肾移植术后淋巴漏及淋巴囊肿的诊治

[目的] 探讨肾移植术后淋巴漏或淋巴囊肿的诊断及治疗方法。[方法]回顾性分析21例肾移植术后淋巴漏（或囊肿）患者1，对淋巴漏或囊肿的可能原因进行探讨并通过引流液生化检查、乳靡试验和B超检查，分为非手术治疗和手术治疗。对诊断和治疗方法进行总结。[结果]21例中，17例为淋巴漏，4例为淋巴囊肿。6例单纯引流后治愈；15例经引流管注入无水酒精；其中9例治愈．6例治疗无效，最后经开放手术治愈。本组病例中，3例淋巴漏伴有感染，1例淋巴漏伴有移植肾急性排斥．经抗排斥治疗好转，淋巴漏治愈。[结论]肾移植术后淋巴漏或淋巴囊肿多与手术操作欠仔细或术中漏扎小淋巴管有关；多数病人可经非手术治疗痊愈，少数病人尤其淋巴囊肿形成者需开放手术治疗

The preparation of ZnSe Qdots in aqueous solution and the effects of photo-induced fluorescence enhancement

在水相中,以巯基乙酸(TgA)为稳定剂制备了具有短波长荧光的znSE量子点.研究了znSE量子点光诱导荧光增敏的机理,并提出通过补加zn2+和TgA以提高光诱导荧光增敏效率以及所得znSE量子点的稳定性这一新思路.研究结果表明,提高补加的zn2+和TgA的量即可增加znSE量子点表面znS壳层的厚度,更好地钝化其表面,从而不仅可显著提高znSE量子点的荧光量子产率(最高可接近15%),而且可大大地提高其表面的抗氧化性和荧光稳定性.ZnSe Qdots with short wavelength-fluorescence were prepared in aqueous solution with thioglycolic acid(TGA) as capping reagent.The mechanism of photo-induced fluorescence enhancement was studied and it was proposed a novel approach involved in the addition of compensatory Zn2+ ions and TGA to the original ZnSe solution to increase the efficiency of photo-induced fluorescence enhancement and stability of ZnSe Qdots.The research indicated that the thickness of ZnS shell increases with the increase of the quantity of Zn2+ ions and TGA.The ZnS shell made a better passivation of the ZnSe Qdot surface,thus resulted in not only higher fluorescence quantum yield(maximal 15%),but also higher stability of ZnSe Qdots.It was expected that the proposed approach would also provide a novel route to increase the fluorescence quantum yield and stability of other types of Qdot prepared in aqueous solution.浙江省自然科学基金资助项目(Y4080518

戊型肝炎病毒ORF1多聚蛋白的不同功能域在细胞内的定位

为了探讨戊型肝炎病毒多聚蛋白ORF1的多个功能域在宿主细胞中的表达和定位情况,我们首先将psk-HEV重组载体上的ORF1各功能域的编码序列克隆到绿色荧光蛋白载体pcDNA3.1-GFP上,构建成融合表达的重组质粒,并测序和酶切鉴定其构建成功。再通过Western-Blot验证各融合蛋白在细胞中正确表达,并用激光扫描共聚焦显微镜观察融合蛋白在细胞内的分布和定位。在Huh7细胞中,RdRp蛋白主要分布于细胞核内,HEL蛋白以囊泡状分布于细胞核周,MET蛋白以颗粒状存在于细胞核和细胞质中,PLP蛋白呈极性分布于细胞核周,X蛋白在细胞核和细胞质中均存在。各融合蛋白在细胞中的不同定位印证了对这些蛋白质的功能预测和体外研究结果,这为进一步研究HEV不同蛋白功能提供了支持