻(SCEnEdESMuS OblIQuuS)、甲藻门的微小亚历山大藻(AlEXAndrIuM MInuTuM),蓝藻门的优美平裂藻(MErISMOPEdIA ElEgAnS)具有较高的检出率;微型浮游动物分为4大类,红色中缢虫(MESOdInIuM rubruM)占有最大的比例为55.5%-79.8%,无壳纤毛虫次之,砂壳纤毛虫和无节幼体所占的比例均不足10%;小型水母种类组成中主要以弗洲指突水母(blACkfOrdIA VIrgInICA)、球型侧腕水母(PlEurObrACHIA glObOSA)、厦门和平水母(EIrEnE XIAMEnEnSIS)以及水螅水母幼体(HydrOIdOMEduSAE lArVAE)为主要优势类群,占小型水母总量的85%以上。小型水母数量在4月底和5月初达到丰度最高值(69.49±29.4)Ind/l,此时微型浮游动物数量从小型水母出现初期的峰值(1085±574.66)Ind/l下降为(526±152.93)Ind/l,同时MICrO级叶绿素占总叶绿素比例达到最大值(42.26±12.94)%。小型水母数量下降后,微型浮游动物的数量回升。在小型水母数量消长过程中,浮游植物、微型浮游动物和小型水母数量间处于动态平衡。The abundances and compositions of phytoplankton,microzooplankton and small medusa were investigated in the Jiulong River Estuary in spring of 2011,meanwhile environmental factors such as nutrients,temperature,and salinity were measured.Totally 45 species of phytoplankton were recorded,and Skeletonema costatum,Melosira granulate,Synedra spp,Scenedesmus obliquus,Alexandrium minutum,and Merismopedia elegans showed high detection rate.Microzooplankton contained four classes,in which Mesodinium rubrum was the most common species,accounting for 55.5%—79.8% of the total population,aloricate ciliates were second;and loricate ciliates and nauplius were less than 10%.The total abundances of Blackfordia virginica,Pleurobrachia globosa,Eirene xiamenensis,and Hydroidomedusae larvae dominated 85% of small medusa species.There was a peak value of small medusa abundances in late April and early May,up to(69.49±29.4)ind/L,and the quantity of microzooplankton decreased from(1085±574.66)ind/L to(526±152.93)ind/L,while the percentage of micro-chlorophyll a in bulk chlorophyll a was(42.26±12.94)%.Then,the abundances of microzooplankton increased alone with the decreasing numbers of small medusa.The abundances of phytoplankton,microzooplankton,and small medusa were in a dynamic equilibrium during the spring period in this estuary.国家重点基础研究发展计划(973)资助项目;2011CB403603号;国家自然科学基金项目;40806060号;福建省教育厅科技项目;JA09164

The HAR-RV-EMD-J Model and Its Application to Forecasting the Volatility of Financial Assets

本文在HAR-RV模型的基础上,运用EMD等方法将模型中的已实现波动率分解成高频已实现波动率、低频已实现波动率和趋势已实现波动率,并加入跳跃波动率成分,构建HARRV-EMD-J模型;接着,以沪深300股指和沪深300股指期货的5分钟高频交易数据为实证样本,对HAR-RV-EMD-J模型以及常见的四个HAR类波动率模型进行样本内分析和样本外分析,并对其分析结果进行稳健性检验。研究发现：在HAR-RV-EMD-J模型中,高频已实现波动率和低频已实现波动率包含对未来1日、1周、2周和1月波动率的预测信息较多,而趋势已实现波动率和跳跃波动率包含的预测信息较少;HAR-RV-EMD-J模型对未来1日、1周、2周和1月波动率的样本内和样本外预测能力都明显强于其他四个HAR类波动率模型。On the basis of the HAR-RV model,we develop a new HAR-type volatility model（ i.e.,HAR-RV-EMD-J model）. Then we use 5-min high-frequency transaction data of the CSI 300 stock index and CSI 300 stock index futures as the study sample,and respectively analyzes the HAR-RV-EMD-J and the other four HAR-type volatility models. The results indicate that the high-frequency realized volatility,low-frequency realized volatility can be used to predict the future 1 day,1 week,2 week and 1 month volatilities,while the trend realized volatility and jump volatility are poor at its prediction accuracy. Besides,the HAR-RV-EMD-J model shows obviously better forecasting performance than the other HAR-type volatility models on forecasting the future volatilities of the CSI 300 stock index and CSI 300 stock index futures.国家自然科学基金项目（71371195;71431008;71471020;71633006）; 国家社会科学基金重大项目（14ZDA045）; 中南大学中央高校基本科研业务费专项资金资助（2015zzts006